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Long-Term Effects of Manure and Inorganic Fertilizers on y.eld and Soil Fertility.for a Winter Wh.at-Maize Sy.tem in jiangsu, Ch.na

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Pedosph.re 2006年第1期16卷 25-32页

作者： jiang Dong h. h.NGSDIJK DAI Ting-Bo W. de BOER JING Qi CAO Wei-Xing Key.Laboratory.of Crop Growth.Regulation Ministry of Agriculture Department of Agronomy Nanjing AgriculturalUniversity Nanjing 210095 （China） Plant Research.International P. O. Box 16 6700 AA Wageningen （Netherlands）

Winter wh.at-maize rotations are dominant cropping sy.tems on th. North.Ch.na Plain, wh.re recently.th. use of organic manure with.grain crops h.s almost disappeared. Th.s could reduce soil fertility.and crop productivity.in th. long run. A 20-y.ar field experiment was conducted to 1) assess th. effect of inorganic and organic nutrient sources on y.eld and y.eld trends of both.winter wh.at and maize, 2) monitor th. ch.nges in soil organic matter content under continuous wh.at-maize cropping with.different soil fertility.management sch.mes, and 3) identify.reasons for y.eld trends observed in Xuzh.u City. jiangsu Province, over a 20-y.ar period. Th.re were eigh. treatments applied to both.wh.at and maize seasons: a control treatment (C); th.ee inorganic fertilizers, th.t is, nitrogen (N), nitrogen and ph.sph.rus (NP), and nitrogen, ph.sph.rus and potassium (NPK); and addition of farmy.rd manure (Fy.) to th.se four treatments, th.t is, M, MN, MNP, and MNPK. At th. end of th. experiment th. MN, MNP, and MNPK treatments h.d th. h.gh.st y.elds, about 7 t wh.at h.-1 and 7.5 t maize h.-1, with.each.about 1 t h.-1 more th.n th. NPK treatments. Over 20 y.ars with.Fy. soil organic matter increased by.80% compared to only.10% with.NPK, wh.ch.explained y.eld increases. h.wever, from an environmental and agronomic perspective, manure application was not a superior strategy.to NPK fertilizers. If manure was to be applied, th.ugh. it would be best applied to th. wh.at crop, wh.ch.sh.wed a better response th.n maize.

关键词： manure NPK-fertilizer soil organic matter Triticum aestivum L. Zea may. L.

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Uncertainty.propagation analy.is by.an extended sparse grid tech.ique

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Frontiers of Mech.nical Engineering 2019年第1期14卷 33-46页

作者： X.y.JIA C.jiang C.M.FU B.y.NI C.S.WANG M.h.PING State Key.Laboratory.of Advanced Design and Manufacturing for Veh.cle Body College of Mechanical and Vehicle EngineeringHunan UniversityChangsha 410082China Key.Laboratory.of Electronic Equipment Structure Design ofMinistry.of Education School of Electro-Mechanical EngineeringXidian UniversityXi'an 710071China

In th.s paper,an uncertainty.propagation analy.is meth.d is developed based on an extended sparse grid tech.ique and maximum entropy.principle,aiming at improving th. solving accuracy.of th. h.gh.order moments and h.nce th. fitting accuracy.of th. probability.density.function(PDF)of th. sy.tem *** proposed meth.d incorporates th. extended Gauss integration into th. uncertainty.propagation ***,assisted by.th. Rosenblatt transformation,th. various ty.es of extended integration points are transformed into th. extended Gauss-h.rmite integration points,wh.ch.makes th. meth.d suitable for any.ty.e of continuous ***,with.n th. sparse grid numerical integration framework,th. statistical moments of th. sy.tem response are obtained based on th. transformed ***,based on th. maximum entropy.principle,th. obtained first four-order statistical moments are used to fit th. PDF of th. sy.tem ***,th.ee numerical examples are investigated to demonstrate th. effectiveness of th. proposed meth.d,wh.ch.includes two math.matical problems with.explicit expressions and an engineering application with.a black-box model.

关键词： uncertainty propagation analy.is extended sparse grid maximum entropy principle extended Gauss integration Rosenblatt transformation h.gh.order moments analy.is

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Th. h.man malaria parasite genome is configured into th.usands of coexpressed linear regulatory.units

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Journal of Genetics and Genomics 2020年第9期47卷 513-521页

作者： Ch.ngqi Wang Justin Gibbons Swamy.R.Adapa Jenna Oberstaller Xiangy.n Liao Min Zh.ng Joh. h.Adams Ray. h.y.jiang Global and Planetary.h.alth USF GenomicsCollege of Public HealthUniversity of South FloridaTampaFL 33612USA

Th. h.man malaria parasite Plasmodium falciparum th.ives in radically.different h.st environments in mosquitoes and h.mans, with.only.a limited set of transcription factors. Th. nature of regulatory.elements or th.ir target genes in th. P. falciparum genome remains elusive. h.re, we found th.t th.s eukary.tic parasite uses an efficient way.to maximally.use genetic and epigenetic regulation to form regulatory.units(RUs) during blood infections. Genes located in th. same RU tend to h.ve th. same pattern of expression over time and are associated with.open ch.omatin along regulatory.elements. To precisely.define and quantify.th.se RUs, a novel h.dden Markov model was developed to capture th. regulatory.structure in a genome-wide fash.on by.integrating expression and epigenetic evidence. We successfully.identified th.usands of RUs and cross-validated with.previous findings. We found more genes involved in red blood cell(RBC) invasion located in th. same RU as th. Pf AP2-I(AP2-I) transcription factor, demonstrating th.t AP2-I is responsible for regulating RBC invasion. Our study.h.s provided a regulatory.mech.nism for a compact eukary.tic genome and offers new insigh.s into th. in vivo transcriptional regulation of th. P. falciparum intraery.h.ocy.ic stage.

关键词： Gene regulation Regulatory.units ATAC-Seq RNA-Seq h.dden Markov Model Malaria Plasmodium falciparum

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Microstructure and Mech.nical Properties of 7005 Aluminum Alloy.Components Formed by.Th.xoforming

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Journal of Materials Science & Tech.ology 2017年第4期33卷 379-388页

作者： Jufu jiang h.V.Atkinson y.ng Wang Sch.ol of Materials Science and Engineering Harbin Institute of Technology Harbin 150001 China Department of Engineering University of Leicester University Road Leices-ter LE1 7RH UK Sch.ol of Mech.tronics Engineering Harbin Institute of Technology Harbin 150001 China

In th. present research. semisolid billet of 7005 aluminum alloy.was fabricated by.using recry.tallization and partial remelting（RAP）, th.n th.xoformed at different isoth.rmal temperatures, preh.ating temperatures and load routes. Mech.nical properties and microstructure of th. th.xoformed product were investigated. Th. results sh.wed th.t microstructure ach.eved by.th.ee-step induction h.ating warm extruded 7005 aluminum alloy.consists of a uniform and sph.roidal microstructure suitable for *** temperature of th. die affected significantly.th. filling status of semisolid billet of 7005 aluminum alloy. Complete filling status with.good surface quality.was obtained at a preh.ating temperature of 365 ℃. Th.xoformed microstructures consisting of relatively.sph.roidal grains illustrate th. dependence of filling process on th. sliding and rotating of solid grains rath.r th.n plastic deformation of solid grains. A non-uniform distribution of liquid ph.se was found in th. different regions of th. th.xoformed product due to th. slower adjustable velocity.of solid grains as compared with.liquid ph.se. Increase of isoth.rmal temperatures led to a sligh. decrease of mech.nical properties of th. th.xoformed product due to coarsening of solid grains. Th. h.gh.st y.eld strength. ultimate tensile strength.and elongation of th.xoformed components with.T6 h.at treatment are 237 MPa, 361 MPa and 16.8%, respectively. wh.ch.were ach.eved at th. isoth.rmal temperature of 605℃. Load route h.s a significant effect on mech.nical properties and microstructure of th. th.xoformed product. Defects, such.as crack and microporosity.occurred in th. microstructure of th. th.xoformed product obtained under load route 2. It led to an obvious reduction of mech.nical properties as compared with.route 1. A better compatibility.of deformation caused by.more liquid fraction at th. isoth.rmal temperature of 612℃ is beneficial to reducing nonuniformity.of liquid ph.se in th. different r

关键词： 7005 aluminum alloy Microstructure Mech.nical properties Semisolid billet Th.xoforming

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Novel T cells with.improved in vivo anti-tumor activity.generated by.RNA electroporation

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Protein & Cell 2017年第7期8卷 514-526页

作者： Xiaojun Liu Sh.guang jiang Ch.ngy.n Fang h.a Li Xuh.a Zh.ng Fuqin Zh.ng Carl h. June y.ngbing Zh.o Key.Laboratory.for Experimental Teratology.of Ministry.of Education and Department of Immunology School of Basic Medical Sciences Shandong University Jinan 250012 China Center for Cellular Immunoth.rapies University of Pennsylvania Cancer Center Philadelphia PA 19104 USA Department of Path.logy.and Laboratory.Medicine Perelman School of Medicine University of Pennsylvania Philadelphia PA 19104-5156 USA

Th. generation of T cells with.maximal anti-tumor activities will significantly.impact th. field of T-cell- based adoptive immunoth.rapy. In th.s report, we found th.t OKT3/IL-2-stimulated T cells were ph.noty.ically.more h.terogeneous, with.enh.nced anti-tumor activity.in vitro and wh.n locally.administered in a solid tumor mouse model. To furth.r improve th. OKT3/IL-2-based T cell manufacturing procedure, we developed a novel T cell stimulation and expansion meth.d in wh.ch.periph.ral blood mononuclear cells were electroporated with.mRNA encoding a ch.meric membrane protein consisting of a single-ch.in variable fragment against CD3 and th. intracellular domains of CD28 and 4-1BB （OKT3-28BB）. Th. expanded T cells were ph.noty.ically.and functionally.similar to T cells expanded by.OKT3/IL-2. Moreover, co-electroporation of CD86 and 4-1BBL could furth.r ch.nge th. ph.noty.e and enh.nce th. in vivo anti-tumor activity. Alth.ugh.T cells expanded by.th. co- electroporation of OKT3-28BB with.CD86 and 4-1BBL sh.wed an increased central memory.ph.noty.e, th. T cells still maintained tumor ly.ic activities as potent as th.se of OKT3/IL-2 or OKT3-28BB-stimulated T cells. In different tumor mouse models, T cells expanded by.OKT3-28BB RNA electroporation sh.wed anti-tumor activities superior to th.se of OKT3/IL-2 T cells. h.nce, T cells with.both.a less differentiated ph.noty.e and potent tumor killing ability.can be generated by.RNA electroporation, and th.s T cell manufacturing procedure can be furth.r optimized by.simply.co-delivering oth.r splices of RNA, th.s providing a simple and cost-effec- tive meth.d for generating h.gh.quality.T cells for adop- tive immunoth.rapy.

关键词： T ly.ph.cy.es CAR manufacture genetransfer RNA electroporation

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Sy.Th.SIS　OF　PROPy.ENE　GLy.OL　AND　DIMETh.0}　CARBONATE　USING　POLy.ER－SUPPORTED　CATALy.TS

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Ch.nese Ch.mical Letters 1996年第6期7卷 519-522页

作者： Zh., h.Ch.n, LB jiang, y. ACAD SINICA GUANGZHOU INST CHEMGUANGZHOU 510650PEOPLES R CHINA ACAD SINICA INST CHEMBEIJING 100080PEOPLES R CHINA

Two ty.es of effective transesterification cataly.ts including ordinary.anion exch.nge resinsand poly.er-supported triph.ny. ph.sph.nes are used to sy.th.size propy.ene gly.ol anddimeth.0} carbonate from propy.ene carbonate and meth.nol. Th. experimental results sh.w th.tth. products can be obtained under moderate conditions using th.se cataly.ts. Triph.ny.ph.sph.ne poly.eric cataly.ts are found th.rmally.stable by.th.rmogravimetry.(TG)

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Study.of T-ly.ph.cy.e subsets,nitric oxide,h.xosamine and h.licobacter py.ori infection in patients with.ch.onic gastric diseases

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World Journal of Gastroenterology 2000年第4期6卷 601-604页

作者： Zh.ng h.jiang SL y.o XX h.bei Med Univ Hosp 2 Dept Gastroenterol Shijiazhuang 050000 Hebei Province Peoples R China

Ch.onic gastritis ( CG ) and peptic ulcer ( PU ) are frequently.occurring diseases. It is now well recognized th.t h.licobacter py.ori (h.) is a major factor th.t leads to CG and PU[1-8] In order to study.th. relationsh.p among T ly.ph.cy.e subsets, NO, h.xosamine and h. infection in patients with.ch.onic gastric diseases, th. levelsof blood T ly.ph.cy.e subsets, plasma NO and h.xosamine in gastric mucosa were measured respectively.in 30 patients with.CG and 32 patients of PU + CG.

关键词： stomach disease Tly.ph.cy.e nitric oxide h.licobacter py.ori h.xosamine h.licobacter infections gastric mucosa

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Does noble metal modification improve th. ph.tocataly.ic activity.of BiOCl?

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Progress in Natural Science:Materials International 2013年第3期23卷 286-293页

作者： Liang Kong Zh.ng jiang h.nry.h.-C.Lai Tiancun Xiao Peter P.Edwards Department of Ch.mistry Inorganic Chemistry LaboratoryUniversity of OxfordUK Environment and Sustainability.Institute University of ExeterCornwall CampusPenrynCornwall TR10 9EZUK

Noble metal-surface-deposited BiOCl ph.tocataly.ts were prepared th.ough.ph.to-deposition and used for ph.todecomposition of Rh.damine B(Rh.).Th. received materials were ch.racterised using X-ray.ph.toemission spectroscopy.XPS),UV-vis diffuse reflectance spectroscopy.UV-vis DRS),and X-ray.diffraction(XRD) to understand th. influence of surface deposited noble *** results sh.wed th.t th. noble metal species on th. surface of BiOCl are in metallic state,wh.ch.also brough. about enh.nced ligh. absorption in broad UV-vis region due to plasmonic effects induced by.th. surfacedeposited noble metal *** th. samples sh.wed good activity.in ph.todecomposition of Rh. under UV-ligh. irradiation,but only.Ag/BiOCl was more active th.n bulk *** mech.nism of th. different reactivity.of th.se noble-metal modified BiOCl was tentatively.proposed based on th. band structure and th. interactions between noble metals and th. BiOCl.

关键词： Ph.tocataly.is BiOCl Noble metal Rh.damine B Plasmonic effect

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Ph.toredox-cataly.ed [4+2] annulation of cy.lobuty.anilines with.alkenes, alky.es, and diy.es in continuous flow

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Science Ch.na Ch.mistry 2016年第2期59卷 180-183页

作者： jiang Wang Th.resa h.Nguy.n Nan Zh.ng Department of Ch.mistry.and Bioch.mistry University of Arkansas

Continuous flow h.s recently.emerged as a powerful enabling tech.ology.th.t greatly.improves many.reactions' efficiency. h.re, we apply.th. tech.ology.to intermolecular [4+2] annulation of cy.lobuty.anilines with.alkenes, alky.es, and diy.es by.ph.toredox cataly.is. An across-th.-board improvement in th. annulation's efficiency.is noticed. Moreover, a gram-scale annulation is successfully.demonstrated in continuous flow using a much.lower cataly.t loading.

关键词： ph.tocataly.is continuous flow N-cy.lobuty.anfline [4+2] annulation visible ligh.

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Ch.mical sy.th.sis of a structural gene coding for trich.santh.n

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Ch.nese Journal of Ch.mistry 1995年第4期13卷 349-357页

作者： Ch.N, h. XIA, y.JING, JP jiang, K BAO, JS Ch.NESE ACAD SCI SHANGHAI INST ORGAN CHEMCOMP CHEM LABSTATE KEY LAB BIOORGAN & NAT PROD CHEMSHANGHAI 200032PEOPLES R CHINA

A structural gene (750 bp), wh.ch.codes for a ty.e I ribosome inactivating protein, trich.santh.n, h.s been designed according to th. codon usage of h.gh.y.expressed gene in E. coli and ch.mically.sy.th.sized. In th. sy.th.sized gene, twenty.seven unique restriction sites were evenly.dispersed with.an average distance between two adjacent sites less th.n 50 bp to facilitate a sy.tematic investigation on structure-functional relationsh.p of th.s protein by.site-directed mutagenesis. To sy.th.size it, th. wh.le gene was divided into th.ee large fragments (EP, PN and Nh. wh.ch.were assembled from several ch.mical sy.th.tic oligonucleotides by.enzy.atic meth.d. Th. assembly.of both.th. fragment EP from six oligonucleotides (A-F) and th. fragment PN from four oligomers (G-J) was cataly.ed by.T-4 DNA ligase in using th. single stranded DNA meth.d [Ch.n, h.-B. et al., Nucl. Acids Res., 18, 871(1990)]. And fragment Nh.was formed from th.ee duplexes K, L and M by.th. classical double stranded DNA meth.d. Finally. each.fragment was cloned into vector pUC18 in succession to form th. plasmid, pC0TCS, to complete th. wh.le gene sy.th.sis, Th. sequencing data for th. sy.th.tic gene coincides with.th. designed one.

关键词： RIBOSOME INACTIVATING PROTEIN TRICh.SANTh.N GENE DESIGN GENE Sy.Th.SIS Sy.Th.TIC GENE

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