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High-Throughput MicroRNA and mRNA Sequencing Reveals that MicroRNAs May Be Involved in Pectinesterase-Mediated Cold Resistance in Potato

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Phyton-International Journal of Experimental Botany 2020年第3期89卷 561-586页

作者： Chongchong Yan Qianqian Wang Nan Zhang Jiajia Wang xuexiang ren Bingjie Xue Xiaojing Pu Zhihong Xu Huajun Liao Anhui Academy of Agricultural Sciences Hefei230031China Anhui Vocational College of City Management Hefei231635China Funan County Agricultural Technology Promotion Center Funan236300China Jieshou County Agricultural Technology Promotion Center Jieshou236500China

Since potato cultivars are sensitive to low temperature,cold injury severely affects the geographical distribution and yield of *** some miRNAs have been identified in response to cold stress in plants,there is no report about the role of miRNAs in the response to cold stress in ***,via high throughput sequencing,we described the profiling of cold stress response to miRNA and mRNA in *** small RNA and six mRNA libraries were constructed and sequenced.296 known and 211 novel miRNAs were identified,in which 34 miRNAs in Cold Group(CG)had the higher expression quantity than which in Normal Group(NG)and 32 in CG had lower expression quantity than which in NG.3068 differentially expressed genes were detected between NG and CG,in which 1400 genes were up-regulated and 1668 genes were *** metabolism pathway of starch and sucrose(ko00500)is the common KEGG pathway in differentially expressed miRNA and *** this pathway,StuPME21575 and StuPME42971 are pectinesterase which mainly catalyzes the pectin-forming pectate,which are controlled by stu-miR6023 and *** the potato suffering cold stress,these two miRNAs expression levels became higher,but their target genes expression levels were just opposite and this result is the same with qRT-PCR.

关键词： Potato microRNA RNA-Seq low-temperature stress pectinesterase

The Pythium periplocum elicitin PpEli2 confers broad-spectrum disease resistance by triggering a novel receptor-dependent immune pathway in plants

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Horticulture Research 2023年第2期10卷 41-52页

作者： Kun Yang Yi Wang Jialu Li Yaxin Du Ying Zhai Dong Liang Danyu Shen Rui Ji xuexiang ren Hao Peng Maofeng Jing Daolong Dou Key Laboratory of Biological Interaction and Crop Health Department of Plant PathologyNanjing Agricultural UniversityNanjing 210095China Department of Plant Pathology Washington State UniversityPullmanWA 99164USA Institute of Plant Protection Jiangsu Academy of Agricultural SciencesNanjing 210014China Institute of Plant Protection and Agro-Products Safety Anhui Academy of Agricultural SciencesHefei 230001China Academy for Advanced Interdisciplinary Studies Nanjing Agricultural UniversityNanjing 210095China

Elicitins are microbe-associated molecular patterns produced by oomycetes to elicit plant *** is still unclear whether elicitins derived from non-pathogenic oomycetes can be used as bioactive molecules for disease ***,for the first time we identify and characterize an elicitin named PpEli2 from the soil-borne oomycete Pythium periplocum,which is a non-pathogenic mycoparasite colonizing the root ecosystem of diverse plant *** by a novel cell surface receptor-like protein,REli,that is conserved in various plants(***,pepper,soybean),PpEli2 can induce hypersensitive response cell death and an immunity response in Nicotiana ***,PpEli2 enhances the interaction between REli and its co-receptor *** receptor-dependent immune response triggered by PpEli2 is able to protect various plant species against Phytophthora and fungal ***,our work reveals the potential agricultural application of non-pathogenic elicitins and their receptors in conferring broad-spectrum resistance for plant protection.

关键词： elicitin soybean soil

The Post-exposure Effects of Spinosad and Plant Allelochemicals on Biological Characteristics of Helicoverpa armigera(Lepidoptera： Noctuvidae)

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Agricultural Science & Technology 2016年第2期17卷 391-393页

作者： xuexiang ren Zhenghe YE Xianyan SU Dong WANG Shanmin CHENG Zhongchen FAN Institude of Plant Protection and Agro-Products Safety Anhui Academy of Agricultural Science Hefei 230031 China Jinan Center For Disease Control and Prevention Jinan 250100 China Anhui Hui Fu Qiang Agricultural Science and Technology Co. Ltd Hefei 230022 China

In order to define the combined effects of spinosad and plant allelochemicals on Helicoverpa armigera(Lepidoptera:Noctuvidae), the research fed them on artificial diets with different doses of spinosad, gossypol, quercetin and tannicacid, and the changes of pupal and imaginal biological characteristics were observed and recorded. The results showed that spinosad at sublethal concentrations decreased pupation ratio and pupal weight, prolonged prepupal and pupal periods, and decreased emergence ratio, fecundity and longevity of adults. Combined with plant allelochemicals, spinosad significantly prolonged pupal period and shorten adult longevity. Other life-cycle parameters such as pupation ratio, pupal weights, emergence ratio, eggs laid per female and hatch ratio did not change significantly. The results clearly indicated that plant allelochemicals did not change spinosad efficiency on H. armigera, but the combination of spinosad and plant allelochemicals might affect pest population dynamics significantly by changing its development.

关键词： exposure Lepidoptera emergence larvae quercetin prolonged clearly shorten toxicity weights

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药剂混配对B型烟粉虱药效及烟草安全性评价

中国烟草科学 2011年第2期32卷 48-51,65页

作者：任学祥王东王杰王开运山东农业大学植物保护学院山东泰安271018 济南市疾病预防控制中心济南272033

利用试管药膜法测定了啶虫脒等药剂及其混配对B型烟粉虱成虫的毒力,利用烟草植株测定了哒螨灵、啶虫脒及混配对B型烟粉虱的药效以及对烟草植株的安全性。结果显示,啶虫脒在3种新烟碱类药剂中显示了较高毒力,LC50为7.119 5 mg/L,哒螨灵... 详细信息

利用试管药膜法测定了啶虫脒等药剂及其混配对B型烟粉虱成虫的毒力,利用烟草植株测定了哒螨灵、啶虫脒及混配对B型烟粉虱的药效以及对烟草植株的安全性。结果显示,啶虫脒在3种新烟碱类药剂中显示了较高毒力,LC50为7.119 5 mg/L,哒螨灵与啶虫脒1:1与2:1混配显示了一定的拮抗作用,4:1与8:1混配显示出了一定的相加作用,LC50分别为1.610 3和1.145 2 mg/L;啶虫咪和毒死蜱1:4与1:8混配的LC50分别为21.813和25.730 mg/L,共毒系数分别为135.68和177.16,有较高的增效作用。药效试验结果显示,15%哒螨灵EC与5%啶虫脒EC质量比8:1混配稀释1 000倍液对烟粉虱显示了优异的防效,3～10 d防效在89.6%～95.8%。安全性试验显示,15%哒螨灵EC与5%啶虫脒EC质量比8:1混配稀释1 000倍液对部分烟草植株可能产生了轻微药害,但不影响其生长。

关键词： B型烟粉虱哒螨灵啶虫脒药效安全性

Study on the Relationship of Spray Rate to Air Pressure and Gravity Drop of Siphonic Nozzle

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Agricultural Science & Technology 2017年第10期18卷 1960-1962页

作者： Xianyan SU xuexiang ren Weiwei HE Fei HU Zhenghe YE Institute of Plant Protection and Agricultural Product Quality Safety Anhui Academy of Agricultural Sciences Hefei 230031 China Anhui Agriculture University Hefei 230001 China

In order to further clarify the spraying performance of siphonic nozzle, the spray rate of siponic cone nozzle with aperture of 1.0 mm was determined at 15 different air pressure levels and 12 gravity drop levels, and DPS and SPSS were used to make the difference analysis and modeling, which clarified the relationship between the spray rate of the siphonic cone nozzle with aperture of 1.0 mm and air pressure and gravity drop, getting the regression equation of Y=406.854P＋ 1.904G＋77.524. The study could provide a theoretical basis for the optimization and improvement of plant protection spraying equipment.

关键词： Siphonic nozzle Spray rate Air pressure Gravity drop

虫螨腈胁迫对甜菜夜蛾保护酶系和解毒酶系的诱导效应

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植物保护学报 2009年第5期36卷 455-460页

作者：张文成王开运牛芳王东任学祥山东农业大学植物保护学院泰安271018

为探明虫螨腈对甜菜夜蛾幼虫体内生化代谢的影响及酶抑制剂对虫螨腈的增效潜力,采用点滴法测定了虫螨腈对甜菜夜蛾3、4、5龄幼虫的毒力和顺丁烯二酸二乙酯(DEM)、增效醚(PBO)、磷酸三苯酯(TPP)对虫螨腈的增效作用,并采用生化分析法测定... 详细信息

为探明虫螨腈对甜菜夜蛾幼虫体内生化代谢的影响及酶抑制剂对虫螨腈的增效潜力,采用点滴法测定了虫螨腈对甜菜夜蛾3、4、5龄幼虫的毒力和顺丁烯二酸二乙酯(DEM)、增效醚(PBO)、磷酸三苯酯(TPP)对虫螨腈的增效作用,并采用生化分析法测定了LD25和LD50剂量虫螨腈对甜菜夜蛾体内保护酶系和解毒酶系的影响。结果表明,虫螨腈对甜菜夜蛾不同龄期幼虫的毒力差异不大,处理后24h和48h的LD50基本相同。PBO对虫螨腈有拮抗作用,增效比为0.21,而DEM和TPP对虫螨腈无增效作用。虫螨腈对甜菜夜蛾幼虫体内超氧化物歧化酶具有激活作用,对过氧化氢酶、过氧化物歧化酶及羧酸酯酶具有先抑制后诱导的作用;虫螨腈处理后6～24h,谷胱甘肽-S-转移酶活性显著提高,最高为对照的1.85倍;处理6h后,甜菜夜蛾体内细胞色素P450含量明显下降,而12～24h后,又明显上升,最高时为对照的1.86倍。

关键词：虫螨腈甜菜夜蛾增效剂保护酶解毒酶

含OH11发酵液玉米种衣剂的研制及其对茎基腐病的效果评价

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中国生物防治学报 2022年第6期38卷 1566-1571页

作者：任学祥苏贤岩闫学梅迟雨李钊叶正和钱国良安徽省农业科学院植物保护与农产品质量安全研究所合肥230031 山东省安丘市农业农村局安丘262100 不详

通过正交试验,筛选分散剂、乳化剂及增稠剂,利用湿法砂磨法,将OH11发酵液研制成30%悬浮种衣剂,将研制的玉米种衣剂不同药种比包衣后测定对玉米根长、苗长、鲜重指标影响,并通过菌土混合法,测定了药剂对玉米茎基腐防效。结果显示,30%OH1... 详细信息

通过正交试验,筛选分散剂、乳化剂及增稠剂,利用湿法砂磨法,将OH11发酵液研制成30%悬浮种衣剂,将研制的玉米种衣剂不同药种比包衣后测定对玉米根长、苗长、鲜重指标影响,并通过菌土混合法,测定了药剂对玉米茎基腐防效。结果显示,30%OH11发酵液悬浮种衣剂最佳配方为OH11发酵液30%、木钠0.8%、PEG40003%、XG0.2%、红染4%、成膜剂3%,水余量。该种衣剂药种比1:200、1:500包衣对玉米生长发育影响不大,1:200时对玉米茎基腐(腐霉菌)防效达93.55%,1:500防效达80.51%,显著高于对照药剂30%戊唑醇悬浮种衣剂(39.16%)。结果显示30%OH11发酵液悬浮种衣剂能够很好的应用于玉米茎基腐(腐霉菌)病的防治。

关键词： OH11发酵液玉米种衣剂茎基腐病腐霉菌

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吡蚜酮等对烟粉虱的毒力测定和药效试验

农药科学与管理 2010年第1期31卷 49-51页

作者：任学祥张晓芳牛芳山东农业大学植物保护学院山东泰安271018

本实验测定了吡蚜酮、吡虫啉、毒死蜱、吡丙醚4种药剂对烟粉虱成虫的室内毒力和田间药效,室内毒力测定结果显示:吡蚜酮、吡虫啉、毒死蜱、吡丙醚对烟粉虱成虫的LC50值分别为24.10、12.13、140.41、1211.5mg/L,相对于吡丙醚而言,吡蚜酮... 详细信息

本实验测定了吡蚜酮、吡虫啉、毒死蜱、吡丙醚4种药剂对烟粉虱成虫的室内毒力和田间药效,室内毒力测定结果显示:吡蚜酮、吡虫啉、毒死蜱、吡丙醚对烟粉虱成虫的LC50值分别为24.10、12.13、140.41、1211.5mg/L,相对于吡丙醚而言,吡蚜酮、吡虫啉、毒死蜱的毒力倍数为50.3,99.9,8.6。药效试验结果显示,在4种药剂中,毒死蜱和吡虫啉的药效明显的高于吡丙醚、吡蚜酮,吡丙醚的药效最差;显示毒死蜱的持效期短于吡虫啉,而新型杀虫剂吡蚜酮并没有显示良好的防效。

关键词：烟粉虱吡蚜酮毒力测定药效试验

哒螨灵对三种害虫的毒力比较及其应用潜力评价

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植物保护学报 2011年第1期38卷 65-69页

作者：任学祥王开运左一鸣肖昌海杨焕青山东农业大学植物保护学院泰安271018 山东泰安出入境检验检疫局泰安271000 山东省联合农药工业有限公司济南250100

为明确杀螨剂哒螨灵对B型烟粉虱、水稻灰飞虱和黄曲条跳甲等3种害虫的毒力和扩大其使用范围,采用玻管药膜法测定了哒螨灵等药剂对B型烟粉虱和水稻灰飞虱成虫的毒力,用浸渍法测定了哒螨灵等对B型烟粉虱若虫和黄曲条跳甲的毒力。结果显示... 详细信息

为明确杀螨剂哒螨灵对B型烟粉虱、水稻灰飞虱和黄曲条跳甲等3种害虫的毒力和扩大其使用范围,采用玻管药膜法测定了哒螨灵等药剂对B型烟粉虱和水稻灰飞虱成虫的毒力,用浸渍法测定了哒螨灵等对B型烟粉虱若虫和黄曲条跳甲的毒力。结果显示,哒螨灵对烟粉虱成虫和水稻灰飞虱成虫的毒力最高,触杀LC50分别为0.952mg/L和0.252mg/L;哒螨灵对B型烟粉虱若虫的触杀LC50为43.148mg/L,其毒力较啶虫脒和吡虫啉低,与烯啶虫胺毒力相当,但较吡丙醚毒力高近1倍;哒螨灵对黄曲条跳甲的LC50为195.123mg/L,其毒力低于毒死蜱,但高于啶虫脒和吡虫啉。表明哒螨灵不仅对B型烟粉虱和水稻灰飞虱成虫毒力高,对烟粉虱若虫和黄曲条跳甲的毒力也较高,有进一步开发应用的潜力。

关键词：哒螨灵 B型烟粉虱水稻灰飞虱黄曲条跳甲毒力