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Degradation properties of fulvic acid and its microbially driven mechanism from a partial nitritation bioreactor through multi-spectral and bioinformatic analysis

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Journal of Environmental Sciences 2024年第1期135卷 318-331页

作者： Quanhao Dou Li zhang Tingjun Dong Zixuan Song xuepeng Fan Yongzhen Peng Xiayan Wang Jiachun Yang National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery EngineeringBeijing University of TechnologyBeijing 100124China Department of Chemistry and Biology Center of Excellence for Environmental Safety and Biological EffectsBeijing Key Laboratory for Green Catalysis and SeparationBeijing University of TechnologyBeijing 100124China Environmental Protection Development Group Co. Ltd.Shandong 250101China

This study employed multispectral techniques to evaluate fulvic acid(FA)compositional characteristic and elucidate its biodegradation mechanisms during partial nitritation(PN)process.Results showed that FA removal efficiency(FRE)decreased from 90.22 to 23.11%when FA concentrations in the reactor were increased from 0 to 162.30 mg/L,and that molecular size,degree of aromatization and humification of the effluent FA macromolecules all increased after treatment.Microbial population analysis indicated that the proliferation of the Comamonas,OLB12 and Thauera exhibit high FA utilization capacity in lower concentrations(<50.59 mg/L),promoting the degradation and removal of macromolecular FA.In addition,the sustained increase in external FA may decrease the abundance of above functional microorganisms,resulting in a rapid drop in FRE.Furthermore,from the genetic perspective,the elevated FA levels restricted carbohydrate(ko00620,ko00010 and ko00020)and nitrogen(HAO,AMO,NIR and NOR)metabolism-related pathways,thereby impeding FA removal and total nitrogen loss associated with N_(2)O emissions.

关键词： Partial nitritation Fulvic acid 3DEEM-PARAFAC UV/Vis Microbially driven mechanism

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Effect of sodium starch octenyl succinate-based Pickering emulsion on the physicochemical properties of hairtail myofibrillar protein gel subjected to multiple freeze-thaw cycles

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Food Science and Human Wellness 2024年第2期13卷 1018-1028页

作者： Huinan Wang Jiaxin zhang Xinran Liu Jinxiang Wang xuepeng Li Jianrong Li Key Laboratory of Food Nutrition and Safety Ministry of EducationCollege of Food Science and EngineeringTianjin University of Science and TechnologyTianjin 300457China College of Food Science and Technology Bohai UniversityNational R&D Branch Center of Surimi and Surimi Products ProcessingJinzhou 121013China

A Pickering emulsion based on sodium starch octenyl succinate(SSOS)was prepared and its effects on the physicochemical properties of hairtail myofibrillar protein gels(MPGs)subjected to multiple freeze-thaw(F-T)cycles were investigated.The whiteness,water-holding capacity,storage modulus(G')and texture properties of the MPGs were significantly improved by adding 1%-2%Pickering emulsion(P<0.05).Meanwhile,Raman spectral analysis demonstrated that Pickering emulsion promoted the transformation of secondary structure,enhanced hydrogen bonds and hydrophobic interactions,and promoted the transition of disulfide bond conformation from g-g-g to g-g-t and t-g-t.At an emulsion concentration of 2%,theα-helix content decreased by 10.37%,while theβ-sheet content increased by 7.94%,compared to the control.After F-T cycles,the structure of the MPGs was destroyed,with an increase in hardness and a decrease in whiteness and water-holding capacity,however,the quality degradation of MPGs was reduced with 1%-2%Pickering emulsion.These findings demonstrated that SSOS-Pickering emulsions,as potential fat substitutes,can enhance the gel properties and the F-T stability of MPGs.

关键词： Pickering emulsion Myofibrillar protein Gel properties Freeze-thaw stability Intermolecular interactions

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Comparative metabolomics reveal histamine biodegradation mechanism by salt stressed Bacillus subtilis JZXJ-7

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Food Science and Human Wellness 2024年

作者： Rundong Wang Yijia Deng Yuhao zhang xuepeng Li Ravi Gooneratne Jianrong Li College of Food Science and Engineering Bohai University Chongqing Key Laboratory of Speciality Food Co-Built by Sichuan and Chongqing Department of Wine Food and Molecular Biosciences Faculty of Agriculture and Life Sciences Lincoln University Lincoln 7647 New Zealand College of Food Science Southwest University College of Food Science and Engineering Lingnan Normal University

Bacillus subtilis (B. subtilis) JZXJ-7 isolated from shrimp paste can significantly degrade histamine under salt stress but the mechanism is unclear. This study aims to evaluate the effect of 170 and 340 mmol/L NaCl on B. subtilis JZXJ-7 growth， histamine degradation， antioxidant enzymes [catalase (CAT)， superoxide dismutase (SOD) and glutathione S-transferase (GST)] activities and Na+， K+-ATPase activity. Furthermore， comparative metabolomics was used to investigate histamine biodegradation mechanism by B.subtilis JZXJ-7 subjected to salt stress. Both 170 and 340 mmol/L NaCl promoted B. subtilis JZXJ-7 growth in late stages of reproduction (32 - 48 h)， increased histamine degradation rate by 64.85% and 79.87% (P < 0.05)， Na+， K+-ATPase activity to 6.28 U/mg (P < 0.05) and 11.63 U/mg (P < 0.01) respectively. NaCl treatment significantly increased the activities of CAT， GST and SOD (P < 0.05)， amino acids and its metabolites (33.39%)， benzene and substituted derivatives (12.05%)， heterocyclic compounds (10.62%)， organic acids and derivatives (9.75%)， aldehydes， ketones， esters (5.59%) and nucleotides and its metabolites (4.58%). Metabolite set enrichment analysis revealed NaCl induced differential metabolic pathways of D-glutamine， D-glutamate， L-arginine， L-proline， histidine and glycerophospholipids， L-lysine degradation， and aminoacyl-tRNA biosynthesis. Exposure to 340 mmol/L NaCl up-regulated carbohydrate， glutathione and glycerophospholipid metabolism. The new insights into the mechanism of salt stress to promote B. subtilis JZJX-7 growth， energy metabolic pathways and to degrade histamine provide the theoretical basis for application of B. subtilis JZXJ-7 in food fermentation industry.

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Analysis and prediction of global vegetation dynamics:past variations and future perspectives

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Journal of Forestry Research 2023年第2期34卷 317-332页

作者： Guangchao Li Wei Chen Liqiang Mu xuepeng zhang Pengshuai Bi Zhe Wang Zhen Yang College of Geoscience and Surveying Engineering China University of Mining and TechnologyBeijing 100083People’s Republic of China School of Forestry Northeast Forestry UniversityHarbin 150040People’s Republic of China College of Information Science and Engineering Henan University of TechnologyZhengzhou 450001People’s Republic of China

Spatiotemporal dynamic vegetation changes affect global climate change,energy balances and the hydrological cycle.Predicting these dynamics over a long time series is important for the study and analysis of global environmental change.Based on leaf area index(LAI),climate,and radiation flux data of past and future scenarios,this study looked at historical dynamic changes in global vegetation LAI,and proposed a coupled multiple linear regression and improved gray model(CMLRIGM)to predict future global LAI.The results show that CMLRIGM predictions are more accurate than results predicted by the multiple linear regression(MLR)model or the improved gray model(IGM)alone.This coupled model can effectively resolve the problem posed by the underestimation of annual average of global vegetation LAI predicted by MLR and the overestimate predicted by IGM.From 1981 to 2018,the annual average of LAI in most areas covered by global vegetation(71.4%)showed an increase with a growth rate of 0.0028 a-1;of this area,significant increases occurred in 34.42%of the total area.From 2016 to 2060,the CMLRIGM model has predicted that the annual average global vegetation LAI will increase,accounting for approximately 68.5%of the global vegetation coverage,with a growth rate of 0.004 a-1.The growth rate will increase in the future scenario,and it may be related to the driving factors of the high emission scenario used in this study.This research may provide a basis for simulating spatiotemporal dynamic changes in global vegetation conditions over a long time series.

关键词： Vegetation dynamics LAI CMLRIGM Prediction Climate Radiation

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Molecular Thermal Motion Modulated Room-Temperature Phosphorescence for Multilevel Encryption

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Research 2022年第4期2022卷 597-607页

作者： Jiaqiang Zhao Guojuan Yan Wei Wang Shishi Shao Binfang Yuan Yan Jie Li xuepeng zhang Cheng Zhi Huang Peng Fei Gao Key Laboratory of Luminescence Analysis and Molecular Sensing(Southwest University) Ministry of EducationCollege of Pharmaceutical SciencesSouthwest UniversityChongqing 400715China Chongqing Key Laboratory of Inorganic Special Functional Materials Collge of Chemistry and Chemical EngineeringYangtze Normal UniversityFulingChongqing 408100China Hefei National Laboratory for Physical Science at the Microscale University of Science and Technology of China96 Jinzhai RdHefeiAnhui 230026China

The stimulus-responsive room-temperature phosphorescence(RTP)materials have become an increasingly significant topic in the fields of bioimaging,sensing,and anticounterfeiting.However,this kind of materials is scarce to date,especially for the ones with delicate stimulus-responsive behavior.Herein,a universal strategy for multilevel thermal erasure of RTP via chromatographic separation of host-guest doping RTP systems is proposed.The tunable host-guest systems,matrix materials,heating temperature,and time are demonstrated to allow precise six-level data encryption,QR code encryption,and thermochromic phosphorescence encryption.Mechanistic study reveals that the thermal-responsive property might be attributed to molecular thermal motion and the separation effect of the silica gel,which provides expanded applications of host-guest RTP materials such as cold chain break detection.This work offers a simple yet universal way to construct advanced responsive RTP materials.

关键词： RTP separation precise

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Optimized operation scheme of flash-memory-based neural network online training with ultra-high endurance

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Journal of Semiconductors 2024年第1期45卷 33-37页

作者： Yang Feng Zhaohui Sun Yueran Qi xuepeng Zhan Junyu zhang Jing Liu Masaharu Kobayashi Jixuan Wu Jiezhi Chen School of Information Science and Engineering(ISE) Shandong UniversityQingdao 266200China Neumem Co. LtdHefei 230093China Key Laboratory of Microelectronic Devices and Integrated Technology Institute of Microelectronics of Chinese Academy of SciencesBeijing 100084China Institute of Industrial Science The University of TokyoTokyoJapan

With the rapid development of machine learning,the demand for high-efficient computing becomes more and more urgent.To break the bottleneck of the traditional Von Neumann architecture,computing-in-memory(CIM)has attracted increasing attention in recent years.In this work,to provide a feasible CIM solution for the large-scale neural networks(NN)requiring continuous weight updating in online training,a flash-based computing-in-memory with high endurance(10^(9) cycles)and ultrafast programming speed is investigated.On the one hand,the proposed programming scheme of channel hot electron injection(CHEI)and hot hole injection(HHI)demonstrate high linearity,symmetric potentiation,and a depression process,which help to improve the training speed and accuracy.On the other hand,the low-damage programming scheme and memory window(MW)optimizations can suppress cell degradation effectively with improved computing accuracy.Even after 109 cycles,the leakage current(I_(off))of cells remains sub-10pA,ensuring the large-scale computing ability of memory.Further characterizations are done on read disturb to demonstrate its robust reliabilities.By processing CIFAR-10 tasks,it is evident that~90%accuracy can be achieved after 109 cycles in both ResNet50 and VGG16 NN.Our results suggest that flash-based CIM has great potential to overcome the limitations of traditional Von Neumann architectures and enable high-performance NN online training,which pave the way for further development of artificial intelligence(AI)accelerators.

关键词： NOR flash memory computing-in-memory endurance neural network online training

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Mountain tunnel under earthquake force:A review of possible causes of damages and restoration methods

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Journal of Rock Mechanics and Geotechnical Engineering 2020年第2期12卷 414-426页

作者： xuepeng zhang Yujing Jiang Kazuhiko Maegawa State Key Laboratory of Mining Disaster Prevention and Control Co-founded by Shandong Province and the Ministry of Science and Technology Shandong University of Science and TechnologyQingdao266590China School of Engineering Nagasaki UniversityNagasaki8528521Japan Nippon Expressway Research Institute Company Limited Tokyo194-8508Japan

Accurate seismic assessment and proper aseismic design of underground structures require a comprehensive understanding of seismic performance and response of underground structures under earthquake force.In order to understand the seismic behavior of tunnels during an earthquake,a wide collection of case histories has been reviewed from the available literature with respect to damage classification,to discuss the possible causes of damage,such as earthquake parameters,structural form and geological conditions.In addition,a case of Tawarayama tunnel subjected to the 2016 Kumamoto earthquake is studied.Discussion on the possible influence factors aims at improving the performancebased aseismic design of tunnels.Finally,restoration design criterion and methods are presented taking Tawarayama tunnel as an example.

关键词： Mountain tunnel Seismic damage Influence factor Restoration method

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髌上入路和髌旁内侧入路髓内钉固定治疗胫骨多段骨折的疗效比较

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中国修复重建外科杂志 2024年第6期38卷 728-733页

作者：徐学鹏赵亮范玮张喜海西南医科大学附属医院骨与关节外科四川泸州646000

目的比较膝关节半伸直位髌上入路与髌旁内侧入路髓内钉固定治疗胫骨多段骨折的临床疗效。方法回顾分析2018年7月—2022年12月收治且符合选择标准的43例采用髓内钉固定治疗的胫骨多段骨折患者临床资料,其中23例采用膝关节半伸直位髌上入... 详细信息

目的比较膝关节半伸直位髌上入路与髌旁内侧入路髓内钉固定治疗胫骨多段骨折的临床疗效。方法回顾分析2018年7月—2022年12月收治且符合选择标准的43例采用髓内钉固定治疗的胫骨多段骨折患者临床资料,其中23例采用膝关节半伸直位髌上入路(A组),20例采用膝关节半伸直位髌旁内侧入路(B组)。两组患者性别、年龄、致伤原因、受伤至手术时间、骨折国际内固定研究协会/美国骨创伤协会(AO/OTA)分型及术前疼痛视觉模拟评分(VAS)、膝关节活动度(range of motion,ROM)等基线资料比较差异均无统计学意义(P>0.05)。记录并比较两组患者手术时间、术中失血量、切口长度、术中透视次数及骨折愈合时间,统计两组术后4、8、12周膝关节VAS评分及ROM,观察术后并发症发生情况;末次随访时采用Lysholm评分评价膝关节功能。结果两组患者均顺利完成手术,术中无神经、血管损伤等并发症发生,术后所有切口均Ⅰ期愈合。两组手术时间、术中失血量、术中透视次数比较差异均无统计学意义(P>0.05);但B组手术切口长于A组,差异有统计学意义(P0.05)。两组骨折均愈合,愈合时间比较差异无统计学意义(P>0.05)。随访期间无内固定物松动、断裂及骨折复位丢失等并发症发生。末次随访时,B组膝关节Lysholm评分显著优于A组,差异有统计学意义(P<0.05)。结论膝关节半伸直位髌上入路与髌旁内侧入路髓内钉固定治疗胫骨多段骨折均可获得满意疗效,髌旁内侧入路术后早期膝关节疼痛症状更轻,远期膝关节功能更优。

关键词：胫骨多段骨折髌上入路髌旁内侧入路髓内钉

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基于参数估计-滑模阻抗控制的SEA性能分析

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重庆大学学报 2024年第5期47卷 1-12页

作者：曹学鹏张正李彩红鲁航长安大学工程机械学院机电液一体化研究所西安710064

重载四足机器人的足部与地面接触过程和步态转换过程中会受到不确定的冲击载荷作用,易导致足部机构载荷过大从而造成结构的冲击损坏。因此,针对使用液压串联弹性执行器(series elastic actuators,SEA)作为足部末端在非结构环境下动态性... 详细信息

重载四足机器人的足部与地面接触过程和步态转换过程中会受到不确定的冲击载荷作用,易导致足部机构载荷过大从而造成结构的冲击损坏。因此,针对使用液压串联弹性执行器(series elastic actuators,SEA)作为足部末端在非结构环境下动态性能差的问题,提出了基于环境参数估计的滑模阻抗控制方法(environmental parameter estimation sliding mode,EPESM)。以阀控液压缸的活塞位移传递函数为基础建立了基于位置内环的SEA阻抗控制模型,并以PID作为基础控制器;为改善SEA阻抗控制的动态性能,根据Lyapunov第二法构建稳定的自适应环境参数估计方法对SEA期望位置进行前馈补偿;为提升自适应环境参数估计方法在SEA工作过程中不同阶段的动态性能和环境变化适应性,使用模糊控制方法对自适应环境参数估计方法中的自适应参数进行寻优;以SEA状态方程为基础构建滑模控制器与PID控制器进行动态性能对比分析。仿真结果表明:在变SEA弹簧刚度工况和变环境刚度下,EPESM阻抗控制的响应速度明显更快,可将调节时间从平均5 s缩短到1 s内,能更快地达到预期位移和预期接触力,且能略微降低稳态误差,使接触力误差保持在±6 N内。在动态跟踪工况下,EPESM阻抗控制的动态性能更好,在快速进入跟踪状态后,可以长时间保持0.2 s以内的相位滞后和5.2%的幅值误差。

关键词：重载机器人串联弹性执行器阻抗控制模糊-自适应参数估计滑模控制

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超声辅助热处理联合海藻糖和谷氨酰胺转氨酶对未漂洗鱼糜凝胶特性的影响

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食品科学 2024年第7期45卷 233-242页

作者：张畅李强王嵬仪淑敏李学鹏励建荣渤海大学食品科学与工程学院生鲜农产品贮藏加工及安全控制技术国家地方联合工程研究中心国家鱼糜及鱼糜制品加工技术研发分中心辽宁锦州121013

研究海藻糖、谷氨酰胺转氨酶(glutamine transaminase,TGase)与超声波技术联用对热致未漂洗鱼糜凝胶品质的影响。首先对凝胶特性、持水性等凝胶特性指标进行测定,再对其构象及作用的变化进行分析。结果表明:在超声辅助处理的同时,添加... 详细信息

研究海藻糖、谷氨酰胺转氨酶(glutamine transaminase,TGase)与超声波技术联用对热致未漂洗鱼糜凝胶品质的影响。首先对凝胶特性、持水性等凝胶特性指标进行测定,再对其构象及作用的变化进行分析。结果表明:在超声辅助处理的同时,添加质量分数4.5%海藻糖和/或质量分数0.05%TGase使鱼糜凝胶强度提升35.11%~169.25%,其中海藻糖抑制了蛋白质结构的展开,而TGase则表现出相反的作用;但实际上超声辅助处理抑制了TGase的催化作用,使凝胶强度降低至4737.23 g·mm,游离水相对含量降低19.52%,抑制了蛋白质过度交联;此外,超声处理促进了海藻糖保护作用下的蛋白质展开,当海藻糖与TGase同时存在时,各自对蛋白质结构的影响均被削弱。

关键词：鱼糜凝胶肌球蛋白超声海藻糖谷氨酰胺转氨酶

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