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中国神经科学学会第十二届全国学术会议

作者： Zhihua yang xueli cai Shaoming Li Pingyi Xu Department of neurology the first affiliated hospital of Guangzhou Medical University

Back group/Objectives: The effects of neuroimmune modulation of BM-MSCs transplantation for stroke by inactivating microglia were proved in some study, but the related mechanism and the time window of the immunomodulation is not clear. Whether BM-MSCs can carry the effect in the acute stage of stroke, whether CD200-CD200 R pathway is involved in the immunomodulation after BM-MSCs transplantation. It is the aim of our study. Method: MCAO（Middle Cerebral Artery Occlusion） rat model was used to evaluate the therapeutic efficacy of BM-MSCs（Bone Marrow-Marrow Stromal Cell） on acute stage of ischemic stroke rats. Activated microglia were evaluated by IBA-1 ***200-CD200 R pathway related mechanism were explored by peri-infarct injection of CD200 R antibody. The effects transplantation on the changes of IBA-1 and CD200 expression were determined by the western blot. Neuronal CD200 coexpression in peri-infarct and BM-MSCs CD200 co-expression were detected by double fluorescence. Neuronal damage was evaluated by Neu N immunobiological stain, Tunel stain and CV stain（Crystal Violet） in the peri-infarct and in ***: Transplanted BM-MSCs can have neuroimmune modulation by inactivate the microglia in peri-infarct by IBA-1 immunoflurences and western blot analysis and partially reversed by CD200 R antibody in the peri-infarct areas. BM-MSCs could migrate to the infarct area, but not in CA1. The control group had a significantly higher infarct zone percentage than that of the transplanted groups. These results suggest that transplanted BM-MSCs can migrate into the peri-infarct zone and significantly reduce the volume of the infarct zone. There were more Neu N and CV positive cells in CA1 and peri-infarct in transplantation group than cell numbers in the same areas in the control group. There were also less Tunnel positive cells in CA1 and in peri-infarct areas in transplant group than in control group. There were more Neuronal CD200 expression and CD200 exp

关键词： Mesenchymal stem cells Microglia Stroke CD200

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Impaired glymphatic system as evidenced by low diffusivity along perivascular spaces is associated with cerebral small vessel disease:a population-based study

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Stroke & Vascular Neurology 2023年第5期8卷 413-423,I0031-I0050页

作者： Yu Tian xueli cai Yijun Zhou Aoming Jin Suying Wang Yingying Yang Lerong Mei Jing Jing Shan Li Xia Meng Tiemin Wei Tao Liu Yongjun Wang Yuesong Pan Yilong Wang Department of Neurology Beijing Tiantan HospitalCapital Medical UniversityBeijingChina China National Clinical Research Center for Neurological Diseases BeijingChina Department of Neurology The Fifth Affiliated Hospital of Wenzhou Medical UniversityLishuiZhejiangChina Beijing Advanced Innovation Center for Biomedical Engineering School of Biological Science and Medical EngineeringBeihang UniversityBeijingChina Cerebrovascular Research Lab The Fifth Affiliated Hospital of Wenzhou Medical UniversityLishuiZhejiangChina Department of Cardiology The Fifth Affiliated Hospital of Wenzhou Medical UniversityLishuiZhejiangChina

Objective This study aims to investigate the associations of glymphatic system with the presence,severity and neuroimaging phenotypes of cerebral small vessel disease(CSVD)in a community-based *** This report included 2219 community-dwelling people aged 50-75 years who participated in the PolyvasculaR Evaluation for Cognitive Impairment and vaScular Events *** diffusivity along perivascular spaces based on diffusion tensor imaging(DTI-ALPS index)was measured to assess glymphatic *** presence and severity of CSVD were estimated using a CSVD score(points from 0 to 6)and a modified CSVD score(points from 0 to 4),which were driven by 4 neuroimaging features of CSVD,including white matter hyperintensity(WMH),enlarged perivascular spaces(EPVS),lacunes,cerebral *** atrophy(BA)was also *** or ordinal logistic regression analyses were carried out to investigate the relationships of DTI-ALPS index with *** The mean age was 61.3(SD 6.6)years,and 1019(45.9%)participants were *** average DTI-ALPS index was 1.67±*** in the first quartile(Q1)of the DTI-ALPS index had higher risks of the presence of CSVD(OR 1.77,95%CI 1.33 to 2.35,p<0.001),modified presence of CSVD(odds ratio(OR)1.80,95%CI 1.38 to 2.34,p<0.001),total burden of CSVD(common OR(cOR)1.89,95%CI 1.43 to 2.49,p<0.001)and modified total burden of CSVD(cOR 1.95,95%CI 1.51 to 2.50,p<0.001)compared with those in the fourth quartile(Q4).Additionally,individuals in Q1 of the DTI-ALPS index had increased risks of WMH burden,modified WMH burden,lacunes,basal ganglia-EPVS and BA(all p<0.05).Conclusion A lower DTI-ALPS index underlay the presence,severity and typical neuroimaging markers of CSVD,implying that glymphatic impairment may interact with CSVD-related pathology in the general ageing population.

关键词： cerebral lymphatic severity

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Microarray Analysis of Differential Gene Expression Profile between Human Fetal and Adult Heart

Microarray Analysis of Differential Gene Expression Profile ...

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第十三届江浙沪儿科学术会议暨2016年浙江省医学会儿科学学术年会

作者： Jue Wang Zhimin Geng Lianhong Jia Jiacheng Lu xueli cai Maoping Chu Department of Cardiac Surgery the First Affiliated Hospital of Wenzhou Medical University Children's Heart Center the Second Affiliated Hospital & Yuying Children's HospitalInstitute of Cardiovascular Development and Translational MedicineWenzhou Medical University Department of Pediatrics the First Affiliated Hospital of Wenzhou Medical University Department of Cardiology the First Affiliated Hospital of Wenzhou Medical University

Objectives:Although many significant physiological changes have been discovered during the maturation process of the heart,the genetic mechanisms involved in the changes between immature and mature myocardium have only been partially ***,high-throughput microarray screening and improved bioinformatics were used to characterize the gene expression profile changed between the human fetal and adult ***:An Arraystar Human Microarray was applied to define gene expression signatures of the fetal hearts(13-17 weeks of gestation,n = 4) and normal adult hearts(30-40 years old,n = 4).Gene ontology analysis,pathway analysis,gene set enrichment analyses and signal transduction network were performed to predict the function of the differentially expressed *** differentially expressed mRNAs were selected and confirmed by quantificational real-time polymerase chain reaction(qRT-PCR).Results:A total of 5547 coding RNAs were found to be significantly differentially expressed(fold change ≥ 2.0,P < 0.05 and false discovery rate < 0.05) between fetal and adult hearts,among which 3,220 mRNAs were downregulated and 2,327 were up-regulated in the adult group compared with the fetal *** ontology analysis revealed that the down-regulated genes were mainly involved in "cell cycle".KEGG pathway analysis and gene set enrichment analyses identified that "cell cycle pathway" was the most enriched pathway involved in the downregulated *** signal transduction network suggested that epidermal growth factor receptor(EFGR) and insulin-like growth factor 1 receptor(IGF1 R) play a central role in the regulation of heart ***,IGF1 R and fibroblast growth factor receptor 2(FGFR2) were identified to be the core genes in the signal transduction network related to cell *** qRT-PCR Results suggested that the expression tendency was concordant with the microarray ***:Taken together,our data identified the transcriptional regul

关键词： Microarray Bioinformatics Human Fetal Adult Heart

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PolyvasculaR Evaluation for Cognitive Impairment and vaScular Events (PRECISE)-a population-based prospective cohort study: rationale, design and baseline participant characteristics

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Stroke & Vascular Neurology 2021年第1期6卷 145-151页

作者： Yuesong Pan Jing Jing xueli cai Yilong Wang Suying Wang Xia Meng Chunlai Zeng Jiong Shi Jiansong Ji Jinxi Lin Lingchun Lyu Zhe Zhang Lerong Mei Shiping Li Shan Li Wanlin Zhu Hao Li Tiemin Wei Yongjun Wang Department of Neurology Beijing Tiantan HospitalCapital Medical UniversityBeijingChina China National Clinical Research Center for Neurological Diseases BeijingChina Department of Neurology Lishui HospitalZhejiang University School of MedicineLishuiChina Cerebrovascular Research Lab Lishui HospitalZhejiang University School of MedicineLishuiChina Department of Cardiology Lishui HospitalZhejiang University School of MedicineLishuiChina Key Laboratory of Imaging Diagnosis and Minimally Invasive Intervention Research Lishui HospitalZhejiang University School of MedicineLishuiChina

Background and purpose Cardiovascular diseases and dementia are two major diseases in the *** is associated with future vascular events and cognitive *** PolyvasculaR Evaluation for Cognitive Impairment and vaScular Events(PRECISE)study is a population-based prospective cohort study with comprehensive evaluation of multiterritorial artery stenosis and plaque using advanced vascular imaging techniques and prospective collection of vascular events and cognitive *** Between May 2017 and September 2019,the PRECISE study enrolled 3067 community-dwelling adults with ages between 50 and 75 years cluster sampled from six villages and four communities of Lishui city in *** are collected in face-to face interviews at baseline,2-year and 4-year follow-up *** MRI including high-resolution sequences for intracranial and carotidal arteries and CT angiography for thoracoabdominal arteries were performed at baseline and will be rescanned after 4 ***/cerebrovascular events and cognitive assessment will be prospectively collected after the *** and urine samples were collected and biomarkers were tested at *** A total of 3067 subjects were enrolled,among which 53.5%were female with an average age of 61.2±6.7 *** them,2.8%,8.1%,43.1%and 21.6%had a history of stroke,coronary heart diseases,hypertension and diabetes mellitus,*** The PRECISE study is a population-based prospective cohort study with comprehensive evaluation of atherosclerotic stenosis and plaque using advanced vascular imaging *** from this cohort provide us an opportunity to precisely evaluate polyvascular atherosclerosis and its association with future vascular events and cognitive impairment.

关键词： prospective Blood PRECIS

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Safety of endovascular therapy for symptomatic intracranial artery stenosis:a national prospective registry

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Stroke & Vascular Neurology 2022年第2期7卷 166-171页

作者： Yabing Wang Tao Wang Adam Andrew Dmytriw Kun Yang Liqun Jiao Huaizhang Shi Jie Lu Tianxiao Li Yujie Huang Zhenwei Zhao Wei Wu Jieqing Wan Qinjian Sun Bo Hong Yongli Li Liyong Zhang Jianfeng Chu Qiong Cheng Yiling cai Pengfei Wang Qi Luo Hua Yang Baijing Dong Yang Zhang Jun Zhao Zuoquan Chen Wei Li Xiaoxin Bai Weiwen He xueli cai Maimai Ti Osama O Zaidat Department of Neurosurgery Xuanwu HospitalBeijingChina Neuroendovascular Program Massachusetts General HospitalBostonMassachusettsUSA Department of Evidence-Based Medicine Xuanwu HospitalBeijingChina Department of Neurosurgery First Affiliated Hospital of Harbin Medical UniversityHarbinHeilongjiangChina Department of Radiology Xuanwu HospitalBeijingChina Center of Neurointerventional Therapy Henan Provincial People's HospitalZhengzhouChina Department of Neurology Nanjing Drum Tower HospitalNanjingJiangsuChina Department of Neurosurgery Tangdu Hospital Fourth Military Medical UniversityXi'anShaanxiChina Department of Neurology Shandong University Qilu HospitalJinanShandongChina Department of Neurosurgery Shanghai Jiao Tong University School of Medicine Affiliated Renji HospitalShanghaiShanghaiChina Department of Neurology Shandong Provincial HospitalJinanShandongChina Neusurgery Changhai HospitalShanghaiChina Department of Neurosurgery Second Affiliated Hospital of Harbin Medical UniversityHarbinHeilongjiangChina Department of Neurosurgery Liaocheng People's HospitalLiaocheng CityShandongChina Department of Neurosurgery Jining First People's HospitalJiningShandongChina Department of Neurology Fujian Provincial HospitalFuzhouFujianChina Department of Neurology 306 Hospital of People's Liberation ArmyBeijingChina Department of Neurosurgery Tianjin Huanhu HospitalTianjinTianjinChina Department of Neurosurgery Jilin University First HospitalChangchunJilinChina Department of Neurology Affiliated Hospital of Guiyang Medical CollegeGuiyangGuizhouChina Department of Neurosurgery Fourth Affiliated Hospital of Harbin Medical UniversityHarbinHeilongjiangChina Department of Neurosurgery Peking University First HospitalBeijingBeijingChina Department of Neurology Liaocheng Second People's HospitalLiaochengChina Department of Neurology Shanghai Tenth People's HospitalShanghaiChina Department of Neurosurgery Tianjin Medical University General HospitalTianjinTianjin

Introduction The safety outcomes of endovascular therapy for intracranial artery stenosis in a real-world stetting are largely *** Clinical Registration Trial of Intracranial Stenting for Patients with Symptomatic Intracranial Artery Stenosis(CRTICAS)was a prospective,multicentre,real-world registry designed to assess these outcomes and the impact of centre *** 1140 severe,symptomatic intracranial arterial stenosis(ICAS)patients treated with endovascular therapy were included from 26 centres,further divided into three groups according to the annual centre volume of intracranial angioplasty and stent placement procedures over 2 years:(1)high volume for≥25 cases/year;(2)moderate volume for 10–25 cases/year and(3)low volume for<10 cases/*** The rate of 30-day stroke,transient ischaemic attack or death was 9.7%(111),with 5.4%,21.1%and 9.7%in high-volume,moderate-volume and low-volume centres,respectively(p<0.05).Multivariable logistic regression confirmed high-volume centres had a significantly lower primary endpoint compared with moderate-volume centres(OR=0.187,95%CI:0.056 to 0.627;p≤0.0001),while moderate-volume and low-volume centres showed no significant difference(p=0.8456).Conclusion Compared with the preceding randomised controlled trials,this real-world,prospective,multicentre registry shows a lower complication rate of endovascular treatment for symptomatic ***-uniform utilisation in endovascular technology,institutional experience and patient selection in different volumes of centres may have an impact on overall safety of this treatment.

关键词： intracranial prospective stenosis

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掺入大肠杆菌膜中的磷脂酰胆碱(PC)影响青霉素β-内酰胺酶的分泌

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微生物学报 2008年第4期48卷 486-491页

作者：蔡雪丽李洋宣文静王行国湖北大学生命科学学院武汉430062

【目的】为了研究磷脂酰胆碱（PC）在原核生物细胞中的生物学作用，探讨PC对细菌膜系统的功能的影响。【方法】使用p^tac85质粒作载体，将螺旋菌pcs基因导人*** Top10细胞构建了*** Top10 pcs^＋菌株，并在特定的条件下培养细菌，使细... 详细信息

【目的】为了研究磷脂酰胆碱（PC）在原核生物细胞中的生物学作用，探讨PC对细菌膜系统的功能的影响。【方法】使用p^tac85质粒作载体，将螺旋菌pcs基因导人*** Top10细胞构建了*** Top10 pcs^＋菌株，并在特定的条件下培养细菌，使细菌膜磷脂中合成30％左右的磷脂酰胆碱。然后再使用抗生素抗性分析、β-内酰胺酶的酶活测定以及Western blot杂交技术，分析质粒编码的β-内酰胺酶从细胞质到细胞间质的分泌情况。【结果】抗生素抗性分析发现，高浓度的氨苄青霉素抑制*** Top10 pcs^＋细菌的生长的氨苄青霉素剂量低于对照组，其半致死剂量IC50在700～800μg／mL之间。酶活检测显示*** Top10 pcs^＋细菌周质内β-内酰胺酶的酶活性只有对照菌株的1／5，Western blot进一步分析发现周质内β-内酰胺酶的含量也为对照菌株的1／5。由此可见，周质内低含量的β-内酰胺酶是导致*** Top10 pcs^＋细菌氨苄青霉素抗性降低的原因。【结论】掺人细菌膜磷脂双分子层的PC影响β-内酰胺酶通过Sec转运途径从细胞质分泌到细菌周质空间内，提示细菌磷脂酰胆碱可能在调节蛋白转运和分泌方面起着重要的作用。

关键词：磷脂酰胆碱 β-内酰胺酶氨苄青霉素蛋白分泌

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受顺序影响的证真偏向:来自四卡问题新范式的证据

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心理与行为研究 2017年第1期15卷 54-61页

作者：蔡雪丽吕辉李富洪李红西南交通大学心理研究与咨询中心成都610031 西南交通大学公共管理与政法学院成都610031 江西师范大学心理学院江西330022 深圳大学心理与社会学院深圳518060

通过对经典四卡问题呈现方式的改进,逐一呈现与命题对应的检验证据,要求被试判断每一证据在命题检验中的作用。结果表明:(1)"证真偏向"依然存在,但从正确率来看,顺证真与顺证伪没有显著差异;(2)发现了显著的顺序效应,与逆向检验相比,顺... 详细信息

通过对经典四卡问题呈现方式的改进,逐一呈现与命题对应的检验证据,要求被试判断每一证据在命题检验中的作用。结果表明:(1)"证真偏向"依然存在,但从正确率来看,顺证真与顺证伪没有显著差异;(2)发现了显著的顺序效应,与逆向检验相比,顺向检验有更高的正确率与更短的反应时;(3)被试加工与命题"无关联"刺激时存在明显困难,其正确率显著较低。这些结果说明个体在判断不同证据对命题检验的作用时会受到命题内在方向性的影响,个体可能会采用一些固有推理图式以及简单的匹配策略解决问题。

关键词：证真偏向顺序效应匹配策略

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Exploration on the Path of Whole-region Comprehensive Land Consolidation under the Background of Rural Revitalization

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Asian Agricultural Research 2023年第6期15卷 1-6页

作者： xueli cai Shiqin YANG Institute of Land&Resources and Sustainable Development Yunnan University of Finance and EconomicsKunming 650221China

Based on the specific concept of whole-region comprehensive land consolidation and the development history of land consolidation in China,the internal relationship between rural revitalization strategy and whole-region comprehensive land consolidation is clarified from the perspective of historical development,and the problems faced by whole-region comprehensive land consolidation,such as rural"hollowing out",weak linkage between execution departments and neglect of ecological restoration,are deeply ***,some new paths for whole-region land consolidation are put forward,such as strengthening the top-level system design,handling the relationship between stock and increment,evaluating the potential of rural land consolidation,coordinating the win-win cooperation between middle-level executive departments,encouraging the innovation at the bottom and enhancing the comprehensive management of ecological environment.

关键词： Rural revitalization Whole-region comprehensive land consolidation Path Exploration

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短时记忆定向遗忘的负荷效应:来自ERP的证据

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心理科学 2015年第3期38卷 514-520页

作者：蔡雪丽高贺明曹碧华李富洪西南大学心理学部重庆400715 辽宁师范大学心理学院大连116029

采用事件相关电位（ERPs）技术,通过一个短时记忆测试,考察记忆/遗忘线索在不同的工作记忆负荷条件下诱发的脑电差异。实验以字母和数字为刺激,要求被试记忆或遗忘不同数量的视觉信息。ERP结果发现：在线索呈现230~430ms的时间窗口,高... 详细信息

采用事件相关电位（ERPs）技术,通过一个短时记忆测试,考察记忆/遗忘线索在不同的工作记忆负荷条件下诱发的脑电差异。实验以字母和数字为刺激,要求被试记忆或遗忘不同数量的视觉信息。ERP结果发现：在线索呈现230~430ms的时间窗口,高负荷条件的遗忘线索比低负荷条件的遗忘线索诱发了更正的ERPs。这一结果表明,抑制机制在定向遗忘中有重要作用,抑制不同数量的信息需要不同数量的认知资源。

关键词：定向遗忘认知负荷抑制 ERP

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景观格局因子交互作用对科尔沁沙地沙漠化的影响

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中国沙漠 2024年第2期44卷 99-108页

作者：蔡明玉贾飚常学礼烟台工程职业技术学院山东烟台264006 内蒙古自治区水利科学研究院内蒙古呼和浩特010051 鲁东大学资源与环境工程学院山东烟台264025

将景观生态学原理运用到沙漠化现状的准确和快捷评估是景观生态学研究面临的一个挑战。本文以2000年和2018年奈曼旗中部沙区土地利用类型数据为基础,选定最大斑块指数(LPI)、边界密度(ED)、聚集度指数(AI)和香农指数(SHDI),借助地理探... 详细信息

将景观生态学原理运用到沙漠化现状的准确和快捷评估是景观生态学研究面临的一个挑战。本文以2000年和2018年奈曼旗中部沙区土地利用类型数据为基础,选定最大斑块指数(LPI)、边界密度(ED)、聚集度指数(AI)和香农指数(SHDI),借助地理探测器模型和Fragstats软件中的Moving window功能分析了景观格局双因子交互作用及因子分级对沙漠化现状的影响。结果表明:2000—2018年是研究区沙漠化逆转期,沙漠化现状指数由0.393减少到0.288。景观格局双因子交互作用有非线性增强和双因子增强两种形式且对沙漠化现状的影响大于各因子独立作用。不同格局因子分级对沙漠化现状影响存在差异,导致差异原因与不同沙丘类型面积和分布格局有关。在沙漠化现状较强年份(2000年),LPI和ED分级导致的沙漠化现状显著区,以流动沙丘占优势(30.6%~42.9%),不显著区以固定沙丘占优势(32.0%~44.0%)。在沙漠化现状较弱年份(2018年),SHDI分级导致的沙漠化现状显著区和不显著区都以流动沙丘占优势(均超过79.2%)。景观格局双因子交互对沙漠化现状影响既与时间有关,又与空间范围有关;交互作用对沙漠化现状的影响机制具有非线性特点;研究区范围大小直接影响到景观因子交互作用形式和空间格局(尺度依赖),任何典型案例研究结果不能跨尺度推绎,但方法可以统一。

关键词：地理探测器移动窗口法景观格局沙漠化现状科尔沁沙地

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