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Interleukin-2 expression and glioma cell proliferation following Vaccinia vector gene transfection in vivo

Neural Regeneration Research 2008年第4期3卷 415-417页

作者： Xiaogang Wang xuezhong Wei Jiangqiu liu Department of Neurosurgery General Hospital Shenyang Military Area Command of Chinese P~A Shenyang 110015 Liaoning ProvinceChina

BACKGROUND： The effectiveness of gene therapy is closely related to the efficiency of vector transfection and expression. OBJECTIVE： This study was designed to transfect a human brain glioma cell line with recombinant Vaccinia virus expressing the interleukin-2 （rVV-IL-2） gene, and to observe IL-2 expression and glioma cell proliferation potential after transfection. DESIGN： Experimental observation. SETTING： Department of Neurosurgery, Shenyang Military Area Command of Chinese PLA. MATERIALS： The rVV-IL-2 vectors were obtained through homologous recombination and screening in the Second Military Medical University of Chinese PLA. The human brain glioma cell line and IL-2-dependent cells were produced by the Second Military Medical University of Chinese PLA. Human IL-2 was produced by Genzyme Corporation. METHODS： At passage day l, Veto cells were amplified l ; 1 for virus and cells. A human brain glioma cell line was transfected using amplified Vaccinia viral vectors at varying multiplicities of infection （MOI）. At 2, 4, 6, 8, 12, and 24 hours post-transfection, superuatant was collected to determine by MTT assay IL-2 expression levels in IL-2 dependent cells. The transfected and non-transfected cells were divided into 4 groups, namely MOI1 ： 1, MOI 5 ： 1, MOI 10 ： 1, and control groups. MAIN OUTCOME MEASURES： IL-2 expression at different time points after transfection of human brain glioma cells with varying MOI of Vaccinia viral vectors; in vitro proliferation capacity of human brain glioma cells among the 4 groups. RESULTS： IL-2 expression was detectable 4 hours after Vaccinia viral vector transfection and reached 300 kU/L by 8 hours. There was no significant difference in the proliferating rate of human brain glioma cells among the 4 groups （P 〉 0.05）. CONCLUSION： Vaccinia viral vectors can transfect human brain glioma cells in vitro and express high levels of IL-2. Vaccinia virus and high IL-2 expression do not influence the proliferation rate of human brain g

关键词： Vaccinia virus vector glioma cell proliferation potential in vitro interleukin-2

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Cochlear implants in genetic deafness

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Journal of Otology 2014年第4期9卷 156-162页

作者： xuezhong liu Department of Otolaryngology University of Miami Miller School of Medicine

Genetic defects are one of the most important etiologies of severe to profound sensorineural hearing loss and play an important role in determining cochlear implantation *** the pathogenic mutation types of a number of deafness genes have been cloned,the pathogenesis mechanisms and their relationship to the outcomes of cochlear implantation remain a hot research *** auditory performance is considered to be affected by the etiology of hearing loss and the number of surviving spiral ganglion cells,as well as *** research advances in cochlear implantation for hereditary deafness,especially the relationship among clinic-types,genotypes and outcomes of cochlear implantation,will be discussed in this review.

关键词： Connexin Cx26/Cx30 Usher syndrome Mitochondria genes Waardenburg syndrome Auditory neuropathy

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Neuroprotective effects of combined lead and cadmium,as well as N-acetylcysteine,on cerebral cortical neurons following lipid peroxidation injury

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Neural Regeneration Research 2009年第11期4卷 907-911页

作者： Yumei Zhang Hao Lu Yan Yuan xuezhong liu Zongping liu College of Veterinary Medicine Yangzhou University Yangzhou 225009 Jiangsu Province China College of Veterinary Medicine Northwest Sci- Tech University of Agriculture and Forestry Yangling 712100 Shaanxi Province China

BACKGROUND： Studies have reported the antioxidant effects of lead and cadmium in the central nervous system, but very few have addressed the combined toxicity of lead and cadmium. The mechanisms by which these combined heavy metals are toxic, as well as how to protect cells from these agents, remains poorly understood. OBJECTIVE： Primary cultured rat cortical neurons were used to determine the effects of combined lead and cadmium on levels of glutathione peroxidase （GSH-Px）, superoxide dismutase （SOD）, catalase （CAT）, and acetylcholinesterase （ACHE）, as well as malondialdehyde （MDA）, and to evaluate the neuroprotective effects of N-acetylcysteine （NAC）. DESIGN, TIME AND SETTING An in vitro toxicological observation was performed at the Comparative Medicine Center of Yangzhou University from August 2007 to April 2008. MATERIALS： Lead acetate, cadmium acetate, and NAC were purchased from Sigma-Aldrich （St. Louis, USA）. Commercial kits of GSH-Px, SOD, CAT, ACHE, and MDA were purchased from Nanjing Jiancheng Bioengineering Institute, Nanjing, China. METHODS： The cerebral cortical neurons were isolated from newborn Sprague dawley rats at 24 hours after birth and primary cultured for 6 days. Thereafter, the cells were treated with a range of cadmium doses （0, 5.0, and 10.0μmol/L）, lead doses （0, 1.0, and 2.0 μmol/L）, or a combination of the two for 12 hours at 37℃in a 5% CO2 incubator, respectively. In addition, the cells were incubated with different doses of cadmium and/or lead and （0 and 50 μmol/L） NAC for 12 hours to assess the protective effects on cell survival. MAIN OUTCOME MEASURES： The activity of SOD, GSH-Px, CAT, and ACHE, as well as MDA content, in the cell lysates was detected using commercial kits. RESULTS： At 12 hours after treatment, compared to the control group, activity of GSH-Px, SOD, and AChE in the lead, cadmium, or combined treated cells was significantly decreased with increasing doses of cadmium/or lead （P 〈 0.05）, but CAT activity and MDA levels

关键词： lead cadmium cortical neurons N-acetylcysteine lipid peroxidation

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地方应用型大学协同育人体制机制新探

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国家教育行政学院学报 2017年第9期 67-72页

作者：刘学忠皖西学院安徽六安237012

协同育人是新时期地方应用型大学人才培养的新途径。当前,地方高校协同育人存在主体单一、合作内容窄化、动力机制弱化、协作机制软化、评价机制匮乏等问题。为寻求破解之道,地方应用型大学应加强主体先进性与适切性建设,实现协同育人... 详细信息

协同育人是新时期地方应用型大学人才培养的新途径。当前,地方高校协同育人存在主体单一、合作内容窄化、动力机制弱化、协作机制软化、评价机制匮乏等问题。为寻求破解之道,地方应用型大学应加强主体先进性与适切性建设,实现协同育人体制突破;强化四大运行机制建设,确保协同育人机制通畅;设立学科专业"特区",一校一策,试点推进。同时,政府、企业、社会各利益相关方应协同发力,共同推进协同育人新模式的发展。

关键词：地方应用型大学协同育人体制机制实践探索

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Electrophysiology and genetic testing in the precision medicine of congenital deafness:A review

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Journal of Otology 2021年第1期16卷 40-46页

作者： Kevin Y.Zhan Oliver F.Adunka Adrien Eshraghi William J.Riggs Sandra M.Prentiss Denise Yan Fred F.Telischi xuezhong liu Shuman He Department of Otolaryngology e Head&Neck Surgery The Ohio State University Wexner Medical CenterColumbusOHUSA Department of Audiology Nationwide Children’s HospitalColumbusOHUSA Department of Otolaryngology e Head&Neck Surgery University of Miami Miller School of MedicineMiamiFLUSA Dr.John T.MacDonald Foundation Department of Human GeneticsUniversity of Miami Miller School of MedicineMiamiFLUSA

Background:Congenital hearing loss is remarkably heterogeneous,with over 130 deafness genes and thousands of variants,making for innumerable genotype/phenotype *** both the pathophysiology of hearing loss and molecular site of lesion along the auditory pathway permits for significantly individualized *** techniques such as electrocochleography(ECochG)and electrically-evoked compound action potentials(eCAP)are being studied to localize pathology and estimate residual cochlear *** *** review describes the expanding roles of genetic and electrophysiologic evaluation in the precision medicine of congenital hearing *** basics of genetic mutations in hearing loss and electrophysiologic testing(ECochG and eCAP)are reviewed,and how they complement each other in the diagnostics and prognostication of hearing *** together,these measures improve the understanding of insults to the auditory system,allowing for individualized counseling for CI candidacy/outcomes or other habilitation ***:Despite tremendous discovery in deafness genes,the effects of individual genes on neural function remain poorly *** the understanding between molecular genotype and neural and functional phenotype is paramount to interpreting genetic results in clinical *** future hearing healthcare provider must consolidate an ever-increasing amount of genetic and phenotypic information in the precision medicine of hearing loss.

关键词： Electrophysiology Genetics Hearing loss ECAP ECocHG

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低温多效海水淡化实验装置的工艺开发

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水处理技术 2017年第5期43卷 114-117页

作者：刘学忠中国电子工程设计院北京市低温多效热法海水淡化工程技术研究中心北京100142

介绍了国投集团自主开发的曹妃甸港1 000 t/d低温多效蒸发海水淡化实验装置的工艺流程、工艺参数及运行效果,并对调试过程中出现的问题提出合理的解决建议。运行结果显示,各项性能指标达到设计预期目标,产品水达到国家生活饮用水卫生标... 详细信息

介绍了国投集团自主开发的曹妃甸港1 000 t/d低温多效蒸发海水淡化实验装置的工艺流程、工艺参数及运行效果,并对调试过程中出现的问题提出合理的解决建议。运行结果显示,各项性能指标达到设计预期目标,产品水达到国家生活饮用水卫生标准(GB 5749-2006)的要求。

关键词：低温多效蒸发海水淡化工艺效果

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换流阀内水冷系统泄漏电流对散热器腐蚀的影响

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高电压技术 2020年第5期46卷 1781-1790页

作者：刘学忠王晨星刘宁焦秀英西安交通大学电力设备电气绝缘国家重点实验室西安710049 西安西电电力系统有限公司西安710065

换流阀内冷却系统中散热器的腐蚀会导致均压电极结垢并引发漏水和散热失灵等事故,严重威胁高压直流输电系统的安全可靠运行。为了研究水路泄漏电流对铝散热器腐蚀的影响,搭建了由实际散热器组成的模拟阀冷平台,并模拟现场条件进行了运... 详细信息

换流阀内冷却系统中散热器的腐蚀会导致均压电极结垢并引发漏水和散热失灵等事故,严重威胁高压直流输电系统的安全可靠运行。为了研究水路泄漏电流对铝散热器腐蚀的影响,搭建了由实际散热器组成的模拟阀冷平台,并模拟现场条件进行了运行试验;然后在隔离水箱中进行了铝片试样的电解腐蚀试验,利用失重法和显微观测等手段分析了阴、阳极试样的腐蚀量和腐蚀形貌。结果表明,冷却水路泄漏电流是导致或加剧散热器腐蚀的主导因素,且引起的腐蚀量在散热器腐蚀总量所占比重随着运行时间增加而增加。泄漏电流对同一阀段中不同电位处散热器腐蚀的影响机理存在差异:阀段高电位处散热器只受到阳极泄漏电流的影响,发生具有点蚀形貌特征的杂散电流腐蚀;而低电位处散热器则在阴极泄漏电流作用下,发生具有均匀腐蚀形貌特征的碱性腐蚀。

关键词：高压直流阀冷系统泄漏电流散热器铝合金腐蚀

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三倍浓缩低温多效海水淡化装置的工艺设计及验证

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工业水处理 2017年第8期37卷 105-108页

作者：刘学忠中国电子工程设计院北京市低温多效热法海水淡化工程技术研究中心北京100142

对三倍浓缩低温多效海水淡化技术的工艺流程、参数及运行效果进行了介绍。充分考虑高浓缩倍率的结垢风险,设计采用海水顺流流程,对蒸发器效数、效间传热温差、海水喷淋密度等进行了优化,并将一套二倍浓缩1 000 t/d低温多效海水淡化装置... 详细信息

对三倍浓缩低温多效海水淡化技术的工艺流程、参数及运行效果进行了介绍。充分考虑高浓缩倍率的结垢风险,设计采用海水顺流流程,对蒸发器效数、效间传热温差、海水喷淋密度等进行了优化,并将一套二倍浓缩1 000 t/d低温多效海水淡化装置进行技术改造,对三倍浓缩工艺进行了试验验证,测试了蒸发器的抗结垢性能和耐腐蚀性能。运行结果显示,浓缩比、造水比等各项性能指标达到预期目标。

关键词：低温多效蒸发海水淡化海水浓缩

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