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Directed hepatic differentiation from embryonic stem cells

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Protein & Cell 2011年第3期2卷 180-188页

作者： xuesong chen Fanyi Zeng Laboratory of Developmental Biology Institute of Medical ScienceShanghai Jiao Tong University School of MedicineShanghai 200025China Shanghai Institute of Medical Genetics Shanghai Jiao Tong University School of MedicineShanghai

The liver is the largest internal organ in mammals,and is important for the maintenance of normal physiological functions of other tissues and organs.Hepatitis,cirrhosis,liver cancer and other chronic liver diseases are serious threats to human health,and these problems are compounded by a scarcity of liver donors for transplantation therapies.Directed differentiation of embryonic stem cells to liver cells is a promising strategy for obtaining hepatocytes that can be used for cell transplantation.In vitro hepatocyte differentiation of embryonic stem cells requires a profound understanding of normal development during embryonic hepatogenesis.Here we provide a simple description of hepatogenesis in vivo and discuss directed differentiation of embryonic stem cells into hepatocytes in vitro.

关键词： stem cell differentiation liver development signaling pathway

Cytoplasmic YAP1-mediated ESCRT-III assembly promotes autophagic cell death and is ubiquitinated by NEDD4L in breast cancer

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Cancer Communications 2023年第5期43卷 582-612页

作者： Yan Guo Yuqing Cui Yangyang Li Xiaoying Jin Dandan Wang Mengxia Lei Fengzhi chen Yali Liu Jinwen Xu Guanyu Yao Guangchun Zeng xuesong chen Department of Breast Medical Oncology Harbin Medical University Cancer HospitalHarbinHeilongjiangP.R.China Department of Pathology Harbin Medical University Cancer HospitalHarbinHeilongjiangP.R.China

Background:Nuclear Yes1-associated transcriptional regulator(YAP1)promotes tumor progression.However,the function of cytoplasmic YAP1 in breast cancer cells and its impact on the survival of breast cancer patients remain unclear.Our research aimed to explore the biological function of cytoplasmic YAP1 in breast cancer cells and the possibility of cytoplasmic YAP1 as a predictive marker of breast cancer survival.Methods:We constructed cell mutant models,including NLS-YAP15SA(nuclear localized),YAP1S94A(incapable of binding to the TEA domain transcription factor family)and YAP1S127D(cytoplasmic localized),and used Cell Counting Kit-8(CCK-8)assays,5-ethynyl-2’-deoxyuridine(EdU)incorporation assays,and Western blotting(WB)analysis to detect cell proliferation and apoptosis.The specific mechanism of cytoplasmic YAP1-mediated endosomal sorting complexes required for transport III(ESCRT-III)assembly was studied by co-immunoprecipitation,immunofluorescence staining,and WB analysis.Epigallocatechin gallate(EGCG)was used to simulate YAP1 retention in the cytoplasm in in vitro and in vivo experiments to study the function of cytoplasmic YAP1.YAP1 binding to NEDD4-like E3 ubiquitin protein ligase(NEDD4L)was identified using mass spectrometry and was verified in vitro.Breast tissue microarrays were used to analyze the relationship between cytoplasmic YAP1 expression and the survival of breast cancer patients.Results:YAP1 was mainly expressed in the cytoplasm in breast cancer cells.Cytoplasmic YAP1 promoted autophagic death of breast cancer cells.Cytoplasmic YAP1 bound to the ESCRT-III complex subunits charged multivesicular body protein 2B(CHMP2B)and vacuolar protein sorting 4 homolog B(VPS4B),promoting assembly of CHMP2B-VPS4B and activating autophagosome formation.EGCG retained YAP1 in the cytoplasm,promoting the assembly of CHMP2B-VPS4B to promote autophagic death of breast cancer cells.YAP1 bound to NEDD4L,and NEDD4L mediated ubiquitination and degradation of YAP1.Breast tissue microarrays revealed that high levels of cyt

关键词： Autophagosome closure Autophagy Breast cancer CHMP2B Cytoplasmic YAP1 EGCG ESCRT-III Hippo pathway NEDD4L Ubiquitin VPS4B

IFN-inducible p47 GTPases display differential responses to Schistosoma japonicum acute infection

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Cellular & Molecular Immunology 2010年第1期7卷 69-76页

作者： xuesong chen Xiaotang Du Meijuan Zhang Donghui Zhang Minjun Ji Guanling Wu Department of Pathogen Biology and Immunology Nanjing Medical UniversityNanjingChina Jiangsu Province Key Laboratory of Modern Pathogen Biology NanjingChina

Interferon gamma induced GTPase(IGTP)(also named lrgm3)and interferon gamma inducible protein 47(IRG-47)(also named lIrgd)are interferon(IFN)-inducible p47 GTPases that have been shown to regulate host resistance to intracellular pathogens.Little knowledge has been known about the role of p47 GTPases in host responses against extracellular pathogens.To investigate possible roles of IGTP and IRG-47 in the course of Schistosoma japonicum infection,IGTP and IRG-47 knockout and wild-type(WT)mice were challenged with cercariae of S.japonicum,and host responses were analyzed.At the acute stage of S.japonicum infection,mice that lacked IGTP displayed similar parasite burden and pathological damage to WT mice.Importantly,S.japonicum-infected lRG-47-deficient mice,in contrast to IGTP-deficient mice and WT mice,showed significantly reduced worms and lower egg-burden,but intense granulomatous reaction evoked by schistosome eggs in peripheral parts of liver lobes.In addition,upregulation of inflammation-related gene expression was observed in the spleen of IRG-47-deficient mice using oligonucleotide microarrays,in which multiple pathways of cytokine-cytokine receptor interaction,T-cell receptor signaling,complement,coagulation cascades and cell adhesion molecules were highlighted.Taken together,these data suggest that IGTP and IRG-47 might have distinct features that were differentially required for resistance to S.japonicum.

关键词： acute infection immunity p47 GTPase Schistosoma japonicum

Shared Gene Regulation during Human Somatic Cell Reprogramming

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Journal of Genetics and Genomics 2012年第12期39卷 613-623页

作者： Xiang Wang xuesong chen Huijun Zhang Wenyi Qin Yan Xue Fanyi Zeng Laboratory of Developmental Biology Institute of Medical ScienceSchool of MedicineShanghai Jiao Tong University Shanghai Institute of Medical Genetics Shanghai Children's HospitalShanghai Jiao Tong University

Human induced pluripotent stem （iPS） cells have the ability to differentiate into all somatic cells and to maintain unlimited self- renewal. Therefore, they have great potential in both basic research and clinical therapy for many diseases. To identify potentially universal mechanisms of human somatic cell reprogramming, we studied gene expression changes in three types of cells undergoing reprogramming. The set of 570 genes commonly regulated during induction of iPS cells includes known embryonic stem （ES） cell markers and pluripotency related genes. We also identified novel genes and biological categories which may be related to somatic cell reprogramming. For example, some of the down-regulated genes are predicted targets of the pluripotency microRNA cluster miR302/367, and the proteins from these putative target genes interact with the stem cell pluripotency factor POU5F1 according to our network analysis. Our results identified candidate gene sets to guide research on the mechanisms operating during somatic cell reprogramming.

关键词： Somatic cell reprogramming Human iPS cells Gene expression profile Network analysis

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基于“业审融合”的数智化审计实现路径探究

石油化工管理干部学院学报 2024年第2期26卷 46-49页

作者：陈雪嵩中国石化审计部北京100728

进入新时代,党和国家明确指出要“坚持科技强审,加强审计信息化建设”。本文结合中国石化数智化审计的实践经验与做法,提出了基于“业审融合”发展理念的数智化审计概念、特征,阐明了“数据+场景+平台+应用”有机结合的数智化审计实现路... 详细信息

进入新时代,党和国家明确指出要“坚持科技强审,加强审计信息化建设”。本文结合中国石化数智化审计的实践经验与做法,提出了基于“业审融合”发展理念的数智化审计概念、特征,阐明了“数据+场景+平台+应用”有机结合的数智化审计实现路径,总结了基于“业审融合”的数智化审计应用成效,对加速推进审计数智化转型具有一定参考价值和启示作用。

关键词：业审融合数智化审计实现路径应用成效

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数字化时代下企业并购的风险管理与决策策略

市场周刊 2024年第5期37卷 69-72页

作者：陈雪松宜昌茶业集团有限公司湖北宜昌443003

在数字化时代,企业并购面临信息技术风险、企业文化整合风险、人才流失风险和法律监管风险等多重风险。信息技术风险涉及数据安全漏洞、技术兼容性问题和潜在的运营中断,要求彻底审计和风险评估。文化整合风险关注员工不满和流失,需要... 详细信息

在数字化时代,企业并购面临信息技术风险、企业文化整合风险、人才流失风险和法律监管风险等多重风险。信息技术风险涉及数据安全漏洞、技术兼容性问题和潜在的运营中断,要求彻底审计和风险评估。文化整合风险关注员工不满和流失,需要深入诊断企业文化差异并设计融合方案。人才流失风险源于结构调整和管理变动,导致技能错位和员工不安全感,影响企业创新能力和市场竞争力。法律监管风险则与数据治理和反垄断法规相关,要求企业在法律框架内操作。为应对这些风险,企业需加强尽职调查,重视文化融合,完善员工激励和留任机制,以及优化并购方案,以提高监管可接受性。

关键词：数字化并购风险管理信息技术风险

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衡水湖生态环境保护与修复研究

衡水学院学报 2024年第4期26卷 1-4页

作者：陈雪松牟玉乾衡水市生态环境局河北衡水053000

衡水湖是华北平原唯一保持水域、草甸、沼泽、林地等完整湿地生态系统的自然保护区。目前衡水湖生态环境保护面临湖水循环流动性差、引水水质和水量不能得到有效保证、人类生产生活与自然保护区矛盾突出等主要问题。因此,应采取加强引... 详细信息

衡水湖是华北平原唯一保持水域、草甸、沼泽、林地等完整湿地生态系统的自然保护区。目前衡水湖生态环境保护面临湖水循环流动性差、引水水质和水量不能得到有效保证、人类生产生活与自然保护区矛盾突出等主要问题。因此,应采取加强引水保障、湖水内外源治理、开展生态修复与建设等措施,推动衡水湖湿地生态系统的保护与治理。

关键词：衡水湖生态保护修复引水水量水质改善

基于国产FPGA的可选算法引擎的密码模块实现

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北京电子科技学院学报 2024年第1期32卷 12-22页

作者：陈雪松赵海淇李秀滢北京电子科技学院北京100070

在国产FPGA芯片上设计并实现密码算法是服务于我国关键基础设施建设的一项举措。为探讨新型密码算法模块实现的可行性,基于国产FPGA芯片设计了一款可选算法引擎的硬件密码模块,该模块可集成于嵌入式系统中,基于SPI接口实现了两种分组密... 详细信息

在国产FPGA芯片上设计并实现密码算法是服务于我国关键基础设施建设的一项举措。为探讨新型密码算法模块实现的可行性,基于国产FPGA芯片设计了一款可选算法引擎的硬件密码模块,该模块可集成于嵌入式系统中,基于SPI接口实现了两种分组密码算法引擎的自主选择以及多组数据的一次性加解密处理。在此基础上,对比分析了在国产FPGA实现的SM4算法引擎和uBlock算法引擎的性能,并将SM4算法部署在国内和国外两款同档次的FPGA芯片上,对两种芯片的性能等方面进行对比分析。实验结果可知,利用国产FPGA芯片实现硬件密码模块具有较好的可行性,为FPGA的国产化替代提供一定的借鉴经验。

关键词：国产FPGA SM4 uBlock 可选算法紫光同创 Altera

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呼吸系统疾病知识图谱的构建

计算机与数字工程 2024年第1期52卷 247-250,265页

作者：陈雪松张明磊王浩畅东北石油大学电气信息工程学院大庆163318

呼吸系统疾病是中国十大死亡率疾病之一。为解决其数据分布庞杂、难以深层次应用的问题,通过采用自顶向下的方法,构建了呼吸系统疾病知识图谱。首先,参考医学文献构建了呼吸疾病词典,并根据该疾病词典爬取了垂直医疗网站的呼吸疾病数据... 详细信息

呼吸系统疾病是中国十大死亡率疾病之一。为解决其数据分布庞杂、难以深层次应用的问题,通过采用自顶向下的方法,构建了呼吸系统疾病知识图谱。首先,参考医学文献构建了呼吸疾病词典,并根据该疾病词典爬取了垂直医疗网站的呼吸疾病数据;然后,利用知识融合完成多源异构数据的链接,通过定义不同疾病属性相似度来计算疾病实体相似度,提高准确率;最后,将呼吸系统疾病数据存储到Neo4j中,并做可视化展示。

关键词：知识图谱呼吸疾病知识融合图数据库