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Loss of heterozygosity on long arm of chromosome 22 in sporadic colorectal carcinoma

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World Journal of Gastroenterology 2002年第4期8卷 668-673页

作者： Chong-Zhi Zhou Zhi-Hai peng Fang Zhang Guo-Qiang Qiu Department of General Surgery,Shanghai First People Hospital,Shanghai 200080,China Lin He Shanghai Institutes for Biological Science,Chinese Academy of Science,Shanghai 200031,China Department of General Surgery Shanghai First People Hospital 85 Wujin Road Shanghai 200080 China.

AIM: The loss of heterozygosity (loh) on tumorsuppressor genes is believed to play a key role incarcinogenesis of colorectal cancer, In this study, weanalyzed the loh at 5 loci on the long arm ofchromosome 22 in sporadic colorectal cancer to identifyadditional loci involved in colorectal ***: Five polymorphic microsatellite markerswere analyzed in 83 cases of colorectal and normal DNAby PCR. PCR products were eletrophoresed on an ABI377 DNA sequencer; Genescan 3.1 and Genotype 2.1software were used for loh scanning and analysisComparison between loh frequency andclinicopathological data were performed by x2 test. P＜0.05 was considered as statistically ***: The average loh frequency on chromosome22q was 28.38 %. The region between markersD22S280 and D22S274 (22q12.2-q13.33) exhibitedrelatively high loh frequency. The two highest loh lociwith frequencies of 35.09 % and 34.04 % wasidentified on D22S280 (22q12.2-12.3) and D22S274(22q13.32-13.33). 8 cases showed loh at allinformative loci, suggesting that one chromosome 22qhad been completely lost. On D22S274 locus, lohfrequency of rectal cancer was 50 % (9/18), which washigher than that of proximal colon cancer (12 %, 2/17) (P=0.018). The frequency of distal colon cancer was42 % (5/12), also higher than that of proximal coloncancer. But there was no statistical significance. Puttingboth the tumors in distal colon and rectum togetherinto consideration, the frequency, 47 % (14/30), washigher than that of proximal colon cancer (P=0.015),suggesting the mechanism of carcinogenisis wasdifferent in both ***: This study provided evidence for theinvolvement of putative tumor suppressor genes relatedto the sporadic colorectal carcinoma on chromosome22q. The tumor-suppressor-gene(s) might locate on the22q12.2-12.3 and/or 22q13.32-13.33.

关键词：散发性直肠癌 22染色体等位基因杂合性丢失

口服十一酸睾酮胶丸联合麒麟丸治疗男性迟发性性腺功能减退症的临床观察

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中华男科学杂志 2017年第5期23卷 455-458页

作者：毛俊彪陈小刚桂定文彭伟张杰鄂东医疗集团黄石市中心医院(湖北理工学院附属医院)泌尿外科湖北黄石435000 肾脏疾病发生与干预湖北省重点实验室湖北黄石435000

目的:观察口服十一酸睾酮胶丸联合麒麟丸治疗男性迟发性性腺功能减退症(loh)患者的临床疗效。方法:符合纳入标准的63例loh患者随机分为对照组和试验组,对照组口服十一酸睾酮胶丸,试验组口服十一酸睾酮胶丸和麒麟丸。十一酸睾酮胶丸用法:... 详细信息

目的:观察口服十一酸睾酮胶丸联合麒麟丸治疗男性迟发性性腺功能减退症(loh)患者的临床疗效。方法:符合纳入标准的63例loh患者随机分为对照组和试验组,对照组口服十一酸睾酮胶丸,试验组口服十一酸睾酮胶丸和麒麟丸。十一酸睾酮胶丸用法:80 mg/次,1次/d,连续服用3个月;麒麟丸用法:6 g/次,3次/d,连续服用3个月。比较组内和组间治疗前后的IIEF-5评分、老年男性症状问卷(AMS)评分及血清总睾酮(TT)水平的变化。结果:两组间各项数据无明显差异(P>0.05)。但治疗后试验组IIEF-5评分(21.7±5.8)分和TT值(16.7±2.2)nmol/L均显著高于对照组(15.9±4.7)分和(13.1±2.8)nmol/L(Ploh的疗效比单用十一酸睾酮胶丸治疗效果更显著,且不增加不良反应发生率,有一定的临床应用前景。

关键词：麒麟丸十一酸睾酮胶丸迟发性性腺功能减退症

Mutation and methylation of hMLH1 in gastric carcinomas with microsatellite instability

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World Journal of Gastroenterology 2003年第4期9卷 655-659页

作者： Dian-Chun Fang Rong-Quan Wang Shi-Ming yang Jian-Ming yang Hai-Feng Liu Gui-yong peng Tian-Li Xiao yuan-Hui Luo Department of Gastroenterology,Southwest Hospital,Third Military Medical University,Chongqing,400038,China Southwest Hospital Third Military Medical University Chongqing 400038 China. fangdianchun@***

AIM: To appraise the correlation of mutation and methylation of hMSH1 with microsatellite instability (MSI) in gastric ***: Mutation of hMLH1 was detected by Twodimensional electrophoresis (Two-D) and DNA sequencing;Methylation of hMLH1 promoter was measured with methylation-specific PCR; MST was analyzed by PCR-basecl ***: Sixty-eight cases of sporadic gastric carcinoma were studied for mutation and methylation of hMLH1 promoter and MSI. Three mutations were found, two of them were caused by a single bp substitution and one was caused by a 2 bp substitution, which displayed similar Two-D band *** of hMLH1 promoter was detected in 11(16.2%)gastric cancer. By using five MSI markers, MSI in at least one locus was detected in 17/68(25 %) of the tumors *** hMLH1 mutations were all detected in MSI-H (≥2 loci,n=8), but no mutation was found in MSI-L (only one locus,n=9) or MSS (tumor lacking MSI or stable, n=51). Methylation frequency of hMLH1 in MSI-H (87.5 %, 7/8) was significantly higher than that in MSI-L (11.1%, 1/9) or MSS (5.9%, 3/51)(P＜0.01-0.001), but no difference was found between MSI-L and MSS (P＞0.05).CONCLUSION: Both mutation and methylation of hMLH1are involved in the MSI pathway but not related to the loh pathway in gastric carcinogenesis.

关键词： hMLH1基因甲基化胃癌微卫星不稳定性聚合酶链反应 DNA损伤

Mechanisms inactivating the gene for E-cadherin in sporadic gastric carcinomas

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World Journal of Gastroenterology 2006年第14期12卷 2168-2173页

作者： yao-Chi Liu Chen-yang Shen Hurng-Sheng Wu Tsai-yuan Hsieh De-Chuan Chan Cheng-Jueng Chen Jyh-Cherng yu Cheng-Ping yu Horng-Jyh Harn peng-Jen Chen Chung-Bao Hsieh Teng-Wei Chen Huan-Mieng Hsu Division of General Surgery Department of Surgery Tri-Service General Hospital National Defense Medical Center Taipei Taiwan China Division of Gastroenterology Department of Internal Medicine Tri-Service General Hospital National Defense Medical Center Taipei Taiwan China Department of Pathology Tri-Scrvice General Hospital National Defense Medical Center Taipei Taiwan China Department of Pathology Buddhist Tzu-Chi General Hospital Hualien Taiwan China Department of Surgery Show Chwan Memorial Hospital Changhua Taiwan China Institute of Biomedical Sciences Academia Sinica Taipei Taiwan China

瞄准：为了学习 CDH1/E-cadherin (电子恶棍) 的角色，基因改变介绍在胃的癌(GC ) 在激活在 CDH1 的机制包括变化，杂合现象(loh ) 的损失，倡导者多型性和 hypermethylation。方法：标本与 GC 从 70 个病人通过手术被收集。Allelotypin... 详细信息

瞄准：为了学习 CDH1/E-cadherin (电子恶棍) 的角色，基因改变介绍在胃的癌(GC ) 在激活在 CDH1 的机制包括变化，杂合现象(loh ) 的损失，倡导者多型性和 hypermethylation。方法：标本与 GC 从 70 个病人通过手术被收集。Allelotyping PCR 和定序的 loh，使中毒的高压力液相色谱法和 DNA 的察觉，限制碎片长度多型性分析，甲基化 specific PCR，和免疫组织化学的染色被使用。结果：倡导者多型性不是在激活的电子恶棍的主要机制。仅仅一截断变化在一个弥漫的类型肿瘤(3%) 被发现。loh 和倡导者 hypermethylation 是在激活的电子恶棍的主要机制，但是有趣地，在 CDH1 的肿瘤和 hypermethylation 在突变而产生之遗传的损失(loh ) 的部分之间有一个否定协会。因此 loh 和 hypermethylation 是涉及 GC 的二条不同致瘤的小径。结论：给体细胞突变是极其低的，在 loh 和 hypermethylation 之间的关系是反的调查结果，这三个因素的任何二联合不能在激活完成 CDH1 的古典二点击的假设。因此，在 transcriptional 水平或在 translational 以后水平操作的另外的机制可能被要求在激活导致 E-cadherin。

关键词：胃癌基因表达病理机制临床

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二维材料最新研究进展

物理化学学报 2021年第12期37卷 1-151页

作者：常诚陈伟陈也陈永华陈雨丁峰樊春海范红金范战西龚成宫勇吉何其远洪勋胡晟胡伟达黄维黄元季威李德慧李连忠李强林立凌崇益刘鸣华刘楠刘庄 Kian Ping loh 马建民缪峰彭海琳邵明飞宋礼苏邵孙硕谭超良唐智勇王定胜王欢王金兰王欣王欣然 Andrew T.S.Wee 魏钟鸣吴宇恩吴忠帅熊杰熊启华徐伟高尹鹏曾海波曾志远翟天佑张晗张辉张其春张铁锐张翔赵立东赵美廷赵伟杰赵运宣周凯歌周兴周喻朱宏伟张华刘忠范 Institute of Science and Technology Austria Am Campus 13400 KlosterneuburgAustria Department of Chemistry National University of Singapore3 Science Drive 3117543Singapore Department of Chemistry The Chinese University of Hong KongShatinNew TerritoriesHong KongChina Key Laboratory of Flexible Electronics and Institute of Advanced Materials Nanjing Tech UniversityNanjing 211816China School of Life Sciences Shanghai UniversityShanghai 200444China Centre for Multidimensional Carbon Materials Institute for Basic ScienceUlsan 44919Korea School of Chemistry and Chemical Engineering Shanghai Jiao Tong UniversityShanghai 200240China School of Physical and Mathematical Sciences Nanyang Technological UniversitySingapore 637371Singapore Department of Chemistry City University of Hong KongKowloonHong KongChina Department of Electrical and Computer Engineering and Quantum Technology Center University of MarylandCollege ParkMaryland 20742USA School of Materials Science and Engineering Beihang UniversityBeijing 100191China Department of Materials Science and Engineering City University of Hong KongKowloonHong KongChina Center of Advanced Nanocatalysis(CAN) Hefei National Laboratory for Physical Sciences at the MicroscaleDepartment of Applied ChemistryUniversity of Science and Technology of ChinaHefei 230026China College of Chemistry and Chemical Engineering State Key Laboratory of Physical Chemistry of Solid SurfacesCollaborative Innovation Center of Chemistry for Energy Materials(iChEM)Xiamen UniversityXiamen361005Fujian ProvinceChina State Key Laboratory of Infrared Physics Shanghai Institute of Technical PhysicsChinese Academy of SciencesShanghai 200083China Advanced Research Institute of Multidisciplinary Science Beijing Institute of TechnologyBeijing100081China Beijing Key Laboratory of Optoelectronic Functional Materials&Micro-Nano Devices Department of PhysicsRenmin University of ChinaBeijing 100872China School of Optical and Electronic Information Wuhan National Laboratory

Research on two-dimensional(2D) materials has been explosively increasing in last seventeen years in varying subjects including condensed matter physics, electronic engineering, materials science, and chemistry since the mechanical exfoliation of graphene in 2004. Starting from graphene, 2D materials now have become a big family with numerous members and diverse categories. The unique structural features and physicochemical properties of 2D materials make them one class of the most appealing candidates for a wide range of potential applications. In particular, we have seen some major breakthroughs made in the field of 2D materials in last five years not only in developing novel synthetic methods and exploring new structures/properties but also in identifying innovative applications and pushing forward commercialisation. In this review, we provide a critical summary on the recent progress made in the field of 2D materials with a particular focus on last five years. After a brief backgroundintroduction, we first discuss the major synthetic methods for 2D materials, including the mechanical exfoliation, liquid exfoliation, vapor phase deposition, and wet-chemical synthesis as well as phase engineering of 2D materials belonging to the field of phase engineering of nanomaterials(PEN). We then introduce the superconducting/optical/magnetic properties and chirality of 2D materials along with newly emerging magic angle 2D superlattices. Following that, the promising applications of 2D materials in electronics, optoelectronics, catalysis, energy storage, solar cells, biomedicine, sensors, environments, etc. are described sequentially. Thereafter, we present the theoretic calculations and simulations of 2D materials. Finally, after concluding the current progress, we provide some personal discussions on the existing challenges and future outlooks in this rapidly developing field.

关键词： Two-dimensional materials Transition metal dichalcogenides Phase engineering of nanomaterials Electronics Optoelectronics Catalysis Energy storage and conversion

胃癌组织脆性组氨酸三联体基因杂合性丢失的临床意义

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中国误诊学杂志 2011年第23期11卷 5547-5548页

作者：许凡葛鹏岳晚霞西安医学院第二附属医院胸外科陕西西安710038 西安医学院第二附属医院病理科陕西西安710038

目的检测脆性组氨酸三联体(FHIT)基因在胃癌(GC)、不典型增生(DP)和肠上皮化生(IM)组织中的杂合性丢失(loss of heterozygosity,loh),研究loh在GC发生中的作用。方法采用PCR方法检测GC、DP和IM组织和正常对照胃黏膜组织中FHIT基因多态... 详细信息

目的检测脆性组氨酸三联体(FHIT)基因在胃癌(GC)、不典型增生(DP)和肠上皮化生(IM)组织中的杂合性丢失(loss of heterozygosity,loh),研究loh在GC发生中的作用。方法采用PCR方法检测GC、DP和IM组织和正常对照胃黏膜组织中FHIT基因多态性位点D3S1234和D3S1300中的loh。结果 GC组、DP组和IM组在D3S1234位点的loh发生率分别为33.0%,29.0%和11.0%;在D3S1300位点的loh发生率分别为34.0%,33.0%和8.0%。GC组和DP组在D3S1234和D3S1300位点的loh发生率显著高于IM组,GC组与DP组间差异无统计学意义。结论 FHIT基因的loh可能是GC形成过程中的早期事件,能用于GC高危人群筛查和早期诊断的研究。

关键词：胃肿瘤/遗传学基因,肿瘤抑制染色体脆性组氨酸/遗传学杂合子丢失

散发性结直肠癌1号染色体短臂遗传位点杂合缺失

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肿瘤 2007年第1期27卷 51-54页

作者：裘国强周崇治张放贺林彭志海上海市交通大学附属第一人民医院普通外科上海200080 上海交通大学Bio-X生命科学研究中心上海200030

目的:抑癌基因的杂合缺失(loss of heterozygosity,loh)被认为是结直肠癌形成的关键步骤之一。本实验研究了结直肠癌1号染色体短臂杂合的缺失情况,并探讨其临床意义。方法:选取11个微卫星DNA标记与83例结直肠癌病例的肿瘤和正常组织进行... 详细信息

目的:抑癌基因的杂合缺失(loss of heterozygosity,loh)被认为是结直肠癌形成的关键步骤之一。本实验研究了结直肠癌1号染色体短臂杂合的缺失情况,并探讨其临床意义。方法:选取11个微卫星DNA标记与83例结直肠癌病例的肿瘤和正常组织进行PCR。PCR产物在ABIPrism377自动荧光测序仪上进行电泳,以GeneScan3.1和Genotyper2.1软件进行遗传位点扫描以及杂合缺失分析。结果:1号染色体短臂的平均杂合缺失率为18.00%,D1S468(1p36.33-36.31)位点的杂合缺失率最高,达36.54%。D1S2726位点的杂合缺失现象主要存在于直肠癌,缺失率为28.57%(6/21),而结肠癌的缺失率为0.00%(0/33),二者差异具统计学意义(P=0.002)。结论:在1号染色体短臂上可能存在与结直肠癌发生相关的抑癌基因,位于1p36.33-36.31这个区域。

关键词：结肠直肠肿瘤杂合子丢失染色体,人 1对

16号染色体短臂散发性结直肠癌相关基因的精细定位

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肿瘤 2005年第6期25卷 573-576页

作者：黄力张放郑海涛裘国强贺林彭志海上海市第一人民医院普外科上海200080 上海交通大学Bio-X生命科学研究中心上海200032

目的对散发性结直肠癌16号染色体短臂杂合缺失区间(loh)进行精细定位,以期发现新的肿瘤相关基因.方法用覆盖16号染色体短臂的5个微卫星标记对83例散发性结直肠癌进行杂合缺失分析,初步定位候选区域后再用另5个微卫星标记对这一区域进行... 详细信息

目的对散发性结直肠癌16号染色体短臂杂合缺失区间(loh)进行精细定位,以期发现新的肿瘤相关基因.方法用覆盖16号染色体短臂的5个微卫星标记对83例散发性结直肠癌进行杂合缺失分析,初步定位候选区域后再用另5个微卫星标记对这一区域进行精细定位.PCR扩增相应位点的基因组DNA,并用ABI 377自动测序仪进行电泳.用Genescan 3.7 和Genotype 3.7软件进行遗传位点扫描及杂合缺失分析.杂合缺失与临床病理参数的关系比较采用χ2检验.结果 16号染色体短臂的平均杂合缺失率为24.58%,仅有一高频缺失位点D16S404,杂合缺失率达52.73%.对其精细定位后发现,最小杂合缺失区间应位于D16S406和D16S3126之间,约1.1 cM.而这一区间的杂合缺失与Dukes分期、肿瘤分化及淋巴结转移等无关.结论通过散发性结直肠癌16号染色体短臂杂合缺失的精细定位研究,发现了D16S406～D16S3126之间精度达1.1 cM的肿瘤相关基因候选区域.在这一区间内可能的肿瘤抑制基因为USP7基因,相邻的候选肿瘤抑制基因有EMP2.对这2个基因的深入研究可能明确新的散发性结直肠癌相关基因.

关键词：结肠直肠肿瘤基因,肿瘤抑制染色体,人,16对杂合子丢失

他达那非联合十一酸睾酮治疗中老年男性勃起功能障碍伴迟发性性腺功能减退症的研究

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实用老年医学 2011年第1期25卷 37-39页

作者：石亮孙超徐志鹏南京大学医学院附属鼓楼医院泌尿男科江苏省南京市210008

目的观察他达那非联合十一酸睾酮治疗中老年男性勃起功能障碍(ED)伴迟发性性腺功能减退症(loh)患者的临床疗效。方法将符合标准的45例患者分为2组,研究组23例,采用他达那非联合十一酸睾酮的治疗方案,对照组22例,单用他达那非治疗。4周后... 详细信息

目的观察他达那非联合十一酸睾酮治疗中老年男性勃起功能障碍(ED)伴迟发性性腺功能减退症(loh)患者的临床疗效。方法将符合标准的45例患者分为2组,研究组23例,采用他达那非联合十一酸睾酮的治疗方案,对照组22例,单用他达那非治疗。4周后,应用国际勃起功能指数评分-5(IIEF-5)、老年男性症状问卷(AMS)比较2组的治疗效果。结果 2组治疗后IIEF-5评分较治疗前增加(P均loh患者的治疗中,联合应用他达那非和十一酸睾酮的治疗效果优于单用他达那非的效果。

关键词：勃起功能障碍迟发性性腺功能减退症他达那非十一酸睾酮