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The environmental analysis and site selection of mussel and large yellow croaker aquaculture areas based on high resolution remote sensing

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Acta Oceanologica Sinica 2024年第3期43卷 66-86页

作者： Lina Cai Jie Yin xiaojun yan Yongdong Zhou Rong Tang Menghan Yu Marine Science and Technology College Zhejiang Ocean UniversityZhoushan 316022China Zhejiang Marine Fisheries Research Institute Zhejiang Ocean UniversityZhoushan 316004China

Mussel aquaculture and large yellow croaker aquaculture areas and their environmental characteristics in Zhoushan were analyzed using satellite data and in-situ surveys.A new two-step remote sensing method was proposed and applied to determine the basic environmental characteristics of the best mussel and large yellow croaker aquaculture *** methodology includes the first step of extraction of the location distribution and the second step of the extraction of internal environmental *** fishery ranching index(FRI1,FRI2)was established to extract the mussel and the large yellow croaker aquaculture area in Zhoushan,using Gaofen-1(GF-1)and Gaofen-6(GF-6)satellite data with a special resolution of 2 *** the second step,the environmental factors such as sea surface temperature(SST),chlorophyll a(Chl-a)concentration,current and tide,suspended sediment concentration(SSC)in mussel aquaculture area and large yellow croaker aquaculture area were extracted and analyzed in *** results show the following three points.(1)For the extraction of the mussel aquaculture area,FRI1 and FRI2 are complementary,and the combination of FRI1 and FRI2 is suitable to extract the mussel aquaculture *** for the large yellow croaker aquaculture area extraction,FRI2 is suitable.(2)Mussel aquaculture and the large yellow croaker aquaculture area in Zhoushan are mainly located on the side near the islands that are away from the eastern open *** water environment factor template suitable for mussel and large yellow croaker aquaculture was determined.(3)This two-step remote sensing method can be used for the preliminary screening of potential site selection for the mussels and large yellow croaker aquaculture area in the *** fishery ranching index(FRI1,FRI2)in this paper can be applied to extract the mussel and large yellow croaker aquaculture areas in coastal waters around the world.

关键词： mussel aquaculture area large yellow croaker aquaculture area high resolution satellite site selection environmental analysis

Analysis and Characterization of the GABA Transaminase and Succinate Semialdehyde Dehydrogenase Genes in the Microalga Isochrysis zhanjiangensis in Response to Abiotic Stresses

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Journal of Ocean University of China 2024年第3期23卷 775-785页

作者： TIAN Jiaojiao ZHANG Lin LU Xinyue YE Lingzhi WU Yuanyuan CHEN Deshui CAO Jiayi JIANG Jiaxin XU Jilin yan xiaojun Key Laboratory of Marine Biotechnology of Zhejiang Province School of Marine SciencesNingbo UniversityNingbo 315211China Key Laboratory of Aquacultural Biotechnology Ministry of Education Ningbo UniversityNingbo 315211China Laizhou Bay Marine Technology Co. Ltd.Yantai 261400China Fujian Dalai Seed Science and Technology Co. Ltd.Ningde 352101China

Gamma-aminobutyric acid(GABA),widely existing in different organisms,is rapidly accumulated in plants in response to environmental *** main biosynthesis and degradation pathways of GABA constitute the GABA shunt,which is tied to the tricarboxylic acid(TCA)*** transaminase(GABA-T)and succinate semialdehyde dehydrogenase(SSADH)are two essential enzymes for the GABA degradation *** there are abundant studies on GABA shunt in higher plants at the physiological and genetic levels,research on its role in microalgae remains *** study aimed at exploring the function of GABA-T and SSADH genes in Isochrysis zhanjiangensis,an important diet microalga,under different *** cloned two GABA-T genes,IzGABA-T1 and IzGABA-T2,and one SSADH gene IzSSADH from Isochrysis zhanjiangensis and conducted heterologous expression *** results showed that the overexpression of IzGABA-T1 or IzGABA-T2 enhanced the survival rates of yeast transformants under heat or NaCl stress,while the overexpression of IzSSADH improved yeast tolerance to NaCl stress but had no obvious effect on heat ***,the results of quantitative real-time polymerase chain reaction(qPCR)showed that IzGABA-T1 transcription increased in the HT(salinity 25,35℃)and LS(salinity 15,25℃)*** 24 h,the IzGABA-T2 transcriptions increased in the HT,LS,and HS(salinity 35,25℃)groups,but their transcription levels decreased in all groups at 48 *** transcription increased in the LS *** results suggest that IzGABA-T1,IzGABA-T2,and IzSSADH are associated with temperature and salinity stresses and possess a certain preference for different stresses.

关键词： abiotic stress GABA heterologous expression Isochrysis zhanjiangensis transcription

Liver mitochondrial and whole-animal level metabolic compensation in a catfish during seasonal acclimatization

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Current Zoology 2011年第1期57卷 109-115页

作者： Yulian yan xiaojun XIE Institute of Hydrobiology and Water Environment School of Life Science Southwest University Chongqing 400715 China

To examine whether metabolic compensation during seasonal acclimatization at the liver mitochondrial level is consistent with that at the whole-animal level, respiration rates of liver mitochondria and resting metabolic rates in winter- and sum- meracclimatized southern catfish （Silurus meridionalis Chen） were measured. At 12.5, 17.5, 22.5, 27.5 and 32.5~C, the mean values of state 3 respiration rates were 12.21, 13.84, 18.96, 24.78 and 32.01 nmol O2min-1 mg-1 mitochondrial protein in the winter group, and 8.56, 9.20, 17.32, 22.74 and 26.32 nmol 02 min-1 mgq in the summer group, respectively. At the five assay temperatures the resting metabolic rates were 24.86, 42.68, 61.59, 84.10 and 125.65 mg 02 h-1 kgI body mass in the winter group, and 22.89, 40.59, 52.94, 75.13 and 109.35 mg Oz h-1 kg-1 in the summer group, respectively. Total mitochondrial respiration rates in the liver organ were estimated based on state 3 respiration rates, mitochondrial protein content and organ mass, and the mean values were 72.96, 71.87, 112.47, 167.35 and 183.27 nmol Ozmin-lin the winter group, and were 47.89, 47.39, 105.67, 138.18 and 132.29 nmol 02 min-1 in the summer group, respectively. Metabolic compensation caused by seasonal acclimatization occurred at the liver mitochondrial level and compensation at the liver organ level was found to be more efficient because of an in- crease in metabolic capacity of mitochondria and a boost in organ mass. Metabolic compensation at the whole-animal level was not detected. During seasonal acclimatization, the effect of metabolic compensation at liver mitochondrial level is inconsistent with that at the whole-animal level in the southern catfish. This may be due to different degrees of regulation of metabolic mechanisms among various tissues and organs in an acclimatized organism

关键词： Metabolic compensation Mitochondrial respiration Seasonal acclimatization Silurus meridionalis

长期饥饿胁迫下厚壳贻贝的生理生化响应及性腺转录组

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水产学报 2024年第4期48卷 268-279页

作者：孙闻婧陈传悦梁泽玮廖智严小军张晓林浙江海洋大学海洋科学与技术学院宁波大学海洋学院

为探究贻贝在饥饿胁迫下的生理生化响应及分子适应机制，本研究以2龄厚壳贻贝为对象，开展了正常投喂(对照组)和饥饿处理(饥饿组)条件下的存活率统计、能量代谢相关酶活性测定以及性腺转录组测序及分析。结果显示，厚壳贻贝在90 d的饥... 详细信息

为探究贻贝在饥饿胁迫下的生理生化响应及分子适应机制，本研究以2龄厚壳贻贝为对象，开展了正常投喂(对照组)和饥饿处理(饥饿组)条件下的存活率统计、能量代谢相关酶活性测定以及性腺转录组测序及分析。结果显示，厚壳贻贝在90 d的饥饿处理下存活率高达86%。饥饿胁迫下贻贝性腺中磷酸烯醇式丙酮酸羧激酶(PEPCK)活性显著增加，己糖激酶(HK)、磷酸果糖激酶(PFK)、琥珀酸脱氢酶(SDH)、苹果酸脱氢酶(MDH)活性均显著降低。通过Illumina Hiseq×10高通量测序平台对对照组和饥饿组厚壳贻贝性腺组织进行转录组测序，分别获得31 596 762和26 810 506个在其基因组上具有唯一注释的有效数据，并筛选出4 130个差异表达基因，包括2 082个上调和2 048个下调表达基因。GO功能分析结果显示，差异基因主要在富集在代谢过程、细胞器组织以及酶活性等功能。KEGG富集分析结果表明，差异基因显著富集在DNA复制、错配修复、碱基切除修复等与细胞分裂有关的代谢通路上。研究表明，贻贝在饥饿下通过降低细胞的能量代谢以及减缓细胞分裂等生理活动来维持饥饿胁迫下的生存。本研究初步揭示了厚壳贻贝在饥饿胁迫下的生理生化响应和分子调控机制，为今后深入解析贻贝应对饥饿胁迫的分子策略提供重要的理论依据，同时为揭示其适应饥饿胁迫的能量利用和再分配以及生理对策提供新的思路。

关键词：厚壳贻贝长期饥饿存活率能量代谢酶活性腺转录组

A New Kind of Pilot Controlled Proportional Direction Valve with Internal Flow Feedback

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Chinese Journal of Mechanical Engineering 2010年第1期23卷 60-65页

作者： QUAN Long XU Xiaoqing yan Zheng ZHANG xiaojun Institute of Mechatronics Engineering Taiyuan University of Technology Taiyuan 030024 China

Proportional direction valve is one of the most fundamental elements in electronic-hydraulic control technique. Its function is to control the operating speed, direction, position, and strength of output force of the hydraulic actuator continuously. Considering the different application and the cost, the existing technique mainly includes the internal feedback valve used in open loop system, and the electronic closed loop controlled valve used in closed loop system. Because of their different mechanical structure and the gre at different in performance, it brings inconvenience for customer to select, also inconvenience for enterprise to produce. Aiming at this problem, the idea of combining the above two kinds of valves into one body is proposed first, and then the new valve＇s structure to realize this target is designed. The idea intends to apply the displacement pilot flow feedback control principle in present 2-position 2-way valve system to the proportional direction valve of 3-position 4-way system. Newly designed feed forward controller can decouple the interference between the internal feedback and the electronic closed loop. Redundant conversion is designed to electronic switch mode. Experiment on dynamic and static characteristic of new proportional direction valve in internal feedback control mode and electronic closed loop control mode is discussed to prove the new theory is correct. Although the new valve is of excellent dynamic response characteristic, its steady control characteristic in open loop control mode needs to be improved further. The research results prepare one new fundamental element for electronic-hydraulic control technology.

关键词： pilot flow feedback proportional direction valve working principle characteristic research

葛根芩连汤及其加减方改善2型糖尿病胰岛素抵抗作用研究进展

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中国实验方剂学杂志 2024年第15期30卷 256-263页

作者：刘艺敏吉燕华陈梦婕严小军刘红宁曾治君江西中医药大学中医基础理论分化发展研究中心江西省中医病因生物学重点实验室南昌330004

葛根芩连汤出自《伤寒杂病论》,为表里双解治疗协热下利的代表方剂,全方由葛根、黄芩、黄连、炙甘草配伍而成,四药合用可外疏内清,共达清热止利,表里同解之效。现临床上基于异病同治的原则将葛根芩连汤用于2型糖尿病(T2DM)的治疗,取得... 详细信息

葛根芩连汤出自《伤寒杂病论》,为表里双解治疗协热下利的代表方剂,全方由葛根、黄芩、黄连、炙甘草配伍而成,四药合用可外疏内清,共达清热止利,表里同解之效。现临床上基于异病同治的原则将葛根芩连汤用于2型糖尿病(T2DM)的治疗,取得了良好的疗效。T2DM是一种以血糖水平升高为主要特征的慢性代谢性疾病,其病因和发病机制十分复杂,主要与遗传、生活方式、环境及饮食等因素相关。临床观察及实验研究显示,葛根芩连汤及其药效成分可有效防治T2DM。临床上常将葛根芩连汤加减应用或联合西药应用,其治疗效果明显优于单独使用西药。临床实践证实葛根芩连汤可以有效缓解T2DM患者临床症状,减轻胃肠道等不良反应,并减少并发症的发生。实验研究表明葛根芩连汤可通过调控胰岛传导通路及炎症信号通路、缓解氧化应激反应、调节肠道菌群等方式来增强患者胰岛功能,改善胰岛素抵抗,从而治疗T2DM。但目前关于葛根芩连汤通过改善胰岛素抵抗治疗T2DM的机制尚待进一步研究,故该文就葛根芩连汤改善T2DM胰岛素抵抗的临床及实验基础研究进展进行系统综述,以期为葛根芩连汤治疗T2DM作用机制的后续深入研究及其临床防治提供理论和数据参考。

关键词：葛根芩连汤 2型糖尿病胰岛素抵抗临床应用作用机制

Passive attitude stabilization of ionic-wind-powered micro air vehicles

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Chinese Journal of Aeronautics 2023年第7期36卷 412-419页

作者： Hengyu ZHANG Jiaming LENG Zhiwei LIU Mingjing QI xiaojun yan School of Energy and Power Engineering Beihang UniversityBeijing 100191China

The ionic-wind-powered Micro Air Vehicles(MAVs)can achieve a higher thrust-toweight ratio than other ***,this kind of MAV has not yet achieved controlled flight because of the unstable thrust produced by the ionic wind and the dynamic instability related to the small *** this paper,a passive attitude stabilization method of the ionic-wind-powered MAV using air dampers is *** key factors that influence the performance of the air dampers,including the layout,position,and area of the air dampers,are theoretically *** appropriate optimal position of the air dampers is also obtained by Monte Carlo stochastic *** the proposed passive attitude stabilization method is applied to the ionic-wind-powered MAVs of different wingspan(2 cm and 6.3 cm).Finally,the experimental results show that using the proposed method,attitude stabilization is achieved for the first time for the ionic-wind-powered ***,the altitude control of an ionic-wind-powered MAV with a wingspan of 6.3 cm is also demonstrated.

关键词： Air dampers Altitude control Ionic wind propulsion Micro air vehicle Monte Carlo methods Stability

大黄鱼PPAR基因家族特征及其在饥饿和温度胁迫中的功能分析

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浙江海洋大学学报（自然科学版） 2024年第2期43卷 111-118页

作者：赵晴江丽华严小军浙江海洋大学国家海洋设施养殖工程技术研究中心浙江舟山316022

分析了大黄鱼PPAR基因家族的序列结构及其在饥饿和温度胁迫下的表达特征。结果显示,除PPARg同时具有PPARg_N和PPARg_N_superfamily这2种结构域外,大黄鱼PPAR基因家族在结构上基本一致。此外,PPAR基因家族基因存在组织特异性表达,在饥饿... 详细信息

分析了大黄鱼PPAR基因家族的序列结构及其在饥饿和温度胁迫下的表达特征。结果显示,除PPARg同时具有PPARg_N和PPARg_N_superfamily这2种结构域外,大黄鱼PPAR基因家族在结构上基本一致。此外,PPAR基因家族基因存在组织特异性表达,在饥饿胁迫下,随着饥饿胁迫时间推移,PPAR基因家族的表达量皆呈增长趋势,其中,PPARg在肌肉中表达量最高,推测PPAR基因家族在大黄鱼脂质代谢中发挥了作用。在温度胁迫下,PPAR基因家族在肝脏中的表达都呈波动变化趋势,而PPARg的表达量则随着胁迫时间的延长而显著上升。该研究为了解PPAR基因家族在调节大黄鱼脂质代谢中的作用提供了基础资料。

关键词：大黄鱼 PPAR基因家族饥饿胁迫温度胁迫

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关于提振香港地区发展新动能的若干思考

港澳研究 2024年第2期 39-48,94,95页

作者：阎小骏郝咏李拉全国港澳研究会香港大学中国制度研究中心中国香港香港大学政治与公共行政学系中国香港

如何全面、有效提振“兴”的动能,实现香港地区经济高质量发展,是当前各界共同关注的重大议题。我们认为,面对全球经济环境的快速变化和科技创新的迅猛发展,香港地区宜主动激发智力、资金、管理三方面优势,以整合性、全局性、前瞻性、... 详细信息

如何全面、有效提振“兴”的动能,实现香港地区经济高质量发展,是当前各界共同关注的重大议题。我们认为,面对全球经济环境的快速变化和科技创新的迅猛发展,香港地区宜主动激发智力、资金、管理三方面优势,以整合性、全局性、前瞻性、普惠性为原则,以战略性支柱产业经济带和创新型未来产业经济带建设为抓手,以稳固的产业人才培养体系、灵活的金融体系,以及健全的法律法规和知识产权保护体系为保障,积极构建国际金融、国际算力、中间试验、技术交易及创业服务五大产业集群,以有效激发以新质生产力为基石的新的发展动能。这一战略布局旨在平衡短期与长期财政收入,确保香港地区在全球经济中的竞争力和影响力,为实现香港地区长期繁荣稳定奠定坚实基础。

关键词：新质生产力财政转型产业政策