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Impact of crosslinking/riboflavin-UVA-photodynamic inactivation on viability,apoptosis and activation of human keratocytes in vitro

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The Journal of Biomedical Research 2015年第4期29卷 321-325页

作者： Tanja Stachon Jiong Wang xufei song Achim Langenbucher Berthold Seitz Nóra Szentmáry Department of Ophthalmology Saarland University Medical Center Department of Ophthalmology Renmin Hospital of Wuhan University Department of Ophthalmology The Second Hospital of Jilin University Experimental Ophthalmology Saarland University

Riboflavin-UVA photodynamic inactivation is a potential treatment altemative in therapy resistant infectious keratitis. The purpose of our study was to determine the impact of riboflavin-UVA photodynamic inactivation on viability, apop- tosis and activation of human keratocytes in vitro. Primary human keratocytes were isolated from human corneal buttons and cultured in DMEM/Ham＇s F12 medium supplemented with 10% fetal calf serum. Keratocytes underwent UVA light illumination （375 nm） for 4.10 minutes （2 J/cm2） during exposure to different concentrations of riboflavin. Twenty-four hours after treatment, cell viability was evaluated photometrically, whereas apoptosis, CD34 and alpha-smooth muscle actin （α-SMA） expression were assessed using flow cytometry. We did not detect significant changes in cell viability, apoptosis, CD34 and α-SMA expression in groups only treated with riboflavin or UVA light. In the group treated with riboflavin-UVA-photodynamic inactivation, viability of keratocytes decreased significantly at 0.1% riboflavin （P〈0.01） while the percentage of CD34 （P〈0.01 for both 0.05% and 0.1% riboflavin） and alpha-SMA positive keratocytes （P〈0.01 and P〈0.05 for 0.05% and 0.1% riboflavin, respectively） increased significantly compared to the controls. There was no significant change in the percentage of apoptotic keratocytes compared to controls at any of the used ribo- flavin concentrations （P=0.09 and P=0.13）. We concluded that riboflavin-UVA-photodynamic-inactivation decreases viability of myofibroblastic transformation and multipotent haematopoietic stem cell transformation; however, it does not have an impact on apoptosis of human keratocytes in vitro.

关键词： crosslinking human keratocytes viability apoptosis a-SMA CD34

Self-consistent and precise measurement of time-dependent radiative albedo of gold based on specially symmetrical triple-cavity Hohlraum

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Matter and Radiation at Extremes 2024年第3期9卷 57-64页

作者： Zhiyu Zhang Yang Zhao Xiaoying Han Liling Li Bo Qing Lifei Hou Yulong Li YuXue Zhang Huan Zhang Xiangming Liu Bo Deng Gang Xiong Min Lv Tuo Zhu Chengwu Huang Tianming song Yan Zhao Yingjie Li Lu Zhang xufei Xie Jiyan Zhang Jiamin Yang Laser Fusion Research Center China Academy of Engineering PhysicsMianyang 621900China National Key Laboratory of Plasma Physcis Mianyang 621900China Institute of Applied Physics and Computational Mathematics Beijing 100088China

A self-consistent and precise method to determine the time-dependent radiative albedo,i.e.,the ratio of the reemission flux to the incident flux,for an indirect-drive inertial confinement fusion Hohlraum wall material is proposed.A specially designed symmetrical triple-cavity gold Hohlraum is used to create approximately constant and near-equilibrium uniform radiation with a peak temperature of 160 *** incident flux at the secondary cavity waist is obtained from flux balance analysis and from the shock velocity of a standard *** results agree well owing to the symmetrical radiation in the secondary cavity.A self-consistent and precise time-dependent radiative albedo is deduced from the reliable reemission flux and the incident flux,and the result from the shock velocity is found to have a smaller uncertainty than that from the multi-angle flux balance analysis,and also to agree well with the result of a simulation using the HYADES opacity.

关键词： precise cavity radiative

腹腔镜手术戳孔出血调查统计及相关危险因素分析

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中华腔镜外科杂志（电子版） 2015年第5期8卷 35-38页

作者：董永红宋旭飞徐钧山西省人民医院普通外科一病区太原030012 山西医科大学

目的对腹腔镜手术戳孔出血进行调查分析,探讨腹腔镜手术戳孔出血的危险因素。方法对山西省人民医院2014年1月至2015年1月符合条件的腹腔镜手术944例患者进行研究,根据相关文献及临床经验选取6个相关因素进行Logistic分析,探讨腹腔镜手... 详细信息

目的对腹腔镜手术戳孔出血进行调查分析,探讨腹腔镜手术戳孔出血的危险因素。方法对山西省人民医院2014年1月至2015年1月符合条件的腹腔镜手术944例患者进行研究,根据相关文献及临床经验选取6个相关因素进行Logistic分析,探讨腹腔镜手术戳孔出血的相关危险因素。结果 944例患者中,发生戳孔出血15例,发生率为1.59%。导致腹腔镜手术戳孔出血的危险因素有年龄、体重指数、穿刺时是否采用透光法(P值分别为0.025、0.000、0.001)。结论年龄、体重指数、穿刺时是否采用透光法与腹腔镜手术戳孔出血密切相关,临床上需要重视相关危险因素,避免戳孔出血的发生。

关键词：腹腔镜手术戳孔出血危险因素

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重组人肝细胞生长因子真核表达载体的构建

中国药物与临床 2018年第2期18卷 160-162页

作者：宋旭飞董永红徐钧沈文涛周鹏山西省人民医院普外科太原030012 河南省安阳市人民医院山西医科大学第二医院普外科中国热带农业科学研究院生物技术研究所

目的构建重组人肝细胞生长因子(rhHGF)的真核表达载体,为后续毕赤酵母蛋白高效表达提供基础。方法根据酵母表达偏爱密码子合成rhHGFcDNA全长序列,将rhHGF和pGAPZαA质粒双酶切后行连接转化,构建穿梭载体pGAPZαA-rhHGF,并鉴定。结果重... 详细信息

目的构建重组人肝细胞生长因子(rhHGF)的真核表达载体,为后续毕赤酵母蛋白高效表达提供基础。方法根据酵母表达偏爱密码子合成rhHGFcDNA全长序列,将rhHGF和pGAPZαA质粒双酶切后行连接转化,构建穿梭载体pGAPZαA-rhHGF,并鉴定。结果重组真核表达载体pGAPZA-rhHGF经限制性内切酶分析,与理论值相符,测序结果未见碱基变异。结论成功构建了pGAPZαA-rhHGF真核表达载体,为后续毕赤酵母高效表达rhHGF蛋白提供了可行性。

关键词：肝细胞生长因子酵母巴斯德毕赤表达

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截止节流阀两相流致噪声特性分析

机床与液压 2024年第5期52卷 199-206页

作者：王旭飞王均宇戴磊宋永兴于跃平山东海筑暖通工程有限公司山东济南250014 山东建筑大学热能工程学院山东济南250101 压缩机技术国家重点实验室(压缩机技术安徽省实验室) 合肥安徽230031

随着空调技术的发展,新型节流阀如电子膨胀阀、截止节流阀、短管节流阀等代替了毛细管在空调上的应用。但目前对截止节流阀的研究少之甚少,故而对不同流量下截止节流阀的流场进行计算。通过数值计算得到不同工况下的压力场、速度场、声... 详细信息

随着空调技术的发展,新型节流阀如电子膨胀阀、截止节流阀、短管节流阀等代替了毛细管在空调上的应用。但目前对截止节流阀的研究少之甚少,故而对不同流量下截止节流阀的流场进行计算。通过数值计算得到不同工况下的压力场、速度场、声功率级等的分布。结果表明:随着阀门入口流量的增大,阀门节流孔处的最大流速从44.331 3 m/s增大到77.560 4 m/s;随着阀门入口流量的增大,节流孔处的压降也逐渐增大;当阀门入口流量从0.014 kg/s增大到0.024 kg/s,阀门下游最大噪声从104.640 2 dB增大到122.751 5 dB;流场中噪声主要集中在阀门下游和节流孔,且最大噪声出现在阀门下游的直管段末端,噪声的分布特点也为阀门的进一步优化提供方向。

关键词：截止节流阀数值计算流致噪声

生成式人工智能对临床实践指南制订、评价和应用的影响

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协和医学杂志 2024年第5期15卷 1173-1181页

作者：罗旭飞吕晗宋再伟刘辉王郅翔李昊东王晔朱迪张璐陈耀龙兰州大学基础医学院循证医学中心兰州730000 中国医学科学院循证评价与指南研究创新单元兰州大学基础医学院兰州730000 甘肃省循证医学重点实验室兰州730000 兰州大学健康数据科学研究院兰州730000 首都医科大学附属北京友谊医院放射科北京100050 北京大学第三医院药学部北京100191 首都医科大学附属北京友谊医院超声科北京100050 首都医科大学附属北京友谊医院精准与智慧影像实验室北京100050 兰州大学公共卫生学院兰州730000 香港浸会大学计算机科学系中国香港999077 中华医学会杂志社指南与标准研究中心兰州730000 世界卫生组织指南实施与知识转化合作中心兰州730000

生成式人工智能(generative artificial intelligence,GAI)是指能够从训练数据生成全新内容(如文本、图像或音频等)的人工智能技术。GAI工具不仅在系统评价的文献筛选、数据提取和文献评价等方面显示出快速、高效的潜力,还可用于指南的... 详细信息

生成式人工智能(generative artificial intelligence,GAI)是指能够从训练数据生成全新内容(如文本、图像或音频等)的人工智能技术。GAI工具不仅在系统评价的文献筛选、数据提取和文献评价等方面显示出快速、高效的潜力,还可用于指南的评价和传播,提高指南的可读性和推广效率。但GAI工具生成内容的准确性、相关引证证据的合理性、证据等级以及数据的可靠性仍需验证,且数据的隐私保护和伦理问题也是面临的挑战。本文旨在概述GAI工具在指南制订、评价、传播与实施中的应用现状,探索GAI工具在指南领域应用的可行性和新模式,以期提高指南的制订效率和质量,更好地服务指南制订者和使用者。

关键词：临床实践指南生成式人工智能系统评价 ChatGPT 大语言模型

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Compositional Variability and Mutation Spectra of Monophyletic SARS-CoV-2 Clades

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Genomics, Proteomics & Bioinformatics 2020年第6期18卷 648-663页

作者： xufei Teng Qianpeng Li Zhao Li Yuansheng Zhang Guangyi Niu Jingfa Xiao Jun Yu Zhang Zhang Shuhui song China National Center for Bioinformation Beijing 100101China National Genomics Data Center&CAS Key Laboratory of Genome Sciences and Information Beijing Institute of GenomicsChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China

COVID-19 and its causative pathogen SARS-CoV-2 have rushed the world into a staggering pandemic in a few months,and a global fight against both has been ***,we describe an analysis procedure where genome composition and its variables are related,through the genetic code to molecular mechanisms,based on understanding of RNA replication and its feedback loop from mutation to viral proteome sequence fraternity including effective sites on the replicase-transcriptase *** analysis starts with primary sequence information,identity-based phylogeny based on 22,051 SARS-CoV-2 sequences,and evaluation of sequence variation patterns as mutation spectra and its 12 permutations among organized *** are tailored to two key mechanisms:strand-biased and function-associated *** findings are listed as follows:1)The most dominant mutation is C-to-U permutation,whose abundant second-codon-position counts alter amino acid composition toward higher molecular weight and lower hydrophobicity,albeit assumed most slightly deleterious.2)The second abundance group includes three negative-strand mutations(U-to-C,A-to-G,and G-to-A)and a positive-strand mutation(G-to-U)due to DNA repair mechanisms after cellular abasic events.3)A clade-associated biased mutation trend is found attributable to elevated level of negative-sense strand synthesis.4)Within-clade permutation variation is very informative for associating non-synonymous mutations and viral proteome *** findings demand a platform where emerging mutations are mapped onto mostly subtle but fast-adjusting viral proteomes and transcriptomes,to provide biological and clinical information after logical convergence for effective pharmaceutical and diagnostic *** actions are in desperate need,especially in the middle of the War against COVID-19.

关键词： SARS-CoV-2 Nucleotide composition Mutation spectrum Viral replication

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煤矿采空区积水瞬变电磁法探测方法优化

煤矿安全 2024年第9期55卷 189-196页

作者：崔建廷宋贵磊白武公绪飞于凤海白小军李光柱马丁鄂尔多斯市昊华精煤有限责任公司山东科技大学能源与矿业工程学院

应用矿井瞬变电磁法进行含水异常体的探测效果明显,但容易受到巷道复杂工程地质条件和设备环境的干扰,需要进行针对性优化以提高矿井瞬变电磁法信号采集质量和解释精度。基于“烟圈”扩散理论,阐述了矿井瞬变电磁法探测原理及视电阻率... 详细信息

应用矿井瞬变电磁法进行含水异常体的探测效果明显,但容易受到巷道复杂工程地质条件和设备环境的干扰,需要进行针对性优化以提高矿井瞬变电磁法信号采集质量和解释精度。基于“烟圈”扩散理论,阐述了矿井瞬变电磁法探测原理及视电阻率纵向微分解释方法;通过对已知积水采空区进行瞬变电磁法探测试验,分析对比了主要采集参数对瞬变电磁法电场响应特征的影响;结合视电阻率纵向微分解释方法,提出了矿井瞬变电磁法采空区积水探测的数据采集与处理优化方案并对其进行了效果评价。结果表明:线圈与巷道围岩之间距离越近,两者耦合程度越高,且该距离存在合理范围,超出该范围线圈接收到的电压响应值过低,不满足数据对信噪比的要求;增加叠加次数可提高信噪比,并有效压制电压衰减曲线尾支畸变;适用于高家梁煤矿的合理距离范围为0～0.5 m,叠加次数为64次。

关键词：矿井瞬变电磁法视电阻率微分数据采集参数采空区积水电性界面

Ambient volatile organic compounds at a receptor site in the Pearl River Delta region:Variations,source apportionment and effects on ozone formation

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Journal of Environmental Sciences 2022年第1期34卷 104-117页

作者： Yao Meng Junwei song Lewei Zeng Yingyi Zhang Yan Zhao xufei Liu Hai Guo Liuju Zhong Yubo Ou Yan Zhou Tao Zhang Dingli Yue Senchao Lai The Key Lab of Pollution Control and Ecosystem Restoration in Industry Clusters Ministry of Educationand Guangdong Provincial Key Laboratory of Atmospheric Environment and Pollution ControlSchool of Environment and EnergySouth China University of TechnologyGuangzhou 510006China Institute of Meteorology and Climate Research Karlsruhe Institute of TechnologyHermann-von-Helmholtz-Platz 1Eggenstein-Leopoldshafen 76344Germany Department of Civil and Environmental Engineering The Hong Kong Polytechnic UniversityHong KongChina Guangdong Environmental Monitoring Center State Environmental Protection Key Laboratory of Regional Air Quality MonitoringGuangzhou 510308China Guangdong Polytechnic of Environmental Protection Engineering Foshan 528216China

We present the continuously measurements of volatile organic compounds(VOCs)at a receptor site(Wan Qing Sha,WQS)in the Pearl River Delta(PRD)region from September to November of *** average mixing ratios of total VOCs(TVOCs)was 36.3±27.9 ppbv with the dominant contribution from alkanes(55.5%),followed by aromatics(33.3%).The diurnal variation of TVOCs showed a strong photochemical consumption during daytime,resulting in the formation of ozone(O_(3)).Five VOC sources were resolved by the positive matrix factorization(PMF)model,including solvent usage(28.6%),liquid petroleum gas(LPG)usage(24.4%),vehicle exhaust(21.0%),industrial emissions(13.2%)and gasoline evaporation(12.9%).The regional transport air masses from the upwind cities of south China can result in the elevated concentrations of *** ratios of TVOCs/NO_(x)(1.53±0.88)suggested that the O_(3) formation regime at WQS site was VOC-limited,which also confirmed by a photochemical box model with the master chemical mechanism(PBM-MCM).Furthermore,the observation on high-O_(3) episode days revealed that frequent O_(3) outbreaks at WQS were mainly caused by the regional transport of anthropogenic VOCs especially for aromatics and the subsequent photochemical *** study provides valuable information for policymakers to propose the effective control strategies on photochemical pollution in a regional perspective.

关键词： Volatile organic compounds(VOCs) Pearl River Delta(PRD)region Source apportionment Ozone(O_(3)) Regional transport