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西部民族地区旅游产业韧性与高质量发展耦合协调研究

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生态经济 2024年第4期40卷 144-150,164页

作者：胡北明邱雪梅贵州财经大学工商管理学院贵州贵阳550025

旅游产业韧性与高质量发展是旅游业持续发展的两个重要方面,探究二者关系对于地区旅游产业快速走上高质量发展道路具有重要意义。以西部民族地区7个省区为研究对象,运用熵值法对2012—2022年旅游产业韧性和高质量发展进行测度,通过耦合... 详细信息

旅游产业韧性与高质量发展是旅游业持续发展的两个重要方面,探究二者关系对于地区旅游产业快速走上高质量发展道路具有重要意义。以西部民族地区7个省区为研究对象,运用熵值法对2012—2022年旅游产业韧性和高质量发展进行测度,通过耦合协调度模型、相对发展度以及灰色关联度等方法探究二者关系。结果表明:7省区旅游产业韧性与高质量发展水平总体呈现上升态势;耦合协调度呈现“西北低,西南高”的空间分异格局;耦合协调状态皆出现不同程度的提升、下降与回升,且在研究末期更多地表现为产业韧性受阻以及产业高质量发展滞后;旅游产业韧性与产业高质量发展两个系统各级指标的灰色关联系数都大于0.35,处于较好关联状态,其互动关系较为明显。论文的理论探讨和实证分析对旅游产业韧性与旅游高质量发展共进治理格局构建具有一定的启示意义。

关键词：旅游产业韧性旅游高质量发展耦合协调度相对发展度灰色关联度

SOX2-dependent expression of dihydroorotate dehydrogenase regulates oral squamous cell carcinoma cell proliferation

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International Journal of Oral Science 2021年第1期13卷 62-70页

作者： xuemei qiu Sheng Jiang Yanxuan Xiao Yumin He Tao Ren Lu Jiang Rui Liu Qianming Chen State Key Laboratory of Oral Diseases&National Clinical Research Center for Oral Diseases&Chinese Academy of Medical Sciences Research Unit of Oral Carcinogenesis and Management West China Hospital of StomatologySichuan UniversityChengduChina Ministry of science and technology The Second Affiliated Hospital of Chengdu Medical College(China National Nuclear Corporation 416 Hospital)ChengduChina Oncology Department Clinical Medical College and The First Affiliated Hospital of Chengdu Medical CollegeChengduChina

Oral squamous cell carcinoma(OSCC)become a heavy burden of public health,with approximately 300000 newly diagnosed cases and 145000 deaths worldwide per year.Nucleotide metabolism fuel DNA replication and RNA synthesis,which is indispensable for cell proliferation.But how tumor cells orchestrate nucleotide metabolic enzymes to support their rapid growth is largely unknown.Here we show that expression of pyrimidine metabolic enzyme dihydroorotate dehydrogenase(DHODH)is upregulated in OSCC tissues,compared to non-cancerous adjacent tissues.Enhanced expression of DHODH is correlated with a shortened patient survival time.Inhibition of DHODH by either shRNA or selective inhibitors impairs proliferation of OSCC cells and growth of tumor xenograft.Further,loss of functional DHODH imped de novo pyrimidine synthesis,and disrupt mitochondrial respiration probably through destabilizing the MICOS complex.Mechanistic study shows that transcriptional factor SOX2 plays an important role in the upregulation of DHODH in OSCC.Our findings add to the knowledge of how cancer cells co-opt nucleotide metabolism to support their rapid growth,and thereby highlight DHODH as a potential prognostic and therapeutic target for OSCC treatment.

关键词： SOX2 metabolism squamous

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引导编辑技术的研究进展及应用

畜牧兽医学报 2024年第4期55卷 1345-1355页

作者：邱梅玉张雪梅张宁刘明军新疆畜牧科学院生物技术研究所农业农村部草食家畜遗传育种与繁殖重点实验室新疆动物生物技术重点实验室乌鲁木齐830026

以CRISPR/Cas9为基础的引导编辑系统是新开发出的一种基因编辑技术,可以精确实现12种碱基的互换、插入和缺失,并且不需要产生双链断裂和引入外源供体DNA。本文从基因编辑的发展、引导编辑系统的原理、特点、优化、脱靶效应、在动植物和... 详细信息

以CRISPR/Cas9为基础的引导编辑系统是新开发出的一种基因编辑技术,可以精确实现12种碱基的互换、插入和缺失,并且不需要产生双链断裂和引入外源供体DNA。本文从基因编辑的发展、引导编辑系统的原理、特点、优化、脱靶效应、在动植物和基因治疗研究中的应用、引导编辑系统的设计等几方面进行综述,为促进相关领域的科研工作者了解引导编辑系统及进一步利用引导编辑系统在动植物科学基础研究和育种方面的应用提供指导。

关键词： CRISPR/Cas9 引导编辑(prime editing PE) pegRNA 应用研究

TUBB2A基因新发变异导致癫痫伴发育迟缓及脑发育畸形一个家系的遗传学分析

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中华医学遗传学杂志 2024年第2期41卷 187-192页

作者：赵娟徐那李玉芬杨莉邱世彦朱丽萍孙学梅青岛大学第十一临床医学院临沂市人民医院儿内科临沂276000

目的探究1个癫痫伴发育迟缓及脑发育畸形家系的临床表现及致病基因变异情况。方法选取2022年7月2日于临沂市人民医院儿内科就诊的1个癫痫伴发育迟缓及脑发育异常家系为研究对象。收集患儿及其家系成员的临床资料,应用高通量测序技术对... 详细信息

目的探究1个癫痫伴发育迟缓及脑发育畸形家系的临床表现及致病基因变异情况。方法选取2022年7月2日于临沂市人民医院儿内科就诊的1个癫痫伴发育迟缓及脑发育异常家系为研究对象。收集患儿及其家系成员的临床资料,应用高通量测序技术对患儿及其姐姐与父母进行基因检测,采用Sanger测序法进行验证。结果患儿为6岁男性,自幼全面生长发育迟缓,间断抽搐4年余,癫痫发作存在热敏感特点,头颅影像学提示脑发育畸形,视频脑电图提示异常放电。高通量测序结果显示患儿及其姐姐均携带TUBB2A基因c.5G>T(p.Arg2Leu)杂合变异,其父母为野生型。根据美国医学遗传学与基因组学学会(ACMG)相关标准及指南,该变异评定为致病性变异(PS2+PM2Supporting+PM5+PP1+PP2+PP3)。结论TUBB2A基因c.5G>T(p.Arg2Leu)杂合变异考虑是该家系的遗传学病因,可能存在生殖腺细胞嵌合的情况。

关键词： TUBB2A 癫痫发育迟缓脑发育畸形

基于生物信息学分析探讨circRNA在口腔鳞状细胞癌中的调控作用

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福建医科大学学报 2024年第1期58卷 26-31,36页

作者：邱在玲林雪梅吕红兵福建省级机关医院口腔中心福州350003 福建省高校口腔医学重点实验室福建医科大学附属口腔医院福州350004

目的通过生物信息学方法和qRT-PCR实验筛选并验证口腔鳞状细胞癌(OSCC)中差异表达的环状RNA(circRNA),并构建OSCC中可能的circRNA-miRNA-mRNA调控网络。方法选取GEO数据库中2个OSCC的circRNA高通量数据,使用R软件识别OSCC中的差异circR... 详细信息

目的通过生物信息学方法和qRT-PCR实验筛选并验证口腔鳞状细胞癌(OSCC)中差异表达的环状RNA(circRNA),并构建OSCC中可能的circRNA-miRNA-mRNA调控网络。方法选取GEO数据库中2个OSCC的circRNA高通量数据,使用R软件识别OSCC中的差异circRNA,对筛选出的circRNA取交集后获得候选circRNA。通过qRT-PCR在人口腔黏膜角质细胞系(HOK)和OSCC细胞系中验证候选circRNA的表达水平。使用circInteractome和circBank数据库预测circRNA下游的miRNA,通过miRDB和TargetScan数据库预测miRNA的靶基因。使用DAVID在线数据库对靶基因进行GO功能和KEGG通路富集分析。通过STRING数据库和Cytoscape软件确定核心基因,采用GEPIA生存分析网站分析其与预后的关系。结果共筛选出3个差异circRNA、7个miRNA和2575个潜在靶mRNA。GO和KEGG富集分析表明,靶基因主要富集在肿瘤相关的通路和生物学过程。基于富集分析、蛋白质相互作用网络图构建和核心基因的生存分析结果,筛选出3个核心基因(EGF、STAT 5 A和LEF 1),从而建立OSCC相关的circRNA-miRNA-核心基因调控网络。结论本研究为circRNA在OSCC中的调控机制提供参考,为OSCC的诊断及靶向治疗拓宽思路。

关键词：口腔鳞状细胞癌环状RNA miRNA 生物信息学

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多囊卵巢综合征患者程序性细胞死亡因子4表达及睾酮与胚胎质量相关性研究

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现代妇产科进展 2024年第3期33卷 177-181页

作者：仇雪梅赵明蕊解晓丽赵淑芹枣庄市妇幼保健院生殖遗传中心枣庄277100

目的:探讨控制性超促排卵(COH)中多囊卵巢综合征(PCOS)患者卵巢颗粒细胞中程序性细胞死亡因子4(PDCD4)表达及睾酮的临床意义。方法:选取于枣庄市妇幼保健院生殖遗传中心行体外受精-胚胎移植(IVF-ET)助孕的COH周期PCOS患者(28例)和非PCO... 详细信息

目的:探讨控制性超促排卵(COH)中多囊卵巢综合征(PCOS)患者卵巢颗粒细胞中程序性细胞死亡因子4(PDCD4)表达及睾酮的临床意义。方法:选取于枣庄市妇幼保健院生殖遗传中心行体外受精-胚胎移植(IVF-ET)助孕的COH周期PCOS患者(28例)和非PCOS患者(40例),经阴道超声引导下取卵后,收集卵泡液及卵丘周围颗粒细胞,提取RNA,逆转录后应用实时定量PCR检测PDCD4表达情况,分析PDCD4表达及睾酮与胚胎质量的相关性。结果:PCOS组和非PCOS组患者的年龄、不孕年限、体质量指数(BMI)、基础FSH、E_(2)、PRL和睾酮比较,差异均无统计学意义(P>0.05)。两组的正常受精率、卵裂率、优胚率、可用胚率和卵母细胞利用率比较,差异均无统计学意义(P>0.05)。PCOS组的PDCD4表达略高于非PCOS组,但差异无统计学意义(P>0.05)。低可用胚组的PDCD4表达高于正常可用胚组(P=0.04)。高睾酮水平组的正常受精率(P=0.00007)和卵母细胞利用率(P=0.038)低于低睾酮水平组。结论:COH中卵巢颗粒细胞PDCD4表达增高与低可用胚率相关,睾酮水平与受精率和卵母细胞利用率密切相关,睾酮水平升高,受精率和卵母细胞利用率降低。

关键词：多囊卵巢综合征 PDCD4 睾酮胚胎质量卵巢颗粒细胞

The function and mechanism of lactate and lactylation in tumor metabolism and microenvironment

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Genes & Diseases 2023年第5期10卷 2029-2037页

作者： Yan Zhang qiu Peng Jinhua Zheng Yuzhong Yang xuemei Zhang Aiyu Ma Yuxia Qin Zailong Qin Xiang Zheng Department of Pathology Affiliated Hospital of Guilin Medical UniversityGuilinGuangxi 541001China Hunan Key Laboratory of Cancer Metabolism Hunan Cancer Hospital and the Affiliated Cancer Hospital of Xiangya School of MedicineCentral South UniversityChangshaHunan 410013China Laboratory of Genetics and Metabolism Maternal and Child Health Hospital of Guangxi Zhuang Autonomous RegionGuangxi Birth Defects Research and Prevention InstituteNanningGuangxi 530003China

Lactate is an end product of glycolysis.Owing to the lactate shuttle concept introduced in the early 1980s,increasing researchers indicate lactate as a critical energy source for mitochondrial respiration and as a precursor of gluconeogenesis.Lactate also acts as a multifunctional signaling molecule through receptors expressed in various cells,resulting in diverse biological consequences including decreased lipolysis,immune regulation,and anti-inflammation wound healing,and enhanced exercise performance in association with the gut microbiome.Furthermore,increasing evidence reveals that lactate contributes to epigenetic gene regulation by lactylating lysine residues of histones,which accounts for its key role in immune modulation and maintenance of homeostasis.Here,we summarize the function and mechanism of lactate and lactylation in tumor metabolism and microenvironment.

关键词： Epigenetic regulation Lactate Lactylation Microenvironment Tumorigenesis

Life History Recorded in the Vagino-cervical Microbiome Along with Multi-omes

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Genomics, Proteomics & Bioinformatics 2022年第2期20卷 304-321页

作者： Zhuye Jie Chen Chen Lilan Hao Fei Li Liju Song Xiaowei Zhang Jie Zhu Liu Tian Xin Tong Kaiye Cai Zhe Zhang Yanmei Ju Xinlei Yu Ying Li Hongcheng Zhou Haorong Lu xuemei qiu Qiang Li Yunli Liao Dongsheng Zhou Heng Lian Yong Zuo Xiaomin Chen Weiqiao Rao Yan Ren Yuan Wang Jin Zi Rong Wang Na Liu Jinghua Wu Wei Zhang Xiao Liu Yang Zong Weibin Liu Liang Xiao Yong Hou Xun Xu Huanming Yang Jian Wang Karsten Kristiansen Huijue Jia BGI-Shenzhen Shenzhen 518083China Shenzhen Key Laboratory of Human Commensal Microorganisms and Health Research BGI-ShenzhenShenzhen 518083China Department of Biology University of CopenhagenCopenhagen DK-2100Denmark Shenzhen Engineering Laboratory of Detection and Intervention of Human Intestinal Microbiome BGI-ShenzhenShenzhen 518083China BGI Education Center University of Chinese Academy of SciencesShenzhen 518083China China National Genebank BGI-ShenzhenShenzhen 518120China BGI-Qingdao BGI-ShenzhenQingdao 266555China James D.Watson Institute of Genome Sciences Hangzhou 310058China

The vagina contains at least a billion microbial cells,dominated by lactobacilli.Here we perform metagenomic shotgun sequencing on cervical and fecal samples from a cohort of 516 Chinese women of reproductive age,as well as cervical,fecal,and salivary samples from a second cohort of 632 women.Factors such as pregnancy history,delivery history,cesarean section,and breastfeeding were all more important than menstrual cycle in shaping the microbiome,and such information would be necessary before trying to interpret differences between vagino-cervical microbiome data.Greater proportion of Bifidobacterium breve was seen with older age at sexual debut.The relative abundance of lactobacilli especially Lactobacillus crispatus was negatively associated with pregnancy history.Potential markers for lack of menstrual regularity,heavy flow,dysmenorrhea,and contraceptives were also identified.Lactobacilli were rare during breastfeeding or post-menopause.Other features such as mood fluctuations and facial speckles could potentially be predicted from the vagino-cervical microbiome.Gut and salivary microbiomes,plasma vitamins,metals,amino acids,and hormones showed associations with the vagino-cervical microbiome.Our results offer an unprecedented glimpse into the microbiota of the female reproductive tract and call for international collaborations to better understand its long-term health impact other than in the settings of infection or pre-term birth.

关键词： Vagino-cervical microbiome Metagenomic shotgun sequencing Pregnancy history Delivery history Breastfeeding

A metal ions-mediated natural small molecules carrier-free injectable hydrogel achieving laser-mediated photo-Fenton-like anticancer therapy by synergy apoptosis/cuproptosis/anti-inflammation

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Bioactive Materials 2023年第11期29卷 98-115页

作者： Wenmin Pi Linying Wu Jihui Lu Xiaoyu Lin xuemei Huang Zhijia Wang Zhihua Yuan Hailing qiu Jianglan Zhang Haimin Lei Penglong Wang School of Chinese Pharmacy Beijing University of Chinese MedicineBeijing102488China School of Traditional Chinese Medicine Beijing University of Chinese MedicineBeijing102488China

Tumor microenvironment (TME) plays an important role in the tumorigenesis, proliferation, invasion and metastasis. Thereby developing synergistic anticancer strategies with multiple mechanisms are urgent. Copper is widely used in the treatment of tumor chemodynamic therapy (CDT) due to its excellent laser-mediated photo- Fenton-like reaction. Additionally, copper can induce cell death through cuproptosis, which is a new modality different from the known death mechanisms and has great promise in tumor treatment. Herein, we report a natural small molecules carrier-free injectable hydrogel (NCTD Gel) consisted of Cu2+-mediated self-assembled glycyrrhizic acid (GA) and norcantharidin (NCTD), which are mainly governed by coordination and hydrogen bonds. Under 808 nm laser irradiation, NCTD Gel can produce reactive oxygen species (ROS), consume glutathione (GSH) and overcome hypoxia in TME, leading to synergistically regulate TME via apoptosis, cuproptosis and anti-inflammation. In addition, NCTD Gel’s CDT display high selectivity and good biocompatibility as it relies on the weak acidity and H2O2 overexpression of TME. Notably, NCTD Gel’s components are originated from clinical agents and its preparation process is easy, green and economical, without any excipients. This study provides a new carrier-free hydrogel synergistic antitumor strategy, which has a good prospect in industrial production and clinical transformation.

关键词： Natural small molecular ternary carrier-free hydrogel Tumor microenvironment Cuproptosis Laser-mediated photo-fenton-like reaction