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Astragaloside IV derivative HHQ16 ameliorates infarctioninduced hypertrophy and heart failure through degradation of lncRNA4012/9456

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Signal Transduction and Targeted Therapy 2023年第11期8卷 5454-5469页

作者： Jingjing Wan Zhen zhang hennan Wu Saisai Tianbei Zang Ge Jin Qingyan Sun Pin Wang Xin Luan Yili Yang xuelin zhan Lingyu Linda Ye Dayue Darrel Duan Xia Liu Weidong zhang School of Pharmacy Second Miltary Medical UniversityShanghaiPR China China Institute of Pharmaceutical Industry ShanghaiPR China Key Laboratory of Medical Immunology and Institute of Immunology Second Military Medical UniversityShanghaiPR China Institute of Interdisciplinary Integrative Medicine Research Shanghai University of Traditional Chinese MedicineShanghaiPR China China Regional Research Centre International Centre of Genetic Engineering&BiotechnologyTaizhouPR China State Key Laboratory of Medicinal Chemical Biology College of PharmacyNankai UniversityTianjinPR China Center for Phenomics of Traditional Chinese Medicine Hospital of Traditional Chinese Medicine Affliated to Southwest Medical UniversitySouthwest Medical UniversityLuzhouPR China Key Laboratory of Autoimmune Diseases and Precision Medicine People's Hospital of Ningxia Hui Autonomous RegionYinchuanPR China Institute of Medicinal Plant Development Chinese Academy of Medical Sciences and Peking Union Medical CollegeBeijingPR China

Reversing ventricular remodeling represents a promising treatment for the post-myocardial infarction(MI)heart failure(HF).Here,we report a novel small molecule HHQ16,an optimized derivative of astragaloside IV,which effectively reversed infarction-induced myocardial remodeling and improved cardiac function by directly acting on the cardiomyocyte to reverse *** effect of HHQ16 was associated with a strong inhibition of a newly discovered Egr2-affliated transcript Inc9456 in the *** minimally expressed in normal mouse heart,Inc9456 was dramatically upregulated in the heart subjected to left anterior descending coronary artery ligation(LADL)and in cardiomyocytes subjected to hypertrophic *** critical role of Inc9456 in cardiomyocyte hypertrophy was confirmed by specific overexpression and knockout in vitro.A physical interaction between Inc9456 and G3BP2 increased NF-kB nuclear translocation,triggering hypertrophy-related ***16 physically bound to Inc9456 with a high-affinity and induced its ***-specific Inc9456 overexpression induced,but knockout prevented LADL-induced,cardiac hypertrophy and ***16 reversed the effect of Inc9456 overexpression while lost its protective role when Inc9456 was deleted,further confirming Inc9456 as the bona fide target of *** further identified the human ortholog of Inc9456,also an Egr2-affliated transcript,***,Inc4012 was significantly upregulated in hypertrophied failing hearts of patients with dilated ***16 also specifically bound to Inc4012 and caused its degradation and antagonized its hypertrophic *** degradation of pathological increased Inc4012/lnc9456 by small molecules might serve as a novel promising strategy to regress infarction-induced cardiac hypertrophy and HF.

关键词： hypertrophy infarction protective

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红芽芋低温疗法脱毒苗遗传变异的AFLP检测

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分子植物育种 2018年第14期16卷 4685-4695页

作者：柯维忠徐文慧谢妮妮吴夏俊鹏占学林上饶师范学院生命科学学院上饶334001

为探明红芽芋低温疗法脱毒苗的遗传变异,本研究利用AFLP荧光标记和毛细管电泳分离技术对其进行分析。研究表明,利用10对引物组合对江西铅山红芽芋4种低温疗法脱毒苗和大田苗30个样本进行扩增,扩增后的多态性位点数在49~70范围之内。多... 详细信息

为探明红芽芋低温疗法脱毒苗的遗传变异,本研究利用AFLP荧光标记和毛细管电泳分离技术对其进行分析。研究表明,利用10对引物组合对江西铅山红芽芋4种低温疗法脱毒苗和大田苗30个样本进行扩增,扩增后的多态性位点数在49~70范围之内。多态性百分率最低是E36M72,为89.23%,多态性百分率最高是E59M66,为100%;观测等位基因数Na在1.892 3~2.000 0的范围之内,有效等位基因数Ne在1.616 6~1.908 2的范围之内;Nie's基因多样度H的范围为0.357 4~0.473 8,Shannon信息指数I的范围为0.526 8~0.666 2;依据其荧光AFLP扩增结果进行编码组合,构建了江西铅山红芽芋AFLP组合编码指纹图谱。4种低温疗法脱毒苗和大田苗30个样本均出现了AFLP变异位点,每个样本变异位点平均出现的数量为8.73~23.30;4种低温疗法脱毒苗和大田苗30个样本的遗传相似系数为0.471 8~0.796 2,样本间的相似度不是很高,低温疗法脱毒存在一定程度上的变异。在遗传相似系数0.605 1处,4种低温疗法脱毒苗和大田苗30个样本被分为4大类。第Ⅰ类包括样本1和样本30,第Ⅱ类包括样本27和样本29,第Ⅲ类包括样本26和样本28,剩下的样本均归于第Ⅳ类。表明低温疗法脱毒造成了江西铅山红芽芋一定程度的遗传变异。本试验结果为江西铅山红芽芋茎尖低温疗法脱毒提供一定的理论依据。

关键词：江西铅山红芽芋低温疗法脱毒苗遗传变异 AFLP检测

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矮牵牛花香成分的鉴定及相关基因的表达分析

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分子植物育种 2022年第15期20卷 4931-4939页

作者：郭莹詹雪林周涵奇邱雨刘坤祥徐虹西北农林科技大学生命科学学院杨陵712100

为了研究矮牵牛花香成分与相关基因之间的联系,本研究以杂交选育的3个芳香矮牵牛株系(5,6,17号)为材料,采用静态顶空进样-气相色谱-质谱联用技术(SPME-GC-MS)和实时荧光定量技术,鉴定芳香矮牵牛鲜花的香气成分,分析花香物质代谢相关基... 详细信息

为了研究矮牵牛花香成分与相关基因之间的联系,本研究以杂交选育的3个芳香矮牵牛株系(5,6,17号)为材料,采用静态顶空进样-气相色谱-质谱联用技术(SPME-GC-MS)和实时荧光定量技术,鉴定芳香矮牵牛鲜花的香气成分,分析花香物质代谢相关基因的表达特征。结果表明:3个株系共鉴定出47种花香组分,其中萜类化合物的种类多且含量较高,株系间花香物质组成差异较大;通过比较看出,6号株系萜类化合物的含量较高,17号株系苯基/苯丙类物质的含量较高,5号株系脂肪酸类物质的含量较高;PAAS、BPBT、PAL都是调控苯基/苯丙类化合物合成的关键酶,EOBⅡ是苯丙烷类挥发性生物合成调控因子,在开花期5号株系中PAAS与PAL基因表达上调,6号株系中的BPBT基因表达上调,17号株系中的PAAS、BPBT和EOBⅡ基因表达显著上调,这些基因与香气组分中苯基/苯丙类物质的含量密切相关。研究结果可为后续培育芳香矮牵牛新品系、克隆矮牵牛花香基因、研究利用矮牵牛特有花香物质提供参考依据。

关键词：矮牵牛(Petunia hybrida) 花香物质 SPME-GC-MS 基因表达

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黄独胚性愈伤组织冻后黑暗培养及其再生植株半薄切片观察

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浙江农业学报 2018年第1期30卷 65-70页

作者：洪森荣宁本松叶思雨刘燕张铭心占学林上饶师范学院生命科学学院江西上饶334001

以黄独胚性愈伤组织为材料,对黄独胚性愈伤组织冻后黑暗培养的时间进行探讨,并对冻后黑暗培养胚性愈伤组织的显微结构及其再生植株进行半薄切片观察。结果表明,包埋玻璃化法超低温保存后,黑暗培养1~5 d,黄独胚性愈伤组织的冻后成活率随... 详细信息

以黄独胚性愈伤组织为材料,对黄独胚性愈伤组织冻后黑暗培养的时间进行探讨,并对冻后黑暗培养胚性愈伤组织的显微结构及其再生植株进行半薄切片观察。结果表明,包埋玻璃化法超低温保存后,黑暗培养1~5 d,黄独胚性愈伤组织的冻后成活率随着时间的延长而增加,超过5 d,成活率显著下降。半薄切片观察结果表明,冻后黄独胚性愈伤组织直接置于光周期下培养,会造成细胞排列疏松,局部出现较大的细胞空隙;而经黑暗培养后再置于光周期下培养,细胞排列较为紧密,细胞空隙较小。经半薄切片观察,黄独冻后黑暗培养胚性愈伤组织再生植株与常温继代再生植株相比较,根、茎、叶结构无显著差异,叶肉细胞的平均叶绿体数量也无显著差异。因此,冻后黑暗培养一定程度上保证了胚性愈伤组织细胞结构的完整性,且其再生植株无形态学变异。

关键词：黄独胚性愈伤组织超低温保存冻后黑暗培养再生植株半薄切片观察

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三叶青种质资源遗传多样性的随机扩增多态性DNA分析

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浙江农业学报 2018年第11期30卷 1839-1848页

作者：尹明华占学林徐文慧谢妮妮蔡红陈荣华上饶师范学院生命科学学院江西上饶334001 上饶市红日农业开发有限公司江西上饶334700

利用RAPD技术对64个三叶青种质资源样本进行遗传多样性分析,从40对引物中筛选出10对引物进行批量PCR实验。结果表明:64个三叶青样本的观测等位基因数(Na)为1. 666 7～2. 000 0,有效等位基因数(Ne)为1. 247 9～1. 701 3,Nei’s基因多样... 详细信息

利用RAPD技术对64个三叶青种质资源样本进行遗传多样性分析,从40对引物中筛选出10对引物进行批量PCR实验。结果表明:64个三叶青样本的观测等位基因数(Na)为1. 666 7～2. 000 0,有效等位基因数(Ne)为1. 247 9～1. 701 3,Nei’s基因多样性指数为0. 167 0～0. 398 4,Shannon多样性指数(I)为0. 262 8～0. 583 0,平均多态位点为7. 5,平均多态百分数为93. 145%;在遗传相似系数0. 720 56处,可以将64个三叶青样本分成11大类;在遗传相似系数0. 697 04处,则可将64个三叶青样本分成4大类;聚类分析的结果与种源的地理距离存在不一致性,这可能与种质资源库的地形、气候等自然因素有关。表明所测三叶青种质资源遗传多样性丰富,具有一定的开发利用价值,可为合理保护三叶青的基因资源及其遗传改良提供科学依据。

关键词：三叶青种质资源遗传多样性随机扩增多态性DNA

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Diagenesis and Evolution of the Holocene Coquinite from the Haishan Island,Eastern Guangdong,China

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Acta Geologica Sinica(English Edition) 2009年第1期83卷 70-80页

作者： SUN Jinlong XU Huilong QIU xuelin zhan Wenhuan LI Yamin CAS Key Laboratory of Marginal Sea Geology South China Sea Institute of Oceanology the Chinese Academy of Sciences Guangzhou Guangdong 510301 China Graduate University of the Chinese Academy of Sciences Beijing 100049 China

The coastal hard rock with a thickness of over 5 m and a distribution area of nearly 200 ha in the Haishan Island, south China, has long drawn researchers＇ attention. However, there were controversies over its formation and classification, and these controversies in turn lead to the dispute of sea level changes and coastal uplift-subsidence of this area. To investigate its diagenesis and evolution, petrographic analysis, elemental geochemistry, isotopic analysis, and radiocarbon dating were used in the present study. Radiocarbon dating indicates that the deposition of the Haishan Coquinite commenced in the mid Holocene and lasted to -600 a B.P. Petrographic analysis shows that the Haishan Coquinite is cemented by low-Mg calcite, indicating that the cementation occurred in a meteoric environment. The elemental geochemistry and isotopic values demonstrate that the coquinite suffered strong leaching, which was thought to be responsible for the meteoric cementation of the coquinite. According to these results, the diagenesis of the coquinite is revealed： deposition of the Haishan Coquinite commenced in the mid Holocene in a shoal environment, initial cementation occurred and cement may be high-Mg calcite or aragonite; latterly the coquinite exposed to meteoric environment as a result of lowering of relative sea level, and the cement altered to low-Mg, which took the morphologies of bladed calcite rim and equant spar. A four-stage evolution model is proposed：（1） deposition stage, （2） initial cementation, （3） exposure to and cementation in meteoric environment, and （4） erosion stage. The published reports indicate that the hard rock should be designated as coquinite. Based on these studies, mid-Holocene sea level in this area was discussed, and the Haishan Island was proposed to uplift with a rate of ～5 mm/a in the last -600 a.

关键词： beachrock coquinite diagenesis evolution model mid Holocene sea level

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低温离体保存黄独微型块茎转录组、蛋白质组和代谢组的关联分析

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浙江农业学报 2017年第11期29卷 1827-1834页

作者：洪森荣吴夏俊鹏徐文慧占学林谢妮妮蒋妍汪金华凌飞吴丽霞万琳上饶师范学院生命科学学院江西上饶334001

为探究黄独微型块茎低温离体保存的内在机理,对其转录组、蛋白质组和代谢组进行关联分析。结果表明:黄独微型块茎低温和常温离体保存的转录本比例和蛋白比例的log2值均呈现正态分布,十分类似,符合真实生物样品的要求;差异蛋白和差异转... 详细信息

为探究黄独微型块茎低温离体保存的内在机理,对其转录组、蛋白质组和代谢组进行关联分析。结果表明:黄独微型块茎低温和常温离体保存的转录本比例和蛋白比例的log2值均呈现正态分布,十分类似,符合真实生物样品的要求;差异蛋白和差异转录本韦恩图结果表明,84个转录本在两者中均有差异,表明大多数差异的转录本在蛋白组数据均有差异;差异蛋白和差异转录本的log2对数热图分析结果表明,相对于蛋白质组来说,转录组差异程度更大,两者的差异转录本多数一致,少数不同;差异蛋白和差异转录本log2散点关联密度图分析结果表明,两者的比例在0附近居多,左下角和右上角(转录本和蛋白的上下调关系一致)相对左上角和右下角(转录和蛋白上下调关系不一致)更多,表明两者差异表达趋势一致性的程度更高。低温离体保存黄独微型块茎转录组、蛋白质组和代谢组的关联分析结果表明,低温离体保存的黄独微型块茎主要涉及碳代谢、氨基酸生物合成、糖酵解途径、淀粉蔗糖代谢和丙酮酸代谢等途径,这为黄独微型块茎的低温离体保存和低温破除休眠提供了理论依据。

关键词：黄独微型块茎低温离体保存转录组蛋白质组代谢组

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EARTHQUAKE FOCAL MECHANISM AND ITS TECTONIC SIGNIFICANCE ALONG THE TWO SIDES OF THE RED RIVER FAULT ZONE

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Geotectonica et Metallogenia 2004年第1期28卷 79-92页

作者： ZHU Junjiang zhan Wenhuan QIU xuelin XU Huilong TANG Cheng Key Laboratory of Marginal Sea Geology South China Sea Institute of Oceanology & Guangzhou Institute of Geochemistry Chinese Academy of Sciences Guangzhou GD 510301 China Key Laboratory of Marginal Sea Geology South China Sea Institute of Oceanology & Guangzhou Institute of Geochemistry Chinese Academy of Sciences Guangzhou GD 510301 China Key Laboratory of Marginal Sea Geology South China Sea Institute of Oceanology & Guangzhou Institute of Geochemistry Chinese Academy of Sciences Guangzhou GD 510301 China Key Laboratory of Marginal Sea Geology South China Sea Institute of Oceanology & Guangzhou Institute of Geochemistry Chinese Academy of Sciences Guangzhou GD 510301 China Key Laboratory of Marginal Sea Geology South China Sea Institute of Oceanology & Guangzhou Institute of Geochemistry Chinese Academy of Sciences Guangzhou GD 510301 China

The Red River Fault Zone is a gigantic slide-slip fault zone extending up to 1000km from Tibet to SouthChina Sea. It has been divided into the north, central and south segments according to the difference of thegeometry, kinetics, and seismicity on the land, but according to the contacted relationship between the old pre-Cenozoic block in Indochina Peninsula and the South China block, the Red River Fault Zone was divided into two parts extending from land to ocean, the north and south segments. Since the Tertiary, the Red River Fault Zone suffered first the sinistral movement and then the dextral movement. The activities of the north and the south segments were different. Based on the analysis of earthquakes and focal mechanism solutions,earthquakes with the focus depths of 0-33km are distributed over the whole region and more deep earthquakes are distributed on the northeastern sides of the Red River fault. Types of faulting activities are the thrust in the northwest, the normal in the north and the strike-slip in the south, with the odd type, viz. the transition type, in the other region. These show the Red River Fault Zone and its adjacent region suffered the extruding force in NNW direction and the normal stress in NEE direction and it makes the fault in the region extrude-thrust,horizontal strike-slip and extensional normal movement.

关键词： The Red River Fault Zone (RRFZ) Focal mechanism solutions Dextral movement Seismicactivity

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HPDL deficiency causes a neuromuscular disease by impairing the mitochondrial respiration

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Journal of Genetics and Genomics 2021年第8期48卷 727-736页

作者： Yu Sun Xiujuan Wei Fang Fang Yiping Shen Haiyan Wei Jiuwei Li Xianglai Ye Yongkun zhan Xiantao Ye Xiaomin Liu Wei Yang Yuhua Li Xiangju Geng xuelin Huang Yiyan Ruan Zailong Qin Shang Yi Jianxin Lyu Hezhi Fang Yongguo Yu Department of Pediatric Endocrinology and Genetics Xinhua HospitalShanghai Jiao Tong University School of MedicineShanghai Institute for Pediatric ResearchShanghai 200092China Key Laboratory of Laboratory Medicine Ministry of EducationZhejiang Provincial Key Laboratory of Medical GeneticsSchool of Laboratory Medicine and Life SciencesWenzhou Medical UniversityWenzhou 325035China Department of Neurology Beijing Children's HospitalCapital Medical UniversityNational Center for Children's HealthBeijing 100045China The Maternal and Child Health Care Hospital of Guangxi Zhuang Autonomous Region Guangxi Birth Defects Prevention and Control InstituteNanning 530000China Shanghai Children's Medical Center Shanghai Jiao Tong University School of MedicineShanghai 200127China Boston Children's Hospital Harvard Medical SchoolBostonMA 02115USA Department of Endocrinologic and Inherited Metabolic Henan Childen's HospitalZhengzhou Children's HospitalZhengzhou 450078China Department of Radiology Xinhua HospitalShanghai Jiao Tong University School of MedicineShanghai 200092China Department of Rehabilitation Henan Children's HospitalZhengzhou Children's HospitalZhengzhou 450018China Zhejiang Provincial People's Hospital Affiliated People's Hospital of Hangzhou Medical CollegeHangzhou 310014China Shanghai Key Laboratory of Pediatric Gastroenterology and Nutrition Shanghai 200092China

Mitochondrial diseases are caused by variants in both mitochondrial and nuclear genomes.A nuclear gene HPDL(4-hydroxyphenylpyruvate dioxygenase-like),which encodes an intermembrane mitochondrial protein,has been recently implicated in causing a neurodegenerative disease characterized by pediatric-onset spastic movement ***,we report six Chinese patients with bi-allelic HPDL pathogenic variants from four unrelated families showing neuropathic symptoms of variable severity,including developmental delay/intellectual disability,spasm,and *** different pathogenic variants are identified,of which five are *** fibroblasts and immortalized lymphocytes derived from patients show impaired mitochondrial respiratory function,which is also observed in HPDL-knockdown(KD)He La *** these He La cells,overexpression of a wild-type HPDL gene can rescue the respiratory phenotype of oxygen consumption *** addition,a decreased activity of the oxidative phosphorylation(OXPHOS)complex II is observed in patient-derived lymphocytes and HPDL-KD He La cells,further supporting an essential role of HPDL in the mitochondrial respiratory ***,our data expand the clinical and mutational spectra of this mitochondrial neuropathy and further delineate the possible disease mechanism involving the impairment of the OXPHOS complex II activity due to the bi-allelic inactivations of HPDL.

关键词： HPDL gene Mitochondrial disease Respiration impairment OXPHOS Respiration chain complexⅡ

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Review of the Status and Trend on HEV Energy Control

Review of the Status and Trend on HEV Energy Control

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2021中国汽车工程学会年会暨展览会

作者： Tang xuelin Li Qian Wu Xuesong Yu Tao Han Yu Zhen Taoxing Hu Tiegang zhang Xiaoyu zhan zhangsong Chongqing Changan Automobile Co. Ltd.

Hybrid electric vehicles（HEV） are favored by the automobile industry because of improving engine combustion performance,reducing emissions,and achieving energy recovery *** can be classified according to the electrification degree and hybrid *** degree can be divided into five types,and hybrid configuration can be divided into three *** energy control strategy is the key to achieve high efficiency and clean optimization of hybrid *** control strategy requires the best match between the vehicle operating conditions,engine,motor,battery and transmission system,taking into account engine emissions,battery life,driving performance and other requirements to achieve the best power distribution and fuel economy of the *** present,there are endless levels of research on hybrid power control strategies and control algorithms,but it can’t meet the complex control system increasingly,joint optimization of multiple control strategies,development of intelligent control algorithms,and the networking and interconnection of hybrid vehicle control systems are the focus of future research.

关键词： hybrid electric vehicle control strategy algorithm optimization

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