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3D Printed Integrated Gradient-Conductive MXene/CNT/Polyimide Aerogel Frames for Electromagnetic Interference Shielding with Ultra-Low Reflection

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Nano-Micro Letters 2023年第3期15卷 332-345页

作者： Tiantian Xue Yi Yang Dingyi Yu Qamar Wali Zhenyu Wang xuesong cao Wei Fan Tianxi Liu State Key Laboratory for Modification of Chemical Fibers and Polymer Materials College of Materials Science and EngineeringDonghua University2999 North Renmin RoadShanghai 201620People’s Republic of China Key Laboratory of Synthetic and Biological Colloids Ministry of EducationSchool of Chemical and Material EngineeringJiangnan UniversityWuxi 214122People’s Republic of China Institute of Environmental Processes and Pollution Control School of Environment and Civil EngineeringJiangnan UniversityWuxi 214122People’s Republic of China NUTECH School of Applied Sciences&Humanities National University of TechnologyIslamabad 44000Pakistan

Construction of advanced electromagnetic interference(EMI)shielding materials with miniaturized,programmable structure and low reflection are promising but ***,an integrated transition-metal carbides/carbon nanotube/polyimide(gradient-conductive MXene/CNT/PI,GCMCP)aerogel frame with hierarchical porous structure and gradient-conductivity has been constructed to achieve EMI shielding with ultra-low *** gradient-conductive structures are obtained by continuous 3D printing of MXene/CNT/poly(amic acid)inks with different CNT contents,where the slightly conductive top layer serves as EM absorption layer and the highly conductive bottom layer as reflection *** addition,the hierarchical porous structure could extend the EM dissipation path and dissipate EM by multiple ***,the GCMCP aerogel frames exhibit an excellent average EMI shielding efficiency(68.2 dB)and low reflection(R=0.23).Furthermore,the GCMCP aerogel frames with miniaturized and programmable structures can be used as EMI shielding gaskets and effectively block wireless power transmission,which shows a prosperous application prospect in defense industry and aerospace.

关键词： 3D printing MXene/CNT/Polyimide aerogel Gradient-conductive Electromagnetic interference shielding

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Simple method for transformation and gene editing in medicinal plants

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Journal of Integrative Plant Biology 2024年第1期66卷 17-19页

作者： xuesong cao Hongtao Xie Minglei Song Lianghui Zhao Hailiang Liu Guofu Li Jian‐Kang Zhu Institute of Advanced Biotechnology and School of Life Sciences Southern University of Science and TechnologyShenzhen 518055China Bellagen Biotechnology Co.Ltd. Jinan 250000China Institute for Regenerative Medicine Shanghai East HospitalTongji University School of MedicineShanghai 200123China Ministry of Agriculture and Rural Affairs Key Laboratory of Gene Editing Technologies Nanfan Research InstituteChinese Academy of Agricultural SciencesSanya 572024China

Gene-editing technologies have ushered in a significant advancement in plant genetics research and molecular ***,a critical challenge hindering the widespread adoption of these technologies is the efficient delivery of gene-editing *** predominant methods for introducing these tools into plants typically involve Agrobacterium tumefaciens-mediated transformation or particle bombardment(Mao et al.,2019).Unfortunately,these traditional gene delivery methods require delicate and timeconsuming tissue culture procedures and show limited success,especially in medicinal and other less studied plants.A recent breakthrough is the development of the Cut-Dip-Budding(CDB)gene delivery *** CDB system is highly effective for plants with root-suckering *** allows the delivery of transgenes and gene-editing tools into plants through hairy root induction followed by shoot regeneration from transformed hairy roots,bypassing the need for tissue culture processes(cao et al.,2023).

关键词： transformation culture breakthrough

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Targeted G-to-T base editing for generation of novel herbicide-resistance gene alleles in rice

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Journal of Integrative Plant Biology 2024年第6期66卷 1048-1051页

作者： Yifu Tian Xinbo Li Jiyong Xie Zai Zheng Rundong Shen xuesong cao Mugui Wang Chao Dong Jian-Kang Zhu Ministry of Agriculture and Rural Affairs Key Laboratory of Gene Editing Technologies(Hainan) Institute of Crop Sciences and National Nanfan Research InstituteChinese Academy of Agricultural SciencesSanya 572024China Hainan Seed Industry Laboratory Sanya 572024China Shanghai Center for Plant Stress Biology CAS Center for Excellence in Molecular Plant SciencesThe Chinese Academy of SciencesShanghai 201602China University of Chinese Academy of Sciences Beijing 100049China Institute of Advanced Biotechnology and School of MedicineSouthern University of Science and TechnologyShenzhen 518055China

Deaminase-based cytosine base editors(CBEs)and adenine base editors(ABEs)induce C-to-T and A-to-G transitions,respectively,enabling single-nucleotide variants(SNVs)in plants for research and crop enhancement(Li et al.,2023a).The C-to-G base editors(CGBEs)and A-to-Y base editors(AYBEs),developed by incorporating glycosylases with CBEs and ABEs,expand the repertoire of base editing products,allowing C-to-T/G and A-to-T/G transversions in plants(Li et al.,2023b,2023c;Sretenovic et a.,2021).

关键词： base alleles CBE

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Breeding exceptionally fragrant soybeans for soy milk with strong aroma

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Journal of Integrative Plant Biology 2024年第4期66卷 642-644页

作者： Hongtao Xie Minglei Song xuesong cao Qingfeng Niu Jianhua Zhu Shasha Li Xin Wang Xiaomu Niu Jian-Kang Zhu Bellagen Biotechnology Co.Ltd. Jinan 250000China Institute of Advanced Biotechnology and School of Life Sciences Southern University of Science and TechnologyShenzhen 518055China School of Life Sciences Anhui Agricultural UniversityHefei 230036China Shandong Normal University Jinan 250000China

Soybean(Glycine max(L.)Merr.)is a major source of vegetable protein and oil in human diet and animal *** seeds have been extensively used in various food products and *** quality,particularly the aroma,affects cooking and eating,and ultimately influences consumer *** milk is particularly popular in China and has been gaining popularity in many other countries in the world.

关键词： exceptional soybean cooking

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High-throughput genome editing in rice with a virus-based surrogate system

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Journal of Integrative Plant Biology 2023年第3期65卷 646-655页

作者： Yifu Tian Dating Zhong Xinbo Li Rundong Shen Han Han Yuqin Dai Qi Yao Xuening Zhang Qi Deng xuesong cao Jian-Kang Zhu Yuming Lu Shanghai Center for Plant Stress Biology Center for Excellence in Molecular Plant SciencesChinese Academy of SciencesShanghai 201602China Center for Advanced Bioindustry Technologies Institute of Crop SciencesChinese Academy of Agricultural SciencesBeiing 100081China Hainan Yazhou Bay Seed Lab Sanya 572024China Shanghai Collaborative Innovation Center of Agri Seeds Joint Center for Single Cell BiologySchool of Agriculture and BiologyShanghai Jiao Tong UniversityShanghai 200240China Institute of Advanced Biotechnology School of Life SciencesSouthern University of Science and TechnologyShenzhen 518055China

With the widespread use of clustered regularly interspaced palindromic repeats(CRISPR)/CRISPR-associated nuclease(Cas) technologies in plants, large-scale genome editing is increasingly needed. Here, we developed a geminivirus-mediated surrogate system, called Wheat Dwarf Virus-Gate(WDV-surrogate), to facilitate high-throughput genome ***-Gate has two parts: one is the recipient callus from a transgenic rice line expressing Cas9 and a mutated hygromycin-resistant gene(HygM) for surrogate selection;the other is a WDV-based construct expressing two single guide RNAs(sgRNAs) targeting HygM and a gene of interest, respectively. We evaluated WDV-Gate on six rice loci by producing a total of 874 T_0 plants. Compared with the conventional method, the WDV-Gate system, which was characterized by a transient and high level of sgRNA expression, significantly increased editing frequency(66.8% vs. 90.1%), plantlet regeneration efficiency(2.31-fold increase), and numbers of homozygous-edited plants(36.3%vs. 70.7%). Large-scale editing using pooled sg RNAs targeting the SLR1 gene resulted in a high editing frequency of 94.4%, further demonstrating its feasibility. We also tested WDVGate on sequence knock-in for protein *** co-delivering a chemically modified donor DNA with the WDV-Gate plasmid, 3xFLAG peptides were successfully fused to three loci with an efficiency of up to 13%. Thus, by combining transiently expressed sgRNAs and a surrogate selection system, WDV-Gate could be useful for high-throughput gene knock-out and sequence knock-in.

关键词： genome editing high-throughput protein tagging rice

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Rapid and dynamic detection of endogenous proteins through in locus tagging in rice

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Plant Communications 2024年第11期5卷 14-22页

作者： Yifu Tian Dating Zhong Rundong Shen Xinhang Tan Chen Zhu Kai Li Qi Yao Xinbo Li Xuening Zhang xuesong cao Pengcheng Wang Jian-Kang Zhu Yuming Lu Shanghai Collaborative Innovation Center of Agri-Seeds Joint Center for Single Cell BiologySchool of Agriculture and BiologyShanghai Jiao Tong UniversityShanghai 200240China Ministry of Agriculture and Rural Affairs Key Laboratory of Gene Editing Technologies(Hainan) Institute of Crop Sciences and National Nanfan Research InstituteChinese Academy of Agricultural SciencesSanyaHainan 572024China Shanghai Center for Plant Stress Biology CAS Center for Excellence in Molecular Plant SciencesChinese Academy of SciencesShanghai 201602China Institute of Advanced Biotechnology and School of Medicine Southern University of Science and TechnologyShenzhen 518055China Hainan Seed Industry Laboratory SanyaHainan 572024China

Understanding the behavior of endogenous proteins is crucial for functional genomics, yet their dynamic characterization in plants presents substantial challenges. Whereas mammalian studies have leveraged in locus tagging with the luminescent HiBiT peptide and genome editing for rapid quantification of native proteins, this approach remains unexplored in plants. Here, we introduce the in locus HiBiT tagging of rice proteins and demonstrate its feasibility in plants. We found that although traditional HiBiT blotting works in rice, it failed to detect two of the three tagged proteins, a result attributable to low luminescence activity in plants. To overcome this limitation, we engaged in extensive optimization, culminating in a new luciferin substrate coupled with a refined reaction protocol that enhanced luminescence up to 6.9 fold. This innovation led to the development of TagBIT (tagging with HiBiT), a robust method for high-sensitivity protein characterization in plants. Our application of TagBIT to seven rice genes illustrates its versatility on endogenous proteins, enabling antibody-free protein blotting, real-time protein quantification via luminescence, in situ visualization using a cross-breeding strategy, and effective immunoprecipitation for analysis of protein interactions. The heritable nature of this system, confirmed across T1 to T3 generations, positions TagBIT as a powerful tool for protein study in plant biology.

关键词： breeding fir endogenous

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Knockout of miR396 genes increases seed size and yield in soybean

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Journal of Integrative Plant Biology 2024年第6期66卷 1148-1157页

作者： Hongtao Xie Fei Su Qingfeng Niu Leping Geng xuesong cao Minglei Song Jinsong Dong Zai Zheng Rui Guo Yang Zhang Yuanwei Deng Zhanbo Ji Kang Pang Jian‐Kang Zhu Jianhua Zhu School of Life Sciences Anhui Agricultural UniversityHefei 230036China Research Center for Biological Breeding Technology Research Institute of Frontier ScienceAnhui Agricultural UniversityHefei 230036China Bellagen Biotechnology Co.Ltd Jinan 250000China Institute of Crop Sciences/National Nanfan Research Institute Chinese Academy of Agricultural SciencesKey Laboratory of Gene Editing Technologies(Hainan)Ministry of Agriculture and Rural AffairsSanya 572025China Southern University of Science and TechnologyShenzhen 518055China Hainan Yazhou Bay Seed Laboratory Sanya 572024China

Yield improvement has long been an important task for soybean breeding in the world in order to meet the increasing demand for food and animal *** R396 genes have been shown to negatively regulate grain size in rice,but whether mi R396 family members may function in a similar manner in soybean is ***,we generated eight soybean mutants harboring different combinations of homozygous mutations in the six soybean mi R396genes through genome editing with clustered regularly interspaced palindromic repeats(CRISPR)/CRISPR-associated nuclease(Cas)12SF01 in the elite soybean cultivar Zhonghuang 302(ZH302).Four triple mutants(mir396aci,mir396acd,mir396adf,and mir396cdf),two quadruple mutants(mir396-abcd and mir396acfi),and two quintuple mutants(mir396abcdf and mir396bcdfi)were *** found that plants of all the mir396 mutants produced larger seeds compared to ZH302 *** tests showed that mir396adf and mir396cdf plants have significantly increased yield in growth zones with relatively high latitude which are suited for ZH302 and moderately increased yield in lower *** contrast,mir396abcdf and mir396bcdfiplants have increased plant height and decreased yield in growth zones with relatively high latitude due to lodging issues,but they are suited for low latitude growth zones with increased yield without lodging *** together,our study demonstrated that loss-of-function of mi R396 genes leads to significantly enlarged seed size and increased yield in soybean,providing valuable germplasms for breeding high-yield soybean.

关键词： Cas12SF01 CRISPR/Cas miR396 seed size soybean yield

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Cut-dip-budding delivery system enables genetic modifications in plants without tissue culture

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The Innovation 2023年第1期4卷 1-8页

作者： xuesong cao Hongtao Xie Minglei Song Jinghua Lu Ping Ma Boyu Huang Mugui Wang Yifu Tian Fan Chen Jun Peng Zhaobo Lang Guofu Li Jian-Kang Zhu Shanghai Center for Plant Stress Biology Center for Excellence in Molecular Plant SciencesChinese Academy of SciencesShanghai 201602China Shandong Shunfeng Biotechnology Co.Ltd. Jinan 250000China University of the Chinese Academy of Sciences Beijing 100049China Institute of Advanced Biotechnology and School of Life Sciences Southern University of Science and TechnologyShenzhen 518055China Center for Advanced Bioindustry Technologies Chinese Academy of Agricultural SciencesBeijing 100081China Hainan Yazhou Bay Seed Laboratory Sanya 572025China

Of the more than 370000 species of higher plants in nature,fewer than 0.1%can be geneticallymodified due to limitations of the current gene delivery *** for those that can be genetically modified,the modification involves a tedious and costly tissue culture ***,we describe an extremely simple cut-dip-budding(CDB)delivery system,which uses Agrobacterium rhizogene to inoculate explants,generating transformed roots that produce transformed buds due to root *** have successfully used CDB to achieve the heritable transformation of plant species inmultiple plant families,including two herbaceous plants(Taraxacum kok-saghyz and Coronilla varia),a tuberous root plant(sweet potato),and three woody plant species(Ailanthus altissima,Aralia elata,and Clerodendrum chinense).These plants have previously been difficult or impossible to transform,but the CDB method enabled efficient transformation or gene editing in them using a very simple explant dipping protocol,under non-sterile conditions and without the need for tissue *** work suggests that large numbers of plants could be amenable to genetic modifications using the CDB method.

关键词： process. enable transformed

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党的十八大以来党内监督理念与实践的新发展

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社会主义研究 2016年第4期 61-68页

作者：曹雪松中国纪检监察学院

强化党内监督是加强党的建设、巩固党的执政地位的关键举措,更是全面从严治党的重要体现。党的十八大以来,以习近平同志为总书记的党中央在加强和改进党的自身建设过程中,积极探索强化党内监督的有效途径,不断提升党内监督科学化水平,... 详细信息

强化党内监督是加强党的建设、巩固党的执政地位的关键举措,更是全面从严治党的重要体现。党的十八大以来,以习近平同志为总书记的党中央在加强和改进党的自身建设过程中,积极探索强化党内监督的有效途径,不断提升党内监督科学化水平,提出了一系列监督新理念。在党内监督路径上强调作风建设的契入与国家治理的协同;在党内监督策略上把握治标与治本的关系;在党内监督能力上采取内涵式发展提升;在党内监督指向上突出纪律与规矩;在党内监督制度建设上进一步重视实践取向;在党内监督要素上着重强化民主集中制、批评和自我批评、抓住"关键少数",有力地指导了新形势下的党内监督实践。

关键词：党内监督全面从严治党执政党建设

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XML优化技术在H.323视频会议系统中的应用

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计算机工程 2006年第9期32卷 206-208页

作者：曹雪松边学工张新晨武汉大学计算机学院武汉430079 武汉大学多媒体网络通信工程湖北省重点实验室武汉430079

为满足电信运营级视讯会议的需要,提出了一种基于分布式架构的H.323视频会议系统平台。针对这种服务平台中存在的交互信令复杂、异构信息繁多的问题,提出了利用了XML技术对该系统的指令交互模式和存储模式进行重构的优化方案,从而简化... 详细信息

为满足电信运营级视讯会议的需要,提出了一种基于分布式架构的H.323视频会议系统平台。针对这种服务平台中存在的交互信令复杂、异构信息繁多的问题,提出了利用了XML技术对该系统的指令交互模式和存储模式进行重构的优化方案,从而简化了系统的内部通信模式并提高了系统的数据管理效能,通过实例验证了这种基于XML优化后的系统在信息管理及性能扩展方面确实有一定优势。

关键词： XML H.323 视频会议

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