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Peri-centrosomal localization of small interfering RNAs in C. elegans

Science China Life Sciences 2025年

作者： Qile Jin xuezhu feng Minjie Hong Ke Wang Xiangyang Chen Jiewei Cheng Yan Kuang Xiaoyue Si Mingjing Xu Xinya Huang Shouhong Guang Chengming Zhu Department of Obstetrics and Gynecology The First Affiliated Hospital of USTC The USTC RNA Institute Hefei National Research Center for Physical Sciences at the Microscale School of Life Sciences Division of Life Sciences and Medicine Biomedical Sciences and Health Laboratory of Anhui Province University of Science and Technology of China School of Basic Medicine Anhui Medical University

The centrosome is the microtubule-organizing center and a crucial part of cell division. Centrosomal RNAs(cnRNAs) have been reported to enable precise spatiotemporal control of gene expression during cell division in many species. Whether and how cnRNAs exist in C. elegans are unclear. Here, using the nuclear RNAi Argonaute protein NRDE-3 as a reporter, we observed potential peri-centrosome localized small interfering(si)RNAs in C. elegans. NRDE-3 was previously shown to associate with pre-mRNAs and pre-rRNAs via a process involving the presence of complementary siRNAs. We generated a GFP-NRDE-3 knock-in transgene through CRISPR/Cas9 technology and observed that NRDE-3 formed peri-centrosomal foci neighboring the tubulin protein TBB-2, other centriole proteins and pericentriolar material(PCM)components in C. elegans embryos. The peri-centrosomal accumulation of NRDE-3 depends on RNA-dependent RNA polymerase(RdRP)-synthesized 22G siRNAs and the PAZ domain of NRDE-3, which is essential for siRNA binding. Mutation of eri-1, ergo-1, or drh-3 significantly increased the percentage of pericentrosome-enriched NRDE-3. At the metaphase of the cell cycle, NRDE-3 was enriched in both the peri-centrosomal region and the spindle. Moreover, the integrity of centriole proteins and pericentriolar material(PCM) components is also required for the peri-centrosomal accumulation of NRDE-3. Therefore, we concluded that siRNAs could accumulate in the pericentrosomal region in C. elegans and suggested that the peri-centrosomal region may also be a platform for RNAi-mediated gene regulation.

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Small RNAs,RNAi and the Inheritance of Gene Silencing in Caenorhabditis elegans

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Journal of Genetics and Genomics 2013年第4期40卷 153-160页

作者： xuezhu feng Shouhong Guang School of Life Sciences University of Science and Technology of China

Invasive nucleic acids such as transposons and viruses usually exhibit aberrant characteristics,e.g.,unpaired DNA or abnormal doublestranded *** employ a variety of strategies to defend themselves by distinguishing self and nonself substances and disabling these invasive nucleic ***,they have developed ways to remember this exposure to invaders and transmit the experience to their *** mechanism underlying this inheritance has remained *** research has shed light on the initiation and maintenance of RNA-mediated inherited gene *** regulatory RNAs play a variety of crucial roles in organisms,including gene regulation,developmental timing,antiviral defense,and genome integrity,via a process termed as RNA interference（RNAi）.Recent research has revealed that small RNAs and the RNAi machinery are engaged in establishing and promoting transgenerational gene *** RNAs direct the RNAi and chromatin modification machinery to the cognate nucleic acids to regulate gene expression and epigenetic ***,these acquired small RNAs and epigenetic changes persist and are transmitted from parents to offspring for multiple ***,RNAi is a vital determinant of the inheritance of gene silencing and acts as a driving force of evolution.

关键词： RNAi Small RNA Inheritance Gene silencing nrde

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Non-coding RNAs mediate the rearrangements of genomic DNA in ciliates

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Science China(Life Sciences) 2013年第10期56卷 937-943页

作者： feng xuezhu GUANG ShouHong School of Life Sciences University of Science and Technology of China

Most eukaryotes employ a variety of mechanisms to defend the integrity of their genome by recognizing and silencing parasitic mobile nucleic ***,recent studies have shown that genomic DNA undergoes extensive rearrangements,including DNA elimination,fragmentation,and unscrambling,during the sexual reproduction of ciliated ***-coding RNAs have been identified to program and regulate genome rearrangement *** Paramecium and Tetrahymena,scan RNAs(scnRNAs)are produced from micronuclei and transported to vegetative macronuclei,in which scnRNA elicits the elimination of cognate genomic *** contrast,Piwi-interacting RNAs(piRNAs)in Oxytricha enable the retention of genomic DNA that exhibits sequence complementarity in *** RNA interference(RNAi)-like mechanism has been found to direct these genomic ***,in Oxytricha,maternal RNA templates can guide the unscrambling process of genomic *** non-coding RNA-directed genome rearrangements may have profound evolutionary implications,for example,eliciting the multigenerational inheritance of acquired adaptive traits.

关键词： RNAi gene silencing scnRNA piRNA rearrangement elimination

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The SNAPc complex mediates starvation-induced trans-splicing in Caenorhabditis elegans

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Journal of Genetics and Genomics 2022年第10期49卷 952-964页

作者： Xinhao Hou Chengming Zhu Mingjing Xu Xiangyang Chen Cheng Sun Björn Nashan Shouhong Guang xuezhu feng The First Affiliated Hospital of USTC Division of Life Sciences and MedicineSchool of Life SciencesMinistry of Education Key Laboratory for Membraneless Organelles&Cellular DynamicsThe USTC RNA InstituteDepartment of Obstetrics and GynecologyBiomedical Sciences and Health Laboratory of Anhui ProvinceUniversity of Science and Technology of ChinaHefeiAnhui 230001China CAS Center for Excellence in Molecular Cell Science Chinese Academy of SciencesHefeiAnhui 230027China

Dietary restriction usually suppresses biosynthesis but activates catabolic pathways in ***,the short-term starvation enhances biosynthetic activities and promotes ribosomal biogenesis in adult Caenorhabditis *** mechanism underlying the processes remains largely ***,we find that the short-term starvation enhances the SL1 trans-splicing of translation-related genes in adult *** by transcriptome *** small nuclear RNA-activating protein complex(SNAPc)promotes SL RNA production and mediates starvation-induced ***-5,a core factor in the upstream sequence transcription complex(USTC)essential for piRNA production,is also involved in the starvationinduced trans-splicing *** down components of the SNAPc complex and tofu-5 extends worm survival under starvation *** together,our study highlights the importance of SL transsplicing in the nutrition response and reveals a mechanism of the survival regulation by food deprivation via SNAPc and TOFU-5.

关键词： Trans-splicing SNAPc tofu-5 Starvation C.elegans

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Phase-separated liquid compartments promote efficient RNAi response in ***

Phase-separated liquid compartments promote efficient RNAi r...

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2021年全国生物相分离和相变学术研讨会

作者： Xiangyang Chen Ke Wang Shouhong Guang xuezhu feng Ministry of Education Key Laboratory for Membraneless Organelles & Cellular Dynamics Hefei National Laboratory for Physical Sciences at the MicroscaleSchool of Life SciencesDivision of Life Sciences and MedicineUniversity of Science and Technology of China

Introduction of dsRNA results in silencing of homologous genes in many *** ***,the initial RNAi response is amplified by the recruitment of RNA-dependent RNA polymerases onto target mRNAs,which dramatically enhance RNAi response via synthesizing abundant secondary *** proteins participated in small RNA amplification and RNAi response are localized in distinct germ granules,which are membraneless compartments formed via liquid-liquid phase separation.A series of distinct peri-nuclear germ granules have been identified,including P granule,Z-granule,M-granule and SIMR foci,which are spatially *** RNAs are proposed to translocate between these granules to elicit robust RNAi *** is still unclear whether and how these liquid-like condensates are spatiotemporally regulated in ***,we use functional proteomics in *** and identify an EGO complex(EGC).We identified two new germline proteins,EGC-1 and EGC-2,that are required for efficient RNAi response and inheritance of *** complex is localized in a new germ granule adjacent to P-granule,which was named *** in E-granule formation reshape the spatial assembly of multiple germ *** speculate that disorders of germ granule assembly block efficient small RNA amplification,resulting in defects in germline RNAi response and development.

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Erroneous ribosomal RNAs promote the generation of antisense ribosomal siRNA

Erroneous ribosomal RNAs promote the generation of antisense...

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第五届中国秀丽线虫大会

作者： Chengming Zhu Qi Yan xuezhu feng Shouhong Guang University of Science and Technology of China

Ribosome biogenesis is a very sophisticated multi-step process,during which mistakes could take place at any step during pre-rRNA processing,rRNA modification,and assembly of ribosomal *** rRNAs are usually detected and degraded by multiple surveillance ***,we identified a new class of antisense ribosomal siRNAs(risiRNAs) that downregulate pre-rRNA through the nuclear RNAi pathway in a suppressor of siRNA(susi)-1 mutant in ***.

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秀丽线虫细胞核内小干扰RNA调控基因表达的机制研究

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中国科学技术大学学报 2013年第11期43卷 933-940页

作者：冯雪竹光寿红中国科学技术大学生命科学学院

生物体内存在大量的不编码蛋白质序列的非编码RNA(noncoding RNA,ncRNA),这些非编码RNA广泛参与生命活动的各个过程,包括基因表达调控、基因组稳定性维持、抵抗外源核酸侵染、发育的时序调节以及肿瘤发生等.越来越多的证据表明一系列重... 详细信息

生物体内存在大量的不编码蛋白质序列的非编码RNA(noncoding RNA,ncRNA),这些非编码RNA广泛参与生命活动的各个过程,包括基因表达调控、基因组稳定性维持、抵抗外源核酸侵染、发育的时序调节以及肿瘤发生等.越来越多的证据表明一系列重大疾病的发生、发展与这些非编码RNA的产生和调控失衡相关.小调节性RNA正在成为潜在的疾病标志物、药物靶点和生物分子药物.我们的研究主要集中在高等多细胞生物中细胞核里小干扰RNA调控基因表达的分子机制和生物学功能.我们在模式生物秀丽线虫中通过遗传筛选的方法发现了一条小干扰RNA在细胞核内调控基因表达的通路,以及参与这条通路的几个关键的细胞核RNA干扰缺陷型基因(nuclear RNAi defective,Nrde).这一发现不仅解决了高等多细胞生物中细胞核内是否存在小RNA干扰现象的争论,而且发现小RNA可能通过主动转运的方式进入细胞核并调控RNA聚合酶Ⅱ(RNAPⅡ)介导的转录延伸.这一通路还可能参与了生物体的获得性遗传过程.本文重点阐述这一小干扰RNA调控基因表达的分子机制,并提出未来亟待解决的科学问题和发展方向.

关键词：非编码RNA 小干扰RNA RNA干扰转录基因表达调控细胞核RNA干扰缺陷型

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Targeted chromosomal translocations and essential gene knockout using CRISPR/Cas9 technology in ***

Targeted chromosomal translocations and essential gene knock...

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第九届全国核糖核酸学术讨论会

作者：陈向阳 Mu Li xuezhu feng Shouhong Guang 中国科学技术大学

＜正＞Many genes play essential roles in development and fertility;their disruption leads to growth arrest or *** balancers have been widely used to study essential genes in many ***,it is technically challenging and ...

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细胞核内小干扰RNA介导的表观遗传和基因表达调控

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中国科学：生命科学 2017年第1期47卷 59-68页

作者：冯雪竹光寿红中国科学技术大学生命科学学院合肥230027

RNA干扰(RNAi)是真核生物中一个非常广泛而且保守的基因表达调节机制.长度为20~30个核苷酸的具有调控作用的小RNA广泛存在于几乎所有的真核细胞中,这些小RNA参与和调控许多基本的生命过程,如异染色质的形成、基因组稳定性的维持、发育... 详细信息

RNA干扰(RNAi)是真核生物中一个非常广泛而且保守的基因表达调节机制.长度为20~30个核苷酸的具有调控作用的小RNA广泛存在于几乎所有的真核细胞中,这些小RNA参与和调控许多基本的生命过程,如异染色质的形成、基因组稳定性的维持、发育的时空调节、细胞命运的决定、DNA的损伤与修复反应,以及抗病毒反应等.这些小RNA的产生和调节的失衡往往会导致一系列重大疾病的发生与发展.RNAi在细胞质里主要是通过抑制翻译和诱导靶标mRNA的降解来进行.在细胞核中,RNAi则有多种作用方式.本文将重点阐述小RNA在细胞核里的基因表达调控的分子机制,包括诱导异染色质形成、介导表观遗传修饰、调控染色体分离,以及调控转录和选择性剪切等过程.

关键词： siRNA RNAi 转录组蛋白修饰表观遗传

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RNA-dependent RNA polymerases-synthesized antisense ribosomal siRNAs silence pre-rRNA via the nuclear RNAi pathway in ***

RNA-dependent RNA polymerases-synthesized antisense ribosoma...

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第九届全国核糖核酸学术讨论会

作者：周旭飞 xuezhu feng Mu Li Fei Xu Kai Hu Shouhong Guang 中国科学技术大学生命科学学院

The biogenesis of rRNA drives cell growth and proliferation,but mechanisms that modulate rRNA production remain poorly *** have conducted a genetic screen to look for factors that negatively regulate endo-si RNA generation in ***,and identified the suppressor of si RNA（susi）-1and a new class of triphosphorylated antisense ribosomal si RNAs（risi RNAs）.In susi-1 mutant,we observed an accumulation of si RNA sequences complementary to 18S and 26S *** RNAs are similar to 22G-RNA,as they are 22 nt in length and have a triphosphorylated guanidine at their 5’-*** generation of risi RNAs depends on RNA-dependentRNA polymerases,but not the ERI/Dicer *** RNAs act through the nuclearRNAi pathway and guide the Argonaute protein NRDE-3 to the nucleoli,which leads to a down regulation of pre-rRNA ***,low temperature also induces the accumulation of risi RNA,suggesting potential regulatory *** SUSI-1 shows homology to Dis3-like exonuclease 2（Dis3L2）in other organisms,a gene identified as a key exonuclease involved in the 3’to 5’degradation of oligouridylated *** observed an accumulation of 3’-tail oligouridylated 26S rRNA in susi-1mutant and in low temperature-treated ***,the injection of 3’-tail oligouridylated 26S rRNA elicited a nuclear accumulation of *** findings thus establish SUSI-1（ce Dis3L2）as a suppressor of endo-si RNA generation,identify a novel class of risi RNAs that suppress pre-rRNA expression,and suggest a potential disease mechanism.

关键词： Dis3L2 risiRNA NRDE nucleolus low temperature

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