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Eco-physiological characteristics of Tetracentr.n sinense *** in response to differ.nt light intensities

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Journal of Forestry research 2024年第3期35卷 29-44页

作者： r.ng Wang Xueheng Lu Hongyan Han xuemei Zhang Yonghong Ma Qinsong Liu Xiaohong Gan Key Laboratoryof Southwest Wildlife resources Conservation Ministry of EducationChina West Normal UniversityNanchong 637009People's Republic of China Institute of Plant Adaptation and Utilization in Southwest Mountain China West Normal UniversityNanchong 637009People's Republic of China Office of the People's Government of Yuexi County Yuexi County 616650Liangshan Yi Autonomous PrefecturePeople's Republic of China

The regeneration of Tetracentr.n sinense *** poor in the understory and in open areas due to the charac-teristics of natural regeneration of the species on forest edges and in *** is unclear whether differ.nt light intensities in various habitats affect eco-physiological characteristics of saplings and their.natural *** this study,the light intensity in *** habitats was simulated by artificial shading(L1:100%nS(natural sunlight)in the open;L2:50%nS in a forest gap or edge;L3:10%nS in understory)to investigate differ.nces in morphology,leaf structure,physiology,and photosynthesis of 2-year-old sap-lings,and to analyze the mechanism of light intensity on sapling *** differ.nces were observed in morphology(including leaf area,and specific leaf area)under differ.nt light *** to L1 and L3,chloroplast structure in L2 was *** increasing time,superoxide dismutase(SOD)and catalase(CAT)activities in L2 became gradually higher than under the other light intensities,while malondialdehyde(MDA)content was *** decreased osmoregulation substance contents of leaves but increased *** results suggest that light intensities significantly affect the eco-physiological characteristics of *** saplings and they would respond most favorably at intermediate levels of light by optimizing eco-physiological ***,50%natural sunlight should be created to promote saplings establishment and population recovery of *** dur.ng in situ conservation,including sowing mature seeds in forest edges or gaps and providing appropriate shade protection for seedlings and saplings in the open.

关键词： Chloroplast ultrastructure Eco-physiological characteristics Light intensities Sapling establishment Tetracentr.n sinense Oliv

Epidemiological Characterization of Dengue Fever—Yunnan Province,China,2010-2021

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China CDC weekly 2024年第20期6卷 457-462,I0002-I0007页

作者： Xiaoxiang Zhu Songwang Wang Yanfei Li Yu Cao xuemei Su Xiaotao Zhao national Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases Chinese Center for Disease Control and PreventionBeijingChina Yunnan Institute of Parasitic Diseases Pu’er CityYunnan ProvinceChina

Objective:The goal of this study is to analyze the epidemiological patterns of dengue fever across differ.nt districts and counties in Yunnan Province from 2010 to ***:In this study,we employed joinpoint regression analysis,spatial autocorrelation analysis,and space-time scan analysis to illustrate the spatiotemporal propagation and demographic influence of dengue fever,using both graphical and tabular presentations to clearly demonstrate the ***:Yunnan Province reported 14,098 cases of dengue fever dur.ng the period from 2010 to *** these,11,513 cases were caused by local tr.nsmission,2,566 were imported internationally,and 19 were inter-provincial *** tr.nds emerged,revealing a surge in incidences dur.ng the summer and autumn *** sex ratio of male to female cases was 1:0.88,with a significant majority of 82.00%of cases involving individuals belonging to the age group of *** service workers constituted the most impacted occupational group,forming 20.96%of total cases.A spatio-temporal scan identified significant cluster.ng of dengue fever cases across space and time,with the most pr.nounced cluster observed in southern Yunnan,primarily between 2015 and ***:Dengue fever in Yunnan Province manifests as biennial outbreaks,underscor.ng the necessity for increased surveillance,particularly in counties border.ng other regions.

关键词： counties district revealing

A metabolomics approach for monitor.ng thermal processing temperature of bovine milk using ultra-performance liquid chromatography tandem Q-Exactive mass spectrometry and multivariate data analysis

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未来食品学报(英文) 2024年第1期4卷 83-90页

作者： Yun Xie xuemei nie Chang Wang Xiuli Xu Feng Zhang Institute of Food Safety Chinese Academy of Inspection and QuarantineBeijing 100176China Key Laboratory of Food Quality and Safety for State Market regulation Chinese Academy of Inspection&QuarantineBeijing 100176China

Due to the lack of effective assessment method,overheated milk commodities are often marketed as pasteurized milk on the market,which was sold in high price by ***,this article aims to establish an approach based on metabolomics to monitor thermal processing temperature of bovine *** data of bovine milk samples heated at temperatures r.nging from 60℃to 150℃were achieved by ultra-performance liquid chromatography-high-resolution mass spectrometry(UPLC-HrMS)platform,followed by multivariate data analysis.A regular variation pattern of chemical composition as temperature rises was pictured,furthermore.n^(∈)carboxymethyl lysine(CML),n^(∈)-carboxyethyl lysine(CEL),*** and lysinoalanine(LAL)were identified as 5 of the most contributed compounds to discriminate pasteurized and ultra-high-temperature(UHT)*** the comprehensive study on their content changes,we concluded that the optimal temperature r.nge was 90-100℃for the generation of CML and CEL in this experiment,moreover.110~120℃for LAL.S0~100℃for pentosidine and 130-140℃for ***,a predicted rule to discriminate pasteurized and UHT milk was preliminarily established based on the ratios of CML/***/pentosidine and CML/*** could be applied in food labelling authentication of commercial bovine milk after further validation.

关键词： Metabolomics Heat treatment Bovine milk Pasteurization Utra-high-temperature

Diverse nucleotide substitutions in rice base editing mediated by novel TadA variants

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Plant Communications 2024年第8期5卷 104-112页

作者： Man Yu Yongjie Kuang Chenyang Wang xuemei Wu Shaofang Li Dawei Zhang Wenxian Sun Xueping Zhou Bin r.n Huanbin Zhou State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193China Department of Plant Pathology China Agricultural UniversityBeijing 100193China Scientific Observing and Experimental Station of Crop Pests in Guilin Ministry of Agriculture and Rural AffairsGuilin 541399China Ministry of Education Key Laboratory for Bio-resource and Eco-Envir.nment College of Life ScienceState Key Laboratory of Hydraulics and Mountain River EngineeringSichuan UniversityChengdu 610064China State Key Laboratory of Vegetable Biobreeding National Engineering Research Center for VegetablesBeijing Vegetable Research CenterBeijing Academy of Agriculture and Forestry ScienceBeijing 100097China State Key Laboratory of rice Biology Institute of BiotechnologyZhejiang UniversityHangzhou 310058China Key Laboratory of Gene Editing Technologies(Hainan) Ministry of Agricultural and Rural AffairsSanya 572024China

CrISPr-mediated base editors have been widely used to correct defective alleles and create novel alleles by artificial evolution for the rapid genetic improvement of *** editing capabilities of base editors strictly rely on the performance of various nucleotide modification *** with the welldeveloped adenine base editors(ABEs),cytosine base editors(CBEs)and dual base editors suffer from unstable editing efficiency and patterns at differ.nt genomic loci in rice,significantly limiting their ***,we comprehensively examined the base editing activities of multiple evolved TadA8e variants in *** found that both TadA-CDd and TadA-E27r.n46L achieved more robust C-to-T editing than previously reported hyperactive hAID*D,and TadA-CDd outperformed TadA-E27r.n46L.A C-to-G base editor(CGBE)engineered with TadA-CDd and OsUnG performed highly efficient C-to-G editing in rice compared with that of *** addition,a dual base editor constructed with a single protein,TadDE,enabled simultaneous,highly efficient C-to-T and A-to-G editing in ***,our results demonstrate that TadA8e derivatives improve both CBEs and dual base editors in rice,providing a powerful way to induce diverse nucleotide substitutions for plant genome editing.

关键词： CrISPr TadA variants cytosine base editing dual base editor rice

Enhanced SErS aptasensor for sensitive detection of saxitoxin based on HAuCl/HOreaction system and Pt/Au nanozyme

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Food Science and Human Wellness 2024年

作者： Yueqing Li xuemei Tang Wei Zeng Ting Wu Long Wu School of Food Science and Engineer.ng， Key Laboratory of Tropical Fruits and Vegetables Quality and Safety for State Market regulation， Hainan University

Saxitoxin （STX） is a mar.ne biotoxin produced by cyanobacteria and methanobacteria， which is harmful to humans and aquatic organisms. Therefore， it is urgent to establish an efficient， sensitive and selective method for the screening of STX. In this paper， based on the HAuCl4/H2O2reaction system， gold nanoparticles （Au nPs） decorated Fe3O4magnetic beads (Fe3O4@Au nPs) and Pt/Au nanozymes （Pt/Au nZs）， an enhanced SErS aptasensor was proposed for sensitive and selective detection of STX. Firstly， SErS tag （4-MBA） and aptamer modified Fe3O4@Au nPs were hybridized with complementary DnA modified Pt/Au nZs （Pt/Au-cDnA） to form the specific complexes. Wherein， aptamer acted as capture agent to specifically recognize STX， Pt/Au nZs catalyzed HAuCl4/H2O2reaction to enhance SErS signal， and Fe3O4facilitated the magnetic separation and simple detection operations. The SErS aptasensor achieved sensitive detection of STX with a wide linear r.nge from 0.02 μmol/L to 0.8 μmol/L and a low detection limit of 13 nmol/L （LOD， S/n=3）. To evaluate the performance of this method， STX were detected in the spiked seafood samples with recoveries from 78.75% to 150% with relative errors below 6.52%. Besides， the SErS aptasensor behaved good stability and repeatability， revealing its promising potential in STX detection and food safety detection.

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An optimized high-throughput SArS-CoV-2 dual reporter tr.ns-complementation system for antiviral screening in vitro and in vivo

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Virologica Sinica 2024年第3期39卷 447-458页

作者： Yingjian Li Xue Tan Jikai Deng xuemei Liu Qianyun Liu Zhen Zhang Xiaoya Huang Chao Shen Ke Xu Li Zhou Yu Chen State Key Laboratory of Virology RNA InstituteCollege of Life Sciences and Frontier Science Center for Immunology and MetabolismWuhan UniversityWuhan430072China Institute for Vaccine research at Animal Bio-safety Level III Laboratory Wuhan University School of MedicineWuhan430071China

The severe acute respiratory syndrome cor.navirus 2(SArS-CoV-2)is still epidemic around the *** manipulation of SArS-CoV-2 is restricted to biosafety level 3 laboratories(BSL-3).In this study,we developed a SArS-CoV-2Δn-GFP-HiBiT replicon delivery particles(rDPs)encoding a dual reporter gene,GFP-HiBiT,capable of producing both GFP signal and luciferase *** optimal selection of the reporter gene,GFP-HiBiT demonstrated superior stability and convenience for antiviral ***,we established a rDP infection mouse model by deliver.ng the n gene into K18-hACE2 KI mouse through *** mouse model supports rDP replication and can be utilized for in vivo antiviral *** summary,the rDP system serves as a valuable tool for efficient antiviral screening and studying the gene function of ***,this system can be manipulated in BSL-2 laboratories,decreasing the threshold of experimental requirements.

关键词： SArS-CoV-2 rDP Antiviral evaluation Mouse model BSL-2

Overexpression of the tr.nscription factor MdWrKY115 improves drought and osmotic stress toler.nce by directly binding to the MdrD22 promoter in apple

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Horticultural Plant Journal 2024年第3期10卷 629-640页

作者： Qinglong Dong Yi Tian xuemei Zhang Dingyue Duan He Zhang Kaiyu Yang Peng Jia Haoan Luan Suping Guo Guohui Qi Ke Mao Fengwang Ma College of Forestry Hebei Agricultural UniversityBaodingHebei 071001China national Engineer.ng research Center for Agriculture in northern Mountainous Areas Agricultural Technology Innovation Center in Mountainous Areas of Hebei ProvinceHebei Agricultural UniversityBaodingHebei 071001China State Key Laboratory of Crop Stress Biology for Arid Areas Shaanxi Key Laboratory of AppleCollege of HorticultureNorthwest A&F UniversityYanglingShaanxi 712100China College of Horticulture Hebei Agricultural UniversityBaodingHebei 071001China

Abiotic stress reduces plant yield and *** tr.nscription factors play key roles in abiotic stress responses in plants,but the molecular mechanisms by which WrKY tr.nscription factors mediate responses to drought and osmotic stresses in apple(Malus×domestica Borkh.)remain ***,we functionally characterized the apple GroupⅢWrKY gene ***-PCr analysis showed that MdWrKY115 expression was up-regulated by drought and osmotic *** activity analysis revealed that the promoter activity of MdWrKY115 was enhanced under osmotic *** localization and tr.nsactivation assays indicated that MdWrKY115 was localized to the nucleus and had a tr.nscriptional activity domain at the n-terminal *** analysis revealed that the overexpression of MdWrKY115 in Arabidopsis plants and in apple callus markedly enhanced their toler.nce to drought and osmotic *** affinity purification sequencing showed that MdWrKY115 binds to the promoter of the stress-related gene *** binding was further verified by an electrophoretic mobility shift ***,these findings suggest that MdWrKY115 is an important regulator of osmotic and drought stress toler.nce in apple.

关键词： Apple MdWrKY115 Osmotic stress Drought stress Function analysis

Impact of metabolic dysfunction-associated steatotic liver disease on the efficacy of immunotherapy in patients with chr.nic hepatitis B-related hepatocellular carcinoma

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Cancer Biology & Medicine 2024年第9期21卷 813-825页

作者： Jiaxin Han Wentao Kuai Liu Yang xuemei Tao Yuekui Wang Minghui Zeng Yuqin Li Yuqiang Mi ningning Zhang Wei Lu Liang Xu Clinical School of the Tianjin Second People's Hospital Tianjin Medical UniversityTianjin 300192China Department of Hepatology Tianjin Second People's HospitalTianjin 300192China Tianjin Institute of Hepatology Tianjin 300192China Department of Hepatobiliary Oncology Liver Cancer CenterTianjin Medical University Cancer Institute&HospitalNational Clinical Research Center for CancerKey Laboratory of Cancer Prevention and TherapyTianjinTianjin's Clinical Research Centerfor CancerTianjin300060China

Objective:To investigate the impact of metabolic dysfunction-associated steatotic liver disease(MASLD)on the efficacy of immune checkpoint inhibitor(ICI)-based therapy in patients with chr.nic hepatitis B(CHB)-related hepatocellular carcinoma(HCC).Methods:A total of 155 patients with CHB-related HCC who received ICI–based therapy(in the Department of Hepatology,Tianjin Second People’s Hospital and Department of Hepatobiliary Oncology,Tianjin Medical University Cancer Institute&Hospital)between April 2021 and December 2023 were *** were divided into two groups:MASLD concurr.nt with CHB[MASLD-CHB](n=38),and CHB(n=117).results:The median progression-free survival(PFS,6.9 months vs.9.3 months;P=0.001),progressive disease(57.89%vs.37.61%;P=0.028),and disease control rate(42.11%vs.62.39%;P=0.028)in the MASLD-CHB group were significantly worse than the CHB *** median overall survival was not *** percentage of CD4+PD1+(17.56%vs.8.89%;Pnd CD8+PD1+T cells(10.50%vs.7.42%;P=0.005)in patient samples from the MASLD-CHB group were significantly higher than the CHB *** MASLD[hazard ratio(Hr)=1.921;95%CI,1.138–3.245;P=0.015]and alpha-fetoprotein levels after 3 months of treatment(Hr=2.412;95%CI,1.360–4.279;P=0.003)were independent risk factors for PFS in all ***:ICI-based therapy in patients with CHB-related HCC and concurr.nt MASLD resulted in poorer efficacy and shorter PFS compared to patients with CHB-related HCC alone.

关键词： Metabolic dysfunction-associated steatotic liver disease chr.nic hepatitis B hepatocellular carcinoma immunotherapy efficacy

nitrogen addition promotes soil carbon accumulation globally

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Science China Life Sciences 2024年

作者： xuemei Yang Suhui Ma Erhan Huang Danhua Zhang Guoping Chen Jiangling Zhu Chengjun Ji Biao Zhu Lingli Liu Jingyun Fang Institute of Ecology College of Urban and Environmental Sciences and Key Laboratory for Earth Surface Processes of the Ministry of Education Peking University Ministry of Education Key Laboratory for Tr.nsboundary Ecosecurity of Southwest China School of Ecology and Environmental Science Yunnan University State Key Laboratory of Vegetation and Envir.nmental Change Institute of Botany Chinese Academy of Sciences

Soil is the largest carbon (C) reservoir in terrestrial ecosystems and plays a crucial role in regulating the global C cycle and climate *** nitrogen (n) deposition has been widely considered as a critical factor affecting soil organic carbon (SOC) storage, but its effect on SOC components with differ.nt stability remains unclear. Here, we analyzed extensive empirical data from 304 sites worldwide to investigate how SOC and its components respond to n addition. Our analysis showed that n addition led to a significant increase in bulk SOC(6.7%), with greater increases in croplands (10.6%) and forests (6.0%) compared to grasslands (2.1%). regarding SOC components, n addition promoted the accumulation of plant-derived C (9.7%–28.5%) over microbial-derived C (0.2%), as well as labile (5.7%) over recalcitr.nt components (–1.2%), resulting in a shift towards increased accumulation of plant-derived labile C. Consistently, n addition led to a greater increase in particulate organic C (11.9%) than mineral-associated organic C (3.6%), suggesting that n addition promotes C accumulation across all pools, with more increase in unstable than stable pools. The responses of SOC and its components were best predicted by the n addition rate and net primary productivity. Overall, our findings suggest that n enrichment could promote the accumulation of plant-derived and non-mineral associated C and a subsequent decrease in the overall stability of soil C pool, which underscores the importance of consider.ng the effects of n enrichment on SOC components for a better understanding of C dynamics in soils.

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