检索结果-南通市图书馆

Natural variation in the transcription factor REPLUMLESS contributes to both disease resistance and plant growth in Arabidopsis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Plant Communications 2022年第5期3卷 186-198页

作者： Miqi Xu xuncheng wang Jing Liu Aolin Jia Chao Xu Xing wang Deng Guangming He School of Life Sciences and School of Advanced Agricultural Sciences State Key Laboratory of Protein and Plant Gene ResearchPeking-Tsinghua Center for Life SciencesPeking UniversityBeijing 100871China Beijing Key Laboratory of Environment Friendly Management on Fruit Diseases and Pests in North China Institute of Plant ProtectionBeijing Academy of Agriculture and Forestry SciencesBeijing 100097China

When attacked by pathogens,plants need to reallocate energy from growth to defense to fend off the invaders,frequently incurring growth *** phenomenon is known as the growth–defense tradeoff and is orchestrated by a hardwired transcriptional *** key factors involved in this network has the potential to increase disease resistance without growth or yield loss,but the mechanisms underlying such changes require further *** conducting a genome-wide association study(GWAS)of leaves infected by the hemi-biotrophic bacterial pathogen Pseudomonas syringae ***(Pst)DC3000,we discovered that the Arabidopsis transcription factor REPLUMLESS(RPL)is necessary for bacterial *** importantly,RPL functions in promoting both disease resistance and *** analysis revealed a cluster of genes in the GRETCHEN HAGEN 3(GH3)family that were significantly upregulated in rpl mutants,leading to the accumulation of indole-3-acetic acid-aspartic acid(IAA-Asp).Consistent with this observation,transcripts of virulence effector genes were activated by IAA-Asp accumulated in the rpl *** found that RPL protein could directly bind to GH3 promoters and repress their *** also repressed flavonol synthesis by directly repressing CHI expression and thus activated the auxin transport pathway,which promotes plant ***,RPL plays an important role in plant immunity and functions in the auxin pathway to optimize Arabidopsis growth and defense.

关键词： natural variation GWAS REPLUMLESS bacterial resistance auxin

COP1 SUPPRESSOR 6 represses the PIF4 and PIF5 action to promote light-inhibited hypocotyl growth

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Integrative Plant Biology 2022年第11期64卷 2097-2110页

作者： Hongxia Lan Yueqin Heng Jian Li Mengdi Zhang Yeting Bian Li Chu Yan Jiang xuncheng wang Dongqing Xu Xing wang Deng Key Laboratory of Molecular Design for Plant Cell Factory of Guangdong Higher Education Institutes Department of BiologyInstitute of Plant and Food SciencesSchool of Life SciencesSouthern University of Science and TechnologyShenzhen 518055China State Key Laboratory of Crop Genetics and Germplasm Enhancement National Center for Soybean ImprovementCollege of AgricultureNanjing Agricultural UniversityNanjing 210095China Beijing Key Laboratory of Environment Friendly Management on Fruit Diseases and Pests in North China Institute of Plant and Environment ProtectionBeijing Academy of Agriculture and Forestry SciencesBeijing 100097China State Key Laboratory of Protein and Plant Gene Research Peking‐Tsinghua Center for Life SciencesSchool of Advanced Agriculture Sciences and School of Life SciencesPeking UniversityBeijing 100871China

Light signaling precisely controls photomorphogenic development in *** INTERACTING FACTOR 4 and 5(PIF4 and PIF5)play critical roles in the regulation of this developmental *** this study,we report CONSTITUTIVELY PHOTOMORPHOGENIC 1 SUPPRESSOR 6(CSU6)functions as a key regulator of light *** of CSU6 function largely rescues the cop1-6 constitutively photomorphogenic ***6 promotes hypocotyl growth in the dark,but inhibits hypocotyl elongation in the ***6 not only associates with the promoter regions of PIF4 and PIF5 to inhibit their expression in the morning,but also directly interacts with both PIF4 and PIF5 to repress their transcriptional activation ***6 negatively controls a group of PIF4-and PIF5-regulated gene *** in PIF4 and/or PIF5 are epistatic to the loss of CSU6,suggesting that CSU6 acts upstream of PIF4 and *** together,CSU6 promotes light-inhibited hypocotyl elongation by negatively regulating PIF4 and PIF5 transcription and biochemical activity.

关键词： COP1 CSU6 light signaling photomorphogenesis PIF4 PIF5

Green light mediates atypical photomorphogenesis by dual modulation of Arabidopsis phytochromes B and A

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Plant Biology 2024年第9期66卷 1915-1933页

作者： Miqi Xu Yi‐Yuan wang Yujie Wu Xiuhong Zhou Ziyan Shan Kunying Tao Kaiqiang Qian xuncheng wang Jian Li Qingqing Wu Xing wang Deng Jun‐Jie Ling National Engineering Laboratory of Crop Stress Resistance Breeding School of Life SciencesAnhui Agricultural UniversityHefei 230036China Province Key Laboratory of Resource Insect Biology and Innovative Utilization School of Life SciencesAnhui Agricultural UniversityHefei 230036China Biotechnology Center State Key Laboratory of Tea Plant Biology and UtilizationSchool of Tea and Food Sciences and TechnologyAnhui Agricultural UniversityHefei 230036China Beijing Key Laboratory of Environment Friendly Management on Fruit Diseases and Pests in North China Institute of Plant and Environment ProtectionBeijing Academy of Agriculture and Forestry SciencesBeijing 100097China College of Life Sciences Nanjing Normal UniversityNanjing 210023China State Key Laboratory of Protein and Plant Gene Research Peking–Tsinghua Center for Life SciencesSchool of Advanced Agriculture Sciencesand School of Life SciencesPeking UniversityBeijing 100871China State Key Laboratory of Wheat Improvement Shandong Laboratory of Advanced Agricultural Sciences at WeifangPeking University Institute of Advanced Agricultural SciencesWeifang 261000China

Although green light(GL)is located in the middle of the visible light spectrum and regulates a series of plant developmental processes,the mechanism by which it regulates seedling development is largely *** this study,we demonstrated that GL promotes atypical photomorphogenesis in Arabidopsis thaliana via the dual regulations of phytochrome B(phyB)and *** the Pr-to-Pfr conversion rates of phyB and phyA under GL were lower than those under red light(RL)in a fluence rate-dependent and time-dependent manner,long-term treatment with GL induced high Pfr/Pr ratios of phyB and ***,GL induced the formation of numerous small phyB photobodies in the nucleus,resulting in atypical photomorphogenesis,with smaller cotyledon opening angles and longer hypocotyls in seedlings compared to *** abundance of phyA significantly decreased after short-and long-term GL *** determined that four major PHYTOCHROME-INTERACTING FACTORs(PIFs:PIF1,PIF3,PIF4,and PIF5)act downstream of phyB in GL-mediated cotyledon *** addition,GL plays opposite roles in regulating different *** example,under continuous GL,the protein levels of all PIFs decreased,whereas the transcript levels of PIF4 and PIF5 strongly increased compared with dark *** together,our work provides a detailed molecular framework for understanding the role of the antagonistic regulations of phyB and phyA in GL-mediated atypical photomorphogenesis.

关键词： cotyledon development green light photobody phytochrome PIF3

Natural variation of H3K27me3 modification in two Arabidopsis accessions and their hybrid

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Plant Biology 2016年第5期58卷 466-474页

作者： Mei Yang xuncheng wang Hao Huang Diqiu Ren Yan'e Su Pan Zhu Danmeng Zhu Liumin Fan Liangbi Chen Guangming He Xing wang Deng State Key Laboratory of Protein and Plant Gene Research Peking-Tsinghua Center for Life Sciences School of Advanced Agricultural Sciences and School of Life Sciences Peking University Tsinghua-Peking Joint Center for Life Sciences School of Life SciencesTsinghua University Department of Botany College of Life Sciences Hunan Normal University

Histone modifications affect gene expression, but the mechanism and biological consequence of natural variation in histone modifications remain unclear. Here, we generated genome-wide integrated maps of H3K27me3 modification and transcriptome for Col, C24 and their F1 hybrid. A total of 1,828 genomic regions showing variation in H3K27me3 modification between Col and C24 were identified, most of which were associated with genic regions. Natural variation of H3K27me3 modification between parents could result in aUelic bias of H3K27me3 in hybrids. Furthermore, we found that H3K27me3 variation between Col and C24 was negatively correlated with gene expression differences between two accessions, espe- cially with those arising from the cis-effect. Importantly, mutation of CLF, an Arabidopsis methyltransferase for H3K27,altered gene expression patterns between the parents. Together, these data provide insights into natural variation of histone modifications and their association with gene expression differences between Arabidopsis ecotypes.

关键词： Allelic bias Arabidopsis gene expression H3K27me3 natural variation

The GmSTF1/2–GmBBX4 negative feedback loop acts downstream of blue-light photoreceptors to regulate isoflavonoid biosynthesis in soybean

在线全文

学校读者我要写书评

暂无评论

Plant Communications 2024年第2期5卷 198-214页

作者： Zhaoqing Song Fengyue Zhao Li Chu Huan Lin Yuntao Xiao Zheng Fang xuncheng wang Jie Dong Xiangguang Lyu Deyue Yu Bin Liu Junyi Gai Dongqing Xu National Center for Soybean Improvement State Key Laboratory of Crop Genetics&Germplasm Enhancement and UtilizationCollege of AgricultureNanjing Agricultural UniversityNanjing 210095China Beijing Key Laboratory of Environmentally Friendly Management of Fruit Diseases and Pests in North China Institute of Plant and Environment ProtectionBeijing Academy of Agriculture and Forestry SciencesBeijing 100097China Institute of Crop Science College of Agriculture and BiotechnologyZhejiang UniversityHangzhou 310058China State Key Laboratory of Crop Gene Resources and Breeding Institute of Crop ScienceChinese Academy of Agricultural SciencesBeijing 100081China

Isoflavonoids,secondary metabolites derived from the phenylalanine pathway,are predominantly bio-synthesized in legumes,especially soybean(Glycine max).They are not only essential for plant responses to biotic and abiotic stresses but also beneficial to human *** this study,we report that light signaling controls isoflavonoid biosynthesis in ***-light photoreceptors(GmCRY1s,GmCRY2s,GmPHOT1s,and GmPHOT2s)and the transcription factors GmSTF1 and GmSTF2 promote isoflavonoid accumulation,whereas the E3 ubiquitin ligase GmCOP1b negatively regulates isoflavonoid ***1s and GmPHOT2s stabilize GmSTF1/2,whereas GmCOP1b promotes the degradation of these two proteins in ***1/2 regulate the expression of approximately 27.9%of the genes involved in soybean isoflavonoid biosynthesis,including GmPAL2.1,GmPAL2.3,and *** also repress the expression of GmBBX4,a negative regulator of isoflavonoid biosynthesis in *** addition,GmBBX4 physically interacts with GmSTF1 and GmSTF2 to inhibit their transcriptional activation activity toward target genes related to isoflavonoid ***,GmSTF1/2 and GmBBX4 form a negative feedback loop that acts downstream of photoreceptors in the regulation of isoflavonoid *** study provides novel insights into the control of isoflavonoid biosynthesis by light signaling in soybean and will contribute to the breeding of soybean cultivars with high isoflavonoid content through genetic and metabolic engineering.

关键词： photoreceptor light signaling isoflavonoid GmSTF GmBBX4 soybean

Genomic Features and Regulatory Roles of Intermediate-Sized Non-Coding RNAs in Arabidopsis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Molecular Plant 2014年第3期7卷 514-527页

作者： Yuqiu wang xuncheng wang Wei Deng Xiuduo Fan Ting-Ting Liu Guangming He Runsheng Chen William Terzaghi Danmeng Zhu Xing wang Deng College of Life Sciences Beijing Normal University Beijing 100875 China Peking-Yale Joint ResearchCenter for Plant Molecular Genetics and Agrobiotechnology National Laboratory of Protein and Plant Gene ResearchCollege of Life Sciences Peking University Beijing 100871 China Tsinghua-Peking Joint Center for Life Sciences School of Life Sciences Tsinghua University Beijing 100084 China Bioinformatics Laboratory and National Laboratory of Biomacromolecules -Institute of Biophysics. Beijing 100101 China Department of Molecular Cellular and Developmental Biology Yale University New Haven CT 06520 USA Department of Biology Wilkes University Wilkes-Barre PA 1B766 USA

ABSTRACT Recent advances in genome-wide techniques allowed the identification of thousands of non-coding RNAs with various sizes in eukaryotes, some of which have further been shown to serve important functions in many biologi- cal processes. However, in model plant Arabidopsis, novel intermediate-sized ncRNAs （im-ncRNAs）（50-300 nt） have very limited information. By using a modified isolation strategy combined with deep-sequencing technology, we identified 838 im-ncRNAs in Arabidopsis globally. More than half （58%） are new ncRNA species, mostly evolutionary divergent. Interestingly, annotated protein-coding genes with 5＇-UTR-derived novel im-ncRNAs tend to be highly expressed. For intergenic im-ncRNAs, their average abundances were comparable to mRNAs in seedlings, but subsets exhibited signifi- cantly lower expression in senescing leaves. Further, intergenic im-ncRNAs were regulated by similar genetic and epige- netic mechanisms to those of protein-coding genes, and some showed developmentally regulated expression patterns. Large-scale reverse genetic screening showed that the down-regulation of a number of im-ncRNAs resulted in either obvious molecular changes or abnormal developmental phenotypes in vivo, indicating the functional importance of im-ncRNAs in plant growth and development. Together, our results demonstrate that novel Arabidopsis im-ncRNAs are developmentally regulated and functional components discovered in the transcriptome.

关键词： intermediate-sized non-coding RNAs Arabidopsis RNomics.

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

杜甫授官、贬官与罢官说

天水师范学院学报 2010年第4期30卷 22-33页

作者：王勋成兰州大学文学院甘肃兰州730030

杜甫一生授官四次,贬官一次,罢官一次。杜甫第一次授官右卫率府胄曹参军是在天宝十四载十月,其授官原因是他献《三大礼赋》而被玄宗所赏,命中书门下试文取得了献赋出身之故,是由吏部注授的。至德二载四月,杜甫从安禄山叛军占领的长安城... 详细信息

杜甫一生授官四次,贬官一次,罢官一次。杜甫第一次授官右卫率府胄曹参军是在天宝十四载十月,其授官原因是他献《三大礼赋》而被玄宗所赏,命中书门下试文取得了献赋出身之故,是由吏部注授的。至德二载四月,杜甫从安禄山叛军占领的长安城逃至肃宗的行在凤翔,五月,由胄曹参军提升为左拾遗,这是他第二次授官,是由中书门下敕授的。乾元元年六月,杜甫因房琯之党由左拾遗贬为华州司功参军,是中书门下以敕书形式贬谪的。乾元二年九月,杜甫因一任四考已满,便被吏部罢官停秩,开始了其流浪生活。广德元年十二月,杜甫在梓州,由严武举荐,被中书门下敕授为京兆府功曹参军,但他却未赴任。这是他第三次授官。广德二年夏秋之交,杜甫在成都被严武聘为剑南东西川节度参谋。十二月,又因严武举荐被中书门下制授为检校工部员外郎、赐绯鱼袋,这是他第四次授官。从此,他就以工部员外郎为其前资官漂泊而终。

关键词：授官贬官罢官选举制

在线全文

学校读者我要写书评

暂无评论

安徽铜陵及邻区早石炭世沉积古地理

古地理学报 2010年第4期12卷 371-383页

作者：叶春林刘家润王训诚蒋少涌南京大学地球科学与工程学院内生金属矿床成矿机制研究国家重点实验室江苏南京210093 安徽省地质矿产勘探局321地质队安徽铜陵244033

依据前人研究结果及实测剖面资料、基于层序地层所获得的沉积古地理的研究结果表明:以安徽铜陵为中心区域的研究区,在早石炭世沉积层序CSQ1沉积时期存在呈北东—南西向延展、包含繁昌—铜陵—贵池在内的剥蚀区——繁贵岛,自CSQ2沉积晚... 详细信息

依据前人研究结果及实测剖面资料、基于层序地层所获得的沉积古地理的研究结果表明:以安徽铜陵为中心区域的研究区,在早石炭世沉积层序CSQ1沉积时期存在呈北东—南西向延展、包含繁昌—铜陵—贵池在内的剥蚀区——繁贵岛,自CSQ2沉积晚期该岛被海侵海泛分割为铜陵北东的繁岛和南西的贵岛两部分,并存续至CSQ3沉积末期;繁贵岛和繁岛、贵岛两侧以及繁岛与贵岛之间为海相、滨海平原相沉积区,发育有浅海相碳酸盐、混有陆源碎屑的碳酸盐以及滨浅海和滨海平原陆源碎屑沉积,各沉积带主要呈北东—南西向展布。此外在CSQ3沉积晚期还发育有潮坪碳酸盐沉积。随着海平面变化,沉积区扩大,岛区缩小,沉积带类型及其空间位置发生变化和迁移摆动。沉积古地理的变化表明,在CSQ1和CSQ3两沉积时期分别发育同沉积断裂(带),后一期断裂横穿原繁贵岛而导致铜陵地区新生海区形成,构成可能的海底热水喷流沉积成矿的构造与古地理背景。

关键词：安徽铜陵早石炭世沉积古地理同沉积断裂层序地层

在线全文

学校读者我要写书评

暂无评论

防治葡萄溃疡病的杀菌剂筛选与评价

植物保护 2023年第3期49卷 323-327,337页

作者：刘梅金添王慧李永华王训成李兴红燕继晔北京市农林科学院植物保护研究所北方果树病虫害绿色防控北京市重点实验室北京100097

采用菌丝生长速率法和孢子萌发法测定了7种杀菌剂对可可毛色二孢CSS-01s的室内生物活性,筛选出3种抑制效果较好的杀菌剂;通过对离体葡萄绿枝条及盆栽葡萄幼苗新梢进行人工接种病原菌,进一步评价杀菌剂的防治效果。结果表明,7种杀菌剂对... 详细信息

采用菌丝生长速率法和孢子萌发法测定了7种杀菌剂对可可毛色二孢CSS-01s的室内生物活性,筛选出3种抑制效果较好的杀菌剂;通过对离体葡萄绿枝条及盆栽葡萄幼苗新梢进行人工接种病原菌,进一步评价杀菌剂的防治效果。结果表明,7种杀菌剂对可可毛色二孢菌丝生长和孢子萌发表现出不同的抑制活性,其中戊唑醇、氯氟醚菌唑、啶酰菌胺和氟啶胺对菌丝生长的抑制作用较强,EC_(50)分别为0.116、0.137、0.109μg/mL和0.119μg/mL;戊唑醇、氯氟醚菌唑、氟啶胺对孢子萌发的抑制作用较强,EC_(50)分别为0.420、0.595μg/mL和1.885μg/mL。CSS-01s接种离体葡萄绿枝条试验中,戊唑醇100、200 mg/L,氯氟醚菌唑100、300 mg/L和氟啶胺100、200 mg/L的防治效果无显著差异,在57.81%~65.31%;CSS-01s接种葡萄幼苗新梢试验中,氟啶胺200 mg/L的防治效果最好,为77.56%,与氟啶胺100 mg/L、氯氟醚菌唑100、300 mg/L和戊唑醇200 mg/L之间无显著差异,但显著高于戊唑醇100 mg/L。研究结果显示,氯氟醚菌唑、戊唑醇和氟啶胺可以作为推荐杀菌剂,经进一步的田间药效评价用于葡萄溃疡病的防治。

关键词：葡萄溃疡病可可毛色二孢杀菌剂室内生物活性防治效果