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不同循环次数凝胶用水的理化性质及其对油炸鲢鱼丸表面结构的影响

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中国渔业质量与标准 2022年第5期12卷 1-10页

作者：李晴李雪宇刘月月马长伟洪惠中国农业大学食品科学与营养工程学院北京100083 中国农业大学兴化健康食品产业研究院江苏兴化225700

循环凝胶用水生产的鲢鱼丸经油炸后表面经常会产生孔洞和皱缩,影响鱼丸美观,降低鱼丸品质。为优化生产工艺、提高循环凝胶用水生产的油炸鱼丸品质,本研究基于工厂油炸鱼丸生产参数,模拟油炸鲢鱼鱼丸的生产过程,探究循环凝胶用水对鱼丸... 详细信息

循环凝胶用水生产的鲢鱼丸经油炸后表面经常会产生孔洞和皱缩,影响鱼丸美观,降低鱼丸品质。为优化生产工艺、提高循环凝胶用水生产的油炸鱼丸品质,本研究基于工厂油炸鱼丸生产参数,模拟油炸鲢鱼鱼丸的生产过程,探究循环凝胶用水对鱼丸表面性状的影响。本研究对比了实验室模拟和工厂制作油炸鱼丸的循环凝胶用水pH、蛋白质含量和等电点指标,结合SDS-PAGE和Nano-HPLC-MS/MS技术对凝胶用水中的蛋白质组成进行鉴定。此外,通过向纯净水中添加蛋清和降低pH来验证不同蛋白含量和pH的凝胶用水对鱼丸表面性状的影响。结果表明,随着生产批次的增加,实验室模拟和工厂连续制作油炸鱼丸凝胶用水的pH都不断下降,蛋白质含量逐渐增加,等电点均维持在4.55~4.65,凝胶用水循环利用次数越多,生产出来的油炸鱼丸表面孔洞越多,皱缩越严重,且凝胶用水中沉积的蛋白质主要是卵白蛋白。增加凝胶用水中的蛋清含量、降低凝胶用水pH均会导致油炸鱼丸表面性状劣化,蛋清的添加会造成鱼丸更严重的表面性状劣化。油炸鱼丸表面性状劣化的主要原因是循环凝胶用水中蛋白质含量的增加和pH的降低,蛋白质主要是来自于鱼丸制作配料蛋清中的卵白蛋白。

关键词：油炸鱼丸循环凝胶用水表面性状质谱鉴定卵白蛋白

军队医院护士卫勤保障胜任力评价指标体系构建

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护理管理杂志 2021年第11期21卷 810-814页

作者：蒋金李雪玉赵湘宁中国人民解放军北部战区总医院神经外科沈阳市111000 中国人民解放军北部战区总医院护理部沈阳市111000

目的构建军队医院护士卫勤保障胜任力评价指标体系。方法在文献回顾的基础上,运用Delphi专家咨询法对20名护理专家进行两轮函询,拟订军队医院护士卫勤保障胜任力评价指标体系,并统计分析专家函询结果。结果两轮专家咨询问卷的有效回收... 详细信息

目的构建军队医院护士卫勤保障胜任力评价指标体系。方法在文献回顾的基础上,运用Delphi专家咨询法对20名护理专家进行两轮函询,拟订军队医院护士卫勤保障胜任力评价指标体系,并统计分析专家函询结果。结果两轮专家咨询问卷的有效回收率分别95.24%、100.00%;专家权威系数分别为0.849,0.911;两轮咨询的专家意见协调系数分别为0.447、0.464(P<0.01),各指标的变异系数均<20%。最终形成军队医院护士卫勤保障胜任力评价指标体系,包括5个一级指标、18个二级指标、50个三级指标。结论胜任力评价指标体系具有科学性和可靠性,可为军队医院管理部门精准培训战备护士队伍、精准评价护士卫勤保障力水平、精准抽组执行重大任务护理人员提供标准和依据。

关键词：卫勤保障胜任力评价体系军队医院护士

Photo‑Energized MoS_(2)/CNT Cathode for High‑Performance li–CO_(2)Batteries in a Wide‑Temperature Range

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Nano-Micro Letters 2025年第1期17卷 160-175页

作者： Tingsong Hu Wenyi lian Kang Hu Qiuju li Xueliang Cui Tengyu Yao Laifa Shen Jiangsu Key Laboratory of Materials and Technologies for Energy Storage College of Materials Science and TechnologyNanjing University of Aeronautics and AstronauticsNanjing 210016People’s Republic of China

li–CO_(2) batteries are considered promising energy storage systems in extreme environments such as Mars;however,severe performance degradation will occur at a subzero temperature owning to the sluggish reaction ***,a photo-energized strategy adopting sustainable solar energy in wide working temperature range li–CO_(2) battery was achieved with a binder-free MoS_(2)/carbon nanotube(CNT)photo-electrode as *** unique layered structure and excellent photoelectric properties of MoS_(2) facilitate the abundant generation and rapid transfer of photo-excited carriers,which accelerate the CO_(2) reduction and li_(2)CO_(3) decomposition upon *** illuminated battery at room temperature exhibited high discharge voltage of 2.95 V and mitigated charge voltage of 3.27 V,attaining superior energy efficiency of 90.2%and excellent cycling stability of over 120 *** at an extremely low temperature of−30℃,the battery with same electrolyte can still deliver a small polarization of 0.45 V by the photoelectric and photothermal synergistic mechanism of MoS_(2)/CNT *** work demonstrates the promising potential of the photo-energized wide working temperature range li–CO_(2) battery in addressing the obstacle of charge overpotential and energy efficiency.

关键词： li-CO_(2)batteries Photo-energized Wide operation-temperature Kinetics MoS_(2)

补肾健脑针法联合草酸艾司西酞普兰治疗重性抑郁障碍疗效观察

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辽宁中医药大学学报 2022年第1期24卷 93-96页

作者：宫莉莉李冬冬李雪玉北部战区总医院辽宁沈阳110016

目的探讨补肾健脑针法联合草酸艾司西酞普兰治疗重性抑郁障碍(MDD)的效果及可能机制。方法选取2017年1月—2018年12月北部战区总医院精神心理科收治的MDD患者90例,随机分为观察组和对照组,各45例。两组均采用草酸艾司西酞普兰进行治疗,... 详细信息

目的探讨补肾健脑针法联合草酸艾司西酞普兰治疗重性抑郁障碍(MDD)的效果及可能机制。方法选取2017年1月—2018年12月北部战区总医院精神心理科收治的MDD患者90例,随机分为观察组和对照组,各45例。两组均采用草酸艾司西酞普兰进行治疗,观察组在药物基础上联合补肾健脑针法进行治疗。补肾健脑针灸法:主穴取百会、神庭、大钟,配以风池、内关、神门、足三里和三阴交。两组患者均治疗8周。治疗前后用汉密尔顿抑郁量表(HAMD-24)评估抑郁的严重程度;酶联免疫吸附试验检测血浆脑源性神经营养因子(BDNF)、神经生长因子(NGF)、5-羟色胺(5-HT)、去甲肾上腺素(NE)和5-羟吲哚乙酸(5-HIAA)表达水平;健康调查简表(SF-36)评估患者的生活质量。比较两组治疗效果和不良反应。结果治疗8周后,观察组HAMD-24评分低于对照组(P0.05)。结论补肾健脑针法可以提高草酸艾司西酞普兰治疗MDD的疗效,并且提高患者的生活质量,改善血浆BDNF、NGF、5-HT、NE和5-HIAA水平。

关键词：补肾健脑针灸草酸艾司西酞普兰重性抑郁障碍生活质量

Constraints on holographic QCD phase transitions from PTA observations

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Science China(Physics,Mechanics & Astronomy) 2025年第1期Mechanics & Astronomy) .卷 71-78页

作者： Song He li li Sai Wang Shao-Jiang Wang Center for Theoretical Physics and College of Physics Jilin University School of Physical Science and Technology Ningbo University Max Planck Institute for Gravitational Physics (Albert Einstein Institute) CAS Key Laboratory of Theoretical Physics Institute of Theoretical Physics Chinese Academy of Sciences School of Fundamental Physics and Mathematical Sciences Hangzhou Institute for Advanced StudyUniversity of Chinese Academy of Sciences (UCAS) School of Physical Sciences University of Chinese Academy of Sciences (UCAS) Theoretical Physics Division Institute of High Energy Physics Chinese Academy of Sciences

The underlying physics of QCD phase transition in the early Universe remains largely unknown due to its strong-coupling nature during the quark-gluon plasma/hadron gas transition, yet a holographic model has been proposed to quantitatively fit the lattice QCD data while with its duration of the first-order phase transition(FoPT) left undetermined. At specific baryon chemical potential, the first-order QCD phase transition agrees with the observational constraint of baryon asymmetry. It, therefore, provides a scenario for phase transition gravitational waves(GWs) within the standard model of particle physics. If these background GWs could contribute dominantly to the recently claimed common-spectrum red noise from pulsar timing array(PTA) observations, the duration of this FoPT can be well constrained, and the associated primordial black holes are still allowed by current observations.

关键词： pulsar timing array QCD phase transition gravitational waves primordial black holes

4种甘草属植物EST-SSR引物开发及其亲缘关系分析

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西北植物学报 2015年第3期35卷 480-485页

作者：李晓岚陆嘉惠谢良碧张爱霞陈晓翠李学禹石河子大学生命科学学院新疆石河子832003 新疆生产建设兵团绿洲生态农业重点实验室新疆石河子832003 石河子大学甘草研究所新疆石河子832003

通过乌拉尔甘草表达序列标签(EST)数据库查找甘草属的SSR位点,并利用Primer 3.0软件在线设计EST-SSR引物,对来自甘草属4个种22份材料的EST-SSR指纹图谱特征和聚类结果进行分析,为探讨甘草属种间亲缘关系和疑难种的分类地位提供分子依据... 详细信息

通过乌拉尔甘草表达序列标签(EST)数据库查找甘草属的SSR位点,并利用Primer 3.0软件在线设计EST-SSR引物,对来自甘草属4个种22份材料的EST-SSR指纹图谱特征和聚类结果进行分析,为探讨甘草属种间亲缘关系和疑难种的分类地位提供分子依据。结果显示:(1)去掉冗余序列后共得到441条EST序列,获得504个SSR位点,其中二核苷酸为重复单元的序列最多为350个,占69.44%,重复类型中以TC/AT、TA/AG形式的微卫星最为丰富。(2)设计的40对EST-SSR引物,均能扩增出清晰条带,其中15对引物具有多态性,在22份甘草属植物材料中共获得等位基因59个,平均每对引物检测到3.93个等位基因位点,扩增产物多态性比率为89.44%,能很好地表征甘草属种间的等位基因差异。(3)引物Primer 64对4种甘草属植物均能扩增出特异性条带,黄甘草在180和220bp 2个位点分别与光果甘草、胀果甘草基因共享,具有杂交种特征。(4)聚类分析表明,当相似性系数为0.82时,22份材料被划分为四组(与经典分类结果一致),第一组为内蒙古杭锦旗分布的乌拉尔甘草;第二组为新疆巴楚分布的光果甘草、黄甘草;第三组为新疆石河子分布的胀果甘草、黄甘草;第四组为新疆石河子分布的乌拉尔甘草;不同居群的黄甘草遗传分化较大,可能与同域分布亲本种的差异及种间的渐渗杂交有关。研究表明,开发的15对EST-SSR引物在甘草属内具有很好的适用性,可以为该属的种间亲缘关系和种内遗传分化研究及物种鉴定提供分子依据。

关键词：甘草属 EST SSR 亲缘关系

Correlation between the rock mass properties and maximum horizontal stress:A case study of overcoring stress measurements

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International Journal of Minerals,Metallurgy and Materials 2025年第1期32卷 39-48页

作者： Peng li Meifeng Cai Shengjun Miao Yuan li Yu Wang Key Laboratory of Ministry of Education for Efficient Mining and Safety of Metal Mines University of Science and Technology BeijingBeijing 100083China State Key Laboratory of Coal Resources and Safe Mining China University of Mining and TechnologyXuzhou 221116China

Understanding the mechanical properties of the lithologies is crucial to accurately determine the horizontal stress *** investigate the correlation between the rock mass properties and maximum horizontal stress,the three-dimensional(3D)stress tensors at 89 measuring points determined using an improved overcoring technique in nine mines in China were adopted,a newly defined characteristic parameter C_(ERP)was proposed as an indicator for evaluating the structural properties of rock masses,and a fuzzy relation matrix was established using the information distribution *** results indicate that both the vertical stress and horizontal stress exhibit a good linear growth relationship with *** is no remarkable correlation between the elastic modulus,Poisson's ratio and depth,and the distribution of data points is scattered and ***,there is no obvious relationship between the rock quality designation(RQD)and *** maximum horizontal stress σ_(H) is a function of rock properties,showing a certain linear relationship with the C_(ERP)at the same *** addition,the overall change trend of σ_(H) determined by the established fuzzy identification method is to increase with the increase of C_(ERP).The fuzzy identification method also demonstrates a relatively detailed local relationship betweenσ_H and C_(ERP),and the predicted curve rises in a fluctuating way,which is in accord well with the measured stress data.

关键词： overcoring stress measurements elastic modulus Poisson's ratio rock quality designation maximum horizontal stress fuzzy identification

脂肪酸结合蛋白5结合Vimentin蛋白在肝癌中的作用机制

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实用医学杂志 2024年第6期40卷 756-761,767页

作者：唐艳萍李科志蔡政民陶昊唐嘉营李学宇李炎娟曹骥广西医科大学附属肿瘤医院实验研究部南宁530021 广西区域性高发肿瘤早期防治研究重点实验室南宁530021

目的筛选和验证与脂肪酸结合蛋白5(FABP5)结合的互作蛋白,同时研究FABP5与候选蛋白的调控关系,进一步探讨FABP5在肝癌中的作用机制。方法采用免疫沉淀联合串联质谱分析方法(IP-MS)筛选出与FABP5相互结合的蛋白;通过免疫共沉淀实验(Co-... 详细信息

目的筛选和验证与脂肪酸结合蛋白5(FABP5)结合的互作蛋白,同时研究FABP5与候选蛋白的调控关系,进一步探讨FABP5在肝癌中的作用机制。方法采用免疫沉淀联合串联质谱分析方法(IP-MS)筛选出与FABP5相互结合的蛋白;通过免疫共沉淀实验(Co-IP)从外源性和内源性层面验证FABP5与候选互作蛋白的结合关系;利用RT-qPCR、Western blot和免疫荧光法观察敲低FABP5对肝癌细胞中波形蛋白(Vimentin)转录和翻译水平的影响;通过鬼笔环肽染色实验观察过表达FABP5对肝癌细胞骨架的影响。结果 IP-MS鉴定出336个与FABP5相互结合的潜在靶蛋白,结合文献,挑选出5个与肿瘤相关的候选蛋白,分别为PRDX1、PRSS3、PKM、HSP90AA1和Vimentin蛋白。通过外源性和内源性Co-IP实验证实FABP5与Vimentin蛋白存在结合关系。敲低FABP5对肝癌细胞中Vimentin mRNA的表达没有显著作用,但可抑制Vimentin蛋白的表达,而过表达FABP5会影响肝癌细胞的骨架。结论 FABP5促进肝癌细胞的迁移和侵袭,其机制可能与FABP5结合调控Vimentin蛋白和影响细胞骨架重塑有关,有望成为抗肝癌的潜在靶点,为肝癌的治疗提供新的思路。

关键词：脂肪酸结合蛋白5 波纹蛋白肝癌质谱互作蛋白

Reversible li2C2O4 product regulation in li–CO2 batteries using single-atom alloy engineering

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Science China Chemistry 2025年

作者： Xinxin Zhang Yu Wang Yafei li Jiangsu Key Laboratory of New Power Batteries Jiangsu Collaborative Innovation Centre of Biomedical Functional Materials School of Chemistry and Materials Science Nanjing Normal University

The efficiency of lithium-carbon dioxide （li–CO2） batteries is limited by the sluggish generation/decomposition kinetics of the discharge products （i.e.,li2CO3and carbon） despite the use of state-of-the-art noble metal （NM） ***2C2O4has emerged as a promising alternative product;however,the thermodynamic instability of li2C2O4causes it to generally appear as an intermediate in li2CO3pathways rather than a product during ***,using comprehensive first-principles calculations,we report tailoring the discharge products of li–CO2batteries to li2C2O4via single-atom alloy （SAA） engineering,in which NM-doped copper SAAs （NM1Cu） are constructed for *** NM1Cu catalysts display a free-atom-like narrow d-band state and unique charge distribution over the solute NM1atom and the surrounding matrix Cu *** an optimized electronic structure is substantial in Ru1Cu and Ir1Cu,which is conducive to the co-activation and coupling of *** lithiation of*CO2intermediate that breaks the charge symmetry of the central C species can further reduce the kinetic barriers of the C–C *** advantages promote li2C2O4generation on Ru1Cu and Ir1Cu while suppress its subsequent ***,they exhibit low theoretical overpotentials and enable facile delithiation under normal charging *** work proposes a universal principle of manipulating electronic structures to achieve li2C2O4product,which provides practical guidance for the development of li–CO2batteries.

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