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Neural Regeneration Research 2016年第10期11卷 1653-1659页

作者： Xiao-pan Wang Min Wu jian-zhong Guan Zhao-dong Wang Xu-bin Gao yang-yang Liu Department of Orthopedics Bengbu Medical University Affiliated to First Hospital

Schwann cells play an important role in the peripheral nervous system, especially in nerve repair following injury, so artificial nerve regen- eration requires an effective technique for obtaining purified Schwann cells. In vivo and in vitro pre-degeneration of peripheral nerves have been shown to obtain high-purity Schwann cells. We believed that in vitro pre-degeneration was simple and controllable, and available for the clinic. Thus, we co-cultured the crushed sciatic nerves with bone marrow-derived cells in vitro. Results demonstrated that, 3 hours after injury, a large number of mononuclear cells moved to the crushed nerves and a large number of bone marrow-derived cells infiltrated the nerve segments. These changes promoted the degradation of the nerve segments, and the dedifferentiation and proliferation of Schwann cells. Neural cell adhesion molecule and glial fibrillary acidic protein expression were detected in the crushed nerves. Schwann cell yield was 9.08 ± 2.01 ×104/mg. The purity of primary cultured Schwann cells was 88.4 ± 5.79%. These indicate a successful new method for ob- taining Schwann cells of high purity and yield from adult crushed sciatic nerve using bone marrow-derived cells.

关键词： nerve regeneration bone marrow-derived cells Schwatm cells co-culture in vitro pre-degeneration ded!fferentiation glial fibrillaryacidic protein neural cell adhesion molecule mononuclear cells neural regeneration

An efficient synthesis of novel pyridothieno-fused thiazolo[3,2-α]pyrimidinones via Pictet–Spengler reaction

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Chinese Chemical Letters 2016年第6期27卷 953-956页

作者： Dao-Lin Wang Dong Wang Liang yan Guang-Yu Pan jian-Nan yang College of Chemistry and Chemical Engineering Liaoning Key Laboratory of Synthesis and Application of Functional Compound Bohai University Jinzhou 121003 China

An efficient method for the synthesis of novel pyrido[3＇＇,2＇＇：4＇,5＇]thieno[3＇,2＇：2,3]pyrido [4,5：d][1,3]thiazolo[3,2-a]pyrimidine-4-one derivatives（5） has been developed using a Pictet–Spengler reaction between 2-（3-aminothieno[2,3-b]pyridin-2-yl）thiazolo[3,2-a] pyrimidin-5-one（3）, which could be obtained from the condensation of 7-（chloromethyl）-5H-thiazolo[3,2-a]pyrimidin-5-one（1） with3-cyanopyridine-2-thione（2） via Thorpe–Ziegler isomerization, and aromatic aldehydes under NH2SO3 H as catalysis in good yields.

关键词： 7-Chloromethyl-5H-thiazolo[3 2-α]pyrimidin-5-one 3-Cyanopyridine-2-thione Pyrido[3'' 2'' 4' 5']thieno[3' 2' 2 3]pyrido [4 5:d] [1 3]Thiazolo[3 2-a]pyrimidine-4 one Thorpe–Ziegler reaction Pictet–Spengler reaction

Fascin promotes the motility and invasiveness of pancreatic cancer cells

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World Journal of Gastroenterology 2011年第40期17卷 4470-4478页

作者： yan-Feng Xu Shuang-Ni Yu Zhao-Hui Lu jian-Ping Liu Jie Chen Department of Pathology Peking Union Medical College Hospital Chinese Academy of Medical Sciences and Peking Union Medical College Tsinghua University Beijing 100730 China

AIM:To explore the role of actin-bundling protein, fascin during the progression of pancreatic cancer. METHODS:The plasmid expressing human fascin-1 was stably transfected into the pancreatic cancer cell line MIA PaCa-2. The proliferation, cell cycle, motility, scattering, invasiveness and organization of the actin filament system in fascin-transfected MIA PaCa-2 cells and control non-transfected cells were determined. RESULTS:Heterogeneous overexpression of fascin markedly enhanced the motility, scattering, and inva-siveness of MIA PaCa-2 cells. However, overexpression of fascin had minimal effect on MIA PaCa-2 cell pro-liferation and cell cycle. In addition, cell morphology and organization of the actin filament system were distinctly altered in fascin overexpressed cells. When transplanted into BALB/c-nu mice, fascin-transfected pancreatic cancer cells developed solid tumors at a slightly slower rate, but these tumors displayed more aggressive behavior in comparison with control tumors. CONCLUSION: Fascin promotes pancreatic cancer cell migration, invasion and scattering, thus contributes to the aggressive behavior of pancreatic cancer cells.

关键词： Fascin Invasiveness Motility Pancreaticcancer

New In-Situ LA-ICP-MS U-Pb Ages of Uraninite from the Mianhuakeng Uranium Deposit, Northern Guangdong Province, China: Constraint on the Metallogenic Mechanism

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Acta Geologica Sinica(English Edition) 2018年第2期92卷 852-854页

作者： zhong Fujun PAN Jiayong QI Jiaming yan Jie LIU Wenquan LI Haidong State Key Laboratory Breeding Base of Nuclear and Environment East China University of Technology Research Institute No.290 CNNC

Objective Hydrothermal uranium deposits in South China have become one of the main uranium producers over the past several decades. Numerous uranium deposits are densely distributed in the Guidong and Zhuguangshan plutons of the Nanling region, which constitute the largest uranium mineralization area in China （Cai Yuqi et al., 2015）. The Mianhuakeng （MHK） uranium deposit, one of the largest granite-type uranium deposits in South China, is located in the central-southern part of the Zhuguangshan pluton, northern Guangdong Province, and is characterized by high grade ores （U： 0.01%-1.2%）, large reserves （〉10000 t） and a large vertical extent of mineralization （ca. 1000 m）.

关键词： Pb China MHK ICP MS

Carbon sequestration of plantation in Beijing-Tianjin sand source areas

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Journal of Mountain Science 2018年第10期15卷 2148-2158页

作者： LIU Xiu-ping ZHANG Wan-jun CAO jian-sheng yanG Bai CAI yan-jiang Key Laboratory of Agricultural Water Resources Hebei Key Laboratory of Agricultural Water-Saving Center for Agricultural Resources Research Institute of Genetics and Developmental Biology Chinese Academy of Sciences Environmental Sciences Division Oak Ridge National Laboratory Key Laboratory of Mountain Environment Evolvement and Regulation Institute of Mountain Hazards and Environment Chinese Academy of Sciences

The Beijing-Tianjin Sand Source Control Project(BTSSCP), a national ecological restoration project, was launched to construct an ecological protection system in the Beijing-Tianjin sand source areas to reduce dust hazards. The carbon sequestration dynamics can be used to assess the ecological effects of an ecological restoration project. Here, we conducted vegetation and soil study to assess the carbon sequestration in the plantations with 10 years old stands in Beijing-Tianjin sand source areas. The results at the site scales indicated that the average net increase of plantation ecosystem carbon stock was 33.8 Mg C ha^(-1), with an annual increase rate of 3.38 Mg C ha^(-1) yr^(-1). The average net increase of carbon varied among regions, vegetation types, and forest management activities. Soil bulk density in the top soil decreased slightly after 10-year implementation of the project. Coniferous forests and shrubs are suitable plant species for sand source *** restoration in the plantations is a practical and feasible and promising approach for enhancing ecosystem carbon sequestration potential.

关键词： Afforestation Carbon sequestration Carbon density Forest management Restoration Sand source control

Release test of alliin/alliinase double-layer tablet by HPLC-Allicin determination

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Journal of Pharmaceutical Analysis 2013年第3期3卷 187-192页

作者： yan Liang Jing-Jing Zhang Qi-Bing Zhang zhong-Xia Wang Zong-Ning Yin Xin-Xia Li jian Chen Li-Ming Ye West China School of Pharmacy Sichuan University Xinjiang Medical University

A simple, precise and accurate method was developed and validated for the determination of allicin release from alliin/alliinase double-layer tablets. According to Appendix XC Ⅱ of Chinese Pharmacopoeia 2010 edition Volume II, a small glass-method was adopted at the rotational speed of 100 r/min using 100 mL phosphate buffer （pH 6.8） as release medium. The release amount was determined by HPLC with a C18 column （250 mm × 4.6 mm, 5 μm） using the mobile phase consisting of methanol -0.4% carboxylic acid （65：35） at a flow rate of 1 mL/min and UV detection at 242 nm. The current method demonstrates good linearity over the range 4.052- 405.2 μg/mL （r2=0.9999） with an average recovery of 105.5%（RSD= 1.25%）. The accumulative release of alliin/alliinase double-layer tablets had good homogeneity for withinand betweenbatches. The method established is simple, accurate and repeatable for the determination of allicin release from alliin/alliinase double-layer tablets.

关键词： Alliin/alliinase Double-layer tablet Allicin Release test HPLC

Accumulation and distribution of arsenic and cadmium by tea plants

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2008年第3期9卷 265-270页

作者： Yuan-zhi SHI jian-yun RUAN Li-feng MA Wen-yan HAN Fang WANG Tea Research Institute Chinese Academy of Agricultural Sciences Hangzhou 310008 China

It is important to research the rules about accumulation and distribution of arsenic and cadmium by tea plants,which will give us some scientific ideas about how to control the contents of arsenic and cadmium in *** this study,by field investigation and pot trial,we found that mobility of arsenic and cadmium in tea plants was *** arsenic and cadmium absorbed were fixed in feeding roots and only small amount was transported to the above-ground *** of arsenic and cadmium,based on their concentrations of unit dry matter,in tea plants grown on un-contaminated soil was in the order:feeding roots>stems≈main roots>old leaves>young *** tea plants were grown on polluted soils simulated by adding salts of these two metals,feeding roots possibly acted as a buffer and defense,and arsenic and cadmium were transported less to the aboveground *** concentration of cadmium in soil significantly and negatively correlated with chlorophyll content,photosynthetic rate,transpiration rate and biomass production of tea plants.

关键词： Tea plant Arsenic (As) Cadmium (Cd) Absorption Accumulation

重症肺炎患者血清IL-17及IL-12P70与CD64表达水平及其与预后的关系

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中华医院感染学杂志 2024年第16期34卷 2452-2456页

作者：蔡剑英陶学芳王国文赵梁燕孙永珍绍兴文理学院附属医院呼吸与危重症医学科浙江绍兴312000

目的分析重症肺炎患者血清白细胞介素(IL)-17、IL-12P70、簇分化抗原64(CD64)水平与病情严重程度及预后的关系。方法选取2021年1月-2023年6月绍兴文理学院附属医院收治的重症肺炎患者120例作为重症组,同期选取普通肺炎患者60例作为普通... 详细信息

目的分析重症肺炎患者血清白细胞介素(IL)-17、IL-12P70、簇分化抗原64(CD64)水平与病情严重程度及预后的关系。方法选取2021年1月-2023年6月绍兴文理学院附属医院收治的重症肺炎患者120例作为重症组,同期选取普通肺炎患者60例作为普通组;分析重症肺炎患者病原菌,采集患者入院24h内静脉血5ml,检测血清IL-17、IL-12P70、CD64,分析其与急性生理慢性健康评分(APACHEⅡ)的相关性;根据28d疾病转归情况,比较不同预后患者血清IL-17、IL-12P70、CD64水平;受试者操作特征(ROC)曲线评估IL-17、IL-12P70、CD64对患者预后价值。结果120例重症肺炎患者共分离病原菌138株,以革兰阴性菌为主;重症组血清IL-17、IL-12P70、CD64水平高于普通组(P<0.05);血清IL-17、IL-12P70、CD64水平与APACHEⅡ评分均呈正相关(r=0.437,0.367,0.482P<0.05);120例重症肺炎患者28d内死亡36例,存活84例,存活组患者血清IL-17、IL-12P70、CD64水平低于死亡组(P<0.05);ROC曲线分析显示,血清IL-17、IL-12P70、CD64及联合检测评估患者预后的曲线下面积(AUC)分别为0.722、0.681、0.680和0.843。结论血清IL-17、IL-12P70、CD64在重症肺炎患者中明显升高,三者联合检测具有评估患者预后的价值。

关键词：重症肺炎白细胞介素-17 簇分化抗原64 病情严重程度预后

A Real-time Monitoring and Early Warning System for Landslides in Southwest China

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Journal of Mountain Science 2015年第5期12卷 1219-1228页

作者： JU Neng-pan HUANG jian HUANG Run-qiu HE Chao-yang LI yan-rong State Key Laboratory of Geohazard Prevention and Geoenvironment Protection Chengdu University of Technology AGECON Ltd.

Landslides not only cause property losses,but also kill and injure large numbers of people every year in the mountainous areas. These losses and casualties may be avoided to some extent by early warning systems for landslides. In this paper, a realtime monitoring network and a computer-aided automatic early warning system(EWS) are presented with details of their design and an example of application in the Longjingwan landslide, Kaiyang County, Guizhou Province. Then, according to principle simple method of landslide prediction, the setting of alarm levels and the design of appropriate counter-measures are presented. A four-level early warning system(Zero, Outlook, Attention and Warning) has been adopted, and the velocity threshold was selected as the main warning threshold for the landslide occurrence, but expert judgment is included in the EWS to avoid false alarms. A case study shows the applicability and reliability for landslide risk management, and recommendations are presented for other similar projects.

关键词： Landslide Early warning system (EWS)Wireless sensor network Velocity threshold Longjingwan landslide

The Arabidopsis CROWDED NUCLEI genes regulate seed germination by modulating degradation of ABI5 protein

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Journal of Integrative Plant Biology 2016年第7期58卷 669-678页

作者： Wenming Zhao Chunmei Guan jian Feng yan Liang Ni Zhan jianru Zuo Bo Ren State Key Laboratory of Plant Genomics and National Plant Gene Research Center Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049china Institute of Microbiology Chinese Academy of SciencesBeijing 100101China John Innes Centre Norwich NR4 7UHUnited Kingdom Institute of Biotechnology Zhejiang UniversityHangzhou 310058China

In Arabidopsis, the phytohormone abscisic acid （ABA） plays a vital role in inhibiting seed germination and in postgermination seedling establishment. In the ABA signaling pathway, ABI5, a basic Leu zipper transcription factor, has important functions in the regulation of seed germination. ABI5 protein localizes in nuclear bodies, along with AFP, COP1, and SIZ1, and was degraded through the 26S proteasome pathway. However, the mechanisms of ABI5 nuclear body formation and ABI5 protein degradation remain obscure. In this study, we found that the Arabidopsis CROWDED NUCLEI （CRWN） proteins, predicted nuclear matrix proteins essential for maintenance of nuclear morphology, also participate in ABA-controlled seed germination by regulating the degradation of ABI5 protein. During seed germination, the crwn mutants are hypersensitive to ABA and have higher levels of ABI5 protein compared to wild type. Genetic analysis suggested that CRWNs act upstream of ABIS. The observation that CRWN3 colocalizes with ABI5 in nuclear bodies indicates that CRWNs might participate in ABI5 protein degrada- tion in nuclear bodies. Moreover, we revealed that the extreme C-terminal of CRWN3 protein is necessary for its function in the response to ABA in germination. Our results suggested important roles of CRWNs in ABI5 nuclear body organization and ABI5 protein degradation during seed germination.

关键词： ABI5 Arabidopsis CROWDED NUCLEI germination nuclear body