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Transcriptional regulation of MdPIN7 by MdARF19 during gravityinduced formation of adventitious root GSA in self-rooted apple stock

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Horticultural Plant Journal 2024年第5期10卷 1073-1084页

作者： Zenghui Wang xuemei Yang Linyue Hu Wei Liu Lijuan Feng Xiang shen Yanlei Yin Jialin Li Shandong Institute of Pomology Tai'anShandong 271000China School of Biological Science and Technology University of JinanJinanShandong 250022China State Key Laboratory of Crop Biology Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(Huanghuai Region)Ministry of AgricultureCollege of Horticulture Science and EngineeringShandong Agricultural UniversityTai'anShandong 271018China

Self-rooted apple stock is widely used for apple ***,the shallowness of the adventitious roots in self-rooted apple stock leads to poor performance in the barren orchards of *** is because of the considerable difference in the development of a gravitropic set-point angle(GSA)between self-rooted apple stock and seedling ***,it is crucial to study the molecular mechanism of adventitious root GSA in self-rooted apple stock for breeding self-rooted and deep-rooted apple rootstock *** apple auxin response factor MdARF19 functioned to establish the adventitious root GSA of self-rooted apple stock in response to gravity and auxin ***19 bound directly to the MdPIN7 promoter,activating its transcriptional expression and thus regulating the formation of the adventitious root GSA in 12-2 self-rooted apple ***,MdARF19 influenced the expression of auxin efflux carriers(MdPIN3 and MdPIN10)and the establishment of adventitious root GSA of self-rooted apple stock in response to gravity signals by direct activation of *** findings provide new information on the transcriptional regulation of MdPIN7 by auxin response factor MdARF19 in the regulation of the adventitious root GSA of self-rooted apple stock in response to gravity and auxin signals.

关键词： Apple Self-rooted stock Gravity MdARF19 MdPIN7 Gravitropic set-point angle Transcriptional regulation

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中药专业开设新药研究与开发课程的探索

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药学教育 2007年第4期23卷 18-20页

作者：周毅生沈雪梅陈求芳王芳黄嗣航廖华卫广东药学院中药学院广州510006

介绍在中药专业开设新药研究与开发课程的主要内容以及对学生课程学习过程进行的全程监控和考核。该课程主要是指导学生按国家食品、药品监督管理局有关中药、天然药物注册申报资料要求,独立完成一项新药的研究方案设计、实验研究和申... 详细信息

介绍在中药专业开设新药研究与开发课程的主要内容以及对学生课程学习过程进行的全程监控和考核。该课程主要是指导学生按国家食品、药品监督管理局有关中药、天然药物注册申报资料要求,独立完成一项新药的研究方案设计、实验研究和申报资料的撰写。本课程的开设有利于培养学生的创新能力以及综合运用专业知识能力,独立分析问题和解决问题能力。

关键词：中药学专业新药研究与开发课程创新能力

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枯草芽孢杆菌发酵工艺研究进展

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当代化工 2024年第8期53卷 1952-1958页

作者：汤维涛唐堂沈雪梅何太波刘海军武丽达谭剑中粮营养健康研究院有限公司北京102209 营养健康与食品安全北京市重点实验室北京102209 老年营养食品研究北京市工程实验室北京102209 吉林中粮生化有限公司玉米深加工国家工程研究中心吉林长春130033

综述了枯草芽孢杆菌发酵过程中涉及的多种发酵工程策略,详细探讨了包括高产菌株的选育、培养基的优化、发酵条件的设定以及发酵过程的精细调控等方面的内容。探讨了通过传统育种技术和现代基因工程手段来提高菌株产量的方法。分析了不... 详细信息

综述了枯草芽孢杆菌发酵过程中涉及的多种发酵工程策略,详细探讨了包括高产菌株的选育、培养基的优化、发酵条件的设定以及发酵过程的精细调控等方面的内容。探讨了通过传统育种技术和现代基因工程手段来提高菌株产量的方法。分析了不同营养成分和添加剂对菌株生长和产物生成的影响。介绍了温度、pH、溶氧量等因素对发酵过程的影响及其优化策略。此外,还探讨了发酵过程的动态监控和调控方法,以实现高效的生产过程。对枯草芽孢杆菌工程菌发酵领域的最新研究现状进行了全面介绍,总结了当前的研究成果和存在的问题,提出了未来研究的方向和可能的发展趋势。

关键词：枯草芽孢杆菌发酵优化基因工程菌高产菌株选育补料方式

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推动四川省农业产业化的战略措施探索

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中国农学通报 2005年第10期21卷 438-441页

作者：彭昌家江方明赵兴建何家国沈雪梅王明霞四川省南充市农业局南充637000 四川省南充市高坪区农业局南充637100

分析了四川省围绕农业办工业的必要性和存在的问题,以及围绕农业办工业的思路、原则、目标和重点,提出了围绕农业办工业,推动农业产业化的对策措施。

关键词：农业工业产业化战略农业产业化四川省工业

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“Reinforced concrete”-like flexible transparent electrode for organic solar cells with high efficiency and mechanical robustness

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Science China Chemistry 2022年第6期65卷 1164-1172页

作者： Yang Chen Juanyong Wan Guiying Xu Xiaoxiao Wu Xinqi Li Yunxiu shen Fu Yang xuemei Ou Yaowen Li Yongfang Li Laboratory of Advanced Optoelectronic Materials Suzhou Key Laboratory of Novel Semiconductor-optoelectronics Materials and DevicesCollege of ChemistryChemical Engineering and Materials ScienceSoochow UniversitySuzhou215123China State and Local Joint Engineering Laboratory for Novel Functional Polymeric Materials Jiangsu Key Laboratory of Advanced Functional Polymer Dsign and ApplicationSoochow UniversitySuzhou215123China Beijing National Laboratory for Molecular Sciences Institute of ChemistryChinese Academy of SciencesBeijing100190China

Flexible transparent electrodes(FTEs) with robust mechanical stability are crucial for the industrial application of flexible organic solar cells(OSCs). However, their production remains challenging owing to the difficulty in balancing the conductivity,transmittance, and adhesion of FTEs to substrates. Herein, we present the so-called “reinforced concrete” strategy which finetunes the structure of silver nanowires(Ag NWs)-based FTEs with polydopamine(PDA) possessing good adhesion properties and moderate reducibility. The PDA reduces Ag+to form silver nanoparticles(Ag NPs) which grow like “rivets” at the Ag NW junction sites;PDA stabilizes the Ag NW skeleton and improves the adhesion between the Ag NWs and polyethylene terephthalate(PET) substrate and interface layer. The obtained Ag NW:PDA:Ag NP FTE exhibits excellent optoelectronic properties and high mechanical stability. The resulting flexible OSCs exhibit 17.07% efficiency, high flexibility during 10,000 bending test cycles, and robust peeling stability. In addition, this “reinforced concrete”-like FTE provides great advantages for the production of large-area flexible OSCs, thereby paving a new way toward their commercial application.

关键词： flexible organic solar cells flexible transparent electrodes silver nanowires polydopamine mechanical stability

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基于“两态三三构型规律”理论浅析疏调气机汤加减降低CA724体会

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云南中医药大学学报 2023年第2期46卷 40-43页

作者：张成绩梁智蓉李治良申雪梅杨生丽刘建丽郭利华云南中医药大学云南昆明650500 重庆市大足区中医院重庆402360 玉溪市中医医院云南玉溪653199 云南省中医医院云南昆明650021

“两态三三构型规律”是国医大师张震对中医辨证论治理论及证型的拓展,亦是将理论与实践相联系的桥梁。运用“两态三三构型规律”,掌握临床思维,更加快捷简便准确确定证型,依据证型确定治法方药,提高了治病的准确性及效率。我们通过一... 详细信息

“两态三三构型规律”是国医大师张震对中医辨证论治理论及证型的拓展,亦是将理论与实践相联系的桥梁。运用“两态三三构型规律”,掌握临床思维,更加快捷简便准确确定证型,依据证型确定治法方药,提高了治病的准确性及效率。我们通过一则临床病案来阐述如何运用“两态三三构型规律”分析疏调气机汤加减降低CA724的理论及思维过程。

关键词：两态三三构型规律疏调气机汤肿瘤标志物CA724 中医药

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基于响应面法优化水提紫娟茶花青素工艺

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甘肃农业大学学报 2021年第1期56卷 175-181页

作者：张钎沈雪梅刘琨毅张纪伟周艺凡李光秀李家华云南农业大学龙润普洱茶学院云南昆明650201 昆明市农业科学研究院云南昆明650201 宜宾职业技术学院五粮液技术与食品工程学院四川宜宾644003 云南农业大学食品科学技术学院云南昆明650201 盐津县农业技术推广中心云南盐津657500

【目的】为了拓宽紫娟茶中花青素应用领域,建立环保、经济的紫娟茶花青素提取方法.【方法】以紫娟茶为试验原料,0.07%的柠檬酸溶液为酸化剂,纯净水为提取溶剂,在单因素试验的基础上,选择提取温度(A)、料液比(B)和提取时间(C)为影响因素... 详细信息

【目的】为了拓宽紫娟茶中花青素应用领域,建立环保、经济的紫娟茶花青素提取方法.【方法】以紫娟茶为试验原料,0.07%的柠檬酸溶液为酸化剂,纯净水为提取溶剂,在单因素试验的基础上,选择提取温度(A)、料液比(B)和提取时间(C)为影响因素,以浸提液中花青素质量浓度为响应值,采用Box-Behnken响应曲面试验法优化紫娟茶中花青素的提取工艺.【结果】建立了二次多项式回归模型方程:y=6.29+0.3581A-0.3186B-0.5376C-0.1125AB+0.2345AC-0.1304BC-0.7173A^(2)-0.1893B^(2)+0.0005C^(2)(R^(2)=0.8430),该模型拟合程度较好.因素影响大小排序为提取时间>提取温度>料液比,时间对水提紫娟茶花青素质量浓度高低的影响极为显著(P<0.01).水提紫娟茶花青素的最佳工艺条件为:料液比1∶32(M∶V)、提取温度71.3℃、提取时间12.5 min,在此条件下浸提液中花青素质量浓度可达6.908 mg/mL.【结论】研究结果为紫娟茶花青素水提方法提供了依据.

关键词：紫娟茶花青素响应面法工艺优化

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Hydrogen Inhalation Ameliorates Oxidative Stress and Glucose Metabolism Disorder in the Brain of Hindlimb Unloading Rats

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Space(Science & Technology) 2023年第1期3卷 214-223页

作者： Xiaoyan Jin Fei Xie Yang Yi Yating Zhang Shukuan Ling Qianwei shen xuemei Ma Yingxian Li State Key Laboratory of Space Medicine Fundamentals and Application China Astronaut Research and Training CenterBeijingChina Faculty of Environment and Life Beijing University of TechnologyBeijingChina National Institute on Drug Abuse National Institutes of HealthBaltimoreMDUSA

Many studies have shown that spaceflight causes oxidative stress and induces brain disorder in astronauts,but the counter measurements are *** evidence demonstrated that hydrogen can act as a therapeutic *** this study,rats were treated with or without about 5%hydrogen under hindlimb unloading or normal conditions for 28 *** assessed rat’s brain function by open-field test,step-down passive avoidance test,the neurotransmitter’s level detected by liquid chromatography with tandem mass spectrometry,and Nissl and hematoxylin-eosin staining *** also assessed the oxidative damage by changes of malondialdehyde level,the ratio of reduced glutathione to oxidized glutathione,and superoxide dismutase and catalase *** metabolism disorder was disclosed through glucose metabolomic *** underlying mechanism of the effects of hydrogen was analyzed by mRNA sequencing and detecting mRNA and protein *** data showed that hindlimb unloading caused oxidative damage and glucose metabolism disorder in brain tissues and decreased brain function in *** inhalation reduced oxidative damage,ameliorated glucose metabolism disorder,and alleviated the dysfunction of rat brain ***-proliferator-activated receptor gamma coactivator 1αand brain-derived neurotrophic factor,the key regulators of glucose metabolism and brain function,were obviously *** research confirms the protective effect of hydrogen inhalation on declining brain function under hindlimb unloading conditions and discloses the underlying mechanism,which provides a potential strategy for astronauts’health.

关键词： analysis metabolism damage

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Magnetic response to atmospheric heavy metal pollution recorded by dust-loaded leaves in Shougang industrial area, western Beijing

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Chinese Science Bulletin 2008年第10期53卷 1555-1564页

作者： HU ShouYun DUAN xuemei shen MingJie U BLAHA W ROESLER YAN HaiTao E APPEL V HOFFMANN State Key Laboratory of Lake Science and Environment Nanjing Institute of Geography and Limnology Chinese Academy of Sci- ences Nanjing 210008 China Graduate University of Chinese Academy of Sciences Beijing 100049 China epartment of Geography East China Normal University Shanghai 200062 China Institute for Geosciences University of Tubingen 72076 Tubingen Germany epartment of Geography Shanghai Normal-University Shanghai 200234 China

Fifty-five evergreen tree's leaves growing less than one year were collected from Shougang industrial area in western suburb of Beijing, including steel plants and its ambient residential areas, recreational parks and farmlands. Rock magnetic properties and heavy metal contents were studied. The results show that the magnetic properties of leaf samples are predominated by low-coercivity magnetite, and both the concentration and grain size of magnetite particles gradually decreased with the distance from the main pollution source increases. Moreover, there is a significant linear relationship between magnetic parameters (the low-field magnetic susceptibility, saturation isothermal remanent magnetization and anhysteretic remanent magnetization) and heavy metals contents (Fe, Pb, V, Cr and Zn) (0.73≤R≤ 0.88). Hence, the magnetic parameters of leaves can serve as a proxy for quick detecting of the recent atmospheric metallic pollution.

关键词：首钢集团大气重金属污染磁场参数环境保护

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Recent advances on electroactive CNT-based membranes for environmental applications:The perfect match of electrochemistry and membrane separation

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Chinese Chemical Letters 2020年第10期31卷 2539-2548页

作者： Yanbiao Liu Fuqiang Liu Ning Ding xuemei Hu Chensi shen Fang Li Manhong Huang Zhiwei Wang Wolfgang Sand Chong-Chen Wang Textile Pollution Controlling Engineering Center of Ministry of Environmental Protection College of Environmental Science and EngineeringDonghua UniversityShanghai 201620China Shanghai Institute of Pollution Control and Ecological Security Shanghai 200092China Key Laboratory of Cleaner Production and Integrated Resource Utilization of China National Light Industry Beijing Technology and Business UniversityBeijing 100048China State Key Laboratory of Pollution Control and Resource Reuse School of Environmental Science and EngineeringTongji UniversityShanghai 200092China Institute of Biosciences Freiberg University of Mining and TechnologyFreiberg 09599Germany Beijing Key Laboratory of Functional Materials for Building Structure and Environment Remediation Beijing University of Civil Engineering and ArchitectureBeijing 100044China

Global climate change,growing population,and environmental pollution underscore the need for a greater focus on providing advanced water treatment *** electrochemical basedprocesses are becoming promising solutions,they still face challenges owing to mass transport and upscaling which hinder the exploitation of this *** design and reactor configuration are key factors for achieving operational *** electroactive membrane has proven to be a breakthrough technology integrating electrochemistry and membrane separation with an enhanced mass transport by *** this review article,we discuss recent progress in environmental applications of electroactive membranes with particular focus on those composed of carbon nanotubes(CNT)due to their intriguing physicochemical prope *** applications in degradation of refractory contaminants,detoxification and sequestration of toxic heavy metal ions,and membrane fouling alleviations are systematically *** then discuss the existing limitations and opportunities for future *** development of advanced electroactive systems depends on interdisciplinary collaborations in the areas of materials,electrochemistry,membrane development,and environmental sciences.

关键词： Electroactive membranes Carbon nanotubes Organic pollutants Heavy mental ions Antifouling

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