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Effects of brain-derived neurotrophic factor on induced differentiation of SH-SY5Y cells in vitro

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Neural Regeneration Research 2009年第12期4卷 1062-1067页

作者： Jiao li Jingqi li xueli li lixia Lu Lei Xu Department of Biochemistry and Molecular Biology Medical School Tongji University Shanghai 200092 China

BACKGROUND： Previous studies have demonstrated that brain-derived neurotrophic factor （BDNF） promotes neural differentiation. However, the mechanisms involved in cell cycle-related protein regulation, which highly correlates to neural proliferation and apoptosis, remain poorly understood. OBJECTIVE： To investigate the effects of various concentrations of BDNF on cycle-related protein mRNA expression in induce-differentiated SH-SY5Y cells in vitro prior to and following G2 phase, and to analyze the neuroprotective effects of BDNF. DESIGN, TIME AND SETTING： A comparison, observational study, based on cell biology, was performed at the Department of Biochemistry, Medical College of Tongji University, from March 2005 to October 2006. MATERIALS： SH-SY5Y cells were provided by Shanghai Institute of Cytology, Chinese Academy of Science; BDNF by Alomone Labs, Israel; all-trans retinoic acid （ATRA） by Sigma-Aldrich, USA. METHODS： SH-SY5Y cells were randomly divided into three groups： blank control [cells were treated in Insulin-Transferrin-Selenium （ITS） solution for 7 days], ATRA （cells were treated with ITS solution containing 10 μmol/L ATRA for 7 days）, and BDNF （cells were treated identical to the ATRA group for 5 days, and then respectively treated in ITS solution containing 1, 10, and 100 μg/L BDNF for 2 days）. The experiment was repeated three times for each group. MAIN OUTCOME MEASURES： mRNA expression levels of cyclin A1, B1, B2, cyclin-dependent kinase 1, and 5 were detected using quantitative real-time RT-PCR; percentage of cells in G1, S, and G2 phases were detected using fluorescence-activated cell sorting. RESULTS： mRNA expression levels of cyclin A1 in the high-dose BDNF group was significantly less than the ATRA group （P 〈 0.05）.mRNA expression levels of cyclin B1 was significantly less in the different BDNF concentration groups compared with the control and ATRA groups （P 〈 0.05 or P 〈 0.01）. mRNA expression levels of cyclin B2 and cyclin-dependent kinase 1

关键词： brain-derived neurotrophic factor induced differentiation cell cycle-related protein quantitative real-time RT-PCR fluorescence-activated cell sorting SH-SY5Y cell line

d-Orbital steered FeN_(4)moiety through N,S dual-site adjustation for zinc-air flow battery

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Journal of Energy Chemistry 2024年第5期92卷 8-15页

作者： Chunzhu Bao Mingwei Tong xueli li Zhonghua Xiang State Key Laboratory of Organic-Inorganic Composites Beijing University of Chemical TechnologyBeijing 100029China

The implementation of pristine covalent organic polymer(CO_(2)P)with well-defined structure as air electrode may spark fresh vitality to rechargeable zinc-air flow batteries(ZAFBs),but it still remains challenges in synergistically regulating their electronic states and structural porosity for the great device ***,we conquer these issues by exploiting N and S co-doped graphene with COP rich in metal-ligand nitrogen to synergistically construct an effective catalyst for oxygen reduction reaction(ORR).Among them,the N and S co-doped sites with high electronegativity properties alter the number of electron occupations in the d orbital of the iron centre and form electron-transfer bridges,thereby boosting the selectivity of the ORR-catalysed four-electron ***,the introduction of COP materials aids the formation of pore interstices in the graphene lamellae,which both adequately expose the active sites and facilitate the transport of reactive *** from the synergistic effect,as-prepared catalyst exhibits excellent half-wave potentials(E_(1/2)=912 mV)and stability(merely 8.8%drop after a long-term durability test of 50000 s).Further,ZAFBs assembled with the N/SG@CO_(2)P catalyst demonstrate exceptional power density(163.8 mW cm^(-2))and continuous charge and discharge for approximately 140 h at 10 mA cm^(-2),outperforming the noble-metal benchmarks.

关键词： Nitrogen/sulfurdual sites Metal-coordinated nitrogen sites Covalent organic polymer Oxygen reduction reaction Zn-airflow battery

Structure optimization of connection frames based on frequency sensitivity in macro-micro motion platforms

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Nanotechnology and Precision Engineering 2019年第1期2卷 40-47页

作者： Lufan Zhang xueli li Haihong Zhang Haixin li Hu li Jun Wu School of Mechanical and Electrical Engineering Henan University of Technology Weihua Group Co. Ltd. School of Mechanical and Electronic Engineering Zhongyuan University of Technology

High-performance connection frames are of great significance for ultra-high acceleration and ultra-precision positioning in macro-micro motion platforms. This paper first takes the connection frame as a research object,builds a finite element model(FEM) of the natural frequency of the frame, and then verifies the correctness of this model. The frequency sensitivity method is then used to perturb the structural parameters of the FEM of the connection frame, and the sensitivities of the first-order natural frequency and mass of the corresponding structural parameters are obtained by calculation and analysis. The design variables are also determined. The natural frequency is used as the optimization objective, and the design parameters and mass of the connection frame are constrained. The structural parameters of the connecting frame are obtained through optimization, and the model is built and verified by experiments. The results show that the first-order natural frequency of the connecting frame is effectively improved by the frequency sensitivity method, avoids resonance between the connecting frame and the voice coil motor, and realizes the lightweight design of the connection frame. This research provides a reliable basis for the stable operation and ultra-precision positioning of ultra-high acceleration macro-motion platforms.

关键词： Ultra-high acceleration Connection frame Natural frequency Sensitivity Structure optimization

A Mass Transfer-Enhanced Pyrolysis-Free Bifunctional Catalyst to Boost Ultralong lifespan Zinc-Air Batteries

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Energy Material Advances 2023年第1期4卷 467-473页

作者： Tengge Chen Di liu xueli li Mengqing Shi Zhonghua Xiang State Key Laboratory of Organic-Inorganic Composites Beijing University of Chemical TechnologyBeijing 100029PR China

The development of efficient and low-cost bifunctional oxygen catalysts is essential yet a challenge for zinc-air batteries(ZABs).Here,we prepared a pyrolysis-free 3-dimensional hybrid catalyst(COP-Fe@3DLDH)with bifunctional oxygen-catalytic activity by integrating layered double hydroxides(3D-LDH)and a fully conjugated iron phthalocyanine-based covalent organic polymer(COP-Fe).More hierarchically porous and larger specific surface area(170.4 m^(2) g^(−1))can effectively enhance the mass transfer process and active site exposure of cathode,allowing COP-Fe@3D-LDH to exhibit an outstanding peak power density(148.5 mW cm^(-2))and an ultralong lifespan(over 400 h at 10 mA cm^(-2))in ***,this work provides a new perspective to enhance the performance of pyrolysis-free catalysts in ZABs.

关键词： pyrolysis hydroxide integrating

Etiological characteristics and treatment of tardive dyskinesia

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Neural Regeneration Research 2006年第6期1卷 549-552页

作者： Zhe li xueli Sun Che Zhou Mental Health Center West China Hospital Sichuan University Chengdu 610041 Sichuan Province China

OBJECTIVE ： The pathogenesis of tardive dyskinesia （TD） is complicated and uncertain, Thus, there is not any effective treatment for it. The psychiatrists pay more and more attention to TD, which lasts for a long time and is difficult to treat. DATA SOURCES： A computer-based online search of Medline database was undertaken to identify articles about the feature of etiology and the progression of treatment for TD published in English by using the keywords of ＂rD, etiology, pathogenesis＂ and ＂TD, therapy, drug treatment＂. Meanwhile, Chinese articles about the feature of etiology and the progression of treatment for TD were searched in Wanfang database and China journal full-text database, and the keywords were ＂TD, etiology, pathogenesis＂ and ＂TD, therapy, drug Treatment＂ in Chinese. STUDY SELECTION： Articles met the following inclusion criteria were selected in this paper. Inclusion criteria：（1） Researches of randomized blind control design, before and after control design and retrospective. （2） Researches of the feature of etiology and the progression of treatment for TD. Exclusion criteria： the repetitive researches and individual reports. DATA EXTRACTION ： Totally 65 articles related the feature of etiology and the progression of treatment for TD of randomized blind control design, before and after control design and retrospective studies were collected, and 53 of them were accorded with the inclusion criteria. Of the 12 excluded ones, 8 were concerning with genetics, 4 were repetitive researches. DATA SYNTHESIS ： The feature of etiology for TD includes：（1） Hypothesis of dopamine receptor super-sensitivity： The dopamine receptor is persistently blocked, so it will result in functional disorder in CNS, and then TD may take place. （2）） Hypothesis of neuronal degeneration： The concentration of aminosuccinic acid and glutamic acid will increase after the antipsychotic used for a long time and this will result in neuronal degeneration through glutamic acid rec

关键词： dopamine receptor neuronal degeneration control design therapeutic effect free radicals radical scavenger gene polymorphism prevention of glutamic acid retrospective studies tardive dyskinesia cytotoxic effect corpus striatum calcium ions nerve cells amino acid vitamin E

Nutrient uptake,physiological responses and growth of tobacco(Nicotiana tabacum L.)in soil under composite salt stress

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Pedosphere 2022年第6期32卷 893-904页

作者： Jian CUI Dongrui YAO Jing MA Xiefeng YE Ying PENG Jiaqian SONG Jinfeng li Yajun CHANG John YANG Zhen ZHANG xueli li Xiaojing liU Khalil KARIMAN Institute of Botany Jiangsu Province and Chinese Academy of SciencesNanjing 210014(China) National Tobacco Cultivation and Physiology and Biochemistry Research Center Key Laboratory for Tobacco Cultivation of Tobacco IndustryCollege of Tobacco ScienceHenan Agricultural UniversityZhengzhou 450002(China) Shangluo Tobacco Company Luonan BranchShangluo 726000(China) School of Environmental Science Nanjing Xiaozhuang UniversityNanjing 211171(China) Department of Agriculture and Environmental Science Lincoln University of MissouriJefferson City MO 65201(USA) Staff Development Institute of China National Tobacco Corporation Zhengzhou 450000(China) School of Agriculture and Environment The University of Western AustraliaPerth WA 6009(Australia)

High soil salinity imposes osmotic stress and ion toxicity in plants,leading to substantial crop yield loss *** of the quantitative and dynamic physiological responses to composite soil salt stress is limited and needs to be *** this study,physiological,nutritional,and biomass yield parameters of tobacco(Nicotiana tabacum L.)grown in soil with five levels of composite soil salinity(CSS),basal CSS level(control,CK)and 3(T_(1)),6(T_(2)),9(T_(3)),and 12(T_(4))times the basal CSS level,under greenhouse were determined at days 30,60,and 90 after *** dry biomass significantly(Plinity levels applied(T_(1) and T_(2))at all three time points,whereas it progressively declined as the CSS level further *** leaf physiological and photosynthetic responses were more adversely affected by CSS at the early growth stage(day 30).A path coefficient analysis demonstrated that leaf proline content had the largest direct effect(-0.66),and leaf Cu content had the most significant indirect effect(0.49)on leaf dry biomass of *** results suggest that lower CSS levels(T_(1) and T_(2))could stimulate tobacco growth(leaf biomass yield,in particular),and higher leaf proline and Cu levels at the early growth stage may potentially increase the ability of tobacco plants to withstand the adverse effects of salinity,which could be considered for future research and development of salinity management strategies.

关键词： anti-adversity metabolite composite soil salinity hormone malondialdehyde proline translocation factor

Electron donor–acceptor (D-A) tuning to achieve soluble covalent organic polymers for optoelectronic devices

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eScience 2023年第1期3卷 53-61页

作者： xueli li Xianjin Yue Yifei Wang Tengge Chen Yihui Zhou Di liu Hengyang Xiang Shengli Zhang Haibo Zeng Zhonghua Xiang State Key Laboratory of Organic-Inorganic Composites Beijing University of Chemical TechnologyBeijing100029PR China MIIT Key Laboratory of Advanced Display Materials and Devices Institute of Optoelectronics and NanomaterialsSchool of Materials Science and EngineeringNanjing University of Science and TechnologyNanjing210094PR China

Covalent organic polymers(COPs)have emerged as a unique class of luminescent polymers with pre-designed quasi-ordered ***,their layered stacks and limited solubility preclude further processing for large-scale applications in devices,especially optoelectronic ***,a universal strategy to adjust the electron donor–acceptor(D-A)moieties of the building blocks in COPs is proposed,achieved by in situ charge exfoliation of COP blocks into few-layer true solutions in(Lewis)acid and base *** electron D-A moieties of the building blocks endow the COPs with the ability to accept or donate electrons,by altering the electron cloud distribution as well as the relative energy levels of the frontier molecular *** resultant soluble COPs can easily be processed into a uniform film by solution processing via the spin-coat *** obtained COP-N achieves efficient and stable perovskite electroluminescence as a novel hole injection material on indium tin oxide,and the operating lifetime for a perovskite quantum dot light-emitting diodes device exceeds that of a poly(ethylene dioxythiophene):polystyrene sulphonate *** straightforward electronic regulation strategy provides a new avenue for the rational synthesis of processable reticular molecular polymers for practical electronic devices.

关键词： Covalent organic polymers Electron donor-acceptor(D-A)moieties In situ charge exfoliation Solution processing

The Diagnostic Value of Oligoclonal Band Detection in Viral Encephalitis

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Journal of Behavioral and Brain Science 2024年第1期14卷 23-31页

作者： Anqi Huang Xianyue Meng xueli li^# Department of Neurology Liaocheng Hospital Affiliated to Shandong First Medical University (Liaocheng People’s Hospital) Liaocheng China

Objective: This study aims to explore the differences in cerebrospinal fluid oligoclonal band (CSF-OCB) expression among different age groups in viral encephalitis and its reference value for diagnosis. Methods: Forty-two patients with viral encephalitis were divided into two groups: 25 adults and 17 children. The presence of oligoclonal bands in the cerebrospinal fluid (CSF) was detected using polyacrylamide gel electrophoresis, and CSF routine analysis was conducted for comparative analysis. Results: The CSF-OCB positivity rate was higher in the adult group (48%) compared with the pediatric group (11.76%), with a statistically significant difference (P Conclusion: 1) The expression of CSF-OCB positivity in patients with viral encephalitis is age-related, with higher positivity rates observed in adults compared to children. 2) Although CSF oligoclonal band detection is not a specific diagnostic marker for viral encephalitis in adults, it still holds certain reference value.

关键词： Oligoclonal Band Detection Viral Encephalitis Routine Analysis of Cerebrospinal Fluid Age

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基于LSTM的布里渊增益谱提取方法

光子学报 2023年第1期52卷 106-115页

作者：尚秋峰李雪丽华北电力大学电子与通信工程系保定071003 华北电力大学河北省电力物联网技术重点实验室保定071003 华北电力大学保定市光纤传感与光通信技术重点实验室保定071003

针对布里渊光时域传感器在进行长距离监测时存在的实时性较差的问题,提出了一种基于长短期记忆网络的布里渊增益谱温度提取方法。采用洛伦兹函数仿真生成用于长短期记忆网络训练的数据集,建立长短期记忆网络与温度的映射关系。搭建了40... 详细信息

针对布里渊光时域传感器在进行长距离监测时存在的实时性较差的问题,提出了一种基于长短期记忆网络的布里渊增益谱温度提取方法。采用洛伦兹函数仿真生成用于长短期记忆网络训练的数据集,建立长短期记忆网络与温度的映射关系。搭建了40 km的布里渊光时域实验系统,通过谱线相减法对实验数据中存在的畸变现象进行校正,采用长短期记忆网络对校正数据进行了温度信息提取。将本文提出的长短期记忆网络方法与经典的极限学习机方法进行了对比。仿真和实验结果表明,所提算法的最小均方根误差可达0.11℃;即使在扫频步长较大的情况下,该方法仍具有较好的测量精度,有利于提高布里渊光时域温度传感系统的实时性。

关键词：光纤传感布里渊光时域分析布里渊增益谱长短期记忆网络温度提取