检索结果-南通市图书馆

Bio-inspired peptide-Au cluster applied for mercury(II) ions detection

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science China Chemistry 2015年第5期58卷 819-824页

作者： Yaling Wang Yanyan Cui Ru Liu Fuping gao Liang gao xueyun gao Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety Institute of High Energy Physics Chinese Academy of Sciences College of Materials Science and Opto-electronic Technology University of Chinese Academy of Sciences

Mercury ion （Hg2＋） pollution exists in water, soil, and food. By interacting with the thiol groups in protein, Hg2＋ ions can ac- cumulate in ways that cause serious damage to the central nervous system and threaten human health and natural environment. Undoubtedly, Hg2＋ ion detection is a significant issue in environment and health monitoring. A variety of sensor platforms for Hg2＋ ion detection based on organic molecules, DNA, oligonucleotides, inorganic materials, etc, have been reported. In this paper, an artificial peptide PHg, with a cluster bio-mineralize sequence （CCY） and a multi-charge hydrophilic sequence is de- signed as a template for the one-step synthesis of a peptide-Au cluster probe. Briefly： the peptide PHg in situ anchors Au ions to form a peptide-Au （I） intermediate and the reaction pH with NaOH is adjusted; alter 12 h incubation at room temperature, the peptide PGg-Au nanocluster probe with red fluorescence is obtained. The probe has a super-small core size of approximately 1.26 nm and a maximum emission peak at 650 rim. The presence of Hg2＋ ions cause the fluorescence of the probe to greatly decrease. Based on the differences in fluorescence intensity of the PHg-Au nanocluster in the absence and presence of Hg2＋ ions, Hg2＋ ions could be quantitatively detected in concentrations ranging from 5 nmol/L to 1 lamol/L. The limit of detection （LOD） is 7.5 nmol/L. Compared with some interference ions such as, K＋, Mg2＋, Ca2＋, Pb2＋, Ni2＋, Fe3＋, and Cue＋, the selectivity was excellent. The sensing of Hg2＋ ion is not affected by the chelate agents： EDTA, which imparts a significant advantage in a range of applications. As a result, a simple, sensitive and oped for the detection of Hg2＋ ions. selective fluorescent assay based on peptide PHg-Au cluster is devel-

关键词： peptide-Au cluster mercury (Ⅱ) ions fluorescence detection

Assessment of the MT1-MMP expression level of different cell lines by the naked eye

在线全文

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2018年第4期61卷 492-500页

作者： Xiangchun Zhang Qing Yuan xueyun gao CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety Institute of High Energy Physics Chinese Academy of Sciences Beijing 100049 China Department of Chemistry and Chemical Engineering Beijing University of Technology Beijing 100124 China University of Chinese Academy of Sciences Beijing 100049 China

Membrane type 1 matrix metalloproteinase(MT1-MMP) is expressed in most tumors and is believed to play a key role in the development, invasion and metastasis of tumors. There is an urgent need to develop a simple method to detect the MT1-MMP expression level on cells. In this current study, we demonstrated a red emission Au cluster probes with the specific targeting of MT1-MMP on human dopaminergic neuroblastoma(SH-SY5 Y) cells. More importantly, utilizing the intrinsic enzyme-like activity of the Au cluster probes, the expression level of MT1-MMP on the SH-SY5 Y cells could be assessed by the naked eye without cell lysis and protein extraction process. Furthermore, SH-SY5 Y, human breast cancer(MCF-7), and human bronchial epithelial(16-HBE) cell lines with different MT1-MMP expression level could be distinguished using the Au cluster probes by the naked eye. Meanwhile, fluorescence intensity and Au count determined by inductively coupled plasma mass spectrometry(ICP-MS) were used to verify the feasibility of this simple analytical method. Our proposed method is rapid, convenient, and accurate and could be assessed by the naked eye. This visual assessment method of tumor-associated proteins has immense implications in sorting tumor cells.

关键词： Au clusters MT1-MMP catalyze tumor cells

Noble-metal nanocluster as enzyme-mimetic catalyst for diagnostic analysis

在线全文

学校读者我要写书评

暂无评论

Science China(Technological Sciences) 2019年第12期62卷 2306-2309页

作者： REN XiaoJun WANG ShiZheng SU DongDong gao Liang YUAN Qing gao xueyun Department of Chemistry and Chemical Engineering Beijing University of Technology

Noble-metal nanoclusters,which consist of only several to a few hundred metal atoms,have attracted a great deal of scientific interest due to their fascinating optical properties,biocompatibility and low toxicity[1].Intriguingly,na-noclusters have been recently reported possessing intrinsic enzyme-mimetic properties,mainly including peroxidase-like activity,which can convert hydrogen peroxide into hy-droxyl radicals,and catalase-like activity,which can convert hydrogen peroxide into water and *** low-cost al-ternatives to natural enzymes,nanoclusters can overcome several drawbacks of natural enzymes,such as sensitivity of catalytic activity to environmental conditions,low stability leaded by denaturation and digestion and high cost.

关键词： cluster convert catalyst

Targeted peptide-Au cluster binds to epidermal growth factor receptor (EGFR) in both active and inactive states： a clue for cancer inhibition through dual pathways

在线全文

学校读者我要写书评

暂无评论

Science Bulletin 2018年第6期63卷 349-355页

作者： Peng Zhang Jiao Zhai xueyun gao Hongkang Zhao Wenyong Su Lina Zhao School of Physics Beijing Institute of Technology Beijing 100081 China Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety Institute of High Energy Physics Chinese Academy of Sciences Beijing 100049 China Department of Chemistry and Chemical Engineering. Beijing University of Technology Beijing 100124 China

The epidermal growth factor receptor （EGFR） has become an important target protein in anticancer drug development. Meanwhile, peptide-Au cluster has been proposed as potential targeted nano-drug assembled by targeting peptide. Here, we designed and synthesized a novel peptide-Au cluster as AuloPeptides to target to EGFR. We found AumPeptides could target to the natural binding sites of all EGFRs at mem- brane in both active and inactive states by molecular simulations. Its targeted ability was further verified by the co-localization and blocking experiments. We also study the configuration modifications of both active and inactive EGFRs after binding by AumPeptides. For active EGFR, the absorbed AuloPeptide5 might replace the natural ligand in EGFR endocytosis process. Then, the peptide-Au cluster in endochylema could inhibit the cancer relating enzyme activity including thioredoxin reductasel （TrxR1） and induce the oxidative stress mediated apoptosis in tumor cells. For inactive EGFR, it was retained in inactive state by Au10 Peptides binding to inhibit dimerization of EGFR for anticancer. Both pathways might be applied in anticancer drug development based on the theoretical and experimental study here.

关键词： peptide-Au cluster EGFR Targeted binding Anticancer

CLDN18.2-targeted molecular imaging and precision therapy of gastrointestinal tumors

在线全文

学校读者我要写书评

暂无评论

Journal of Pharmaceutical Analysis 2023年第9期13卷 955-957页

作者： Yan Chen Zhi Yang xueyun gao Hua Zhu The Ministry of Education Key Laboratory of Carcinogenesis and Translational Research NMPA Key Laboratory for Research and Evaluation of RadiopharmaceuticalsDepartment of Nuclear MedicinePeking University Cancer Hospital&InstituteBeijing100142China Faculty of Environment and Life Beijing University of TechnologyBeijing100124China

Claudin-18.2(CLDN18.2)is a tight junction protein that is typically expressed only in normal gastric mucosal *** malignancy occurs,cell polarity is lost,intercellular adhesion structures are destroyed,and CLDN18.2 is therefore exposed to the surface of tumor cells[1].CLDN18.2 is specifically expressed in approximately 40%of human epidermal growth factor receptor 2(HER2)-negative gastrointestinal cancers,making it a promising emerging therapeutic *** recently reported,zolbetuximab,an CLDN18.2 targeting antibody,has shown amazing efficacy in patients with CLDN18.2-positive and HER2-negative gastric cancers[2].In clinical practice,the assessment of CLDN18.2 expression status is crucial to the determination of treatment regimen for *** imaging can be used as a non-invasive test for diagnosis,staging,and efficacy *** results of molecular imaging of CLDN18.2 have been published continuously,and the development of CLDN18.2 molecular probe is of great significance for the development of tumor targeted therapy(Fig.1).

关键词： CLDN1 gastrointestinal targeted

^(64)Cu radiolabeled nanomaterials for positron emission tomography(PET)imaging

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2022年第7期33卷 3349-3360页

作者： Xueqian Chen Wenchao Niu Zhongying Du Yong Zhang Dongdong Su xueyun gao Department of Chemistry and Biology Faculty of Environment and LifeBeijing University of TechnologyBeijing 100124China

The prevalence of positron emission tomography(PET)imaging has advanced biomedical applications for its ultrahigh sensitivity,deep tissue penetration and quantitative visualization of diseases in vivo.^(64)Cu with ideal half-life and decay characteristics has been designed as radioactive probes for disease *** currently reported ^(64)Cu-labeled nanomaterials have the advantages of long circulation time in serum,good biocompatibility and mature preparation methods,and have been used in vivo PET imaging,biodistribution and pharmacokinetic monitoring,and imaging guided *** the same time,suitable carrier characteristics and radiolabeling strategies are particularly important in the ^(64)Cu PET imaging *** this review,we summarize different imaging probe designs and ^(64)Cu radiolabeling strategies,as well as their eventual applications in *** potential challenges and prospects of ^(64)Cu labeled nanomaterials are also described,which provides broad prospects for radiolabeling strategies and further applications.

关键词： ^(64)Cu Positron emission tomography Chelating agent-free Radiolabeling strategies Imaging guided therapy

Effects of RE(La,Ce)on fcc-bcc martensitic transformation of iron via Bain transformation path

在线全文

学校读者我要写书评

暂无评论

Journal of Rare Earths 2022年第11期40卷 1812-1818,I0006页

作者： Haiyan Wang xueyun gao Lei Xing Tingting Zhai Meng Lv Huiping Ren Huihui Wei Jianguo Zhi School of Materials and Metallurgy Inner Mongolia University of Science and TechnologyBaotou 014010China Key Laboratory of Integrated Exploitation of Bayan Obo Multi-Metal Resource Inner Mongolia University of Science and TechnologyBaotou 014010China Inner Mongolia Baotou Steel Union Co. Ltd. Inner Mongolia Enterprise Key Laboratory of Rare Earth Steel Products Research and Development Baotou 014010China

The fundamental understanding of the effects of rare earth(RE)on the phase transformation of Fe-based alloys is essential in the development of advanced RE-containing high strength *** this work,we investigated the effects of La and Ce on the martensitic phase transformation of Fe in terms of the driving force and minimum energy path(MEP)using the first-principles *** calculations of formation energy show that the RE substitution increases the stabilization of fcc-Fe and decreases that of bcc-Fe,thus decreasing the driving force of phase ***,the analysis based on the generalized solid-state nudged elastic band(G-SSNEB)method indicates that in the RE-containing systems,an additional energy barrier appears when the phase transformation proceeds along the Bain transition path to c/a ratio of 1.14 in the FM state,which is associated with a sudden decrease in magnetization due to the hybridization between the orbits of the RE atoms and those of the nearest neighbor Fe *** results of potential-energy surfaces(PES)confirm that magnetic ordering and lattice volume change simultaneously with the Bain transition,and in comparison to pure Fe,the variation between the onset and the end structural volumes of the phase transformation is slight for the REcontaining Fe systems.

关键词： First-principles calculations Rare earth Fe Generalized solid-state nudged elastic band Martensitic transformation

Specific detection and effective inhibition of a single bacterial species in situ using peptide mineralized Au cluster probes

在线全文

学校读者我要写书评

暂无评论

Science China Chemistry 2018年第5期61卷 627-634页

作者： Xiangchun Zhang Li Liu Ru Liu Jing Wang Xuhu Hu Qing Yuan Juanjuan Guo Gengmei Xing Yuliang Zhao xueyun gao CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety Institute of High Energy Physics Chinese Academy of Sciences Beijing 100049 China Department of Chemistry and Chemical Engineering Beijing University of Technology Beijing 100124 China University of Chinese Academy of Sciences Beijing 100049 China

Increasingly serious microbial infections call for the development of new simpler methods for the precise diagnosis and specific inhibition of such pathogens. In this work, a peptide mineralized Au cluster probe was applied as a new simplified strategy to both recognize and inhibit a single bacteria species of Staphylococcus aureus(S. aureus) simultaneously. The probes are composed of peptides and Au clusters. Moreover, the peptides specifically target S. aureus cells and the Au clusters provide fluorescent imaging and have an antibacterial effect. These new probes enable the simultaneous specific detection and effective destruction S. aureus cells in situ.

关键词： Au cluster probes single bacteria species S aureus specific detection effective inhibition

Broken electron transfer pathway in enzyme:Gold clusters inhibiting TrxR1/Trx via cell studies and theory simulations

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2022年第7期33卷 3488-3491页

作者： Wenchao Niu Zhongying Du Chunyu Zhang Deting Xu Jiaojiao Li Minghui Sun Liyuan Wu Haodong Yao Lina Zhao xueyun gao Department of Chemistry and Biology Beijing University of TechnologyBeijing 100124China CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety Institute of High Energy PhysicsChinese Academy of SciencesBeijing 100049China University of Chinese Academy of Sciences Beijing 100049China

Thioredoxin reductase 1(TrxR1)is over activity in tumor cell to maintain their redox *** gold clusters have great potential in antitumor drug as they could well inhibit TrxR1,the molecular mechanism has not been disclosed *** this work,we revealed gold clusters can well inhibit the activity of TrxR1 in lung tumor cells and further disclosed the inhibition mechanism by using computational simulation *** firstly inferred the binding sites of gold in the hydrophobic cavities on *** simulation results show that the gold ion(released from Au cluster)interact with–SH of Cys189 in TrxR1,this greatly increase the distance between the C-terminal redox center of TrxR1 and the Trx redox center,thereby destroy the electron transfer pathway between *** electron transfer destroying mechanism is different from the previous hypothesis that gold binds to the Sec498 of TrxR1 which has never been proved by experimental and theory *** work provides a new understanding of the gold clusters to inhibit TrxR1 activity.

关键词： Enzyme inhibition Electron transfer Thioredoxin reductase 1 Gold clusters Molecular dynamics Density functional theory