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microRNA-218 promotes gemcitabine sensitivity in human pancreatic cancer cells by regulating HMGB1 expression

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Chinese Journal of Cancer Research 2015年第3期27卷 267-278页

作者： Zhe Liu Ruixia du Jin Long Kejian Guo Chunlin Ge Shulong Bi Yuanhong Xu Department of Pancreatic Surgery First Hospital of China Medical University Department of Otorhinolaryngology Fengtian HospitalShenyang Medical University

Objective： The purpose of this study was to examine the effect of gemcitabine（GEM） on micro RNA-218（mi R-218） expression in human pancreatic cancer ***： Quantitative reverse transcription polymerase chain reaction（q RT-PCR） was performed to examine the differences in mi R-218 expression between the GEM-sensitive Bx PC-3 pancreatic cancer cells and GEM-resistant PANC-1 cells. The effect of GEM on the expression of mi R-218 in PANC-1 cells was also investigated. PANC-1 cells were transfected either with HMGB1 si RNA to knock down the expression of HMGB1 or with the recombinant HMGB1 expression vector（pc DNA3.1-HMGB1） to overexpress HMGB1. The effect of ectopic expression of HMGB1 on the apoptosis of mi R-218-transfected and GEMtreated PANC-1 cells was examined by flow cytometric ***： The mi R-218 expression level was lower in GEM-resistant PANC-1 cells compared to GEMsensitive Bx PC-3 cells（P〈0.05）. The percentage of apoptotic PANC-1 cells was significantly increased in the mi R-218 mimic ＋ GEM group compared to the mimic ctrl ＋ GEM group and the normal control group（P〈0.01）. The HMGB1 expression level was markedly decreased in PANC-1 cells transfected with HMGB1 si RNA but was significantly increased in PANC-1 cells transfected with the recombinant HMGB1 expression vector, pc DNA3.1-HMGB1（P〈0.01）. The proportion of apoptotic PANC-1 cells was significantly lower in the mi R-218 mimic ＋ GEM ＋ pc DNA3.1-HMGB1 group compared to the mi R-218 mimic ＋ GEM ＋ HMGB1 si RNA group（P〈0.01）.Conclusions： The expression level of mi R-218 was downregulated in the GEM-resistant cell line. mi R-218 promoted the sensitivity of PANC-1 cells to GEM, which was achieved mainly through regulating the expression of HMGB1 in PANC-1 cells.

关键词： Pancreatic cancer microRNA-218 (miR-218) gemcitabine (GEM) apoptosis high mobility group box 1 (HMGB1)

Prevention of halfway cracks in continuous cast A36 steel slabs containing boron

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China Foundry 2015年第1期12卷 26-31页

作者： Jian-chao Ma Qing-feng Zhou du-xing Su Xu-yan Deng Research Institute of Iron and Steel Sha Steel

The formation of halfway cracks in continuous cast A36 steel slabs containing 17 ppm boron was studied by investigating the zero ductility temperature(ZDT), zero strength temperature(ZST), solidification characteristics, and strain distribution in the steels. Results show that the formation of halfway cracks in A36-B slab is attributed to the increase in both the internal crack susceptibility(ZDT and ZST) of the steel and the external tensile strain at the solidification front. The ZST of both steels with(A36-B) and without(A36) boron addition is nearly the same, but the ZDT of A36-B steel is found 50 oC lower due to a considerable increase of boron content at the final stage of solidification. The decrease of ZDT enlarges the solidification cracking susceptibility zone and results in the A36-B steel being more prone to cracking. In addition, during the unbending segments, a large tensile strain, resulting from the unbending process and the misalignment deviation of supporting rolls, occurs in the upper part of the solidified shell, which is another reason causing halfway cracks in A36-B slab. By decreasing the P and S content to be less than 50 ppm and 150 ppm, respectively, controlling B content in the range of 10 to 15 ppm, increasing the secondary cooling specific water ratio from 0.76 to 0.85 L·kg-1, and restricting roll alignment deviation to less than plus or minus 0.3 mm, halfway cracks in the boron containing slab are almost eliminated.

关键词： boron halfway crack continuous casting hot ductility solution segregation

The Ecological Effects of Young Elm Trees Belt-Pumpkin Strip Intercropping System at the Agro-Pastoral Ecotone in Northern China

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Agricultural Sciences in China 2010年第8期9卷 1183-1193页

作者： du Xiong DOU Tie-ling FENG Li-xiao ZHANG Wei-hong ZHANG Li-feng College of Agronomy Agricultural University ofHebei Baoding 071001 P.R.China Zhangbei Agricultural Resource and Ecological Environment Key Field Research Station Ministry of Agriculture Zhangjiakou 076450 P. R. China College of Plant Protection Agricultural University of Hebei Baoding 071001 P.R. China

Young elm trees belt-pumpkin strip intercropping was studied to solve the actual problem of resource losses in the large barren area resulted from reconverting cultivated land into forest in the agro-pastoral ecotone in northern China. The final objective was to realize effective utilization of the barren land with both ecological improvement and economic development. Field experiments were conducted together with laboratory analysis. The results indicated that the soil moisture level was remarkably increased in young elm trees belt-pumpkin strip intereropping because the pumpkin vines covered the gap between pumpkin planting-furrow and elm trees belt. The water use efficiency of the intercropping system was increased by 23.7-163.3% as compared with the single cropping. Elm trees belt-pumpkin strip intercropping changed the sequential succession trend of the grasses growing in the gap of the pumpkin planting-furrow. The annual grasses become the dominant vegetation. The nutritive value as fodder and yield of the annual grasses were also increased remarkably. The biomass of pumpkin, elm trees and grasses under intercropping increased by 24.4, 28.4 and 144.4%, respectively, as compared with those under single cropping. The land use efficiency was increased by 132%. It was also indicated that the soil erosion from the intercropping land was not increased due to pumpkin plantation. The differences in the soil erosion among intercropped area, elm trees belt and pumpkin strip with single cropping were not remarkable. Therefore, it was concluded that young elm trees belt-pumpkin strip intercropping is an effective way to utilize the barren land between the young elm trees belt and realize synergistic enhancement of ecological benefit and economic profit.

关键词： ecotone young elm trees belt-pumpkin strip intercropping water use efficiency land use efficiency ecological benefit economic profit

Bi-, Y-Codoped TiO2 for Carbon Dioxide Photocatalytic Reduction to Formic Acid under Visible Light Irradiation

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Chinese Journal of Chemistry 2018年第6期36卷 538-544页

作者： Pengju du Tongming Su Xuan Luo Xiantai Zhou Zuzeng Qin Hongbing Ji Jianhua Chen School of Chemistry and Chemical Engineering Guangxi Key Laboratory of Petrochemical Resource Processing and Process Intensification Tech- nology Guangxi University Nanning Guangxi 530004 China School of Chemistry Sun Yat-sen University Guangzhou Guangdong 510275 China College of Resources Environment and Materials Guangxi University Nanning Guangxi 530004 China

Bi- and Y-codoped TiO2 photocatalysts were synthesized through a sol-gel method, and they were applied in the photocatalytic reduction of CO2 to formic acid under visible light irradiation. The results revealed that, after doping Bi and Y, the surface area of TiO2 was increased from 5.4 to 93.1 m2/g when the mole fractions of doping Bi and Y were 1.0% and 0.5%, respectively, and the lattice structures of the photocatalysts changed and the oxygen vacancies on the surface of the photocatalysts formed, which would act as the electron capture centers and slow down the recombination of pho- to-induced electron and hole. The photocurrent spectra also proved that the photocatalysts had better electronic transmission capacities. The HCOOH yield in CO2 photocatalytic reduction was 747.82 μmol/gcat by using 1% Bi-0.5% Y-TiO2 as a photocatalyst. The HCOOH yield was 1.17 times higher than that by using 1% Bi-TiO2, and 2.23 times higher than that by using pure TiO2. Furthermore, the 1% Bi-0.5% Y-TiO2 showed the highest apparent quantum efficiency （AQE） of 4.45%.

关键词： bismuth yttrium titanium dioxide photocatalytic reduction carbon dioxide formic acid

Protein Disulfide Isomerase and Its Potential Function on Endoplasmic Reticulum Quality Control in Diatom Phaeodactylum tricornutum

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Phyton-International Journal of Experimental Botany 2024年第1期93卷 137-150页

作者： Yanhuan Lin Hua du Zhitao Ye Shuqi Wang Zhen Wang Xiaojuan Liu Zhangzhou Institute of Technology ZhangzhouChina Guangdong Provincial Key Laboratory of Marine Biotechnology Guangdong Provincial Key Laboratory of Marine Disaster Prediction and PreventionCollege of SciencesShantou UniversityShantouChina

PDI is a molecular chaperone and plays an important role in Endoplasmic Reticulum quality control (ERQC).PDI participates in the refolding of the misfolded/unfolded proteins to maintain cellular homeostasis under differentstresses. However, bioinformatic characteristics and potential functions of PDIs in diatom Phaeodactylumtricornutum (Pt) are still unknown so far. Hence, the genome-wide characteristics of PtPDI proteins in P. tricornutumwere first studied via bioinformatic and transcriptomic methods. 42 PtPDI genes were identified from thegenome of P. tricornutum. The motif, protein structure, classification, number of introns, phylogenetic relationship,and the expression level of 42 PtPDI genes under the tunicamycin stress were analyzed. A pair of tandemduplicated genes (PtPDI15 and PtPDI18) was observed in P. tricornutum. The 42 PtPDIs with different genecharacteristics were divided into three independent clades, indicating different evolutional relationships and functionsof these PtPDIs. The 14 up-regulated PtPDI genes under the tunicamycin treatment might have a positiveeffect on the ER quality control of the unfolded/misfolded proteins, while the 7 down-regulated PtPDIs mightnegatively affect the ERQC. The characteristics of all 42 PtPDIs and their proposed working model here providea comprehensive understanding of the PtPDIs gene family. The differential expression of 21 PtPDIs will be usefulfor further functional study in the ERQC.

关键词： Protein disulfide isomerase gene family Endoplasmic Reticulum quality control Phaeodactylum tricornutum

Mineralogy,sulfur isotopes and infrared microthermometric study of the Leishan-Rongjiang antimony ore field,SW China

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Acta Geochimica 2017年第2期36卷 339-352页

作者： Jun Chen Ruidong Yang Junbo Gao Lulin Zheng Lijuan du Minggang Yuan Huairui Wei College of Resources and Environmental Engineering Guizhou University Guiyang 550025 People's Republic of China State Key Laboratory of Ore Deposit Geochemistry Institute of Geochemistry Chinese Academy of Science Guiyang 550002 People's Republic of China Guizhou Qinglong Antimony Mining Industry District Qinglong Country 561400 Guizhou Province People's Republic of China

The Leishan-Rongjiang antimony ore field(LAOF) is in a unique geotectonic location in the uplift between the Youjiang and Xiangzhong *** paper focuses on two representative deposits in the LAOF:the Bameng and Peize antimony(Sb) *** analyzed fluid inclusions(FIs) in stibnite and coexisting quartz,as well as the sulfur isotopic composition of stibnite,to better understand the nature of the ore-forming fluid and the metallogenic *** FIs data from samples of the stibnite and coexisting quartz indicate that the ore-forming fluids were characterized by low-temperature(150-210 ℃),low-salinity(1.5 wt%-6.0 wt%NaCl equiv.),and low-density(0.872-0.961 g/cm^3).The δ^(34)S values of stibnite(-8.21‰ to 3.76‰,average =-6.30‰)fall in between the sulfur isotopic compositions of the mantle and of biogenic sulfur in sedimentary ***,the δ^(34)S_(∑s) values(-4.41 ‰ to +0.04‰,average =-2.49‰) of the ore-forming fluids are generally closer to the sulfur isotopic composition of the mantle source,indicating that the sulfur in the LAOF was mainly sourced from the mantle,but with possible involvement of biogenic *** addition,FIs petrography and ore deposit geology show that fluid boiling resulted from an abrupt decrease in pressure,which may have triggered the precipitation of *** conclude that low-temperature,dilute hydrothermal fluids with mixed origins migrated along the regional fault and interacted with the wall rock,extracting the ore-forming ***,the oreforming fluids were injected into the fault fracture zones.

关键词： Stibnite Fluid inclusions Infrared microthermometry Sulfur isotopes Leishan-Rongjiang antimony ore field Guizhou

Structure-Activity Relationship, Cytotoxicity and Mode of Action of 2-Ester-substituted 1,5-Benzothiazepines as Potent Antifungal Agents

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Chinese Journal of Chemistry 2013年第10期31卷 1305-1314页

作者： Wang Kang Xingqiong du Lanzhi Wang Lijuan HU Yuhuan Dong Yanqing Bian Yuan Li College of Chemistry & Material Science Hebei Normal University Sho'iazhuang Hebei 050024 China College of Life Sciences Hebei Normal University Sho'iazhuang Hebei 050024 China Department of Chemistry Tangshan Normal University Tangshan Hebei 063000 China

Our studies examined the structural features responsible for the antifungal activity of 2-ethoxycarbonyl-1,5- benzothiazepine （7a）. Three series of 1,5-benzothiazepine derivatives were synthesized and screened for their antifungal activity. The results suggested that the ethoxycarbonyl group at the 2 position and the imine moiety on the seven-membered ring are essential for activity. The most potent of the synthesized analogues （7a, 7b） were further studied by evaluating their cytotoxicity and mode of action （for 7a）. The results showed that compounds 7a and 7b were relatively safe for BV2 cells, but compound 7a interfered with Cryptococcus neoformans cell wall integrity by increasing the chitinase activity. Therefore, compound 7a was considered safe as an antifungal agent for animal cells.

关键词： 1,5-benzothiazepines antifungal agents antifungal mechanism cytotoxicity structure-activity relationships

Therapeutic potential of bone marrow-derived mononuclear cells for experimental cerebral ischemia in mice

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Neural Regeneration Research 2010年第23期5卷 1765-1772页

作者： Xuemei Zhang Fang du Dan Yang Chunjiang Yu Xiangnan Huang Wei Liu Jin Fu Department of Neurology Second Affiliated Hospital Harbin Medical University Harbin 150086 Heilongjiang Province China Center of Research and Experiment Second Affiliated Hospital Harbin Medical University Harbin 150086 Heilongjiang Province China

Bone marrow mononuclear cells （BMMCs） can be directly harvested from the donor, allowing for easier application compared with bone marrow mesenchymal stem cells. The present study hypothesized that BMMC transplantation could ameliorate cerebral ischemia in a mouse model. BMMCs were double-labeled with PKH26 and 4＇, 6-diamidino-2-phenylindole （DAPI）, followed by infusion into mice via the tail vein to induce focal cerebral ischemia. At 14 days after transplantation, morphological and neurofunctional recovery were analyzed. PKH26 and DAPI double-positive BMMCs were detected in the cerebral hemisphere of all transplantation mice. Following BMMC administration, there was significant difference in neurofunctional recovery, but no significant difference in survival rates between BMMC-treated mice and other mice. These results demonstrate that transplanted BMMCs migrate to brain tissue and promote neurological function recovery in a mouse model of cerebral ischemia.

关键词： cerebral ischemia bone marrow cell homing cell therapy neuroprotection

Study of circumferential resection margin in patients with middle and lower rectal carcinoma

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World Journal of Gastroenterology 2007年第24期13卷 3380-3383页

作者： Ze-Yu Wu Jin Wan Jing-Hua Li Gang Zhao Lin Peng Yuan Yao Jia-Lin du Quan-Fang Liu Zhi-du Wang Zhi-Ming Huang Hua-Huan Lin Department of General Surgery Guangdong Provincial People's Hospital Guangzhou 510080 Guangdong Province China Zhong Shan Medical-College Sun Yat-sen University Guangzhou 510089 Guangdong Province China Department of Pathology Guangdong Provincial People's Hospital Guangzhou 510080 Guangdong Province China

AIM: To clarify the relationship between circumferential resection margin status and local and distant recurrence as well as survival of patients with middle and lower rectal carcinoma. The relationship between circumferential resection margin status and clinicopathologic characteristics of middle and lower rectal carcinoma was also evaluated. METHODS: Cancer specimens from 56 patients with middle and lower rectal carcinoma who received total mesorectal excision at the Department of General Surgery of Guangdong Provincial People's Hospital were studied. A large slice technique was used to detect mesorectal metastasis and evaluate circumferential resection margin status. RESULTS: Local recurrence occurred in 12.5% (7 of 56 cases) of patients with middle and lower rectal carcinoma. Distant recurrence occurred in 25% (14 of 56 cases) of patients with middle and lower rectal carcinoma. Twelve patients (21.4%) had positive circumferential resection margin. Local recurrence rate of patients with positive circumferential resection margin was 33.3% (4/12), whereas it was 6.8% (3/44) in those with negative circumferential resection margin (P = 0.014). Distant recurrence was observed in 50% (6/12) of patients with positive circumferential resection margin; conversely, it was 18.2% (8/44) in those with negative circumferential resection margin (P = 0.024). Kaplan-Meier survival analysis showed significant improvements in median survival (32.2 ± 4.1 mo, 95% CI: 24.1-40.4mo vs 23.0 ± 3.5 mo, 95% CI: 16.2-29.8 mo) for circumferential resection margin-negative patients over circumferential resection margin-positive patients (log-rank, P < 0.05). 37% T3 tumors examined were positive for circumferential resection margin, while only 0% T1 tumors and 8.7% T2 tumors were examined as circumferential resection margin. The difference between these three groups was statistically significant (P = 0.021). In 18 cancer specimens with tumor diameter ≥ 5 cm 7 (38.9%) were detected as positive c

关键词： Middle and lower rectal carcinoma Circumferential resection margin Prognosis