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Silk-based hydrogel incorporated with metal-organic framework nanozymes for enhanced osteochondral regeneration

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Bioactive Materials 2023年第2期20卷 221-242页

作者： Zhicheng Cao Hongmei Wang Jialin Chen Yanan Zhang Qingyun Mo Po Zhang Mingyue Wang Haoyang liu xueyang Bao Yuzhi Sun Wei Zhang Qingqiang Yao Department of Orthopaedic Surgery Institute of Digital MedicineNanjing First HospitalNanjing Medical University210006NanjingChina School of Medicine Southeast University210009NanjingChina Department of Pharmaceutical Sciences Binzhou Medical University264003YantaiShandongChina Jiangsu Key Laboratory for Biomaterials and Devices Southeast University210096NanjingChina China Orthopedic Regenerative Medicine Group(CORMed) China

Osteochondral defects(OCD)cannot be efficiently repaired due to the unique physical architecture and the pathological microenvironment including enhanced oxidative stress and *** strategies,such as the control of implant microstructure or the introduction of growth factors,have limited functions failing to manage these complex *** we developed a multifunctional silk-based hydrogel incorporated with metal-organic framework nanozymes(CuTA@SF)to provide a suitable microenvironment for enhanced OCD *** incorporation of CuTA nanozymes endowed the SF hydrogel with a uniform microstructure and elevated *** vitro cultivation of mesenchymal stem cells(MSCs)and chondrocytes showed that CuTA@SF hydrogel accelerated cell proliferation and enhanced cell viability,as well as had antioxidant and antibacterial *** the inflammatory environment with the stimulation of IL-1β,CuTA@SF hydrogel still possessed the potential to promote MSC osteogenesis and deposition of cartilage-specific extracellular matrix(ECM).The proteomics analysis further confirmed that CuTA@SF hydrogel promoted cell proliferation and ECM *** the full-thickness OCD model of rabbit,CuTA@SF hydrogel displayed successfully in situ OCD regeneration,as evidenced by micro-CT,histology(HE,S/O,and toluidine blue staining)and immunohistochemistry(Col I and aggrecan immunostaining).Therefore,CuTA@SF hydrogel is a promising biomaterial targeted at the regeneration of OCD.

关键词： Osteochondral regeneration Metal-organic framework Nanozyme Inflammation ROS Silk

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晚期心力衰竭患者安宁疗护的研究进展

临床医学研究与实践 2024年第21期9卷 181-185页

作者：陈文博张奇钰牛少琼李雪阳刘瑞卿李转珍河南科技大学护理学院河南洛阳471003 吉林大学第一医院吉林长春130012 河南科技大学第一附属医院河南洛阳471003

心力衰竭是威胁全球公众健康的主要疾病之一,且随着人口老龄化加剧,该病的患病人数逐年增加,约有5%~10%的心力衰竭患者将进入晚期心力衰竭阶段,出现一系列顽固性症状导致生活质量大大降低。安宁疗护对缓解晚期心力衰竭患者的身心症状、... 详细信息

心力衰竭是威胁全球公众健康的主要疾病之一,且随着人口老龄化加剧,该病的患病人数逐年增加,约有5%~10%的心力衰竭患者将进入晚期心力衰竭阶段,出现一系列顽固性症状导致生活质量大大降低。安宁疗护对缓解晚期心力衰竭患者的身心症状、提高生存质量有着重要意义,然而实际接受安宁疗护的患者较少。本文综述晚期心力衰竭患者安宁疗护的需求、实施模式、影响因素及促进措施,以期为我国开展晚期心力衰竭患者安宁疗护提供参考。

关键词：晚期心力衰竭安宁疗护生存质量

Genomic Shift in Population Dynamics of mcr-1-positive Escherichia coli in Human Carriage

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Genomics, Proteomics & Bioinformatics 2022年第6期20卷 1168-1179页

作者： Yingbo Shen Rong Zhang Dongyan Shao Lu Yang Jiayue Lu Congcong liu xueyang Wang Junyao Jiang Boxuan Wang Congming Wu Julian Parkhill Yang Wang Timothy R.Walsh George F.Gao Zhangqi Shen College of Veterinary Medicine China Agricultural UniversityBeijing 100193China Guangdong Laboratory for Lingnan Modern Agriculture Guangzhou 510641China CAS Key Laboratory of Pathogenic Microbiology and Immunology Institute of MicrobiologyChinese Academy of SciencesBeijing 100101China The Second Affiliated Hospital of Zhejiang University School of Medicine Zhejiang UniversityHangzhou 310009China Department of Veterinary Medicine University of CambridgeCambridge CB30ESUK Department of Biology University of OxfordOxford OX13SZUK Chinese Center for Disease Control and Prevention Beijing 102206China

Emergence of the colistin resistance gene,mcr-1,has attracted worldwide *** the prevalence of mcr-1-positive Escherichia coli(MCRPEC)strains in human carriage showing a significant decrease between 2016 and 2019,genetic differences in MCRPEC strains remain largely *** therefore conducted a comparative genomic study on MCRPEC strains from fecal samples of healthy human subjects in 2016 and *** identified three major differences in MCRPEC strains between these two time ***,the insertion sequence ISApl1 was often deleted and the percentage of mcr-1-carrying IncI2 plasmids was increased in MCRPEC strains in ***,the antibiotic resistance genes(ARGs),aac(3)-IVa and blaCTX-M-1,emerged and coexisted with mcr-1 in ***,MCRPEC strains in 2019 contained more virulence genes,resulting in an increased proportion of extraintestinal pathogenic ***(ExPEC)strains(36.1%)in MCRPEC strains in 2019 compared to that in 2016(10.5%),implying that these strains could occupy intestinal ecological niches by competing with other commensal *** results suggest that despite the significant reduction in the prevalence of MCRPEC strains in humans from 2016 to 2019,MCRPEC exhibits increased resistance to other clinically important ARGs and contains more virulence genes,which may pose a potential public health threat.

关键词： Colistin mcr-1 Escherichia coli Genomics Human

Dual-network hydrogel based on ionic nano-reservoir for gastric perforation sealing

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Science China Materials 2022年第3期65卷 827-835页

作者： Yuehui Yuan Hao Wu xueyang Ren Jianwu Wang Ruiqing liu Benhui Hu Ning Gu School of Biomedical Engineering and Informatics Nanjing Medical UniversityNanjing 211166China State Key Laboratory of Bioelectronics Jiangsu Key Laboratory for Biomaterials and DevicesSchool of Biological Science and Medical EngineeringSoutheast UniversityNanjing 210009China School of Materials Science and Engineering Nanyang Technological UniversitySingapore 639798Singapore Jiangsu Key Laboratory of Xenotransplantation Nanjing Medical UniversityNanjing 211166China

In recent years,remarkable progress has been made in the research of injectable hydrogel for internal tissue ***,the therapeutic outcome is usually limited when the hydrogel is used for the treatment of gastric perforation due to the high acidic gastric juice and violent deformation of the gastric *** these challenges,we proposed an ionic nano-reservoir(INR)-based dual-network hydrogel,which has excellent adhesion and mechanical properties,and can be easily applied to the perforation site to block the perforation while promoting tissue *** results showed that the first network made of polyacrylamide had cross-linked on the stomach tissue within 5 s under blue light,and enhanced the adhesion performance through mechanical *** nano-hydroxyapatite acted as ionic INR,which can gradually release Ca^(2+) under acid environments to form the second network with sodium alginate and inhibit the swelling of hydrogel in gastric ***,the adhesion was further enhanced through amide covalent bonds at the hydrogel-tissue interface with the presence of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide/N-hydroxysuccinimide(EDC/NHS).The dual network hydrogels obtained by the INR strategy could be employed as a potential therapeutic option for gastric perforation and other similar biomedical prolems.

关键词： injectable hydrogel ionic cross-linking adhesion toughness wound dressing

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冲击荷载下煤岩破碎过程能量释放研究

岩石力学与工程学报 2021年第S02期40卷 3201-3211页

作者：刘晓辉薛洋郑钰桂欣西华大学流体及动力机械教育部重点实验室四川成都610039 西华大学能源与动力工程学院四川成都610039

为探究岩石破碎过程的能量释放特征,利用霍普金森压杆对煤岩展开不同围压和应变率的动态冲击压缩试验,基于SHPB耗散能计算弹性能,讨论煤岩不同初始应力水平和应变率下煤岩的能量释放特征,并进一步探究能量释放对岩石破碎的影响。结果表... 详细信息

为探究岩石破碎过程的能量释放特征,利用霍普金森压杆对煤岩展开不同围压和应变率的动态冲击压缩试验,基于SHPB耗散能计算弹性能,讨论煤岩不同初始应力水平和应变率下煤岩的能量释放特征,并进一步探究能量释放对岩石破碎的影响。结果表明:冲击荷载下,煤岩动态应力–应变曲线基于能量演化过程可明确划分为5个阶段。煤岩具有明显的应变率效应,变形破坏过程存在能量释放滞后效应,弹性能的释放均发生在峰值应力后;随着应变率增大,弹性储能极限呈二次多项式增长,弹性能释放应力呈线性增长;将释放应力进行归一化发现在单轴条件下其变化规律呈线性增长趋势,在三轴条件下呈线性减小趋势,且初始围压越大,应变率依赖性减弱。依托煤岩动态破碎过程中弹性能的释放程度,提出反映岩石破碎效果的能量指标W,其变化规律与煤岩破碎块度呈反比,为破岩效果改善和动力灾害预防提供了一种新的参考手段。

关键词：岩石力学动态应变率煤岩:能量演化能量释放

Antigenicity of tissues and organs from GGTA1/CMAH/β4GalNT2 triple gene knockout pigs

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The Journal of Biomedical Research 2019年第4期33卷 235-243页

作者： Ronggen Wang Miaomiao Ruan Runjie Zhang Lei Chen Xiaoxue Li Bin Fang Chu Li xueyang Ren Jiying liu Qiang Xiong Lining Zhang Yong Jin Lin Li Rongfeng Li Ying Wang Haiyuan Yang Yifan Dai Jiangsu Key Laboratory of Xenotransplantation Key Laboratory of Targeted Intervention of Cardiovascular Disease Collaborative Innovation Center for Cardiovascular Disease Translational Medicine State Key Laboratory of Reproductive Medicine Nanjing Medical UniversityNanjingJiangsu 211166China Shenzhen Xenotransplantation Medical Engineering Research and Development Center Institute of Translational MedicineShenzhen Second People rs HospitalFirst Affiliated Hospital of Shenzhen UniversityShenzhenGuangdong 518035China.

Clinical xenotransplantations have been hampered by human preformed antibody-mediated damage of the *** overcome biological incompatibility between pigs and humans,one strategy is to remove the major antigens[Gal,Neu5 Gc,and Sd(a)]present on pig cells and *** gene(GGTAI,CMAH,and β4 GalNT2)knockout(TKO)pigs were produced in our laboratory by CRISPR-Cas9 *** investigate the antigenicity reduction in the TKO pigs,the expression levels of these three xenoantigens in the cornea,heart,liver,spleen,lung,kidney,and pancreas tissues were *** level of human IgG/IgM binding to those tissues was also investigated,with wildtype pig tissues as *** results showed that aGal,Neu5 Gc,and Sd(a)were markedly positive in all the examined tissues in wildtype pigs but barely detected in TKO *** to wildtype pigs,the liver,spleen,and pancreas of TKO pigs showed comparable levels of human IgG and IgM binding,whereas corneas,heart,lung,and kidney of TKO pigs exhibited significantly reduced human IgG and IgM *** results indicate that the antigenicity of TKO pig is significantly reduced and the remaining xenoantigens on porcine tissues can be eliminated via a gene targeting approach.

关键词： pig xenotransplantation GGTA1 CMAH β4GaINT2 antigenicity

Transmission of Carbapenem Resistance Between Human and Animal NDM-Positive Escherichia coli Strains

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Engineering 2022年第8期8卷 24-33页

作者： Yingbo Shen Fupin Hu Yongqiang Wang Dandan Yin Lu Yang Yiqiang Chen Chunyan Xu Jiyun Li Junyao Jiang xueyang Wang Yulin Fu Dongyan Shao Dejun liu Tengfei Ma Chang Cai Zhangqi Shen Shaolin Wang Juan Li Rong Zhang Yuebin Ke Congming Wu Jianzhong Shen Timothy R.Walsh Yang Wang Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety College of Veterinary MedicineChina Agricultural UniversityBeijing 100193China CAS Key Laboratory of Pathogenic Microbiology and Immunology Institute of MicrobiologyChinese Academy of SciencesBeijing 100101China Institute of Antibiotics&Key Laboratory of Clinical Pharmacology of Antibiotics(MoH) Huashan HospitalFudan UniversityShanghai 200040China Department of Preventive Veterinary Medicine College of Veterinary MedicineChina Agricultural UniversityBeijing 100193China State Key Laboratory of Animal Nutrition College of Animal Science and TechnologyChina Agricultural UniversityBeijing 100193China College of Animal Science and Technology College of Veterinary MedicineZhejiang A and F UniversityHangzhou 311300China State Key Laboratory for Infectious Disease Prevention and Control Collaborative Innovation Center for Diagnosis and Treatment of Infectious DiseaseNational Institute for Communicable Disease Control and PreventionChinese Center for Disease Control and Prevention(CDC)Beijing 102206China The Second Affiliated Hospital of Zhejiang University Zhejiang UniversityHangzhou 310009China Key Laboratory of Genetics&Molecular Medicine of Shenzhen Shenzhen Center for Disease Control and PreventionShenzhen 518055China Department of Zoology University of OxfordOxford OX13SZUK

Although carbapenem use is prohibited in animals in China,carbapenem-resistant Escherichia coli(CREC),especially New Delhi metallo-β-lactamase(NDM)-producing strains,are widely prevalent in foodproducing *** present,the impact of livestock-associated CREC strains on human populations at the national level is ***,we conduct a retrospective cross-sectional study to investigate the prevalence of CREC from clinical settings across 22 Chinese provinces or municipalities and analyze anthropogenic factors associated with their *** also ascertain the blaNDMand blaKPCabundance among pig and chicken farms and present a detailed genomic framework for CREC of animal and human ***,631/29799(2.1%)clinical Escherichia coli(***)isolates were identified as *** analysis revealed that being male,an age below 1,an age between 13 and 18,provinces with greater chicken production,and provinces with higher pig production were associated with higher odds of CREC *** general,73.8%(n=45/61)of pig farms and 62.2%(n=28/45)of chicken farms had a blaNDMabundance of 1×10^(-5)to 1×10^(-3)and 1×10^(-3)to 1×10^(-2),*** all the Chinese NDM-positive ***(n=463)available at the National Center for Biotechnology Information(NCBI),the genomic analysis revealed that blaNDM-5and Inc X3 were the predominant carbapenemase gene-plasmid combination,while a highly homogeneous relationship between NDM-positive isolates from humans and animals was demonstrated at the plasmid and core genome *** the findings suggest frequent CREC transmission between humans and animals,indicating that further discussions on the use of antibiotics in animals and humans are needed,both in China and across the globe.

关键词： Carbapenem resistance Escherichia coli blaNDM-5 One Health Antimicrobial resistance transmission

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基于顾客资源的动态竞争优势

北京工商大学学报（社会科学版） 2006年第6期21卷 21-27,45页

作者：刘建新陈雪阳西南大学经济管理学院重庆400715 涪陵师范学院历史旅游文化学系重庆涪陵400030

随着市场竞争环境变化的加剧,企业战略竞争的基点正在从企业内部的资源转移到企业适应外部环境的动态能力上,顾客资源因能有效地衔接企业内部资源和外部环境机会而成为弥足珍贵的战略性资源,以顾客资源为基础构筑的核心竞争力正日益体... 详细信息

随着市场竞争环境变化的加剧,企业战略竞争的基点正在从企业内部的资源转移到企业适应外部环境的动态能力上,顾客资源因能有效地衔接企业内部资源和外部环境机会而成为弥足珍贵的战略性资源,以顾客资源为基础构筑的核心竞争力正日益体现出强大的动态竞争优势。因此,企业只有通过加快转变企业的战略管理理念、重构企业内部的业务流程、改革传统的价值链模式和实施企业的顾客忠诚化管理等途径构筑基于顾客资源的动态竞争优势,才能将企业立于竞争的不败之地。

关键词：顾客资源顾客忠诚动态能力竞争优势

硬质地基上干坞坞口模袋砂围堰变形空间效应及其方案优选

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隧道建设 2017年第A1期37卷 20-25页

作者：刘力英黄雪阳广州市市政工程设计研究总院广东广州510060

为了探索硬质地基上干坞坞口模袋砂围堰变形的空间效应及合理的平面布置方案,以广州洲头咀沉管隧道轴线干坞坞口模袋砂围堰工程为背景,借助数值手段建立围堰三维有限元模型,明确了干坞内降水工况下围堰空间条件下的水平向变形规律,并结... 详细信息

为了探索硬质地基上干坞坞口模袋砂围堰变形的空间效应及合理的平面布置方案,以广州洲头咀沉管隧道轴线干坞坞口模袋砂围堰工程为背景,借助数值手段建立围堰三维有限元模型,明确了干坞内降水工况下围堰空间条件下的水平向变形规律,并结合现场实测数据进行了验证,同时对多折角U形和两直角U形2种围堰平面布置方案进行了对比。结果表明:1)降水工况下,模袋砂围堰纵向不同位置的水平向变形规律不同,将围堰变形视为平面应变问题存在缺陷;2)围堰存在类似于基坑的空间效应,其本质源于围堰自身平面布置导致的系统刚度差异;3)围堰顶部斜裂缝产生的原因是围堰角部与中部系统刚度不同,差异变形的存在导致了裂缝的发育;4)多折角U形围堰布置方案优于两直角U形布置方案,前者对空间效应的利用更为充分。

关键词：沉管隧道硬质地基模袋砂围堰空间效应数值模拟