检索结果-南通市图书馆

Mechanism of Deca-BDE-induced apoptosis in Neuro-2a cells:Role of death-receptor pathway and reactive oxygen species-mediated mitochondrial pathway

引用

Journal of Environmental Sciences 2016年第8期28卷 241-251页

作者： Hongmei Chen xuexi tang Bin Zhou Ningning Xu You Wang Department of Marine Ecology College of Marine Life Sciences Ocean University of China

Decabromodiphenyl ether（BDE-209） is a prevalent polybrominated diphenyl ether（PBDE）congener known to have neurotoxicity. Effects of BDE-209 on Neuro-2a cells were performed in the present study and the possible apoptotic pathway was discussed. Results indicated that BDE-209 induced Neuro-2a cell apoptosis, increased the protein expression of Fas and Fas-associated death domain-containing protein（FADD） and activated the caspase-8 and-3activities in a concentration-dependent manner, inferring the death-receptor pathway was involved in the apoptotic process. Meanwhile, BDE-209 exposure increased the Bax/Bcl-2 ratio and decreased the cellular mitochondrial membrane potential（MMP） which led to cytochrome C released to the cytoplasm. The intracellular caspase-9 was elevated simultaneously,which caused downstream caspase cascade and triggered cell apoptosis. Moreover, BDE-209 exposure increased cellular reactive oxygen species（ROS） level in a concentration-dependent manner and the addition of N-acetyl-L-cysteine（NAC）, known as ROS scavengers, obviously reduced the apoptotic rate and a positive relationship was observed between the degree of apoptosis blocking and the loss of MMP and ROS production. We thus concluded that BDE-209 induced Neuro-2a cell apoptosis via the combination of the death-receptor signaling pathway and the mitochondrial signaling pathway. The elevated ROS production was considered to magnify the intracellular apoptosis signal and played a crucial role in apoptosis of Neuro-2a cells induced by BDE-209.

关键词： Decabromodiphenyl ether(BDE-209) Apoptosis Reactive oxygen species(ROS) Neuro-2a cells

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Allelopathic growth inhibition of Heterosigma akashiwo by the three Ulva spcieces(Ulva Pertusa,Ulva Linza,Enteromorpha intestinalis) under laboratory conditions

引用

Acta Oceanologica Sinica 2012年第3期31卷 138-144页

作者： WANG Renjun FENG Lei tang xuexi WANG Jinhe DONG Shuanglin College of Life Science Qufu Normal UniversityQufu 273165China Marine Ecology Laboratory of Marine Life College Ocean University of ChinaQingdao 266003China Department of Agricultural Engineering Dongying Vocation CollegeDongying 257091China Dongying City Ocean and Fishery Bureau Dongying 257091China

Allelopathic effects of several concentrations of fresh tissue,dry powder and dry tissue of three bloom-forming green macroalgae Ulva pertusa,Ulva linza and Enteromopha intestinalis on the red tide microalga Heterosigma akashiwo were evaluated in microcosms *** effects of macroalgae culture medium filtrate were investigated on *** using initial or semi-continuous filtrate *** studies on the algicidal effects of one aqueous and four organic solvent extracts from the macroalgae on the microalga were carried out to confirm the existence of allelochemicals in the tissue of these *** dry powder of *** was extracted with methanol,and the methanol extracts were partitioned to petroleum ether phase,ethyl acetate phase,butanol phase and distilled phase by liquid-liquid *** bioassays of the activity of every fraction were carried out on *** resultant microcosms assay showed that the growth of *** was strongly inhibited by using fresh tissues,dry powder or dry tissue of these three macroalgae,while aqueous and methanol extracts of both macroalgae had strong inhibitory effects on the growth of ***,and the EC50 values for methanol extract of ***,*** or *** were 0.016,0.028× 10-12 or 0.033× 10-12,*** the other three organic solvent extracts (acetone,ether and chloroform) had no apparent effect on its growth,this suggests that the allelochemicals from these three maeroalgae had relatively high *** activity of petroleum ether phase,ethyl acetate phase,butanol phase and distilled phase of *** methanol extract was carried out on *** indicating that petroleum ether phase and ethyl acetate phase had stronger algicidal effect on *** inhibition effect of the ethyl acetate phase was not as strong as that of petroleum ether phase,and effective concentration of petroleum ether phase was 17 mg/L for ***,no significant al

关键词： allelopathy green tide macroalgae red tide microalga extracts

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Bacterial community succession in response to dissolved organic matter released from live jellyfish

引用

Journal of Oceanology and Limnology 2019年第4期37卷 1229-1244页

作者： HAO Wenjin WICHELS Antje FUCHS Bernhardt tang xuexi GERDTS Gunnar School of Life Science Nantong University Nantong 226019 China Department of Marine Ecology College of Marine Life Sciences Ocean University of China Qingdao 266000 China Alfred Wegener Institute for Polar and Marine Research Biologische Anstalt Helgoland Helgoland 27498 Germany Max-Planck-Institute for Marine Microbiology Department Molecular Ecology Celsiusstr. 1 Bremen 28359 Germany

Jellyfish blooms have increased worldwide, and the outbreaks of jellyfish population not only affect the food web structures via voracious predation but also play an important role in the dynamics of nutrients and oxygen in planktonic food webs. However, it remains unclear whether specific carbon compounds released through jellyfish metabolic processes have the potential to shape bacterial community composition. Therefore, in this study, we aimed to investigate the compositional succession of the bacterioplankton community in response to the dissolved organic matter (DOM) released by the live Scyphomedusae Cyanea lamarckii and Chrysaora hysoscella collected from Helgoland Roads of the North Sea. The bacterial community was significantly stimulated by the DOM released form live jellyfish and different dominant phylotypes were observed for these two Scyphomedusae species. Furthermore, the bacterial community structures in the different DOM sources, jellyfish-incubated media, Kabeltonne seawater, and artificial seawater (DOM-free) were significantly different, as revealed by automated ribosomal intergenic spacer analysis fingerprints. Catalyzed reporter deposition fluorescence in situ hybridization (CARD-FISH) revealed a rapid species-specific shift in bacterial community composition. Gammaproteobacteria dominated the community instead of the Bacteroidetes community for C. lamarckii, whereas Gammaproteobacteria and Bacteroidetes dominated the community for C. hysoscella. The significant differences in the bacterial community composition and succession indicate that the components of the DOM released by jellyfish might differ with jellyfish species.

关键词： bacterial community structure bacterial community composition dissolved organic matter jellyfish automated rRNA intergenic spacer analysis (ARISA) catalyzed reporter deposition fluorescence in situ hybridization (CARD-FISH)

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Stable isotopic composition of otoliths in identification of stock structure of small yellow croaker(Larimichthys polyactis) in China

引用

Acta Oceanologica Sinica 2016年第6期35卷 29-33页

作者： WANG Yukun HUANG Jiansheng tang xuexi JIN Xianshi SUN Yao Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences Laboratory of Ecology College of Marine Life SciencesOcean University of China

Oxygen and carbon isotope ratios（δ^（18）O and δ^（13）C） in otoliths were used to identify the stock structure of small yellow croaker,Larimichthys *** were collected from fish at five locations across the Yellow Sea and the Bohai Sea representing most of their distributional range and fisheries *** significant differences in the isotopic signatures showed that the five locations could be chemically distinguished and clearly separated,indicating stock *** of δ^（18）O and δ^（13）C values suggested that population of *** could be divided into the Bohai Sea group,the southern Yellow Sea group and the central Yellow Sea *** analysis of δ^（18）O and δ^（13）C values demonstrated a high significant difference with 85.7% classification *** spatial separation of *** indicated a complex stock structure across the Yellow Sea and the Bohai *** results indicate that optimal fisheries management may require a comprehensive consideration on the current spatial *** study has provided further evidence that measurement of the stable isotopes ratios in otolith can be a valuable tool in the delineation of fishery management units.

关键词： small yellow croaker otolith stable isotope chemistry stock structure

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

River Flow Control on the Phytoplankton Dynamics of Chesapeake Bay

引用

Journal of Ocean University of China 2013年第1期12卷 103-114页

作者： YU Qingyun WANG You tang xuexi LI Ming Laboratory of Ecology College of Marine Life Sciences Ocean University of China Horn Point Laboratory Center for Environmental Science University of Maryland

Recent observations support an emerging paradigm that climate variability dominates nutrient enrichment in costal eco-systems, which can explain seasonal and inter-annual variability of phytoplankton community composition, biomass (Chl-a), and primary production (PP). In this paper, we combined observation and modeling to investigate the regulation of phytoplankton dynamics in Chesapeake Bay. The year we chose is 1996 that has high river runoff and is usually called a 'wet year'. A 3-D physical-biogeochemical model based on ROMS was developed to simulate the seasonal cycle and the regional distributions of phytoplankton biomass and primary production in Chesapeake Bay. Based on the model results, NO3 presents a strong contrast to the river nitrate load during spring and the highest concentration in the bay reaches around 80 mmol Nm-3 . Compared with the normal year, phytoplankton bloom in spring of 1996 appears in lower latitudes with a higher concentration. Quantitative comparison between the modeled and observed seasonal averaged dissolved inorganic nitrogen concentrations shows that the model produces reliable results. The correlation coefficient r2 for all quantities exceeds 0.95, and the skill parameter for the four seasons is all above 0.95.

关键词：浮游植物生物量切萨皮克湾动力学流量控制模型模拟沿海生态系统生物地球化学初级生产

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Photosynthetic response of floating Ulva prolifera to diurnal changes of in-situ environments on the sea surface

引用

Journal of Oceanology and Limnology 2019年第2期37卷 589-599页

作者： ZHAO Xinyu tang xuexi HU Shunxin ZHONG Yi QU Tongfei WANG Ying College of Marine Life Sciences Ocean University of China Qingdao 266003 China Laboratory for Marine Ecology and Environmental Science Qingdao National Laboratory for Marine Science and Technology Qingdao 266071 China

During yellow sea green tide outbreak, the thalli of Ulva prolifera drifted more than 350 km on the sea surface from off shore of Jiangsu Province to Qingdao city, where they were exposed to complex changes of local environments. The purpose of this study is to investigate the response of the thalli to diurnal changes of environments on the sea surface, and a sea surface environment simulator (SSES) was designed to simulate the natural environment of floating U . prolifera mat. A control experiment is designed in the laboratory, which was processed under suitable conditions (20°C for temperature, 72 μmol photons/(m 2 ·s) for light intensity and 30 for salinity), and an in-situ research was conducted to study the photosynthetic responses of floating U . prolifera to diurnal changes of environments. The results show that the photosynthetic efficiency of the thalli decreased gradually with time, and decreased rapidly after 14:00 local time (LT). After exposed to the environment on the sea surface for 6 h, the photosynthetic activity of the thalli decreased significantly. Furthermore, physiological-level and molecular-level experiments revealed that non-photochemical quenching (NPQ), cyclic electron flow (CEF) and energy redistribution between PSI and PSII all played an important role in the strong photosynthetic plasticity of U . prolifera . NPQ is the most important photoprotective responses to environmental changes before 12:00 LT. The results also confirmed that the CEF and energy redistribution between PSI and PSII are the main synergistic eff ects for the thalli to adapt to the environmental changes when the process NPQ cannot work. And the result can further reveal the reason why U . prolifera can adapt to the living condition of long distance drift on the sea surface. The findings of this research could provide a theoretical basis for explaining outbreaks of the green tide and instructing the management of the problem.

关键词： photosynthetic system floating Ulva prolifera diurnal change photosynthetic plasticity

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Interaction between Chlorella vulgaris and bacteria:interference and resource competition

引用

Acta Oceanologica Sinica 2014年第1期33卷 135-140页

作者： QU Liang WANG Renjun ZHAO Peng CHEN Ruinan ZHOU Wenli tang Liuqing tang xuexi Ocean University of China College of Life Science Qufu Normal University Department of Marine Economy National Marine Data and Information Service Tianjin Agricultural University Tianjin Key Laboratory of Aqua-ecology and Aquaculture

Research of interaction mechanism between Chlorella vulgaris and two bacterial strains（Z-QD08 and Z-QS01） were conducted under laboratory *** rates of bacteria and *** were tested under co-culture conditions to evaluate the effects of concentrations of *** and bacteria on their *** test whether the availability of inorganic nutrients,vitamins and trace metals affects the interactions between *** and bacteria,experiments were performed with or without the culture medium filtrate of *** or *** results showed that the growth of *** was promoted at low concentrations of bacteria（5×106cells/ml）,and expressed a positive correlation with the bacteria density,whereas opposite trend was observed for treatments with high bacteria density（10×106cells/ml and 20×106cells/ml）.The growth rate of bacteria decreased with the increasing concentrations of *** growth of bacteria Z-QD08 was inhibited by *** through interference competition,while the mechanism for interaction between bacteria Z-QS01 and *** was resource *** influence of cell density on the interaction between microalgae and bacteria was also *** experiments confirm some elements of published theory on interactions between heterotrophic bacteria and microalgae and suggest that heterotrophic bacteria play an important role in the development of blooms in natural waters.

关键词： Chlorella vulgaris bacteria Z-QD08 bacteria Z-QS01 interaction mechanism

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Investigation of the decline of Ulva prolifera in the Subei Shoal and Qingdao based on physiological changes

引用

Journal of Oceanology and Limnology 2021年第3期39卷 918-927页

作者： Ya HAO Chen GUAN Xinyu ZHAO Tongfei QU xuexi tang Ying WANG College of Marine Life Sciences Ocean University of ChinaQingdao 266100China Laboratory for Marine Ecology and Environmental Science Qingdao National Laboratory for Marine Science and TechnologyQingdao 266071China

It has been a scientific consensus that Ulva prolifera green tide that break out annually between May and August in the recent decade off eastern coast of Yellow *** investigate the ecological mechanism of the decline of *** green tide,we compared the physiological characteristics of *** samples collected in three stages of the green tide in Subei Shoal north Jiangsu,the initiation point,on May 10(early,the initiation),July 10(middle,the outbreak),and in Taipingjiao,Qingdao on August 10(late,the decline).Compared to the early samples,the middle,and late samples showed a lower chlorophyll content and photosynthetic e fficiency at a higher lipid peroxidation *** middle and later samples had fewer chloroplasts in the cells of *** than those in the early *** addition,the cells of *** sampled in the late stage showed ruptured and deformed cell *** in physiological function were reflected in the growth *** the middle and late *** samples presented negative growth *** analysis results show that the rising temperature was mainly responsible for the local decline of *** green *** high light-intensity that could induce the photoinhibition was also an important factor for the ***,*** population that remained in the Subei Shoal and those that northward drifted to Qingdao experienced the same decline processes:rising temperature and continuous high light conditions in the late phase of green tide reduced the photosynthetic capacity and destroyed the antioxidant system of the thallus,which lead to decrease of *** only difference is that the temperature in the Subei Shoal rose earlier than that in Qingdao;thus,*** remaining in the Subei Shoal went disappeared first.

关键词： Ulva prolifera green tide decline process photoinhibition lipid peroxidation

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Effects of two polybrominated diphenyl ethers(BDE-47,BDE-209) on the swimming behavior, population growth and reproduction of the rotifer Brachionus plicatilis

引用

Journal of Environmental Sciences 2015年第2期27卷 54-63页

作者： Jingjing Sha You Wang Jianxia Lv Hong Wang Hongmei Chen Leilei Qi xuexi tang Department of Marine Ecology College of Marine Life Sciences Ocean University of China Thermo Fisher Scientific (Beijing) Co. Ltd.

Polybrominated diphenyl ethers（PBDEs） are new kinds of persistent organic pollutants（POPs） and their potential threats to the equilibrium and sustainability of marine ecosystems have raised worldwide concerns. Here, two kinds of PBDEs, tetra-BDE（BDE-47）and deca-BDE（BDE-209） were applied, and their toxic effects on the swimming behavior,population growth and reproduction of Brachionus plicatilis were investigated. The results showed that：（1） The actual concentrations of BDE-47 and-209 in the seawater phase measured by GC–MS（Gas Chromatography-Mass Spectrometer） were much lower than their nominal concentrations.（2） In accordance with the 24-hr acute tests, BDE-209 did not show any obvious swimming inhibition to rotifers, but a good correlation did exist between the swimming inhibition rate and BDE-47 concentration suggesting that BDE-47 is more toxic than BDE-209.（3） Both BDE-47 and-209 had a significant influence on the population growth and reproduction parameters of B. plicatilis including the population growth rate, the ratio of ovigerous females/non-ovigerous females（OF/NOF）, the ratio of mictic females/amictic females（MF/AF）, resting egg production and the mictic rate, which indicate that these parameters in B. plicatilis population were suitable for monitoring and assessing PBDEs. Our results suggest that BDE-47 and-209 are not acute lethal toxicants and may pose a low risk to marine rotifers at environmental concentrations for short-term exposure. They also accumulate differently into rotifers. Further research data are needed to understand the mechanisms responsible for the effects caused by PBDEs and to assess their risks accurately.

关键词： BDE-47 BDE-209 Brachionus plicatilis Swimming inhibition Population growth Reproductive toxicity

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Bacterial communities fluctuate in abundance and diversity under simulated oil-contaminated seawater conditions

引用

Journal of Oceanology and Limnology 2019年第2期37卷 615-627页

作者： LI Xiaohong YOU Cai QU Liang ZHOU Bin tang xuexi XIAO Hui College of Marine Life Sciences Ocean University of China Qingdao 266003 China Laboratory for Marine Ecology and Environmental Science Qingdao National Laboratory for Marine Science and Technology Qingdao 266071 China Institute of Oceanology Chinese Academy of Sciences Qingdao 266071 China CNOOC Limited Tianjin Branch Tianjin 300459 China

Marine bacteria have recently been identified as a potent solution for petroleum hydrocarbon degradation in response to hazardous oceanic oil spills. In this study, a mesocosm experiment simulating a petroleum spill event was performed to investigate changes in the abundance, structure, and productivity of bacterial communities in response to oil pollution. Cultured heterotrophic bacteria and total bacteria showed a consistent trend involving an immediate decrease in abundance, followed by a slight increase, and a steady low-level thereafter. However, the changing trend of bacterial productivity based on bacterial biomass and bacterial volume showed the opposite trend. In addition, the density of oil-degrading bacteria increased initially, then subsequently declined. The change in the bacterial community structure at day 0 and day 28 were also analyzed by amplified ribosomal DNA restriction analysis (ARDRA), which indicated that the species diversity of the bacterial community changed greatly after oil pollution. Alphaproteobacteria (40.98%) replaced Epsilonproteobacteria (51.10%) as the most abundant class, and Gammaproteobacteria (38.80%) became the second most dominant class in the whole bacterial community. The bacterial communities in oil-contaminated seawater (32 genera) became much more complex than those found in the natural seawater sample (16 genera). The proportion of petroleum-degrading bacteria in the oil-contaminated seawater also increased. In this study, culture-dependent and culture-independent approaches were combined to elucidate changes in both bacterial productivity and community structure. These findings will contribute to a better understanding of the role that bacteria play in material cycling and degradation in response to oil pollution.

关键词： petroleum pollution bacterial community bacterial growth amplified ribosomal DNA restriction analysis (ARDRA)

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：