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Universal and one-step visualization of latent fingermarks onvarious surfaces using hydrophilic cellulose membrane and dye aqueous solution

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Science China Chemistry 2017年第9期60卷 1250-1257页

作者： Qianhui Wei Xiaolong Li Xin Du xueji zhang Meiqin zhang Research Center for Bioengineering&Sensing Technology University of Science&Technology BeoingBeijing 100083China

A high-resolution and universal method for the rapid visualization of latent fingermarks on a broad variety of surfaces has been achieved by simply combining hydrophilic cellulose membrane with dye aqueous solution. In this approach, the relatively hydrophobic characteristic of fingermark residue enable the deposit act as a “mask”, directing dye absorption processes to regions of both furrows without the ridge residues and bare cellulose membrane substrate. This effect during the spatially selective dye absorption generates a negative pattern of the fingermark within a few seconds to minutes depending on the properties of the substrates and fingermark types. It provides observation of latent fingermarks on cellulose membrane surface with high definition of level 2 and level 3 details used for personal identification purposes. It is also highly-efficient for visualizing the latent fingermarks lifted from various common (glass, carton, ceramic cup) and problematic (banknote, human skin and leather) surfaces by using transparent adhesive tape. To examine the generality of the proposed method, eight different kinds of dye aqueous solutions have been tested for developing latent fingermarks and they all provide very good results as expected. Moreover, this approach is easily used for aged fingermarks and natural fingermarks as well. Imaging may be accomplished visibly (by dye color) and topographically (using a microscope or a camera). This approach is a simple, rapid, non-destructive, safe, low-cost, universal and high resolution method compared with conventional approaches, so it demonstrates good potentiality in individual identity validation related applications. © 2017, Science China Press and Springer-Verlag Berlin Heidelberg.

关键词： Cellulose Hydrophilicity Membranes Substrates Visualization

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Emerging open-channel droplet arrays for biosensing

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National Science Review 2023年第10期10卷 172-186页

作者： Yongchao Song Lirong Wang Tailin Xu Guangyao zhang xueji zhang School of Biomedical Engineering Health Science CenterShenzhen University Intelligent Wearable Engineering Research Center of Qingdao Research Center for Intelligent and Wearable Technology College of Textiles and ClothingState Key Laboratory of Bio-Fibers and Eco-Textiles Qingdao University

Open-channel droplet arrays have attracted much attention in the fields of biochemical analysis,biofluid monitoring,biomarker recognition and cell interactions,as they have advantages with regard to miniaturization,parallelization,high-throughput,simplicity and *** droplet arrays not only improve the sensitivity and accuracy of a biosensor,but also do not require sophisticated equipment or tedious processes,showing great potential in next-generation miniaturized sensing *** review summarizes typical examples of open-channel micro droplet arrays and focuses on diversified biosensing integrated with multiple signal-output approaches(fluorescence,colorimetric,surface-enhanced Raman scattering(SERS),electrochemical,etc.).The limitations and development prospects of open-channel droplet arrays in biosensing are also discussed with regard to the increasing demand for biosensors.

关键词： open-channel droplet wettable pattern mini-pillar platform liquid marbles multiple biosensing

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Photoluminescent two-dimensional SiC quantum dots for cellular imaging and transport

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Nano Research 2018年第8期11卷 4074-4081页

作者： Yu Cao Haifeng Dong Shaotao Pu xueji zhang Research Center for Bioengineering and Sensing Technology Beijing Key Laboratory for Bioengineering and Sensing TechnologSchool of Chemistry & Biological Engineering University of Science & Technology Beijing Beijing 100083 China

Two-dimensional （2D） ultrathin SiC has received intense attention due to its broad band gap and resistance to large mechanical deformation and external chemical corrosion. However, the synthesis and application of ultrasmall 2D SiC quantum dots （QDs） has not been explored. Herein, we synthesize a type of monolayered 2D SiC QDs with advanced photoluminescence （PL） properties via a facile hydrothermal route. Their average size and thickness can be easily adjusted by altering the reaction time. The ultrasmall 2D SiC QDs exhibit a long fluorescence lifetime of 2.59 ps due to efficient quantum confinement. The applications of SiC QDs are demonstrated through labeling A549, HeLa, and NHDF cells and delivering agents for intracellular low-abundant microRNA （miRNA） detection. This advance in preparing photoluminescent SiC QDs is of great significance for broadening their potential in biomedical and optical applications.

关键词： two-dimensional SiC quantum dots cellular imaging intraceUular microRNA(miRNA) detection

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Optogenetic Control of Phosphatidylinositol (3,4,5)-Triphosphate Production by Light-Sensitive Cryptochrome Proteins on the Plasma Membrane

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Chinese Journal of Chemistry 2021年第5期39卷 1240-1246页

作者： Lingzhi Yang Takeaki Ozawa Haifeng Dong xueji zhang School of Pharmaceutical Sciences Health Science CenterShenzhen UniversityShenzhenGuangdong 518060China Deportment of Chemistry School of ScienceThe University of Tokyo7-3-1 HongoBunkyo-kuTokyo 113-0033Japan School of Biomedical Engineering Health Science CenterShenzhen UniversityShenzhenGuangdong 518060China

Phosphatidylinositol(3,4,5)-triphosphate(PIP3),acting as a fundamental second messenger,is emerging as a promising biomarker for disease diagnosis and ***,the real time analysis of phosphoinositide in living cells remains a key challenge owing to the low basal abundance and its fast metabolic ***,we design an optogenetic system that uses light sensitive protein-protein interaction between Arabidopsis cryptochrome 2(CRY2)and CIB1 to spatiotemporally visualize the PIP3 production with sub-second *** this system,a CIBN is anchored on the plasma membrane,whereas a CRY2 fused with a constitutively active PI3-kinase(acPI3K)would be driven from the cytosol to the membrane by the blue-light-activated CRY2-CIB1 interaction upon light *** PIP3 production is visualized via a fused fluorescent protein by the translocation of a Pleckstrin Homology(PH)domain(GRP1)from the cytosol to the plasma membrane with high *** demonstrated the fast dynamics and reversibility of the optogenetic system initiated PIP3 synthesis on the plasma ***,the real-time cell movements were also observed upon localized light *** established optogenetic method provides a novel spatiotemporal strategy for specific PIP3 visualization,which is beneficial to improve the understanding of PIP3 functions.

关键词： Protein-protein interactions Protein engineering Fluorescent probes Optogenetic control Phosphatidylinosito (3,4,5)-triphosphate

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Near-infrared light-driven yolk@shell carbon@silica nanomotors for fuel-free triglyceride degradation

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Nano Research 2021年第3期14卷 654-659页

作者： Yi Xing Songsong Tang Xin Du Tailin Xu xueji zhang Research Center for Bioengineering and Sensing Technology Beijing Key Laboratory for Bioengineering and Sensing TechnologyDepartment of Chemistry&Biological EngineeringUniversity of Science&Technology BeijingBeijing100083China

Yolk@shell mesoporous nanoparticles have received close attention due to their controllable structures and integrated ***,most yolk@shell nanosystems lack ***,yolk@spiky-shell structured carbon@silica nanomotors are fabricated with near-infrared(NIR)light self-thermophoretic propulsion as lipase nanocarriers for fuel-free triglyceride *** light propulsion accelerates the accumulation of nanomotors on the droplet interface,and the efficient lipase loading further facilitates the rapid degradation of tributyrin *** adjusting the yolk and spiky structure,the obtained semi-yolk@spiky-shell structured nanomotors exhibit the highest capacity of lipase(442 mg/g)and the highest light-driven diffusion coefficient(ca.55%increase under 2 W/cm^(2 )irradiation),thus improving the degradation efficiency of triglyceride(93.1%under NIR light vs.66.7%without NIR light within 20 min).This work paves the way to rationally design yolk@shell structured nanomotors for diverse applications.

关键词： yolk@spiky-shell carbon@silica nanomotors self-thermophoresis triglyceride degradations

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Latent fingerprint enhancement on conductive substrates using electrodeposition of copper

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Science China Chemistry 2015年第7期58卷 1200-1205页

作者： Meiqin zhang Xi Yu Gang Qin Yu Zhu Meiling Wang Qianhui Wei Yang zhang xueji zhang Research Center for Bioengineering and Sensing Technology University of Science and Technology Beijing

We presented a novel method for the development of a latent fingerprint by selective electrodeposition of a copper thin film from sulfate solution onto the conductive substrate between fingerprint ridges to generate a negative image of the fingerprint deposit. After optimizing the parameters(deposition time, deposition potential, and copper concentration), the preferential electrodeposition of copper films allowed latent fingerprints on six kinds of conductive surfaces(indium/tin oxide-coated glass, silver sheet, platinum sheet, gold sheet, copper sheet, and a stainless steel coin) to be successfully developed with high resolution. In addition, this technique could also be exploited to visualize latent fingerprints on rough and dirty surfaces. The quality of the developed fingerprints was estimated visually and the morphology of the copper film was characterized by field emission scanning electron microscopy.

关键词： latent fingerprint visualization electrodeposition copper thin film forensic science electrochemistry

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Rapid detection of high-risk HPV16 and HPV18 based on microchip electrophoresis

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Journal of Pharmaceutical Analysis 2020年第4期10卷 329-333页

作者： Zhaoxuan Fan Xiao Feng Weifei zhang xueji zhang Jin-Ming Lin Research Center for Bioengineering and Sensing Technology School of Chemistry and Biological EngineeringUniversity of Science and Technology BeijingBeijing100083PR China Department of Chemistry Beijing Key Laboratory of Microanalytical Methods and InstrumentationMOE Key Laboratory of Bioorganic Phosphorus Chemistry&Chemical BiologyTsinghua UniversityBeijing100084PR China State Key Laboratory of Chemical Resource Engineering Beijing University of Chemical TechnologyBeijing100029PR China School of Biomedical Engineering Shenzhen UniversityShenzhenGuangdong 518060PR China

Researches on detection of human papillomavirus(HPV)high-risk samples were carried out by polymerase chain reaction(PCR)coupled with microchip electrophoresis(MCE).Herein,we introduced a simple,rapid,automated method for detecting high-risk samples HPV16 and *** this research,general primers were initially selected to obtain sufficient detectable yield by PCR to verify feasibility of MCM method for HPV detection,then type-specific primers were further used to evaluate the specificity of MCE *** results indicated MCE method was capable of specifically detecting high-risk HPV16 and HPV18,and also enabled simultaneous detection of multiplex *** MCE method described here has been successfully applied to HPV detection and displayed excellent reliability demonstrating by sequencing *** inherent capability of MCE facilitated HPV detection conducted in a small chip with automated,high throughput,massive parallelized *** envision that MCE method will definitely pave a way for clinical diagnosis,and even on-site screening of cervical cancer.

关键词： Human papillomavirus DNA analysis Polymerase chain reaction Microchip electrophoresis Detection

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Oxidation and detection of L-cysteine using a modified Au/Nafion/glass carbon electrode

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Science China Chemistry 2011年第3期54卷 521-525页

作者： WANG xueji zhang LiLi MIAO LiXiao KAN MeiXiu KONG LinLin zhang HuiMin School of Science Beijing Institute of Technology Beijing 100081 China

In this work,the electrochemical oxidation of L-cysteine(CySH)was investigated on a composite film modified electrode with Au nanoparticles dispersed in the fluorocarbon polymer(Nafion).The excellent electrocatalytic effect on CySH oxidation was attributed to the role of Au *** voltammetric studies revealed two anodic peaks for the oxidation of CySH in the pH range of 2.0–*** electrode was used to detect cysteine at pH 2.0 and pH *** pH 2.0,a determination range of 3.0–50.0?mol/L was obtained with the detection sensitivity of 22.7A/(mmol L1),while at pH 7.0,a determination range of 2.0–80.0mol/L was obtained with the detection sensitivity of 4.08A/(mmol L1).The detection limits were estimated to be as low as 1.0?mol/L at both pH 7.0 and pH ***,at pH 7.0,the interferences of ascorbic acid and uric acid were reduced for the detection of *** made the Au/Nafion/GC electrode a promising candidate for efficient electrochemical sensors for the detection of CySH.

关键词： L-cysteine composite film gold nanoparticles Nafion detection

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Flexible microfluidic nanoplasmonic sensors for refreshable and portable recognition of sweat biochemical fingerprint

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npj Flexible Electronics 2022年第1期6卷 593-602页

作者： Xuecheng He Chuan Fan Yong Luo Tailin Xu xueji zhang School of Biomedical Engineering Health Science CenterShenzhen UniversityShenzhen 518060People’s Republic of China Beijing Advanced Innovation Center for Materials Genome Engineering Research Center for Bioengineering and Sensing TechnologyUniversity of Science and Technology BeijingBeijing 100083People’s Republic of China

Wearable sweat sensors with various sensing systems can provide noninvasive medical diagnostics and healthcare ***,we demonstrate a wearable microfluidic nanoplasmonic sensor capable of refreshable and portable recognition fingerprint information of targeted biomarkers including urea,lactate,and pH in sweat.A miniature,thin plasmonic metasurface with homogeneous mushroom-shaped hot spots and high surface-enhanced Raman scattering(SERS)activity is designed and integrated into a microfluidics *** to conventional wearable SERS platforms with the risk of mixed effect between new and old sweat,the microfluidic SERS system allows sweat administration in a controllable and high temporal-resolution fashion,providing refreshable SERS *** use a portable and customized Raman analyzer with a friendly human-machine interface for portable recognition of the spectroscopic signatures of sweat *** study integrates epidermal microfluidics with portable SERS molecular recognition,presenting a controllable,handy,and dynamical biofluid sensing system for personalized medicine.

关键词： signature portable fluid

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Artificial intelligence biosensors for continuous glucose monitoring

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Interdisciplinary Materials 2023年第2期2卷 290-307页

作者： Xiaofeng Jin Andrew Cai Tailin Xu xueji zhang School of Life Sciences Anhui UniversityHefeiChina McKelvey School of Engineering Washington University in St.LouisSt.LouisMissouriUSA School of Biomedical Engineering Shenzhen UniversityShenzhenChina

Artificial intelligence(AI)algorithms in combination with continuous monitoring technologies have the potential to revolutionize chronic disease *** recent innovations in both continuous glucose monitoring(CGM)and the closed-loop highlight the far-reaching potential of AI biosensors for individual *** review summarizes some of the most advanced progress made in CGM *** will focus on three main applications of AI algorithms in diabetes management:closed-loop control algorithms,glucose predictions,and *** challenges and opportunities of AI technologies for CGM in individualized and proactive medicine will also be discussed.

关键词： artificial intelligence closed-loop continuous glucose monitoring machine learning

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