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突发公共卫生事件中公众的风险感知与情绪传播研究

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中国传媒大学学报（自然科学版） 2024年第1期31卷 42-48页

作者：贺一陈雪宁金鑫重庆师范大学新闻与传媒学院重庆401331 重庆师范大学计算智能与未来传播研究所重庆401331

在风险社会背景下,社交媒体平台在应对突发性公共卫生事件,如风险感知的变迁和问题反馈解决等方面,扮演着关键角色。本研究对微博平台“新十条”发布后两个月内所涵盖的约30万条帖子进行了数据挖掘与实证分析,深入探讨社交媒体平台上风... 详细信息

在风险社会背景下,社交媒体平台在应对突发性公共卫生事件,如风险感知的变迁和问题反馈解决等方面,扮演着关键角色。本研究对微博平台“新十条”发布后两个月内所涵盖的约30万条帖子进行了数据挖掘与实证分析,深入探讨社交媒体平台上风险感知变化的规律。研究结果揭示,不同情绪对风险感知的影响存在显著差异,负面情绪“恶”的传播对风险感知的影响最为突出。

关键词：突发公共卫生事件风险感知情绪传播

A novel hierarchical targeting and controllable smart nanoparticles for enhanced in situ nuclear photodynamic therapy

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Nano Research 2022年第5期15卷 4212-4223页

作者： Gankun Yuan Qilu Wang Zifan You xuening chen Jinping Xue Xiao Jia Juanjuan chen National&Local Joint Biomedical Engineering Research Center on Photodynamic Technologies State Key Laboratory of Photocatalysis on Energy and EnvironmentCollege of ChemistryFuzhou UniversityFuzhou 350116China

Photodynamic therapy(PDT)is a promising and non-invasive treatment for various *** nuclear PDT has considerable therapeutic prospects,it is still hindered by the non-specific recognition of tumor tissues or the degradation of nuclear targeting cationic groups by enzymes in the ***,a hierarchical targeted and controlled release strategy is proposed by using folate-modified poly-β-cyclodextrin(poly-β-CD)as a nano-carrier for loading nuclear localization signals(NLSs)-conjugated photosensitizer PAP(PAP=pyropheophorbide a-PAAKRVKLD).Excitingly,the obtained FA-CD@PAP(FA=folic acid)and nanoparticles(NPs)can specifically recognize tumor cells overexpressing folate receptors(FR)to remarkedly enhance the intracellular ***,the encapsulated PAP can be released under acidic conditions to realize precise nuclear *** reactive oxygen species(ROS)generated by the intranuclear-accumulated PAP upon irradiation can oxidize and destroy DNA chains or DNA repair enzymes instantaneously,which can directly induce cell *** a result,FA-CD@PAP NPs exhibit excellent tumor regression and negligible side *** work provides an intelligent nuclear-targeted delivery strategy for in situ nuclear PDT with extremely prominent efficacy and high biological safety.

关键词： photodynamic therapy hierarchical targeted nuclear localization signal poly-β-cyclodextrin nuclear-targeted

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激光清洗钢件表面油污的工艺研究

激光技术 2024年第4期48卷 565-571页

作者：田川刘洪波潘家莹杨鸿汇陈雪宁白晓磊内蒙古大学物理科学与技术学院呼和浩特010021

为了探究激光工艺对钢件表面油污层清洗质量的影响规律,采用脉冲激光器对Q235钢板试样进行了激光除污清洗试验,并检测了试样表面清洗质量和损伤情况。理论分析了清洗过程中激光束对油污层和基体钢件作用的机理与效果,建立了激光热效应模... 详细信息

为了探究激光工艺对钢件表面油污层清洗质量的影响规律,采用脉冲激光器对Q235钢板试样进行了激光除污清洗试验,并检测了试样表面清洗质量和损伤情况。理论分析了清洗过程中激光束对油污层和基体钢件作用的机理与效果,建立了激光热效应模型,通过仿真计算与实验,分析出离焦程度对基体表面温度的影响程度;实验验证了激光清洗工艺中不同离焦程度下对Q235钢件表面油污的去除效果和钢件表面的损伤效果。结果表明,激光清洗Q235钢件表面油污层的最优工艺参数是离焦程度为5 mm,平均功率为4 W,可见离焦程度因素对清洗效果的影响远大于激光功率因素。该研究为金属表面油污处理工艺和激光清洗提供了一定参考。

关键词：激光技术激光清洗除油污 Q235钢无损清洗激光参量

Whisker of biphasic calcium phosphate ceramics: Osteoimmunomodulatory behaviors

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Nano Research 2022年第10期15卷 9169-9182页

作者： Jinjie Wu Cong Feng Menglu Wang Hongfeng Wu Xiangdong Zhu Xiangfeng Li xuening chen Xingdong Zhang National Engineering Research Center for Biomaterials College of Biomedical EngineeringSichuan UniversityChengdu 610064China

Immune systems play a critical role in the regulation of bone formation and homeostasis,which arouses a growing interest in the development of biomaterials that can modulate both immune response and *** this study,biphasic calcium phosphate(BCP)ceramics were modified with different whiskered surface,and their effects on macrophage polarization and functional status were *** results showed that compared to BCP-W ceramics with long and solid whiskers,BCPHW ceramics with short and hollow whiskers surface were conducive to protein adsorption and macrophage ***,BCP-HW ceramics down-regulated the expression of M1 macrophage markers(Il1β,Tnfα,and iNos),promoted the expression of M2 macrophage markers(Il10 and Arg)and growth factors(Tgfβ1 and Bmp2),which might be attributed to the differential integrin expression regulated by different whisker *** conditioned medium derived from the supernatant of macrophage/whiskered ceramic co-culture was further used to culture MC3T3-E1 pre-osteoblasts to evaluate the effects of whiskered ceramic-mediated macrophage secretion on osteogenesis in *** with BCP-W ones,the secretion pattern induced by BCP-HW ceramics could promote the expression of bone markers in pre-osteoblasts,which might due to the activation of intracellular signaling cascades like BMP/Smad and TGF-β/Smad signaling pathways.A murine intramuscular implantation model suggested that after implantation for 1,2,and 3 weeks,BCP-HW ceramics drove the switch of macrophages to ARG+wound-healing M2 phenotype,while BCP-W ceramics increased the proportion of iNOS+M1 inflammatory *** 2 months,only BCP-HW could induce ectopic bone *** together,these results indicated that BCP ceramics with hollow whiskers were capable of creating a proper inflammatory microenvironment to induce bone *** whiskered BCP ceramics with good osteo-immunomodulatory capacity hold promise in serving as bone gra

关键词： whisker macrophage polariztion osteoinduction biphasic calcium phosphate surface structure

Genome-wide identification of the MATE gene family and functional characterization of PbrMATE9 related to anthocyanin in pear

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Horticultural Plant Journal 2023年第6期9卷 1079-1094页

作者： Jiawen Sheng xuening chen Bobo Song Hainan Liu Jiaming Li Runze Wang Jun Wu College of Horticulture State Key Laboratory of Crop Genetics and Germplasm EnhancementNanjing Agricultural UniversityNanjingJiangsu210095China College of Horticulture and Plant Conservation Henan University of Science and TechnologyLuoyangHenan471023China

Plant multidrug and toxic compound extrusion(MATE) genes play an important role in the process of detoxification, plant morphogenesis, and anthocyanin accumulation. However, whether the MATE gene family functions in pear peel coloration is still unknown. To evaluate and identify the MATE gene family members which are involving in anthocyanin accumulation and coloration in pear. In this study, 85 MATE genes were identified in the reference pear genome of ‘Dangshansuli’ through genome-wide identification. Based on gene structure and phylogenetic tree analysis, the MATE family was divided into five subfamilies. RNA sequencing and quantitative real-time polymerase chain reaction(qRTPCR) indicated that the expression patterns of PbrMATEs were tissue-specific. 28.24%(24) of PbrMATE genes were expressed in the fruits, and44.71%(38) of PbrMATE genes were expressed in the leaves. Additionally, we found that the expression levels of PbrMATE9, PbrMATE26,PbrMATE50, and PbrMATE69 in debagged fruits with red peel were significantly higher than those in bagged fruits without red peel, according to our bagging/debagging treatment of ‘Mantianhong’. The expression pattern of PbrMATE9 was consistent with the variation trend in anthocyanin content, suggesting that it might play an important role in anthocyanin accumulation in response to light exposure. Subcellular localization showed that PbrMATE9 was a membrane protein. More strikingly, the transient overexpression of PbrMATE9 promoted anthocyanin accumulation in the peel of pear, and the expression of structural genes(PbrCHI, PbrANS, PbrDFR, and PbrUFGT) in the anthocyanin biosynthesis pathway also increased significantly. Through co-expression network analysis, the transcription factors were identified, such as WRKY, COL,GATA, and BBX, which might be involved in the regulation of PbrMATE9. The study has enriched the genetic resources and improved the understanding of the regulation network of anthocyanin accumulation in pear.

关键词： Pear Anthocyanin Coloration MATE gene family PbrMATE9 RNA-seq

The directional migration and differentiation of mesenchymal stem cells toward vascular endothelial cells stimulated by biphasic calcium phosphate ceramic

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Regenerative Biomaterials 2018年第3期5卷 129-139页

作者： Ying chen Jing Wang Xiangdong Zhu xuening chen Xiao Yang Kai Zhang Yujiang Fan Xingdong Zhang National Engineering Research Center for Biomaterials Sichuan UniversityChengdu 610064China

Osteoinductivity of porous calcium phosphate(CaP)ceramics has been widely investigated and confirmed,and it might be attributed to the rapid formation of the vascular networks after in vivo implantation of the *** this study,to explore the vascularization mechanism within the CaP ceramics,the migration and differentiation of bone marrow-derived mesenchymal stem cells(BMSCs)under the stimulation of porous biphasic calcium phosphate(BCP)ceramic with excellent osteoinductivity were systematically *** results indicated that the directional migration of BMSCs toward BCP ceramic occurred when evaluated by using a transwell model,and the BMSCs migration was enhanced by the seeded macrophages on the ceramic in ***,by directly culturing BMSCs on BCP ceramic discs under both in vitro and in vivo physiological environment,it was found that the differentiation of BMSCs toward vascular endothelial cells(VECs)happened under the stimulation of BCP ceramic,as was confirmed by the up-regulated gene expressions and protein secretions of VECs-related characteristic factors,including kinase insert domain receptor,von willebrand factor,vascular cell adhesion molecule-1 and cadherin 5 in the *** study offered a possibility for explaining the origin of VECs during the rapid vascularization process after in vivo implantation of porous CaP ceramics and could give some useful guidance to reveal the vascularization mechanism of the ceramics.

关键词： BCP ceramic vascularization BMSCs VECs migration differentiation

Hydroxyapatite nanoparticles drive the potency of Toll-like receptor 9 agonist for amplified innate and adaptive immune response

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Nano Research 2022年第10期15卷 9286-9297页

作者： Qin Zeng Ruiqi Wang Yuchen Hua Hongfeng Wu xuening chen You-cai Xiao Qiang Ao Xiangdong Zhu Xingdong Zhang National Engineering Research Center for Biomaterials Sichuan UniversityChengdu 610064China NMPA Key Laboratory for Quality Research and Control of Tissue Regenerative Biomaterials&Institute of Regulatory Science for Medical Devices&NMPA Research Base of Regulatory Science for Medical Devices Sichuan UniversityChengdu 610064China College of Biomedical Engineering Sichuan UniversityChengdu 610064China Key Laboratory of Drug-Targeting and Drug Delivery System of the Education Ministry and Sichuan Province Sichuan UniversityChengdu 610041China

The potency of Toll-like receptor 9(TLR9)agonist to drive innate immune response was limited due to immune suppression or tolerance during TLR9 signaling activation in immune *** we addressed this problem by introducing hydroxyapatite nanoparticles(HANPs)to CpG ODN(CpG),a TLR9 *** study revealed that HANPs concentration and durationdependently reprogramed the immune response by enhancing the secretion of immunostimulatory cytokines(tumor necrosis factorα(TNFα)or IL-6)while reducing the production of immunosuppressive cytokine(IL-10)in macrophages in response to ***,the enhanced immune response benefited from increased intracellular Ca2+in macrophage by the addition of ***,we found exposure to HANPs impacted the mitochondrial function of macrophages in support of the synthesis of adenosine triphosphate(ATP),the production of nicotinamide adenine dinucleotide(NAD),and reactive oxygen species(ROS)in the presence or absence of *** vaccinated mice model,only one vaccination with a mixture of CpG,HANPs,and OVA,a model antigen,allowed the development of a long-lasting balanced humoral immunity in mice without any histopathological change in the local injection ***,this study revealed that HANPs could modulate the intracellular calcium level,mitochondrial function,and immune response in immune cells,and suggested a potential combination adjuvant of HANPs and TLR9 agonist for vaccine development.

关键词： hydroxyapatite nanoparticles Toll-like receptor 9 intracellular calcium mitochondrial function adaptive immune response

Machine learning on properties of multiscale multisource hydroxyapatite nanoparticles datasets with different morphologies and sizes

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npj Computational Materials 2021年第1期7卷 1276-1286页

作者： Ziteng Liu Yinghuan Shi Hongwei chen Tiexin Qin Xuejie Zhou Jun Huo Hao Dong Xiao Yang Xiangdong Zhu xuening chen Li Zhang Mingli Yang Yang Gao Jing Ma Key Laboratory of Mesoscopic Chemistry of Ministry of Education School of Chemistry and Chemical EngineeringNanjing UniversityNanjingP.R.China State Key Laboratory for Novel Software Technology Nanjing UniversityNanjingP.R.China Kuang Yaming Honors School&Institute for Brain Sciences Nanjing UniversityNanjingP.R.China National Engineering Research Center for Biomaterials Sichuan UniversityChengduP.R.China Institute of Atomic and Molecular Physics Sichuan UniversityChengduP.R.China College of Biomedical Engineering Sichuan UniversityChengduP.R.China Jiangsu Key Laboratory of Advanced Organic Materials School of Chemistry and Chemical EngineeringNanjing UniversityNanjingP.R.China

Machine learning models for exploring structure-property relation for hydroxyapatite nanoparticles(HANPs)are still lacking.A multiscale multisource dataset is presented,including both experimental data(TEM/SEM,XRD/crystallinity,ROS,anti-tumor effects,and zeta potential)and computation results(containing 41,976 data samples with up to 9768 atoms)of nanoparticles with different sizes and morphologies at density functional theory(DFT),semi-empirical DFTB,and force field,*** geometric descriptors are set for the explainable machine learning methods to predict surface energies and surface stress of HANPs with satisfactory *** avoid the pre-determination of features,we also developed a predictive deep learning model within the framework of graph convolution neural network with good *** with DFT accuracy are achievable for largesized nanoparticles from the learned correlations and scale functions for mapping different theoretical levels and particle *** simulated XRD spectra and crystallinity values are in good agreement with experiments.

关键词： sizes sized property

Optimal regenerative repair of large segmental bone defect in a goat model with osteoinductive calcium phosphate bioceramic implants

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Bioactive Materials 2022年第5期7卷 240-253页

作者： Wei Zhi Xiaohua Wang Dong Sun Taijun chen Bo Yuan Xiangfeng Li xuening chen Jianxin Wang Zhao Xie Xiangdong Zhu Kai Zhang Xingdong Zhang Key Laboratory of Advanced Technologies of Materials Ministry of EducationSouthwest Jiaotong UniversityChengdu610031China Department of Orthopaedics First Affiliated HospitalThird Military Medical University(Army Medical University)Gaotanyan No.30400038ChongqingChina National Engineering Research Center for Biomaterials College of Biomedical EngineeringSichuan UniversityChengdu610064China

So far,how to achieve the optimal regenerative repair of large load-bearing bone defects using artificial bone grafts is a huge challenge in *** this study,a strategy of combining osteoinductive biphasic calcium phosphate(BCP)bioceramic scaffolds with intramedullary nail fixation for creating stable osteogenic microenvironment was applied to repair large segmental bone defects(3.0 cm in length)in goat femur *** material characterization results showed that the BCP scaffold had the initial compressive strength of over 2.0 MPa,and total porosity of 84%.The cell culture experiments demonstrated that the scaffold had the excellent ability to promote the proliferation and osteogenic differentiation of rat bone marrow-derived mesenchymal stem cells(BMSCs).The in vivo results showed that the intramedullary nail fixation maintained the initial stability and structural integrity of the implants at early stage,promoting the osteogenic process both guided and induced by the BCP *** 9 months postoperatively,good integration between the implants and host bone was observed,and a large amount of newborn bones formed,accompanying with the degradation of the *** 18 months postoperatively,almost the complete new bone substitution in the defect area was *** maximum bending strength of the repaired bone defects reached to the 100% of normal femur at 18 months *** results demonstrated the good potential of osteoinductive BCP bioceramics in the regenerative repair of large load-bearing bone *** current study could provide an effective method to treat the clinical large segmental bone defects.

关键词： Calcium phosphate bioceramics Large segmental bone defects Bone regenerative repair Osteoinduction Mechanical stability