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头针联合集体语言训练治疗儿童语言发育迟缓临床研究

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新中医 2024年第1期56卷 155-159页

作者：刘琴胡玮吕琳璐傅雪梅陈莉娜永康市妇幼保健院儿童康复科浙江永康321300

目的:观察头针联合集体语言训练治疗儿童语言发育迟缓的临床疗效。方法:采用随机数字表法将60例语言发育迟缓患儿分为治疗组与对照组各30例。对照组采取集体语言训练,治疗组在对照组基础上给予头针治疗。比较2组临床疗效及治疗前后中医... 详细信息

目的:观察头针联合集体语言训练治疗儿童语言发育迟缓的临床疗效。方法:采用随机数字表法将60例语言发育迟缓患儿分为治疗组与对照组各30例。对照组采取集体语言训练,治疗组在对照组基础上给予头针治疗。比较2组临床疗效及治疗前后中医证候积分、盖泽尔发育量表(GDS)、语言发育迟缓检查法(S-S)评分、功能性语言沟通能力量表(CFCP)评分。结果:治疗后,治疗组总有效率96.67%,高于对照组80.00%(P<0.05)。治疗后,2组GDS维度(语言、适应性、精细动作、大运动及个人-社交能力)评分均较治疗前升高(P<0.05),治疗组治疗后GDS维度评分均高于对照组(P<0.05)。治疗后,2组中医证候积分均较治疗前降低(P<0.05),S-S评分、CFCP评分升高(P<0.05);治疗组治疗后中医证候积分低于对照组(P<0.05),S-S评分、CFCP评分高于对照组(P<0.05)。结论:头针联合集体语言训练治疗儿童语言发育迟缓疗效显著,能有效缓解患儿临床症状,改善语言功能,提高语言沟通能力。

关键词：语言发育迟缓儿童头针集体语言训练语言沟通能力

The immunogenicity of cells derived from induced pluripotent stem cells

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Cellular & Molecular Immunology 2014年第1期11卷 14-16页

作者： xuemei fu Shenzhen Children's Hospital Shenzhen China

With their ability to undergo unlimited self-renewal in culture and to differentiate into all cell types in the body, human embryonic stem ceils （hESCs） hold great potential for the treatment of currently incurable diseases. Two hESC-based cell therapies for spinal cord injury and macular degeneration have been advanced into human clinical trials. Despite this rapid progress, one key challenge of hESC-based cell therapy is the allogeneic immune rejection of hESC-derived cells by recipients. This problem could be mitigated by a recent breakthrough in the technology of induced pluripotent stem cells （iPSCs） by nuclear reprogramming of patient-specific somatic cells with defined factors, which could become a renewable source of autologous ceils for cell therapy. However, recent studies revealing the abnormal epigenetics, genomic stability and immunogenicity of iPSCs have raised safety concerns over iPSC-based therapy. Recent findings related to the immunogenicity of iPSC derivatives will be summarized in this review.

关键词： immune tolerance immunogenicity induced pluripotent stem cells minor antigen transplantation

Using a novel cellular platform to optimize CRISPR/CAS9 technology for the gene therapy of AIDS

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Protein & Cell 2017年第11期8卷 848-852页

作者： Jingjin He Thanutra Zhang xuemei fu The Eighth Affiliated Hospital of Sun Yat-sen University Shenzhen 518033 China Division of Biological Sciences University of California San Diego 9500 Gilman Drive La Jolla CA 92093 USA Shenzhen Children's Hospital Shenzhen 518026 China

Dear Editor,Despite tremendous effort devoted to the development of antiretroviral therapies to combat HIV over the past decades, AIDS remains one of the most important global infectious diseases. According to UNAIDS report on the global AIDS epidemic in 2016, the estimated number of people living with HIV rose from 7.5 million in 2010 to 36.7 million in 2015. furthermore, drug-resistance HIV strains have recently been reported （Wensing et al., 2017）. Therefore, it is important to develop new therapies to eliminate HIV in the patients. Immortalized cell lines representing the major targets of HIV in human are important for HIV research and therapeutic development.

关键词： novel cellular platform optimize CRISPR/CAS9 technology the gene therapy of AIDS

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成都市高校大学生无偿献血认知水平调查

国际输血及血液学杂志 2024年第1期47卷 54-60页

作者：李莹胡萍方文攀刘璇赵宗瀚肖湘周敏席光湘傅雪梅成都市血液中心成都610020

目的调查成都市高校大学生群体对无偿献血的认知水平。方法2020年11月至2021年6月,通过高校献血车进校园邀请大学生填写电子问卷的方式,选择成都市区内10所高校的1265例在校大学生为研究对象。学生年龄为18~21岁;男、女性为分别为548和... 详细信息

目的调查成都市高校大学生群体对无偿献血的认知水平。方法2020年11月至2021年6月,通过高校献血车进校园邀请大学生填写电子问卷的方式,选择成都市区内10所高校的1265例在校大学生为研究对象。学生年龄为18~21岁;男、女性为分别为548和717例。根据研究对象是否参加过无偿献血,将其分为研究组(n=425,参与过无偿献血)和对照组(n=840,未参与过无偿献血)。2组学生年龄比较,差异无统计学意义(P>0.05)。本研究采用自行设计的调查问卷,对大学生的基本情况,无偿献血知识知晓情况,无偿献血动机、感受和意愿5个方面进行调查。2组大学生的无偿献血知识、感受得分等比较,采用独立样本t检验;2组大学生性别、籍贯构成比等比较,采用χ^(2)检验。本研究遵循的程序符合2013年修订版《世界医学协会赫尔辛基宣言》。结果①本研究1265例大学生中,以女性(56.7%,717/1265),农村户籍(69.6%,881/1265),本科及以上教育程度(83.2%,1052/1265)及非医药类专业(98.6%,1247/1265)大学生为主。1265例大学生的献血率为33.6%(425/1265)。研究组与对照组大学生的性别、专业、健康自评情况及家庭年平均收入构成比分别比较,差异均有统计学意义(χ^(2)=87.55,P<0.001;χ^(2)=8.95,P=0.003;χ^(2)=16.92,P=0.002;χ^(2)=11.18,P=0.011)。②本研究1265例大学生的无偿献血知识总知晓率为75.0%(949/1265);研究组大学生的无偿献血知识得分为(11.6±2.1)分,高于对照组的(11.1±2.4)分,并且差异有统计学意义(t=3.57,P<0.001)。③研究组大学生的无偿献血感受得分为(34.2±9.4)分,高于对照组的(29.1±8.1)分,并且差异有统计学意义(t=9.98,P<0.001)。④研究组425例大学生参加无偿献血的主要原因为奉献爱心(85.6%,364/425)和检查血型(53.6%,228/425);对照组840例大学生未参加无偿献血的主要原因为时间不合适(53.2%,447/840)和认为自身不符合献血要求(50.1%,421/840)。⑤本研究1265例大学生中,愿意参加无偿献血的人数占87.4%(1106/1265)。研究组和对照组大学生的无偿献血意愿构成比比较,差异有统计学意义(χ^(2)=6.30,P=0.043);并且研究组大学生愿意参加献血的比例高于对照组[90.4%(384/425)比86.0%(722/840);χ^(2)=4.97,P=0.026]。结论成都市高校大学生无偿献血知识知晓率较好,献血意愿较强,但献血率处于较低水平。血站无偿献血工作中,应加强对高校大学生的无偿献血知识宣讲、消除大学生无偿献血顾虑、优化献血服务时间等,从而招募更多大学生参与无偿献血,为未来的血液供应提供更持续的保障。

关键词：无偿献血大学生认知动机意愿

functional analysis of the acetylation of human p53 in DNA damage responses

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Protein & Cell 2014年第7期5卷 544-551页

作者： Sun-Ku Chung Shengyun Zhu Yang Xu xuemei fu Shenzhen Children's Hospital 7019 Yitian Road Shenzhen Guangdong 518026 China Division of Biological Sciences University of California San Diego 9500 Gilman Drive La Jolla CA 92093 USA Chongqing Medical University Chongqing 400016 China

As a critical tumor suppressor, p53 is inactivated in human cancer cells by somatic gene mutation or disruption of pathways required for its activation. Therefore, it is critical to elucidate the mechanism underlying p53 activation after genotoxic and cellular stresses. Accumulating evidence has indicated the importance of posttranslational modifications such as acetylation in regulating p53 stability and activity. However, the physiological roles of the eight identified acetylation events in regulating p53 responses remain to be fully understood. By employing homologous recombination, we introduced various combinations of missense mutations （lysine to arginine） into eight acetylation sites of the endogenous p53 gene in human embryonic stem cells （hESCs）. By determining the p53 responses to DNA damage in the p53 knock-in mutant hESCs and their derivatives, we demonstrate physiological importance of the acetylation events within the core domain （Kt20 and K164） and at the C-terminus （K370/372/373/381/382/ 386） in regulating human p53 responses to DNA damage.

关键词： human embryonic stem cells (hESCs) p53 acetylation homologous recombination DNA damage cancer

functions of p53 in pluripotent stem cells

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Protein & Cell 2020年第1期11卷 71-78页

作者： xuemei fu Shouhai Wu Bo Li Yang Xu Jingfeng Liu The Eighth Affiliated Hospital Sun Yat-sen UniversityShenzhen 518033China Center for Regenerative and Translational Medicine Guangdong Provincial Academy of Chinese Medical SciencesThe Second Affiliated Hospital of Guangzhou University of Chinese MedicineGuangzhou 510632China Department of Biochemistry and Molecular Biology Zhongshan School of MedicineSun Yat-sen UniversityGuangzhou 510080China Cancer Research Institute Guangdong Provincial Key Laboratory of Tumor ImmunotherapySchool of Basic Medical SciencesSouthern Medical UniversityGuangzhou 510515China

Pluripotent stem cells(PSCs)are capable of unlimited self-renewal in culture and differentiation into all functional cell types in the body,and thus hold great promise for regenerative *** achieve their clinical potential,it is critical for PSCs to maintain genomic stability during the extended *** critical tumor suppressor p53 is required to maintain genomic stability of mammalian *** response to DNA damage or oncogenic stress,p53 plays multiple roles in maintaining genomic stability of somatic cells by inducing cell cycle arrest,apoptosis,and senescence to prevent the passage of genetic mutations to the daughter cells.p53 is also required to maintain the genomic stability of ***,in response to the genotoxic stresses,a primary role of p53 in PSCs is to induce the differentiation of PSCs and inhibit pluripotency,providing mechanisms to maintain the genomic stability of the self-renewing *** addition,the roles of p53 in cellular metabolism might also contribute to genomic stability of PSCs by limiting oxidative *** summary,the elucidation of the roles of p53 in PSCs will be a prerequisite for developing safe PSC-based cell therapy.

关键词： p53 embryonic stem cells induced pluripotent stem cells genetic stability metabolism

Homologous recombination in human embryonic stem cells using CRISPR/Cas9 nickase and a long DNA donor template

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Protein & Cell 2014年第4期5卷 258-260页

作者： Zhili Rong Shengyun Zhu Yang Xu xuemei fu Division of Biological Sciences University of CaliforniaSan Diego9500 Gilman DriveLa JollaCA 92093USA Shenzhen Children’s Hospital 7019 Yitian RoadShenzhen 518026China Chongqing Medical University Chongqing 400016China

Dear Editor Genome editing of human embryonic stem cells （hESCs） is critical for basic biological research and regenerative medi- cine. However, until a few years ago, gene targeting tech- nologies to disrupt, repair or overexpress genes in hESCs had been very inefficient and thus could not be routinely used. Recent technical breakthrough includes bacterial arti- ficial chromosome based high efficiency gene targeting （Song et al., 2010）, as well as the successful engineering of two systems of site-specific nucleases, zinc-finger nucleases （ZFNs） and transcription activator-like effector nucleases （TALENs）（Hockemeyer et al., 2009; Hockemeyer et al.,

关键词： Bacterial Artificial Chromosome Human Embryonic Stem Cell Cluster Regularly Interspaced Short Palindromic Repeat Random Integration Cas9 Nuclease

Synthesis and structure of Au_(19)Ag_4(S-Adm)_(15) nanocluster:Polymorphs and optical properties

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Chinese Chemical Letters 2021年第1期32卷 565-568页

作者： xuemei fu Xinzhang Lin Xiuqing Ren Hengjiang Cong Chao Liu Jiahui Huang Gold Catalysis Research Center State Key Laboratory of CatalysisDalian Institute of Chemical PhysicsChinese Academy of SciencesDalian 116023China University of Chinese Academy of Sciences Beijing 100049China College of Chemistry and Molecular Sciences Engineering Research Center of Organosilicon Compounds&MaterialsMinistry of EducationWuhan UniversityWuhan 430072China

Polymorphism is a common phenomenon in ***,we report one-pot wet chemical method to synthesize two polymorphs of Au_(19)Ag_(4)(S-Adm)_(15) nanocluster protected by 1-adamantanethiol(HSAdm),which adopt P-1 and P2_(1)/c space group *** crystal structures of two polymorphs were determined by X-ray *** to the previously reported Au_(19)Ag_(4)(S-Adm)_(15)nanocluster adopting P2_(1)/n space group,polymorphs of Au_(19)Ag_(4)(S-Adm)_(15) with P-1 and P2_(1)/c space group show the different optical *** r,Au_(19)Ag_(4)(S-Adm)_(15) with P-1 space group exhibits good thermal ***,we investigated the effect of solvent and molar ratio of metal precursors on the *** work provides an insight to polymorphs of metal nanoclusters.

关键词： Au_(19)Ag_4(S-Adm)_(15) Alloy nanocluster Polymorph Fluorescence Thermal stability

Highly Efficient Synthesis of Au130（SPh-Br）50 Nanocluster

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Chemical Research in Chinese Universities 2018年第5期34卷 719-722页

作者： REN Xiuqing fu xuemei LIN Xinzhang LIU Chao HUANG Jiahui YAN Jinghui School of Chemistry & Environmental Engineering Changchun University of Science and Technology Changchun 130022 P. R. China Gold Catalysis Research Center State Key Laboratory of Catalysis Dalian Institute of Chemical Physics Chinese Academy of Sciences Dalian 116023P. R. China University of Chinese Academy of Sciences Beijing 100049 R R. China

We reported the synthesis ofAul3o（SPh-Br）50（Br-Ph-SH=4-bromothiophenol） nanocluster with high purity and high yield via ＂size focusing＂ and ＂ligand exchange＂ processes. The time of synthetic process was significantly reduced compared with previous synthetic routine. AU130（SPh-Br）50 was determined by UV-Vis absorption spectros- copy and matrix-assisted laser desorption ionization（MALDI） mass spectroscopy. Thermo-gravimetric analysis （TGA） and size-exclusion chromatogram（SEC） analyses confirmed the purity of AU130（SPh-Br）50. The yield of gold nanoc- lusters was 20%（based on HAuCl4）.

关键词： Au130 Gold nanocluster Thiolate ligand