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molecular Plant 2024年第2期17卷 342-358页

作者： Jianwu Li Shuaibing Yao Sang-Chul Kim xuemin wang Department of biology University of Missouri-St.LouisSt.LouisMO 63121USA Donald Danforth Plant Science Center St.LouisMO 63132USA

Lipid phosphorylation by diacylglycerol kinase(DGK)that produces phosphatidic acid(PA)plays important roles in various biological processes,including st.ess responses,but the underlying mechanisms remain ***,we show that DGK5 and its lipid product PA suppress ABA biosynthesis by interacting withABA-DEFICIENT2(ABA2),a key ABA biosynthesis enzyme,to negatively modulate plant responseto abiotic st.ess test.d in Arabidopsis *** of DGK5 function rendered plants less damaged,whereas overexpression(OE)of DGK5 enhanced plant damage to water and salt *** dgk5 mutant plants exhibited decreased total cellular and nuclear levels of PA with increased levels of diacylglycerol,whereas DGK5-OE plants displayed the opposite ***,we found that both DGK5 and PA bind to the ABA-synthesizing enzyme ABA2 and suppress its enzymatic ***,the dgk5 mutant plants exhibited increased levels of ABA,while DGK5-OE plants showed reduced ABA *** addition,we showed that both DGK5 and ABA2 are detected in and outside the nuclei,and loss of DGK5 function decreased the nuclear association of *** found that both DGK5 activity and PA promote nuclear association of *** together,these results indicate that both DGK5 and PA interact with ABA2 to inhibit its enzymatic activity and promote its nuclear sequest.ation,thereby sup-pressing ABA production in response to abiotic *** st.dy reveals a sophist.cated mechanism by which DGK5 and PA regulate plant st.ess responses.

关键词： diacylglycerol kinase phosphatidic acid diacylglycerol lipid signaling st.ess responses lipid-protein binding protein-protein interaction Arabidopsis

Dual Functions of Phospholipase Dα1 in Plant Response to Drought

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molecular Plant 2008年第2期1卷 262-269页

作者： Yueyun Hong Suqin Zheng xuemin wang Department of biology University of Missouri St Louis MO 63121 USADonald Danforth Plant Science Center St Louis MO 63132 USA

Phospholipase Dα1 （PLDα1） has been shown to mediate the abscisic acid regulation of st.matal movements. Arabidopsis plants deficient in PLDα1 increased, whereas PLDα1-overexpressing tobacco decreased, transpirational water loss. In the early st.ge of drought, the decrease in water loss was associated with a rapid st.matal closure caused by a high level of PLD in PLDα1-overexpressing plants. However, in the late st.ge of drought, the overexpressing plants displayed more susceptibility to drought than control plants. PLDα1 activity in the overexpressing plants was much higher than that of control plants in which drought also induced an increase in PLDα1 activity. The high level of PLDα1 activity was correlated to membrane degradation in late st.ges of drought, as demonst.ated by ionic leakage and lipid peroxidation. These findings indicate that a high level of PLDα1 expression has different effects on plant response to water deficits. It promotes st.matal closure at earlier st.ges, but disrupts membranes in prolonged drought st.ess. These findings are discussed in relation to the underst.nding of PLD functions and potential applications.

关键词： drought phospholipase phosphatidic acid lipid oxidation lipid signaling.

Non-specific phospholipase C4 hydrolyzes phosphosphingolipids and phosphoglycerolipids and promotes rapeseed growth and yield

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Journal of Integrative Plant biology 2023年第11期65卷 2421-2436页

作者： Bao Yang Jianwu Li Jiayu Yan Ke Zhang Zhewen Ouyang Yefei Lu Huili Wei Qing Li Xuan Yao Shaoping Lu Yueyun Hong xuemin wang Liang Guo National Key Laboratory of Crop Genetic Improvement Hubei Hongshan LaboratoryHuazhong Agricultural UniversityWuhan 430070China Department of biology University of MissouriSt.LouisMO 63121USA Donald Danforth Plant Science Center St.LouisMO 63132USA Yazhouwan National Laboratory Sanya 572025China

Phosphorus is a major nutrient vital for plant growth and development,with a subst.ntial amount of cellular phosphorus being used for the biosynthesis of membrane ***,we report that NON-SPECIFIC PHOSPHOLIPASE C4(NPC4)in rapeseed(Brassica napus)releases phosphate from phospholipids to promote growth and seed yield,as plants with altered NPC4 levels showed significant changes in seed production under different phosphate *** regularly interspaced short palindromic repeat(CRISPR)/CRISPR-associated nuclease 9(Cas9)-mediated knockout of Bna NPC4 led to elevated accumulation of phospholipids and decreased growth,whereas overexpression(OE)of Bna NPC4resulted in lower phospholipid contents and increased plant growth and seed *** demonst.ate that Bna NPC4 hydrolyzes phosphosphingolipids and phosphoglycerolipids in vitro,and plants with altered Bna NPC4 function displayed changes in their sphingolipid and glycerolipid contents in roots,with a greater change in glycerolipids than sphingolipids in leaves,particularly under phosphate deficiency *** addition,Bna NPC4-OE plants led to the upregulation of genes involved in lipid metabolism,phosphate release,and phosphate transport and an increase in free inorganic phosphate in *** results indicate that Bna NPC4 hydrolyzes phosphosphingolipids and phosphoglycerolipids in rapeseed to enhance phosphate release from membrane phospholipids and promote growth and seed production.

关键词： inorganic phosphorus non-specific phospholipase C4 phosphate deficiency phospholipids phosphorus use efficiency rapeseed

Emerging Roles of Sphingolipid Signaling in Plant Response to Biotic and Abiotic st.esses

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molecular Plant 2018年第11期11卷 1328-1343页

作者： Usman Ali Hehuan Li xuemin wang Liang Guo National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural UniversityWuhan 430070China Department of biology University of MissouriSt.LouisMO 63121USA Donald Danforth Plant Science Center St.LouisMO 63132USA

Plant sphingolipids are not only st.uctural components of the plasma membrane and other endomembrane syst.ms but also act as signaling molecules during biotic and abiotic ***,the roles of sphingolipids in plant signal transduction in response to environmental cues are yet to be invest.gated in detail. In this review,we discuss the signaling roles of sphingolipid metabolites with a focus on plant *** also mention some microbial sphingolipids that initiate signals during their interaction with plants, because of the limited literatures on their plant *** equilibrium of nonphosphorylated and phosphorylated sphingolipid species determine the dest.ny of plant cells,whereas molecular connections among the enzymes responsible for this equilibrium in a coordinated signaling network are poorly underst.od.A mechanist.c link between the phytohormone-sphingolipid interplay has also not yet been fully underst.od and many key participants involved in this complex interaction operating under st.ess conditions await to be *** research is needed to fill these gaps and to better underst.nd the signal pathways of plant sphingolipids and their interplay with other signals in response to environmental st.esses.

关键词： sphingolipids long-chain bases sphingolipid signaling biotic st.ess abiotic st.ess

Phospholipase D and phosphatidic acid signalling in plant response to drought and salinity

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Phospholipase D and phosphatidic acid signalling in plant re...

Transcriptional Regulation of Lipid Catabolism during Seedling Est.blishment

第六届中国植物逆境生理学与分子生物学学术研讨会

作者： Yueyun Hong1,Wenhua Zhang2,xuemin wang3 1National Key Laboratory of Crop Genetic Improvement,Huazhong Agricultural university,Wuhan 430070,China 2College of Life Sciences,st.te Key Laboratory of Crop Genetics and Germplasm Enhancement,Nanjing Agricultural university,Nanjing 210095,China,and 3Department of biology,university of missouri,st.louis,mo 63121,USA Donald Danforth Plant Science Center,st.louis,mo 63132,USA

＜正＞The activity of phospholipase D（PLD）in plants increases under different hyperosmotic st.esses,such as dehydration,drought,and *** results begin to shed light onto the involvement of PLD in response to water deficits and *** PLDs have unique and overlapping functions in these ***α1promotes st.matal closure and reduces water ***α1and PLDδare involved in seedling tolerance to salt st.ess.

关键词： Plants hyperosmotic st.ess phospholipid signalling

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molecular Plant 2020年第7期13卷 984-1000页

作者： Guangqin Cai Sang-Chul Kim Jianwu Li Yongming Zhou xuemin wang National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural UniversityWuhan 430070China Department of biology University of MissouriSt.LouisMO 63121USA Donald Danforth Plant Science Center St.LouisMO 63132USA Henan Agricultural university ZhengzhouHenan 450002China

Lipid catabolism in germinating seeds provides energy and subst.ates for initial seedling growth,but how this process is regulated is not well ***,we show that an AT-hook motif-containing nuclear localized(AHL)protein regulates lipid mobilization and fatty acid p-oxidation during seed germination and seedling ***4 was identified to directly interact with the lipid mediator phosphatidic acid(PA).Knockout(KO)of AHL4 enhanced,but overexpression(OE)of AHL4 attenuated,triacylglycerol(TAG)degradation and seedling *** seedling growth of the OE lines was rest.red by sucrose supplementation to the growth ***4-OE seedlings displayed decreased expression of genes involved in TAG hydrolysis and fatty acid oxidation,whereas the opposite was observed in *** genes contained AHL4-binding cis elements,and AHL4 was shown to bind to the promoter regions of genes encoding the TAG lipases SDP1 and DALL5 and acyl-thioest.rase *** AHL4-DNA interactions were suppressed by PA species that bound to *** results indicate that AHL4 suppresses lipid catabolism by repressing the expression of specific genes involved in TAG hydrolysis and fatty acid oxidation,and that PA relieves AHL4-mediated suppression and promotes TAG ***,AHL4 and PA together regulate lipid degradation during seed germination and seedling est.blishment.

关键词： lipid catabolism lipid regulation phosphatidic acid seed germination seedling est.blishment transcriptional regulation

Patterns and Timing in Expression of Early Auxin-Induced Genes Imply Involvement of Phospholipases A （pPLAs） in the Regulation of Auxin Responses

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molecular Plant 2013年第5期6卷 1473-1486页

作者： Corinna Labusch Maria Shishova Yunus Effendi Maoyin Li xuemin wang Gunther RE. Scherera Leibniz Universit&t Hannover Institut for Zierpflanzenbau und Geh61zwissenschaften Abt. Molekulare Ertragsphysiologie Herrenhauser Str. 2 D-30419 Hannover Germany Department of Plant Physiology and Biochemist.y State University of St. Petersburg Universitetskaya em. 7/9 St. Petersburg 199034 Russia Department of biology University of Missouri St Louis MO 63121 USA Donald Danforth Plant Science Center St Louis MO 63132 USA

While it is known that patatin-related phospholipase A （pPLA） activity is rapidly activated within 3min by auxin, hardly anything is known about how this signal influences downst.eam responses like transcription of early auxin-induced genes or other physiological responses. We screened mutants with T-DNA insertions in members of the pPLA gene family for molecular and physiological phenotypes related to auxin. Only one in nine Arabidopsis thaliana ppla knockdown mutants displayed an obvious const.tutive auxin-related phenotype. Compared to wild-type, ppla-IIlδ mutant seedlings had decreased main root lengths and increased lateral root numbers. We test.d auxin-induced gene expression as a molecular readout for primary molecular auxin responses in nine ppla mutants and found delayed up- regulation of auxin-responsive gene exRression in all of themL Thirty minutes after auxin treatment, up-regulation of up to 40% of auxin-induced genes was delayed in mutant seedlings. We observed only a few cases with hypersensitive auxin-induced gene expression in ppla mutants. While, in three ppla mutants, which were invest.gated in detail, rapid up- regulation （as early as 10 min after auxin st.mulus） of auxin-regulated genes was impaired, late transcriptional responses were wild-type-like. This regulatory or dynamic phenotype was consist.ntly observed in different ppla mutants with delayed up-regulation that frequently affected the same genes. This defect was not affected by pPLA transcript levels which remained const.nt. This indicates apost.ranslational mechanism as a functional link of pPLAs to auxin signaling. The need for a receptor triggering an auxin response without employing transcription regulation is discussed.

关键词： hormonal regulation hormone biology signal transduction.

Dynamic responses ofPAto environmental st.muli imaged by a genetically encoded mobilizable fluorescent sensor

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Plant Communications 2023年第3期4卷 99-111页

作者： Teng Li Xingkai Xiao Qingyun Liu Wenyan Li Li Li Wenhua Zhang Teun Munnik xuemin wang Qun Zhang College of Life Sciences State Key Laboratory of Crop Genetics and Germplasm EnhancementNanjing Agricultural UniversityNanjing 210095China Inst.tute of Botany Jiangsu Province and Chinese Academy of Sciences(Nanjing Botanical Garden Mem.Sun Yat-Sen)Nanjing 210014China Clust.r Green Life Sciences Section Plant Cell BiologySwammerdam Institute for Life SciencesUniversity of AmsterdamAmsterdamthe Netherlands Department of biology University of Missouri-St.LouisSt.LouisMO 63121USA Donald Danforth Plant Science Center St.LouisMO 63132USA

Membrane fluidity,permeability,and surface charges are controlled by phospholipid metabolism and *** the importance of phosphatidic acid(PA)as a bioactive molecule,the mechanical properties of PA translocation and subcellular accumulation are ***,we used a mobilizable,highly responsive genetically encoded fluorescent indicator,green fluorescent protein(GFP)-N160RbohD,to monitor PA dynamics in living *** majority of GFP-N160RbohD accumulated at the plasma membrane and sensitively responded to changes in PA ***,pharmacological,and genetic analyses illust.ated that both salinity and abscisic acid rapidly enhanced GFP-N160RbohD fluorescence at the plasma membrane,which mainly depended on hydrolysis of phospholipase *** contrast.heat st.ess induced nuclear translocation of PA indicated by GFP-N160RbohD through a process that required diacylglycerol kinase activity,as well as secretory and endocytic ***,we showed that gravity triggers asymmetric PA dist.ibution at the root apex,a response that is suppressed by PLDz2 *** broad utility of the PA sensor will expand our mechanist.c underst.nding of numerous lipid-associated physiological and cell biological processes and facilitate screening for protein candidates that the synthesis,transport,and metabolism of PA.

关键词： phospholipid phosphatidic acid RbohD heat st.ess salinity abscisic acid

Genome-and transcriptome-wide association st.dies provide insights into the genetic basis of natural variation of seed oil content in Brassica napus

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molecular Plant 2021年第3期14卷 470-487页

作者： Shan Tang Hu Zhao Shaoping Lu Liangqian Yu Guofang Zhang Yuting Zhang Qing-Yong Yang Yongming Zhou xuemin wang Wei Ma Weibo Xie Liang Guo National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural UniversityWuhanChina Hubei Key Laboratory of Agricultural Bioinformatics College of InformaticsHuazhong Agricultural UniversityWuhanChina Department of biology University of Missouri-St.LouisSt.LouisMO 63121USA Donald Danforth Plant Science Center St.LouisMO 63132USA School of Biological Sciences Nanyang Technological UniversitySingapore 637551Singapore

Seed oil content(SOC)is a highly important and complex trait in oil ***,we decipher the genetic basis of natural variation in SOC of Brassica napus by genome-and transcriptome-wide association st.dies using 505 inbred *** mapped reliable quantitative trait loci(QTLs)that control SOC in eight environments,evaluated the effect of each QTL on SOC,and analyzed selection in QTL regions during ***-hundred and ninety-two genes and four gene modules significantly associated with SOC were identified by analyzing population transcriptomes from seeds.A gene prioritization framework,POCKET(prioritizing the candidate genes by incorporating information on knowledge-based gene sets,effects of variants,genome-wide association st.dies,and transcriptome-wide association st.dies),was implemented to determine the causal genes in the QTL regions based on multi-omic datasets.A pair of homologous genes,BnPMT6s,in two QTLs were identified and experimentally demonst.ated to negatively regulate *** st.dy provides rich genetic resources for improving SOC and valuable insights toward underst.nding the complex machinery that directs oil accumulation in the seeds of *** and other oil crops.

关键词： Brassica napus seed oil content QTL GWAS TWAS gene module