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Study on the adsorptive catalytic voltammetry of aloe-emodin at a carbon paste electrode

Science China Chemistry 2005年第5期48卷 442-448页

作者： LI Ju'nan GAO Peng LI Xiangling YAN Zhihong mao xun College of Chemistry Xiangtan University Xiangtan 411105 China College of Chemistry Xiangtan University Xiangtan 411105 China College of Chemistry Xiangtan University Xiangtan 411105 China College of Chemistry Xiangtan University Xiangtan 411105 China College of Chemistry Xiangtan University Xiangtan 411105 China

A new catalytic voltammetric method for the determination of anthraqunone medi-cines at a carbon paste electrode (CPE) was described for the first time. The mechanism of the catalytic reaction was investigated by using linear sweep voltammetry, cyclic voltammetry, con-stant potential electrolysis and so on. The experiment results indicate that aloe-emodin was effi-ciently accumulated at a CPE by adsorption. In the following potential scan, aloe-emodin was reduced to homologous anthrahydroquinone compound, then the compound was immediately oxidized to aloe-emodin by the dissolved oxygen, and the aloe-emodin was again reduced at the CPE. As a result, a cyclic catalytic reaction was established. But a reversible redox reaction of aloe-emodin can only be observed at a mercury electrode, no catalytic reaction occurs there. A sensitive catalytic voltammetric peak of aloe-emodin was obtained at about ?0.60 V (vs. SCE) in 0.56 mol/L NH3-NH4Cl buffer (pH 8.9). The proposed method was applied to the determination of aloe-emodin in the Radix Rhei with satisfactory results. The determination results were in good agreement with reference values obtained by the HPLC. The adsorptive catalytic voltammetry for the determination of organic compound at CPE, chemically modified electrode and other solid electrodes could be significant in the studies on pharmacology, pharmacodynamics, toxicity of medicine, clinical medicine and biochemistry.

关键词： aloe-emodin, adsorptive catalytic voltammetry, carbon paste electrode.

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Electrochemical detection of human ferritin based on gold nanorod reporter probe and cotton thread immunoassay device

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Chinese Chemical Letters 2017年第2期28卷 226-230页

作者： Ting-Ting Song Wei Wang Li-Li Meng Yan Liu Xiao-Bo Jia xun mao Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education College of Chemistry & Materials Science Northwest University Xi'an 710127 China Center of Analysis Guangdong Medical College Dongguan 523808 China

In this study,a natural cotton thread immunoassay device combined with gold nanorod（GNR） reporter probe is developed for the rapid,sensitive and quantitative electrochemical determination of human ferritin,a lung cancer related *** ferritin as an analyte and a pair of monoclonal antibodies are used to demonstrate the proof-of-concept on the cotton thread immunoassay *** enhancement of the sensitivity is achieved by using gold nanorod as an electroactive report probe compared with a traditional gold nanoparticle（GNP） report *** device was capable of measuring 1.58 ng/mL ferritin in 30 min by anodic stripping voltammetry（ASV） testing,which meet the requirement for clinical diagnosis.

关键词： Cotton thread immunoassay device Gold nanorod reporter probe Point-of-care diagnosis Protein detection Electrochemical immunoassay

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LIN28A attenuates high glucose-induced retinal pigmented epithelium injury through activating SIRT1-dependent autophagy

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International Journal of Ophthalmology(English edition) 2023年第9期16卷 1465-1474页

作者： Dan-Qing Yu Song-Ping Yu Jing Wu Li-Na Lan Bang-xun mao Department of Endocrinology Affiliated Lishui Hospital of Zhejiang UniversityLishui 323000Zhejiang ProvinceChina Department of Ophthalmology Affiliated Lishui Hospital of Zhejiang UniversityLishui 323000Zhejiang ProvinceChina

AIM:To evaluate the effects of LIN28A(human)on high glucose-induced retinal pigmented epithelium(RPE)cell injury and its possible ***:Diabetic retinopathy model was generated following 48h of exposure to 30 mmol/L high glucose(HG)in ARPE-19 *** real-time polymerase chain reaction(qRT-PCR)and Western blot tested the expression of the corresponding genes and *** viability as well as apoptosis was determined through cell counting kit-8(CCK-8)and flow cytometry *** assay was adopted to evaluate autophagy *** 3 activity,oxidative stress markers,and cytokines were appraised adopting their commercial kits,***,ARPE-19 cells were preincubated with EX527,a Sirtuin 1(SIRT1)inhibitor,prior to HG stimulation to validate the regulatory ***:LIN28A was downregulated in HG-challenged ARPE-19 ***28A overexpression greatly inhibited HGinduced ARPE-19 cell viability loss,apoptosis,oxidative damage as well as inflammatory ***,the repressed autophagy and SIRT1 in ARPE-19 cells challenged with HG were elevated after LIN28A *** addition,treatment of EX527 greatly inhibited the activated autophagy following LIN28A overexpression and partly abolished the protective role of LIN28A against HG-elicited apoptosis,oxidative damage as well as inflammation in ARPE-19 ***:LIN28A exerts a protective role against HG-elicited RPE oxidative damage,inflammation,as well as apoptosis via regulating SIRT1/autophagy.

关键词： LIN28A retinal pigmented epithelial cells high glucose Sirtuin 1 autophagy oxidative stress

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Prognostic factors of trans-ethmosphenoid optic canal decompression for indirect traumatic optic neuropathy

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International Journal of Ophthalmology(English edition) 2018年第7期11卷 1222-1226页

作者： Ying-Jie Ma Bo Yu Yun-Hai Tu Bang-xun mao Xin-Yi Yu Wen-Can Wu Eye Hospital of Wenzhou Medical University Wenzhou 325000 Zhejiang Province China Department of Orbital and Oculoplastic Surgery Eye Hospital of Wenzhou Medical University Wenzhou 325000 Zhejiang Province China

AIM： To investigate a possible correlation between visual acuity（VA） prognosis and the presence at baseline of various orbital and ocular signs in patients affected by indirect traumatic optic neuropathy（ITON）. METHODS： From July 1 st, 2012 to July 1 st, 2015, 224 adults diagnosed with ITON who underwent endoscopic transethmosphenoid optic canal decompression（ETOCD） were reviewed. Visual outcome before and after treatment were taken into comparison. RESULTS： Accompanied older in age, longer time to medical treatment and existence of optic canal fracture（OCF） were the independent predictors for poor postoperative VA and lower improvement degree of visual acuity（IDVA）, while worse preoperative VA was predictive factor for poor postoperative VA only. Mean value of IDVA in patients with OCF was 0.19±0.30. Mean value of IDVA in patients without OCF was 0.29±0.35. IDVA in cases without OCF was significant higher than those with OCF（t=2.272, P〈0.05）. CONCLUSION： Patients suffered from ITON without OCF before ETOCD have better surgical outcome than those with OCF. Older in age, longer time to medical treatment and existence of OCF are independent factors for poor VA prognosis and lower IDVA. Preoperative VA is independent factor for VA prognosis only.

关键词： trans-ethmosphenoid optic canal decompression indirect traumatic optic neuropathy adults visual acuity improvement degree of visual acuity

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Endoscopic dacryocystorhinostomy in acute dacryocystitis:a multicenter study in China

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International Journal of Ophthalmology(English edition) 2024年第9期17卷 1689-1695页

作者： Bang-xun mao Yao-Hua Wang Zhen-Kai Liu Ning-Yu An Hai-Li Jin Ming-Ling Wang Wen-Can Wu Bo Yu Department of Ophthalmology Central Hospital of LishuiThe Fifth Affiliated Hospital of Wenzhou Medical UniversityLishui 323000Zhejiang ProvinceChina Department of Orbital Disease and Ophthalmic Tumor Affiliated Eye Hospital of Nanchang UniversityNanchang 330000Jiangxi ProvinceChina Department of Ophthalmology Renmin Hospital of ShiyanShiyan 442000Hubei ProvinceChina Eye Hospital of Ningxia Yinchuan 750000Ningxia Hui Autonomous RegionChina Eye Hospital of Wuhu City Wuhu 241000Anhui ProvinceChina Department of Ophthalmology Baoan Central Hospital of ShenzhenShenzhen 518000Guangdong ProvinceChina Department of Orbital and Oculoplastic Surgery Eye Hospital of Wenzhou Medical UniversityWenzhou 325000Zhejiang ProvinceChina

AIM:To repor t the clinical profile,endoscopic dacryocystorhinostomy(En-DCR)management,and acute dacryocystitis(AD)outcomes in ***:Clinical data of 554 adult AD patients(554 eyes)who presented in 7 tertiary eye care centers for 10y from Jan 2010 to Mar 2020 were retrospectively *** profile,En-DCR management,and outcomes of all cases were *** anatomical and functional success were evaluated for 12mo ***:The analysis included 149 males and 368 females with a median age of 55.2y(range:18-84y).There were 459 eyes with a history of epiphora or purulent *** time between a symptom of lacrimal duct obstruction and acute onset was 1 to 540(66.1±58.2)***-nine eyes had a history of the previous acute ***-four eyes developed a cutaneous fistula,while 11 eyes had post septal cellulitis ***-DCR with an anatomical success of 91.7%and functional success of 90.1%.The success rate of the patients with a history of acute episodes and the preoperative fistula was lower than the overall success ***:En-DCR can be performed during an acute episode in AD with a success rate of over 90%.

关键词： acute dacryocystitis endoscopic dacryocystorhinostomy lacrimal sac

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白术莪术提取物对小鼠急性肝损伤的保护作用

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中国老年学杂志 2013年第13期33卷 3128-3129页

作者：毛讯漯河医学高等专科学校河南漯河462000

目的研究白术莪术提取物对四氯化碳(CCl4)诱导的小鼠急性肝损伤的影响。方法给予小鼠白术莪术提取物灌胃,连续7 d,腹腔注射CCl4-花生油溶液造模急性肝损伤,16 h后测定血清丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)含量、肝组织... 详细信息

目的研究白术莪术提取物对四氯化碳(CCl4)诱导的小鼠急性肝损伤的影响。方法给予小鼠白术莪术提取物灌胃,连续7 d,腹腔注射CCl4-花生油溶液造模急性肝损伤,16 h后测定血清丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)含量、肝组织中超氧化物歧化酶(SOD)活性及其肝脏、脾脏指数。结果白术莪术提取物能显著降低急性肝损伤小鼠血清中ALT、AST含量及提高肝组织中SOD活性,减小肝脏指数(P<0.01)。结论白术莪术提取物对CCl4诱导的小鼠急性肝损伤具有很好的保护作用。

关键词：白术莪术提取物四氯化碳肝损伤

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我国网络游戏产业结构变化和企业行为选择——基于产业价值链竞争部位价值弹性的视角

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当代财经 2007年第8期 98-102页

作者：茆训诚上海师范大学金融学院上海200234

目前我国网络游戏产业虽然发展迅速,但其价值核心与技术高端部分却由国外尤其是韩国企业所掌控。本文通过引入"产业价值链竞争部位价值弹性"这一概念,考察了我国网络游戏产业的现状,同时就网络游戏企业在网络游戏这个产业链中各个具有... 详细信息

目前我国网络游戏产业虽然发展迅速,但其价值核心与技术高端部分却由国外尤其是韩国企业所掌控。本文通过引入"产业价值链竞争部位价值弹性"这一概念,考察了我国网络游戏产业的现状,同时就网络游戏企业在网络游戏这个产业链中各个具有不同价值弹性的竞争部位之间如何进行前瞻性和主动型地选择,寻求适合自身发展的竞争部位,从而在自身取得利润的同时也能够促进整个民族网络游戏产业的结构调整和发展等问题作了分析,并在此基础上对今后中国网络游戏产业的发展提出了相关预测和建议。

关键词：网络游戏产业产业价值链竞争部位价值弹性

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大孔树脂AB-8纯化麦冬多糖工艺的研究

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安徽农业科学 2010年第14期38卷 7308-7308,7338页

作者：毛讯郑州大学基础医学院河南郑州450000 漯河医学高等专科学校河南漯河462000

[目的]研究大孔树脂纯化分离麦冬多糖的优化工艺条件。[方法]比较了不同pH值、流速对麦冬多糖的纯化效果。[结果]确定大孔树脂纯化麦冬多糖的层析条件为:上柱溶液pH值为8,洗脱溶液pH值为8,流速为1.0ml/min,在此条件下麦冬多糖纯度可达到... 详细信息

[目的]研究大孔树脂纯化分离麦冬多糖的优化工艺条件。[方法]比较了不同pH值、流速对麦冬多糖的纯化效果。[结果]确定大孔树脂纯化麦冬多糖的层析条件为:上柱溶液pH值为8,洗脱溶液pH值为8,流速为1.0ml/min,在此条件下麦冬多糖纯度可达到81.0%,回收率71.2%。[结论]该优化工艺条件可以作为麦冬多糖的纯化方式进行推广。

关键词：麦冬多糖大孔树脂 AB-8

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对21世纪初广西高等教育发展的思考

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广西民族大学学报（哲学社会科学版） 2002年第z1期24卷 199-202页

作者：何茂勋广西师范大学

新中国成立以来 ,广西高等教育有了长足发展 ,但也存在不少问题。广西高等教育必须在高校布局、专业设置、师资建设、教育大众化、教育投入、教育质量上狠下工夫 ,才能适应 2

关键词：广西高等教育发展战略

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关于中国足球彩票发展的对策研究

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体育科学 2006年第1期26卷 18-30,48页

作者：李刚茆训诚复旦大学经济学院世界经济系上海200433 上海师范大学欧洲商务与文化学院上海200234

对中国足球彩票“胜负彩”各期各个地区销量、中奖情况和各奖级奖金等相关数据进行了定量回归分析,得出如下结论:足球彩票和其他彩票属性有所不同;足球彩票有着一定的智力性,了解足球运动可以适当提高中奖机率,但足球彩票不是一种投资品... 详细信息

对中国足球彩票“胜负彩”各期各个地区销量、中奖情况和各奖级奖金等相关数据进行了定量回归分析,得出如下结论:足球彩票和其他彩票属性有所不同;足球彩票有着一定的智力性,了解足球运动可以适当提高中奖机率,但足球彩票不是一种投资品;足球彩票销量总体上呈下降趋势;中大奖和娱乐是人们购买足球彩票的两个主要原因;不同地区人们购买足球彩票的心态有所不同;外界环境变化对足球彩票销量有着重大影响。在此基础上,对今后中国足球彩票的发展提出了相关的建议。

关键词：足球彩票发展对策中国

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