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China CDC weekly 2021年第46期3卷 973-976页

作者： Xinxin Shen Jinrong Wang Jingyi Li Anna He Hong Liu xuejun ma National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and PreventionBeijingChina Shijiazhuang People’s Hospital ShijiazhuangHebeiChina Shandong Provincial Research Center for Bioinformatic Engineering and Technique School of Life Sciences and MedicineShandong University of TechnologyZiboShandongChina Center for Biosafety Mega-Science Chinese Academy of SciencesWuhanHubeiChina

ABSTRACT Introduction:The best approach to preventing the importation of coronavirus disease 2019(COVID-19)is enhancing the detection capacity at *** rapid detection is of utmost importance and therefore highly ***:We conducted a field validation study of a duplex real-time reverse transcription recombinaseaided amplification(RT-RAA)assay in Zhoushan and Hangzhou customs,in Zhejiang Province,China.

关键词： customs duplex Customs

Development of an Integrated Disposable Device for SARSCoV-2 Nucleic Acid Extraction and Detection

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Biomedical and Environmental Sciences 2024年第6期37卷 639-646页

作者： Jing ma Yanzhe Hao Meiling Hou Xiaoshan Zhang Jingduan Liu Haodi Meng Jiangbo Chang xuejun ma Jihua Liu Qingjie Ying Xianhua Wang Hongxia Li Yuxi Cao Xiaoguang Zhang National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases National Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China Beijing Runbio Biotechnology Development Co. LtdBeijing 100106China Beijing Beta Technology Co. LtdBeijing 101318China Jiangsu Qitian Gene Biotechnology Co. LtdWuxi 214135JiangsuChina

Objective To develop a highly sensitive and rapid nucleic acid detection method for the severe acute respiratory syndrome coronavirus 2(SARS-CoV-2).Methods We designed,developed,and manufactured an integrated disposable device for SARS-CoV-2 nucleic acid extraction and *** precision of the liquid transfer and temperature control was tested.A comparison between our device and a commercial kit for SARS-Cov-2 nucleic acid extraction was performed using real-time fluorescence reverse transcription polymerase chain reaction(RT-PCR).The entire process,from SARS-CoV-2 nucleic acid extraction to amplification,was *** The precision of the syringe transfer volume was 19.2±1.9μL(set value was 20),32.2±1.6(set value was 30),and 57.2±3.5(set value was 60).Temperature control in the amplification tube was measured at 60.0±0.0℃(set value was 60)and 95.1±0.2℃(set value was 95)***-Cov-2 nucleic acid extraction yield through the device was 7.10×10^(6) copies/mL,while a commercial kit yielded 2.98×10^(6) copies/*** mean time to complete the entire assay,from SARS-CoV-2 nucleic acid extraction to amplification detection,was 36 min and 45 *** detection limit for SARS-CoV-2 nucleic acid was 250 copies/*** The integrated disposable devices may be used for SARS-CoV-2 Point-of-Care test(POCT).

关键词： An integrated disposable device SARS-Cov-2 Nucleic acid detection

Sixty Years of Printing and Printing Equipment Industries of China (Ⅰ)

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印刷工业 2009年第11期 84-87页

作者： Wang Demao ma xuejun

Ancient Chinese invented printing *** block printing appeared in Tang Dynasty in the beginning of the 7th century. Movable type

关键词：印刷技术中国发展历史木刻

Field Evaluation of a Duplex RT-RAA Assay for Rapid Detection of SARS-CoV-2--Hebei Province,China,January 2021

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China CDC weekly 2022年第23期4卷 504-508页

作者： Ruiqing Zhang Shunxiang Qi Jiandong Li Shiyou Liu Guangyue Han Xinxin Shen Qi Li Wenbo Xu xuejun ma National Institute for Viral Disease Control and Prevention China CDCBeijingChina Hebei Center for Disease Control and Prevention Shijiazhuang CityHebei ProvinceChina Center for Biosafety Mega-Science Chinese Academy of SciencesWuhan CityHubei ProvinceChina

Introduction:Recently,a local cluster epidemic has occurred in Shijiazhuang City,Hebei *** to promptly identify patients with fever in rural areas was the major reason for this ***:We presented the field evaluation of a new real-time reverse transcription recombinase-aided amplification(RT-RAA)kit incorporating an endogenous internal control in a single-tube format,completed at the Hebei CDC from January 17,2021 to January 27,***:We evaluated the diagnostic performance of RT-RAA assay using automatic extracted RNA of 808 clinical *** with reverse transcriptase real-time quantitative PCR(qRT-PCR),RT-RAA kit achieved 92.41%sensitivity,98.78%specificity and a 96.29%coincidence rate,demonstrating an excellent agreement between the RTRAA assay and qRT-PCR ***,58 samples were extracted using a manual extraction method within 5 minutes,but only samples with high nucleic acid concentration(cycle threshold value not higher than 32)could be stably ***:The RT-RAA is more suitable to meet the needs of rapid,sensitive,and accurate detection in community-level medical institutions.

关键词： Duplex Hebei prompt

Sixty Years of Printing and Printing Equipment Industries of China (Ⅱ)

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印刷工业 2009年第12期 94-97页

作者： Wang Demao ma xuejun

Printing technology and equipment industries developed quickly in market economy During the period of planned economy, the aim of China’s printing industry was meeting the need of do-

关键词：印刷技术数字印刷印刷行业市场

Development of a PDRA Method for Detection of the D614G Mutation in COVID-19 Virus—Worldwide,2021

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China CDC weekly 2021年第21期3卷 448-453页

作者： Ziwei Chen Xinxin Shen Ji Wang Xiang Zhao Yuan Gao Ruiqin Zhang Jinrong Wang Leping Liu Xinmin Nie xuejun ma Department of Laboratory Medicine Third Xiangya HospitalCentral South UniversityChangshaHunanChina Department of NHC Key Laboratory of Medical Virology and Viral Diseases National Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijingChina Department of Blood Transfusion Third Xiangya HospitalCentral South UniversityChangshaHunanChina Center for Biosafety Mega-Science Chinese Academy of SciencesWuhanHubeiChina.

ABSTRACT Background:COVID-19 infection is a major public health problem worldwide,and the D614G mutation enhances the infectivity of ***:A probe-directed recombinase amplification(PDRA)assay was discussed to detect the D614G mutation at 39℃for 30 *** sensitivity,specificity,and reproducibility of the PDRA were evaluated by D614 and G614 recombinant *** clinical performance of PDRA assay was validated by testing of 53 previously confirmed COVID-19 positive RNAs and 10 negative *** sequencing was carried out in parallel for ***:With good reproducibility and specificity,the PDRA assay worked well with the concentration in the range of 103–107 copies/*** with direct sequencing as a reference,the recombinase-aided amplification(RAA)assay obtained 100%sensitivity and 100%specificity using clinical samples.

关键词： specificity PDR D61

Development of an innovative one-step nested PCR strategy for virus detection using the LNA technique

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Science China(Life Sciences) 2019年第3期62卷 428-430页

作者： Ji Wang Li Zhao Zhihua Sun Guixia Li Peihua Niu DANDi Li Lianjun Wang Yi Zhang Zhishan Feng xuejun ma National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention Hebei Medical University Beijing Chaoyang maternal & Child Health Hospital Children's Hospital of Hebei Province Dongcheng Center for Disease Control and Prevention Beijing

Dear Editor,Polymerase chain reaction(PCR)is a molecular diagnostic technique that has been widely used for diagnosing viral *** PCR(N-PCR)is a variation on the standard PCR technique that involves two amplification steps,yielding greater sensitivity and *** there are two main disadvantages:the protocol is more complex than conventional PCR,and the risk of cross-contamination is relatively ***-step nested PCR(OSN-PCR)enables conventional N-PCR to be performed as a closed reaction in a single tube that contains both outer and inner *** strategy makes OSN-PCR more rapid than N-PCR,lowers the probability of cross-contamination,and requires a smaller amount of reagents.

关键词： Dear Editor PCR strategy LNA technique

A Novel Coronavirus Genome Identified in a Cluster of Pneumonia Cases--Wuhan,China 2019−2020

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China CDC weekly 2020年第4期2卷 61-62页

作者： Wenjie Tan Xiang Zhao xuejun ma Wenling Wang Peihua Niu Wenbo Xu George F.Gao Guizhen Wu MHC Key Laboratory of Biosafety National Institute for Viral Disease Control and PreventionChina CDCBeijingChina Center for Biosafety Mega-Science Chinese Academy of SciencesBeijingChina.

Emerging and re-emerging pathogens are great challenges to the public health(1).A cluster of pneumonia cases with an unknown cause occurred in Wuhan starting on December 21,*** of January 20,2020,a total of 201 cases of pneumonia in China have been confirmed.A team of professionals from the National Health Commission and China CDC conducted epidemiological and etiological *** January 3,2020,the first complete genome of the novelβgenus coronaviruses(2019-nCoVs)was identified in samples of bronchoalveolar lavage fluid(BALF)from a patient from Wuhan by scientists of the National Institute of Viral Disease Control and Prevention(IVDC)through a combination of Sanger sequencing,Illumina sequencing,and nanopore *** distinct strains have been identified,the virus has been designated as 2019-nCoV,and the disease has been subsequently named novel coronavirus-infected pneumonia(NCIP).

关键词： lavage BALF Wuhan

Enhancement of a recombinase-aided amplification assay using betaine and pullulan

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Infectious Medicine 2022年第2期1卷 73-80页

作者： Jinrong Wang Guowei Song Yue Ming Jing Pan Ruiqing Zhang Guohao Fan Xinxin Shen xuejun ma Lixin Li Shijiazhuang People’s Hospital ShijiazhuangHebei050051China Qiqihar Medical University QiqiharHeilongjiang161000China NHC Key Laboratory of Medical Virology and Viral Diseases National Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing102206China

Background:Nucleic acid amplification enhancers suitable for use in a recombinase-aided amplification(RAA)assay were studied for the first time,and amplification of a long-fragment(509 bp)was initially ***:Using recombinant plasmids and clinical samples,RAA fluorescence and basic methods were used to evaluate the *** fluorescence method was evaluated by threshold time and fluorescence value,and the basic method was characterized by 2%agarose gel ***:Taking a previously established RAA assay for HPV18 as an example,we demonstrated that the addition of 0.2 M,0.4 M,and 0.6 M betaine and 10%pullulan could enhance the *** new RAA assays with betaine and pullulan were named B-RAA and P-RAA,*** the B-RAA and P-RAA fluorescence methods,the threshold time values could be shortened by 1.72-2.32 minutes and 2.60 minutes,respectively,and the fluorescence values could be enhanced by 8847.25-9094.37 mv and 5250 mv,*** the basic method,the sensitivity could be increased *** successfully amplified a long-fragment of 509 bp using a P-RAA assay with a sensitivity of 102 copies/μL(compared with 103 copies/μL in the RAA assay).Conclusions:Thus,we concluded that betaine and pullulan are effective additives to enhance the sensitivity of RAA assays.

关键词： Nucleic acid amplification enhancer Betaine Pullulan RAA Long-fragment

在城市与乡村之间:李大钊“物心两面”的社会改造观

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中国农业大学学报（社会科学版） 2019年第4期36卷 5-12页

作者：马学军中国农业大学人文与发展学院社会学与人类学系

李大钊是中共第一个马克思主义者,他主张"灵肉一致"和"物心两面"的社会改造观。早期他受托尔斯泰人道主义和泛劳动主义的影响,主张道德革命和精神改造,号召青年发扬劳动主义,到民间去与农民为伍。在接受马克思主义之后,李大钊也主张物... 详细信息

李大钊是中共第一个马克思主义者,他主张"灵肉一致"和"物心两面"的社会改造观。早期他受托尔斯泰人道主义和泛劳动主义的影响,主张道德革命和精神改造,号召青年发扬劳动主义,到民间去与农民为伍。在接受马克思主义之后,李大钊也主张物质改造运动,但他认为物质改造和阶级斗争仅是手段,最终还是要达到互助和人道主义的精神目标。李大钊后来虽也提出"工人政治"的表述,也领导了中共的工人运动,但随着都市工人运动的失败,他较早提出中国革命应依靠农民、应到农村开展农村革命。深入研究李大钊的思想,对我们理解以无产阶级为基础的中共,从城市革命转向农村革命,具有重要的理论意义。

关键词：李大钊道德革命物质改造社会改造到农村去