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Characteristics and prospect of geothermal industry in China under the“dual carbon”target

Energy Geoscience 2023年第4期4卷 171-177页

作者： Zuoxian luo xuemei lu Sinopec Economics&Development Research Institute BeijingChina Sinopec Petroleum Exploration and Development Research Institute BeijingChina

Geothermal energy utilization in China had been promoted actively during the 13^(th)Five-Year Plan period(2016-2020),with resultant achievements showing unique Chinese ***(measured by areas)heated/cooled by intermediate-to-deep geothermal energy increased more rapidly than those by shallow geothermal *** power generation was restricted by the distribution of the resource and the sufficiency of local power *** the proposition of“dual carbon goal”by the central government of China and the coming era of great development of renewable energy,geothermal energy will be provided more development opportunities in room heating/cooling and power generation in the country ever than ***,tackling technology bottlenecks and introduction of appropriate policy support are still urgently needed for nurturing a healthy environment for geothermal *** suggests that a near-to medium-term development of geothermal energy shall be focused on a comprehensive and efficient utilization both through heating/cooling and power *** heating/cooling by intermediate-to-deep and shallow geothermal energy shall be promoted nationwide according to local resource endowment and economic *** power generation in Yunnan and Tibet shall be pushed forward in an orderly *** medium-and long-term development,focus shall be put on power generation by using low to medium temperature geothermal energy and hot dry ***"hot spring+"model shall be adapted in steps on a regular *** achieve these,measures including attaching importance to tackling key technologies,promoting standards for geothermal energy development and utilization,strengthening geothermal energy exploration and evaluation,and seeking for necessary policy support shall be taken.

关键词： Geothermal industry Post-Covid 19 era Geothermal heating/cooling Geothermal power Policy support

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A novel thermophilic endoglucanase from a mesophilic fungus Fusarium oxysporum

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Chinese Science Bulletin 2006年第2期51卷 191-197页

作者： LIU Shuyan DUAN Xinyuan lu xuemei GAO Peiji State Key Laboratory of Microbial Technology Shandong University Ji'nan 250100 China

A novel thermophilic endoglucanase (EGt) was extracted from a mesophilic fungus (Fusarium oxysporum L19). We invoked conventional kinetic enzyme reactions using the sodium salt of carboxymethyl cellulose (CMC-Na) as substrate. EGt displayed optimal activity at 75℃ when kept running 30 min in the temperature range of 30―85℃. Thermal stability curve measured at 70℃ suggested that its half-life time is 15.1 min. The activity was enhanced in the presence of Co2+ or Mg2+ but inhibited by Pb2+ and Fe3+. Moreover, N-bromosuccinimide (NBS) modification resulted in a complete loss of EGt activity, suggesting that tryptophan residues may be involved in the enzyme active site. Amino acid composition analysis demonstrated that EGt contains more proline residues. EGt lacks activity towards p-nitro- phenyl cellobiose (pNPC). The N-terminal amino acid se- quence of EGt is SYRVPAANGFPNPDASQEKQ, and the gene of EGt was sequenced and analyzed. Extensive se- quence alignments failed to show any homology between EGt and any known endoglucanases. This is the first report addressing the thermal adaptation of a cellulolytic enzyme from the mesophilic fungus F. oxysporum. Maybe the ex- pression of multiple isoenzyme in an organism helps it adapt to complex living environments.

关键词：嗜温菌尖镰孢香蕉枯萎病嗜热葡聚糖内切酶热稳定性热活性

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Purification and characteristics of a low-molecular-weight peptide possessing oxidative capacity for phenol from Phanerochaete chrysosporium

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Science China(Life Sciences) 2006年第3期49卷 243-250页

作者： HU Ming ZHANG Weican lu xuemei GAO Peiji State Key Laboratory of Microbial Technology Shandong University Jinan 250100 China State Key Laboratory of Microbial Technology Shandong University Jinan 250100 China State Key Laboratory of Microbial Technology Shandong University Jinan 250100 China State Key Laboratory of Microbial Technology Shandong University Jinan 250100 China

A new low-molecular-weight peptide with phenol oxidase activity, named Pc factor, was isolated; purified from liquid culture of a white-rot basidiomycete Phanerochaete chrysosporium. Its molecular weight was about 600 Da estimated by gel-filtration. Three amino acids Glu, Gly; Val were detected in hydrolysate. Absorption peaks corresponding to amino acids; peptide were observed by UV; IR spectra analysis. And the signal of Cα of amino acid was also detected by [13]C-NMR method. Pc factor had high thermostability; remained active in weakly alkalescent pH range. It could chelate Fe[3+]; reduce it to Fe[2+], but no hydroxyl radical HO˙ could be detected during the reaction process. It could oxidize phenolic lignin-model compounds such as 2,6-dimethoxyphenol (2,6-DMP), 2,2′-azinobis (3-ethylbenzathiazoline-6-sulfinic acid) (ABTS); syringaldazine in the absence of Mn[2+]; H2O2. These characteristics differed greatly from those of manganese peroxidases. The oxidative catalysis of Pc factor can be enhanced by certain metal ions such as Cu[2+]; Mn[2+] etc.,; O2 molecule was necessary for this reaction. In summary, Pc factor may function as an electron carrier in this novel oxidation-reduction system.

关键词： Phanerochaete chrysosporium, Pc factor, purification, phenol oxidase activity, lignin.

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On the founder effect in COVID-19 outbreaks:how many infected travelers may have started them all?

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National Science Review 2021年第1期8卷 30-39页

作者： Yongsen Ruan Zhida luo Xiaolu Tang Guanghao Li Haijun Wen Xionglei He xuemei lu Jian lu Chung-I Wu State Key Laboratory of Biocontrol School of Life Sciences Sun Yat-sen University State Key Laboratory of Protein and Plant Gene Research Center for Bioinformatics School of Life SciencesPeking University CAS Key Laboratory of Genomic and Precision Medicine Beijing Institute of Genomics Chinese Academy of Sciences State Key Laboratory of Genetic Resources and Evolution Kunming Institute of ZoologyCenter for Excellence in Animal Evolution and Genetics Chinese Academy of Sciences

How many incoming travelers（I;at time 0, equivalent to the ‘founders’ in evolutionary genetics） infected with SARS-CoV-2 who visit or return to a region could have started the epidemic of that region? I;would be informative about the initiation and progression of epidemics. To obtain I;, we analyze the genetic divergence among viral populations of different regions. By applying the ‘individual-output’ model of genetic drift to the SARS-CoV-2 diversities, we obtain I;lusion is robust regardless of the source population, the continuation of inputs（I;for t > 0） or the fitness of the variants. With such a tiny trickle of human movement igniting many outbreaks, the crucial stage of repressing an epidemic in any region should,therefore, be the very first sign of local contagion when positive cases first become identifiable. The implications of the highly ‘portable’ epidemics, including their early evolution prior to any outbreak, are explored in the companion study（Ruan et al., personal communication）.

关键词： SARS-CoV-2 COVID-19 population genetics genetic drift founder effect

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Synergistic effects of cellobiose dehydrogenase and manganese-dependent peroxidases during lignin degradation

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Chinese Science Bulletin 2001年第23期46卷 1956-1962页

作者： HUANG Feng FANG Jing lu xuemei GAO Peiji CHEN Jiaxiang State Key Laboratory of Microbial Technology Shandong UniversityJinan 250100China State Key Lab of Pulp and Paper Engineering South China University of TechnologyGuangzhou 510641China

The synergistic effects of cellobiose dehydro-genase (CDH) and manganese-dependent peroxidases (MnP) on the degradation of kraft pulp cellulolytic enzyme lignin (CEL) were investigated. Addition of CDH significantly increased the amount of water-soluble products reduced from CEL by MnP. CDH facilitated the reduction of the contents of methoxyl, carboxyl, phenolic hydroxyl and total hydroxyl groups of CEL by MnP. 1H-NMR analysis showed that addition of CDH also decreased further the amount of protons of CEL degraded by MnP. The results proved for the first time that CDH could promote degradation of lignin by MnP and suggest that CDH could not only promote degradation of cellulose but also is an important part of the lignin biodeg-radation system.

关键词： cellobiose dehydrogenase manganese-dependent per- oxidase lignin degradation.

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On the epicenter of COVID-19 and the origin of the pandemic strain

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National Science Review 2023年第4期10卷 21-23页

作者： Yongsen Ruan Haijun Wen Mei Hou Weiwei Zhai Shuhua Xu xuemei lu State Key Laboratory of Biocontrol School of Life Sciences Sun Yat-sen University Institute of Zoology Chinese Academy of Sciences School of Life Sciences Fudan University State Key Laboratory of Genetic Resources and Evolution Yunnan Key Laboratory of Biodiversity Information Kunming Institute of Zoology Chinese Academy of Sciences

Ruan et al.(2022) published in National Science Review(NSR) an analysis that concludes two early centers in the spread of COVID-19 [1]. Since the strain(referred to as DG1111) that spread globally to cause the pandemi...

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The use of SARS-CoV-2-related coronaviruses from bats and pangolins to polarize mutations in SARS-Cov-2

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Science China(Life Sciences) 2020年第10期63卷 1608-1611页

作者： Tao Li Xiaolu Tang Changcheng Wu Xinmin Yao Yirong Wang xuemei lu Jian lu State Key Laboratory of Genetic Resources and Evolution Kunming Institute of ZoologyCenter for Excellence in Animal Evolution and GeneticsChinese Academy of SciencesKunming 650223China State Key Laboratory of Protein and Plant Gene Research Center for BioinformaticsSchool of Life SciencesPeking UniversityBeijing 100871China

Dear Editor,The coronavirus disease 2019(COVID-19)caused by the SARS-CoV-2 coronavirus has become a global *** SARS-CoV-2 genome has a similarity of 96.2%to that of RaTG13,a bat SARS-CoV-2-related coronavirus detected in Rhinolophus affinis(Paraskevis et al.,2020;Zhou et al.,2020).The SARS-CoV-2 genome also has 85.5%−92.4%sequence similarity to SARS-CoV-2-related coronaviruses from Malayan pangolins that have been seized in anti-smuggling operations in southern China(Guangdong-Pangolin(GD-Pangolin-CoV)and Guangxi-Pangolin(GX-Pangolin-CoV)genomes)(Liu et al.,2019;Lam et al.,2020).

关键词： al. similarity operations

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SPI in Myanmar

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China Oil & Gas 2023年第4期30卷 38-46页

作者： lu xuemei

Myanmar-China Oil and Gas Pipeline Project The Myanmar-China Oil and Gas Pipeline Project,jointly invested by 6 companies from 4 countries (China,Myanmar,South Korea,and India),is an international cooperation *** Project involves the MyanmarChina Oil Pipeline,Myanmar-China Gas Pipeline,and oil *** Myanmar-China Oil Pipeline is jointly invested and constructed by China National Petroleum Corporation(CNPC) and Myanma Oil and Gas Enterprise (MOGE),

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SPI in Central and Southeast Asia

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China Oil & Gas 2023年第4期30卷 18-46页

作者： lu xuemei 不详

SPI in Kazakhstan SPI participated in the construction and operation of four oil and gas pipeline projects in Kazakhstan,and they are Kazakhstan-China Gas Pipeline,Beineu-Shymkent Gas Pipeline,Kazakhstan-China Crude Oil Pipeline and MunaiTas North-West Crude Oil *** pipeline operation,four joint venture companies were set up for the four pipeline projects respectively.

关键词： companies venture SPI

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Trend of Intelligent Refinery Development

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China Oil & Gas 2023年第5期30卷 64-67页

作者： lu xuemei Sinopec Petroleum Exploration and Production Research Institute

Digital transformation is a general trend in global industrial development,and an important part of the new generation of energy and industrial *** the fourth industrial revolution represented by the deep integration of new-generation information technology with various sectors of the economy and society develops rapidly,digitalization,networking,and intellectualization are reshaping the global social,political,and economic *** global information revolution represented by the Internet industrialization and industry intellectualization is silently changing the patterns of energy production and consumption.

关键词： revolution intellectual Trend

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