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The initial attempt to reveal the emission processes of both mechanoluminescence and room temperature phosphorescence with the aid of circular dichroism in solid state

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Science China Chemistry 2021年第3期64卷 445-451页

作者： Yu Tian xuekang yang Yanbin Gong Yunsheng Wang Manman Fang Jie yang Zhiyong Tang Zhen Li Institute of Molecular Aggregation Science Tianjin UniversityTianjin 300072China Department of Chemistry Wuhan UniversityWuhan 430072China Joint School of National University of Singapore and Tianjin University International Campus of Tianjin UniversityBinhai New CityFuzhou 350207China CAS Key Laboratory of Nanosystem and Hierarchical Fabrication CAS Center for Excellence in NanoscienceNational Center for Nanoscience and TechnologyBeijing 100190China

A phenothiazine derivative PtzChol containing non-conjugated chiral cholesterol group was designed and synthesized. By analyzing the single crystal structure of PtzChol carefully, coupled with the circular dichroism(CD) signals before and after grinding, it was found that the introduction of cholesterol produced a positive effect on the production of chiral space group, on mechanoluminescence(ML) and room temperature phosphorescence(RTP), and throughout the entire light-emitting process, the CD signal could well reflect the changes of molecular arrangement.

关键词： mechanoluminescence room temperature phosphorescence circular dichroism phenothiazine cholesterol

A fluorogenic-inhibitor-based probe for profiling and imaging of monoamine oxidase A in live human glioma cells and clinical tissues

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Science China Chemistry 2023年第7期66卷 2053-2061页

作者： Haixiao Fang Panpan Li Congzhen Shen Fang Tang Aixiang Ding Hua Bai Bo Peng xuekang yang Zhengqiu Li Kai Huang Sijun Pan Lin Li Wei Huang The Institute of Flexible Electronics(IFE Future Technologies)Xiamen UniversityXiamen 361005China Future Display Institute in Xiamen Xiamen 361005China Frontiers Science Center for Flexible Electronics Xi’an Institute of Flexible Electronics(IFE)and Xi’an Institute of Biomedical Materials&EngineeringNorthwestern Polytechnical UniversityXi’an 710072China School of Pharmacy Jinan UniversityGuangzhou 510632China Xijing Hospital The Fourth Military Medical UniversityXi’an 710032China

Monoamine oxidase A(MAO-A)plays a critical role in the development of glioma and other neurological *** analysis of MAO-A activities and its drug interactions in intact tissue is important for biological and pharmacological research,but highly challenging with current chemical ***-inhibitor-based probes offer improved selectivity,sensitivity,and effectiveness to image and profile endogenous targets in an activity-based manner from mammalian cells,which are however ***,we report HD1 as the first fluorogenic-inhibitor-based probe that can selectively label endogenous MAO-A from various mammalian cells and clinical *** probe was delicately designed based on N-propargyl tetrahydropyridine,a small MAO-A-specific fluorogenic and inhibitory war-head,so that the probe becomes fluorescent upon in situ enzymatic oxidation and covalent labeling of *** the excellent binding affinity(in vitro K_(i)=285 n M)and fluorogenic properties,HD1 offers a promising approach to simultaneously image endogenous MAO-A activities by super-resolution fluorescence microscopy and study its drug interactions by subsequent activity-based protein profiling,in both live cells and human glioma tissues.

关键词： monoamine oxidase A fluorogenic-inhibitor-based probe super-resolution imaging activity-based protein profiling glioma

The Notch pathway attenuates burn-induced acute lung injury in rats by repressing reactive oxygen species

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Burns & Trauma 2022年第1期10卷 570-585页

作者： Weixia Cai Kuo Shen Peng Ji Yanhui Jia Shichao Han Wanfu Zhang Xiaolong Hu xuekang yang Juntao Han Dahai Hu Department of Burns and Cutaneous Surgery Xijing HospitalFourth Military Medical UniversityXi’an 710032China

Background:Acute lung injury(ALI)is a common complication following severe *** underlying mechanisms of ALI are incompletely understood;thus,available treatments are not sufficient to repair the lung tissue after ***:To investigate the relationship between the Notch pathway and burn-induced lung injury,we established a rat burn injury model by scalding and verified lung injury via lung injury evaluations,including hematoxylin and eosin(H&E)staining,lung injury scoring,bronchoalveolar lavage fluid and wet/dry ratio analyses,myeloperoxidase immunohistochemical staining and reac-tive oxygen species(ROS)accumulation *** explore whether burn injury affects Notch1 expression,we detected the expression of Notch1 and Hes1 after burn ***,we extracted pulmonary microvascular endothelial cells(PMVECs)and conducted Notch pathway inhibition and activation experiments,via aγ-secretase inhibitor(GSI)and OP9-DLL1 coculture,respectively,to verify the regulatory effect of the Notch pathway on ROS accumulation and apoptosis in burn-serum-stimulated *** investigate the regulatory effect of the Notch pathway on ROS accumulation,we detected the expression of oxidative-stress-related molecules such as superoxide dismutase,nicotinamide adenine dinucleotide phosphate(NADPH)oxidase(NOX)2,NOX4 and cleaved ***4-specific small interfering RNA(siRNA)and the inhibitor GKT137831 were used to verify the regulatory effect of the Notch pathway on ROS via ***:We successfully established a burn model and revealed that lung injury,excessive ROS accumulation and an inflammatory response ***1 detection showed that the expression of Notch1 was significantly increased after burn *** PMVECs challenged with burn serum,ROS and cell death were ***,when the Notch pathway was suppressed by GSI,ROS and cell apoptosis levels were significantly ***,these parameters were reduced when the Notch pathway was activated

关键词： Acute lung injury Notch pathway Reactive oxygen species Pulmonary microvascular endothelial cells Nicotinamide adenine dinucleotide phosphate oxidase 4 Burn

Adoptive transfer of metabolically reprogrammed macrophages for atherosclerosis treatment in diabetic ApoE^(-/-) mice

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Bioactive Materials 2022年第10期7卷 82-94页

作者： Tingting Wang Yan Dong Li Yao Fan Lu Chenxi Wen Zhuo Wan Li Fan Zhelong Li Te Bu Mengying Wei xuekang yang Yi Zhang Department of Equipment Division School of StomatologyFourth Military Medical UniversityXi’an710032People’s Republic of China Department of Burns and Cutaneous Surgery Xijing HospitalFourth Military Medical UniversityXi’an710032People’s Republic of China Department of Hematology Tangdu HospitalFourth Military Medical UniversityXi’an710038People’s Republic of China Department of Pathology Xi’an No.3 HospitalThe Affiliated Hospital of Northwest UniversityXi’an710018People’s Republic of China Department of Biochemistry and Molecular Biology Fourth Military Medical UniversityXi’an710032People’s Republic of China Department of Ultrasound Diagnostics Tangdu HospitalFourth Military Medical UniversityXi’an710038People’s Republic of China

Atherosclerosis is characterized by inflammation in the arterial wall,which is known to be exacerbated by *** repression of inflammation is a promising strategy for treating *** this study,we showed that diabetes aggravated atherosclerosis in apolipoproteinE knockout(ApoE^(-/-))mice,in which increased expression of long-chain acyl-CoA synthetase 1(Acsl1)in macrophages played an important *** of Acsl1 in macrophages(Mφ^(shAcsl1))reprogrammed macrophages to an anti-inflammatory phenotype,especially under hyperglycemic *** of Mφ^(shAcsl1) reprogrammed macrophages into streptozotocin(STZ)-induced diabetic ApoE^(-/-) mice(ApoE^(-/-)+STZ)alleviated inflammation locally in the plaque,liver and *** with the reduction in inflammation,plaques became smaller and more stable after the adoptive transfer of reprogrammed *** together,our findings indicate that increased Acsl1 expression in macrophages play a key role in aggravated atherosclerosis of diabetic mice,possibly by promoting *** transfer of Acsl1 silenced macrophages may serve as a potential therapeutic strategy for atherosclerosis.

关键词： Atherosclerosis Diabetes Macrophage Acsl1 Adoptive transfer

Study on Design and Fabrication of Micro Thermal Control Louvers

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稀有金属材料与工程 2011年第S4期40卷 249-251页

作者： Cao Shengzhu,Chen xuekang,Wu Gan,yang Jianping,Wang Rui,Wang Lanxi,Shang Kaiwen Science and Technology on Surface Engineering Laboratory,Lanzhou Institute of Physics,Lanzhou 730000,China Lanzhou Inst Phys Sci & Technol Surface Engn Lab Lanzhou 730000 Peoples R China

A micro thermal control device with polyimide based micro louver was *** device structure was designed,the actuation voltage was analyzed theoretically and fabrication process was *** micro louver prototype was fabricated using UV laser micromachining,electrochemistry etch and magnetron sputtering deposition *** main parameters were tested and results were presented.

关键词： micro louvers micromachining thermal control

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严重烧伤患者的营养治疗研究进展

中华烧伤杂志 2016年第10期32卷 628-631页

作者：陈俏华杨薛康胡大海第四军医大学西京医院全军烧伤中心烧伤与皮肤外科西安710032

近30年来，虽然烧伤的治疗和护理已经取得了长足进步，病死率和后遗症发生率大大降低，但对于烧伤总面积超过30％TBSA伴或不伴吸入性损伤的严重烧伤患者的救治，仍是一个难题。此类烧伤患者常伴有长达数月的代谢亢进或分解代谢异常，以... 详细信息

近30年来，虽然烧伤的治疗和护理已经取得了长足进步，病死率和后遗症发生率大大降低，但对于烧伤总面积超过30％TBSA伴或不伴吸入性损伤的严重烧伤患者的救治，仍是一个难题。此类烧伤患者常伴有长达数月的代谢亢进或分解代谢异常，以及强烈的氧化应激反应和炎症反应等。因此，营养治疗是烧伤复苏初期必不可少的一步。本文对营养治疗的相关问题进行了综述，旨在对临床_T作起到一定的参考作用。

关键词：烧伤肠道营养能量代谢

钠氢交换蛋白1抑制剂对烧伤脓毒症大鼠肠道损伤的作用及其机制

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中华烧伤杂志 2017年第6期33卷 349-354页

作者：李文鹏赵高炀杨薛康第四军医大学学员旅西安710032 第四军医大学西京医院全军烧伤中心烧伤与皮肤外科

目的观察钠氢交换蛋白1（NHE1）抑制剂对烧伤脓毒症大鼠肠道损伤的作用，并初步探讨其可能机制。方法取SD大鼠90只，按照随机数字表法分为对照组、单纯脓毒症组和NHE1抑制剂组，每组30只。单纯脓毒症组和NHE1抑制剂组大鼠背部建立20％T... 详细信息

目的观察钠氢交换蛋白1（NHE1）抑制剂对烧伤脓毒症大鼠肠道损伤的作用，并初步探讨其可能机制。方法取SD大鼠90只，按照随机数字表法分为对照组、单纯脓毒症组和NHE1抑制剂组，每组30只。单纯脓毒症组和NHE1抑制剂组大鼠背部建立20％TBSAⅢ度烫伤（下称烧伤）模型后，背部创面中心注射2×10^5CFU／mL的铜绿假单胞菌ATCC27853菌液50μL。烧伤脓毒症模型建立成功后，NHE1抑制剂组大鼠迅速腹腔注射0．1mmol／LNHE1抑制剂卡立泊来德0．4mg／kg，单纯脓毒症组大鼠注射同体积的生理盐水。对照组大鼠除不制作烧伤创面和接种细菌外，其余操作与单纯脓毒症组大鼠相同。烧伤脓毒症大鼠在伤后12h剖腹，于回肠远端注射0．1mol／L异硫氰酸荧光素（FITC）-葡聚糖200mL，30min后左心室采血，切取回肠末端组织。荧光分光光度计检测大鼠血清FITC-葡聚糖含量（样本数为10），HE染色观察肠道组织形态（样本数为10），ELISA法检测血清和肠道组织IL-6和TNF—α含量（样本数均为20），比色法检测血清和肠道组织髓过氧化物酶（MPO）活性（样本数均为20），蛋白质印迹法检测肠道组织NF-κB-p65蛋白表达及MAPK信号通路相关蛋白p38MAPK、细胞外信号调节激酶1／2（ERK1／2）以及c—Jun氨基末端激酶1／2（JNK1／2）磷酸化水平（样本数为4）。对照组大鼠于相同时相点同前采集标本进行相关检测。对数据行单因素方差分析、SNK检验。结果（1）单纯脓毒症组大鼠血清FITC-葡聚糖含量较对照组明显升高（P〈0．01），NHE1抑制剂组大鼠血清FITC-葡聚糖含量则较单纯脓毒症组显著降低（P〈0．01）。与对照组比较，单纯脓毒症组大鼠肠黏膜可见大量炎性细胞浸润、黏膜溃疡以及黏膜坏死；NHE1抑制剂组大鼠肠道损伤情况较单纯脓毒症组有显著好转。（2）单纯脓毒症组大鼠血清IL-6、TNF-α、MPO含量分别为（387±42）、（164．7±10．1）ng／mL及（7．5±1．5）U／mL，显著高于对照组的（75±17）、（13．1±6．5）ng／mL和（2．3±0．7）U／mL（P值均小于0．01）；NHE1抑制剂组大鼠血清IL-6、TNF—α、MPO含量分别为（176±37）、（64．9±9．3）ng／mL和（5．9±0．8）U／mL，均显著低于单纯脓毒症组（P值均小于0．01）。（3）单纯脓毒症组大鼠肠道组织IL-6、TNF—α、MPO含量分别为（190±13）、（172．8±29．7）ng／mL及（8．7±1．5）U／mL，显著高于对照组的（20±3）、（11．9±2．3）ng／mL和（2．9±0．3）U／mL（P值均小于0．01）；NHE1抑制剂组大鼠肠道组织IL-6、TNF-α、MPO含量分别为（35±6）、（45．2±6．1）ng／mL和（5．3±0．6）U／mL，均显著低于单纯脓毒症组（P值均小于0．01）。（4）单纯脓毒症组大鼠肠道组织NF—κB—p65蛋白表达量和p38MAPK、ERK1／2磷酸化水平显著高于对照组（P值均小于0．01），NHEI抑制剂组大鼠肠道组织NF-κB—p65蛋白表达量和p38MAPK磷酸化水平明显低于单纯脓毒症组（P值均小于0．01），3组大鼠肠道组织JNKl／2磷酸化水平无明显差异（P值均大于0．05）。结论抑制NHE1可显著减轻烧伤脓毒症诱导的大鼠肠道损伤，其机制可能为通过调控NF—κB及p38MAPK信号通路，抑制肠道炎症反应。

关键词：烧伤脓毒症炎症过氧化物酶肠道损伤丝裂原活化蛋白激酶钠氢交换蛋白1

BAY11-7082对严重烧伤大鼠急性肺损伤及早期肺炎症反应的干预作用

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中华烧伤杂志 2018年第2期34卷 88-95页

作者：李虎山杨薛康郝振明雷晋山西医科大学第六医院烧伤科、山西省烧伤救治中心太原030009 空军军医大学西京医院全军烧伤中心、烧伤与皮肤外科

目的探讨BAY11－7082对严重烧伤大鼠急性肺损伤及早期肺炎症反应的干预作用。方法（1）实验1。取12只SD大鼠，按随机数字表法分为正常对照组3只、烧伤组9只。正常对照组大鼠不行特殊处理；烧伤组大鼠背部造成30%体表总面积Ⅲ度烧伤，伤... 详细信息

目的探讨BAY11－7082对严重烧伤大鼠急性肺损伤及早期肺炎症反应的干预作用。方法（1）实验1。取12只SD大鼠，按随机数字表法分为正常对照组3只、烧伤组9只。正常对照组大鼠不行特殊处理；烧伤组大鼠背部造成30%体表总面积Ⅲ度烧伤，伤后即刻腹腔注射生理盐水50 mL/kg。烧伤组于伤后12、24、48 h分别取3只大鼠采集腹主动脉血并取肺组织，酶联免疫吸附测定法检测血清中白细胞介素1β（IL－1β）、IL－18含量，实时荧光定量反转录－聚合酶链反应（RT－PCR）法检测肺组织中IL－1β、IL－18 mRNA表达。正常对照组大鼠同前采样检测。（2）实验2。取18只SD大鼠，按随机数字表法分为正常对照组、单纯烧伤组、BAY11－7082干预组，每组6只。正常对照组大鼠不行特殊处理；单纯烧伤组和BAY11－7082干预组大鼠同实验1致伤，伤后即刻分别腹腔注射生理盐水、终质量浓度为8 mg/mL BAY11－7082溶液50 mL/kg。于实验1结果得出的最佳观察时间点收集烧伤各组大鼠肺组织及肺泡灌洗液，苏木素－伊红染色观察肺组织形态并进行肺组织病理损害评分，酶标仪检测肺组织中髓过氧化物酶（MPO）含量及肺泡灌洗液中总蛋白含量，蛋白质印迹法检测肺组织中半胱氨酸天冬氨酸蛋白酶1和核苷酸结合寡聚化结构域样受体3（NLRP3）的蛋白表达，实时荧光定量RT－PCR法检测肺组织中IL－1β、IL－18、NLRP3、半胱氨酸天冬氨酸蛋白酶1 mRNA表达。正常对照组大鼠同前采样检测。对数据行单因素方差分析、LSD－t检验。结果（1）烧伤组大鼠伤后12、24、48 h血清中IL－1β和IL－18含量显著高于正常对照组（t＝10.55、22.05、12.47、10.60、15.22、11.94，P〈0.01）。烧伤组大鼠伤后12、24、48 h肺组织中IL－1β和IL－18 mRNA表达量明显高于正常对照组（t＝3.62、7.19、5.28、3.20、12.62、7.31，P〈0.05或P〈0.01）。伤后24 h为后续实验最佳观察时间点。（2）伤后24 h，与单纯烧伤组比较，BAY11－7082干预组大鼠炎性细胞浸润及肺泡内红细胞渗出明显减少，肺间质水肿明显消退。单纯烧伤组大鼠肺组织病理损害评分为（9.00±1.00）分，显著高于正常对照组的（1.10±0.26）分（t＝13.23，P〈0.01）。BAY11－7082干预组大鼠肺组织病理损害评分为（4.93±0.70）分，虽仍高于正常对照组（t＝8.84，P〈0.01），但较单纯烧伤组明显降低（t＝5.76，P〈0.01）。伤后24 h，单纯烧伤组大鼠肺组织中MPO含量[（1.83±0.15）U/mg]及肺泡灌洗液中总蛋白含量[（1.39±0.20）mg/mL]均显著高于正常对照组[（0.51±0.10）U/mg、（0.44±0.05）mg/mL，t＝12.50、7.86，P〈0.01]，BAY11－7082干预组大鼠肺组织中MPO含量[（0.91±0.12）U/mg]及肺泡灌洗液中总蛋白含量[（0.60±0.10）mg/mL]显著低于单纯烧伤组（t＝8.36、6.06，P〈0.01）。伤后24 h，单纯烧伤组大鼠肺组织中NLRP3和半胱氨酸天冬氨酸蛋白酶1蛋白表达量分别为3.10±0.09、2.99±0.30，显著高于正常对照组的1.00、1.00（t＝9.06、11.28，P〈0.01）；BAY11－7082干预组大鼠肺组织中NLRP3和半胱氨酸天冬氨酸蛋白酶1蛋白表达量分别为1.13±0.08、1.81±0.11，显著低于单纯烧伤组（t＝7.24、3.91，P〈0.05或P〈0.01）。伤后24 h，单纯烧伤组大鼠肺组织中IL－1β、IL－18、NLRP3和半胱氨酸天冬氨酸蛋白酶1的mRNA表达量分别为5.0±0.4、3.32±0.21、3.54±0.42、6.3±1.0，明显高于正常对照组的1.0、1.00、1.00、1.0（t＝13.97、14.14、11.78、7.13，P〈0.01）；BAY11－7082干预组大鼠肺组织中IL－1β、IL－18、NLRP3和半胱氨酸天冬氨酸蛋白酶1的mRNA表达量（2.6±0.5、2.00±0.28、1.39±0.21、2.5±0.5）显著低于单纯烧伤组（t＝7.11、5.80、9.99、4.65，P〈0.05或P〈0.01）。结论严重烧伤大鼠急性肺损伤早期使用BAY11－7082可通过抑制NLRP3，减少半胱氨酸天冬氨酸蛋白酶1的表达，显著降低IL－1β和IL－18的水平，同时减少肺组织中MPO含量及肺泡灌洗液中总蛋白含量，从而减轻肺炎症反应，减轻肺损伤。

关键词：烧伤急性肺损伤炎症半胱氨酸天冬氨酸蛋白酶1 白细胞介素1β 白细胞介素18 核苷酸结合寡聚化结构域样受体3

足底内侧游离皮瓣整复手掌电烧伤创面及瘢痕挛缩的疗效

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中华烧伤与创面修复杂志 2023年第9期39卷 820-825页

作者：韩夫杨薛康何亭王路旭张楠韩军涛空军军医大学第一附属医院全军烧伤中心烧伤与皮肤外科西安710032

目的探讨足底内侧游离皮瓣整复手掌电烧伤创面及瘢痕挛缩的疗效。方法采用回顾性观察性研究方法。2020年1月—2023年1月,空军军医大学第一附属医院收治6例符合入选标准的手掌电烧伤创面或瘢痕挛缩患者,其中男5例、女1例,年龄35~55岁。... 详细信息

目的探讨足底内侧游离皮瓣整复手掌电烧伤创面及瘢痕挛缩的疗效。方法采用回顾性观察性研究方法。2020年1月—2023年1月,空军军医大学第一附属医院收治6例符合入选标准的手掌电烧伤创面或瘢痕挛缩患者,其中男5例、女1例,年龄35~55岁。手掌电烧伤创面清创后及瘢痕切除后创面面积为5.0 cm×3.0 cm~8.0 cm×7.0 cm。均采用吻合皮神经的足底内侧游离皮瓣整复创面,皮瓣面积为5.5 cm×3.5 cm~8.5 cm×7.5 cm。移植腹部全厚皮修复皮瓣供区创面。术后观察皮瓣和皮片存活情况,随访观察皮瓣外形、质地及皮瓣供区情况,评估患手握持功能恢复情况。于末次随访时,测量皮瓣两点辨别觉距离并按照中华医学会手外科学会上肢部分功能评定试用标准评定皮瓣感觉恢复情况,采用皮瓣术后功能评价量表评价皮瓣功能恢复情况。结果术后5个皮瓣存活良好;1个皮瓣远端部分坏死,清创后移植大腿外侧中厚皮修复。皮瓣供区皮片均存活良好。随访3~24个月,皮瓣不臃肿,质地、色泽好,与周围组织匹配度高;皮瓣供区无明显瘢痕挛缩发生;患手握持功能良好。末次随访时,皮瓣两点辨别觉距离为6~8 mm,皮瓣感觉恢复:5个皮瓣恢复到S3+级、1个皮瓣恢复到S3级,皮瓣功能评定:优5个、良1个,患者基本恢复正常的生活和工作。结论吻合皮神经的足底内侧游离皮瓣整复手掌电烧伤创面及瘢痕挛缩具有术后外观匹配度高、感觉恢复良好及患手握持功能良好等诸多优点,是整复手掌电烧伤创面和瘢痕挛缩的理想选择。

关键词：烧伤,电手损伤瘢痕外科皮瓣显微外科手术创面修复