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抑制RAb27a能够抑制三阴乳腺癌细胞的增殖、侵袭和粘附

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南方医科大学学报 2023年第4期43卷 560-567页

作者：王丽严志锐夏耀雄云南省肿瘤医院//昆明医科大学第三附属医院放射治疗科云南昆明650118

目的抑制在外泌体分泌过程中发挥核心作用的RAb27蛋白家族的表达,以探究其对三阴性乳腺癌(TNbC)细胞生物学功能的影响。方法利用荧光定量PCR和蛋白印迹法对比3株三阴性乳腺癌细胞系MDA-Mb-231、MDA-Mb-468、Hs578T和正常乳腺上皮细胞系M... 详细信息

目的抑制在外泌体分泌过程中发挥核心作用的RAb27蛋白家族的表达,以探究其对三阴性乳腺癌(TNbC)细胞生物学功能的影响。方法利用荧光定量PCR和蛋白印迹法对比3株三阴性乳腺癌细胞系MDA-Mb-231、MDA-Mb-468、Hs578T和正常乳腺上皮细胞系MCF10A的RAb27家族基因和蛋白表达以及外泌体的分泌情况;使用siRNA干扰技术分别抑制3株三阴性乳腺癌细胞中RAb27a和RAb27b的表达,并采用荧光定量PCR和蛋白印迹法检测抑制效率,随后使用蛋白印迹法检测这3株三阴性乳腺癌细胞RAb27a和RAb27b分别被抑制后外泌体的分泌情况。用细胞增殖、细胞侵袭和细胞粘附实验评价RAb27a和RAb27b分别被抑制后对这3株三阴性乳腺癌细胞生物学功能造成的影响。结果三阴性乳腺癌细胞和正常乳腺上皮细胞相比外泌体分泌更加旺盛(Pb27a和RAb27b的表达在基因和蛋白水平上均显著上升(Pb27a的表达后,外泌体分泌显著下调(Pb27b的表达对外泌体分泌无显著影响。并且外泌体分泌显著下调后,与外泌体分泌未受到显著影响的三阴性乳腺癌细胞相比,癌细胞增殖能力、细胞侵袭能力和细胞粘附能力均显著下调(Pb27a,抑制RAb27a能够抑制三阴性乳腺癌细胞的增殖、侵袭和粘附。

关键词： RAb27a RAb27b 外泌体三阴性乳腺癌细胞生物学功能

提速干线编组站出发子系统内部匹配与协调关系

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中国铁道科学 2006年第5期27卷 118-121页

作者：朱晓立李夏苗北京交通大学交通运输学院北京100044 中南大学交通运输工程学院湖南长沙410075

分析提速干线编组站货物列车到发时刻不均衡运营特征,根据编组站作业特点和要求,运用排队论和随机过程理论,对出发子系统转场和接车可靠性、临界密集出发时间进行计算分析。认为出发子系统允许的接车延误概率应控制在0.12以内;当区间通... 详细信息

分析提速干线编组站货物列车到发时刻不均衡运营特征,根据编组站作业特点和要求,运用排队论和随机过程理论,对出发子系统转场和接车可靠性、临界密集出发时间进行计算分析。认为出发子系统允许的接车延误概率应控制在0.12以内;当区间通过能力利用率在繁忙时间达0.85以上时,出发场的到发线应在10股以上。提高出发子系统转场和接车可靠性的措施包括:提高无调比、适当增加出发场到发线数量、降低编组调车机车的作业负荷、减小出发时间间隔不均衡系数。给出一定出发强度下的临界密集发车时间计算式,便于指导运输组织工作。改善出发子系统匹配与协调关系的有效途径包括:合理配置列检组数、转线接车延误率控制在10%以内、密集到发期间区段通过能力利用率不能超过90%、降低衔接区间发车时间间隔变异系数、连续高密集发车时间不能超过临界发车时间、适当增加分类线及到发线数量、优化列车运行计划、平衡双向系统作业负荷、加强管理、优化运输组织。

关键词：编组站出发子系统列车到发时刻排队论随机过程

HLA-A,-b及-DRb1等位基因的多态性与白血病易感性的关联

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细胞与分子免疫学杂志 2005年第5期21卷 619-621页

作者：周兰霞赵丽兰州大学第一医院中心实验室甘肃兰州730000

目的:探讨甘肃汉族人群中白血病的易感性与HLA基因多态性之间的关联。方法:采用序列特异性寡核苷酸探针杂交技术(PCR-SSO),对57例白血病患者和45例健康对照组进行HLA-A、-b及-DRb1基因分型。结果:在等位基因HLA-A、-b及-DRb1中,白血病... 详细信息

目的:探讨甘肃汉族人群中白血病的易感性与HLA基因多态性之间的关联。方法:采用序列特异性寡核苷酸探针杂交技术(PCR-SSO),对57例白血病患者和45例健康对照组进行HLA-A、-b及-DRb1基因分型。结果:在等位基因HLA-A、-b及-DRb1中,白血病患者的HLA-A01、-b38及-DR15基因的基因频率高于正常对照组(Pb38及-DR15对白血病具有遗传易感作用;而基因HLA-A11和-DR03对白血病具有遗传拮抗作用。

关键词： HLA 白血病 PCR-SSO 多态性

The enhancement of Arsenic Trioxideby Trichostatin A in inducing differentiation of HL-60 cells

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The enhancement of Arsenic Trioxideby Trichostatin A in indu...

MRI及MR扩散成像在评价大脑半球皮质弥漫性病变的价值

第十届全国免疫学学术大会

作者： Gaofeng bi xia Ren Guanhua Song Lianlian Li xiaoyu Zhang Guosheng Jiang Institute of basic Medicine Shandong Academy of Medical Sciences

Objective:In order to study if Trichostatin A facilitate Arsenic Trioxide to induce differentiation of HL-60 cells and its underlying ***:the alteration in the cell proliferation was determined by MTT *** reduction assay and CD11b analysis by FACS were used to detect the differentiation of HL60 *** cycle arrest was measured by *** expressions of m RNA of P21,P27 and Cyclin D3,Cyclin E in HL60 cells after exposure to TSA and(or)Arsenic Trioxide were evaluated by semi-quantitative ***:It was found that the combinationof TSAand Arsenic Trioxide could inhibit proliferation and induce differentiation of HL60 cells obviously than Arsenic Trioxide *** up-regulationof the m RNA expression of P21,P27 but down-regulation of the expression of Cyclin D3,Cyclin E were related to the differentiation of ***:TSA has synergistic effect with Arsenic Trioxide in inducing differentiation of HL60 and it’s associated with the change of P21,P27 and Cyclin D3,Cyclin E expression.

关键词： Trichostatin A Arsenic Trioxide HL-60 cell induce differentiation synergistic effect

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中华医学杂志 2011年第13期91卷 880-884页

作者：夏爽马国林天津市第一中心医院放射科 300192 卫生部中日友好医院放射科

目的探讨MRI及MR扩散成像在评价大脑半球皮质弥漫性病变的价值.方法收集2006年1月至2009年5月天津市第一中心医院大脑半球弥漫性病变患者19例,其中男9例、女10例,年龄范围16～70岁,平均年龄45岁.所有患者均于Philips 1.5 T MR[扫描机... 详细信息

目的探讨MRI及MR扩散成像在评价大脑半球皮质弥漫性病变的价值.方法收集2006年1月至2009年5月天津市第一中心医院大脑半球弥漫性病变患者19例,其中男9例、女10例,年龄范围16～70岁,平均年龄45岁.所有患者均于Philips 1.5 T MR[扫描机上进行.SE-EPIDWI序列,TR/TE 6225 ms/118.7 ms,ETL 128,FLIP 90°,层厚5 mm,矩阵128×128,b值1000 mm2/s.结果 3例CJD患者,4例聚氯乙烯黏合剂中毒患者,2例病毒性脑炎患者,3例肝衰竭相关的脑水肿患者,1例一氧化碳中毒迟发脑病患者,DWI和ADC均呈高信号,而在随访的MRI研究中,大部分病变恢复较快,DWI高信号消失.4例窒息后昏迷、2例H2S中毒患者的DWI呈高信号,ADC呈低信号.结论 DWI能够更早期敏感的显示大脑半球弥漫性病变的部位和范围,尤其是扩散受限的病变.DWI呈显著高信号,ADC呈低信号,提示细胞毒性水肿.当DWI和ADC均呈高信号时,显示病变的T2效应,提示血管源性水肿.

关键词：磁共振成像扩散大脑

二次阳极氧化对TiO_2纳米管阵列形貌及光电催化性能的影响

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人工晶体学报 2015年第11期44卷 3124-3128页

作者：林晓霞刘佳付德刚金陵科技学院材料工程学院南京211169 东南大学生物电子学国家重点实验室南京210096

在含水量为2wt%的0.3wt%NH4F和乙二醇电解液中,通过二次阳极氧化法制备了高度有序的Ti O_2纳米管阵列(TNT)。利用扫描电镜(SEM)、X射线衍射(XRD)表征方法对TiO_2纳米管阵列的形貌、晶型进行表征。结果表明,二次阳极氧化法形成的TNT更加... 详细信息

在含水量为2wt%的0.3wt%NH4F和乙二醇电解液中,通过二次阳极氧化法制备了高度有序的Ti O_2纳米管阵列(TNT)。利用扫描电镜(SEM)、X射线衍射(XRD)表征方法对TiO_2纳米管阵列的形貌、晶型进行表征。结果表明,二次阳极氧化法形成的TNT更加规整,纳米管孔径大小一致。XRD分析显示二次阳极氧化法形成的TNT锐钛矿相衍射峰更强。光催化降解实验表明,二次阳极氧化制备的TNT对X-3b的降解效率是一次阳极氧化TNT的1.45倍。

关键词：二次阳极氧化 TiO2纳米管阵列光电催化性能

Equivalent elastic compliance tensor for rock mass with multiple persistent joint sets:Exact derivation via modified crack tensor

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Journal of Central South University 2016年第6期23卷 1486-1507页

作者：崔洁江权冯夏庭李邵军高红李帅军 State Key Laboratory of Geomechanics and Geotechnical Engineering Institute of Rock and Soil MechanicsChinese Academy of Sciences

Discontinuities constitute an integral part of rock mass and inherently affect its anisotropic deformation *** work focuses on the equivalent elastic deformation of rock mass with multiple persistent joint sets.A new method based on the space geometric and mechanical properties of the modified crack tensor is proposed,providing an analytical solution for the equivalent elastic compliance tensor of rock mass.A series of experiments validate the capability of the compliance tensor to accurately represent the deformation of rock mass with multiple persistent joint sets,based on conditions set by the basic *** spatially varying rules of the equivalent elastic parameters of rock mass with a single joint set are analyzed to reveal the universal law of the stratified rock mass.

关键词： equivalent elastic compliance tensor crack tensor multiple joint sets rock mass

The synthesis and crystal structure of metal complexes S-alkyl-N-(ferrocenyl-1-methyl-methylidene)-dithiocarbazate and the study on their quenching the luminescence ofruthenium(bipyridine)_~3(2+)

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Chinese Journal of Chemistry 1994年第4期12卷 321-327页

作者： DUAN, Chun-ying xia, Xu-bing ZHU, Long-Gen yOU, xiao-Zeng yANG, yao WANG, Hua-QinCoordination Chemistry Institute, Nanjing University The State Key Laboratory of Coordination Chemistry, Nanjing, Jiangsu 210008, China Coordination Chemistry Institute Nanjing University The State Key Laboratory of Coordination Chemistry Nanjing Jiangsu 210008 China

The cobalt, nickel, copper, zinc and cadmium complexes of S-methyl-N-(ferrocenyl-l-methyl-methylidene)-dithiocarbazate (H-LSM) and S-benzyl-N-(ferrocenyl-l-methyl-methylidene)-dithiocarbazate (H-LSb) were synthesized and the crystal structure of Cd[Fe-C(CH3) = NNCSS. (CH3)]2 was solved by X-ray diffraction. The crystal is in the orthorhombic system with space group Pbca, cell parameters a=19.741(3), b=19.924(5), c=15.452(4) A, and the final factors of R=0.032. The study on quenching the luminescence of Ru(bpy)3 by those complexes showed that bimolecular quenching constants obtained from the Stern-Vohner constant and the excited-state lifetime were related to the redox potential of the quencher. Linear relationship is shown in the plot of logkq vs. E1/2(Q+/Q). The main factor which influences the quenching rate constant and the redox potential is the coordinating ability of the metal in the complex.

关键词： Cadmium complex quenching constant electron transfer.

Syndecan 4-c-ros oncogene 1 fusion as a mechanism of acquired resistance in epidermal growth factor receptor mutant lung adenocarcinoma

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Chinese Medical Journal 2019年第24期132卷 3015-3017页

作者： you-Cai Zhu Chun-Wei Xu Qu-xia Zhang Wen-xian Wang Lei Lei Wu Zhuang Department of Thoracic Disease Center Zhejiang Rongjun HospitalJiaxingZhejiang 314000China Department of Pathology Fujian Cancer HospitalFujian Medical University Cancer HospitalFuzhouFujian 350014China Department of Chemotherapy Zhejiang Cancer HospitalHangzhouZhejiang 310022China Department of Medical Thoracic Oncology Fujian Cancer HospitalFujian Medical University Cancer HospitalFuzhouFujian 350014China.

To the Editor:A 37-year-old Chinese woman,a nonsmoker,presented with adenocarcinoma of the left lung(stage IV)[Figure 1A],malignant pleural effusion,and multiple bone metastases in December *** acid sequencing of the tumor biopsy revealed an epidermal growth factor receptor(EGFR)exon 19 deletion,and treatment with gefitinib was consequently initiated in December *** were no treatmentrelated side effects except for a grade 1 rash,and stable disease was ***,the patient did not experience long-term benefits and developed disease progression after only two months.A follow-up examination showed that extracranial disease and a small intracranial lesion had developed *** patient then received whole-brain radiotherapy(30 Gy in ten fractions)and four cycles of single-agent pemetrexed in April *** disease was achieved,which coincided with an improvement in the patient’s cough,shortness of breath,and general *** chemotherapy course was changed to two cycles of pemetrexed combined with cisplatin.A partial response(PR)was achieved,and the patient was treated with 14 cycles of pemetrexed alone as maintenance *** follow-up evaluations showed the patient’s condition to be stable;however,the patient’s disease progressed after 23 months.A computed tomography(CT)scan in March 2017 revealed that both the lung lesion and the malignant pleural effusion had increased in size[Figure 1b].The subsequent biopsy specimen was subjected to next-generation sequencing(NGS)and a Syndecan 4-c-ros oncogene 1(SDC4-ROS1)rearrangement was detected[Figure 1C].The patient then received crizotinib in April 2017,and a PR was achieved[Figure 1D].A CT scan done in August 2018 indicated the progression of the primary lesion in the left lung and malignant pleural ***,the growth of the remaining lesions remained stable.

关键词： adenocarcinoma Figure smoke