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Journal of Integrative Agriculture 2024年第3期23卷 849-862页

作者： Jiawei Pan Jia Song Rahat Sharif Xuewen Xu Shutong Li xuehao chen School of Horticulture and Landscape Architecture Yangzhou UniversityYangzhou 225009China Institute of Vegetables Tianjin Academy of Agricultural SciencesTianjin 300384China

Leaf color mutants in higher plants are considered to be ideal materials for studying the chlorophyll biosynthesis,photosynthesis mechanism and chloroplast ***,we identified a spontaneous mutant,yc412,in cultivated cucumber that exhibited yellow *** yellow-lethal mutant was diagnosed with an abnormal chloroplast ultrastructure,and reduced photosynthetic capacity and pigment *** bulked segregant analysis-based whole-genome sequencing and fine genetic mapping,we narrowed the yellow cotyledons (yc) locus to a 96.8 kb interval on chromosome *** resequencing and molecular cloning,we showed that Csyc is a potential candidate gene,which encodes a yellow stripe-like (YSL) *** T to C mutation in the promoter region of Csyc caused the yellow cotyledon phenotype in *** to YZU027A (WT),the expression of Csyc was significantly downregulated in the cotyledons of *** of Csyc in cucumber via virus-induced gene silencing resulted in chlorotic leaves,mainly by suppressing the chlorophyll ***,a comparative transcriptome analysis revealed that chloroplast-related genes and chlorophyll biosynthesis genes were significantly downregulated in yc412 *** results provide new insights into the molecular function of the YSL transporter in plant chloroplast development and chlorophyll synthesis.

关键词： cucumber yellow-lethal cotyledons chloroplast development yellow stripe-like transporter

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蔬菜作物盐胁迫响应及耐盐机制研究进展

中国蔬菜 2024年第4期 23-33页

作者：陈嵘峰屠静韵许学文陈学好上海农场生活服务有限公司江苏盐城224100 扬州大学园艺园林学院江苏扬州225009

近年来,由于不合理的灌溉及其他环境因素,土壤盐渍化问题日趋严重,盐胁迫严重影响蔬菜作物生长、发育和产量,限制土地利用。盐胁迫主要改变蔬菜作物的净光合生产率、呼吸作用、细胞膜结构和各类酶活性。本文综述了盐胁迫对蔬菜作物表观... 详细信息

近年来,由于不合理的灌溉及其他环境因素,土壤盐渍化问题日趋严重,盐胁迫严重影响蔬菜作物生长、发育和产量,限制土地利用。盐胁迫主要改变蔬菜作物的净光合生产率、呼吸作用、细胞膜结构和各类酶活性。本文综述了盐胁迫对蔬菜作物表观形态的改变和植物内部生理生化指标的影响,阐述了蔬菜对盐胁迫的生理响应(包括渗透调节、离子区域化、抗氧化代谢)和分子调控(包括转录因子、离子平衡调控基因、抗氧化基因、小RNA)机制;总结了各类蔬菜耐盐性的鉴定方法;展望了蔬菜作物耐盐性的研究方向。

关键词：蔬菜作物盐胁迫耐盐机制表型鉴定

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大蒜、青菜等蔬菜作物对根际微生物的影响

扬州大学学报（农业与生命科学版） 2024年第1期45卷 50-58页

作者：徐浴力周昕陈学好徐强扬州大学园艺园林学院江苏扬州225009

大蒜和青菜是重要的蔬菜作物,对土壤微生物群落有着不同的影响。通过Illumina MiSeq测序技术,研究大蒜、青菜等不同蔬菜作物对根际微生物的影响。结果表明:Q (株行距0.1 m×0.3 m,种植青菜)处理土壤细菌群落的多样性和丰度增加,S (株行... 详细信息

大蒜和青菜是重要的蔬菜作物,对土壤微生物群落有着不同的影响。通过Illumina MiSeq测序技术,研究大蒜、青菜等不同蔬菜作物对根际微生物的影响。结果表明:Q (株行距0.1 m×0.3 m,种植青菜)处理土壤细菌群落的多样性和丰度增加,S (株行距0.1 m×0.3 m,种植大蒜)和SC (株行距0.2 m×0.3 m,种植大蒜)处理土壤细菌群落多样性和丰度减小;S和SC处理对细菌β多样性的影响高于Q处理;芽单胞菌属(Gemmatimonas)、芽孢杆菌属(Bacillus)为S、SC处理共有的优势菌属,相对丰度显著高于Q处理和CK;芽单胞菌属与Saccharibacteria、Ramlibacter等菌属呈正相关,与黄色类固醇杆菌属(Steroidobacter)、JTB255等菌属呈负相关,对细菌群落构成有重要影响,降低大蒜种植密度可降低芽单胞菌属的相对丰度,合理的大蒜种植密度可起到改善细菌群落结构的作用。综上,该研究对进一步了解蔬菜作物根系微生物群落的作用、活动调控机制以及土传病害与连作障碍的关系具有重要意义。

关键词：大蒜青菜根际土壤 Illumina高通量测序细菌群落芽单胞菌

后冬奥时期大众滑雪运动发展的现实困境及纾解之策——以长春市为例

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冰雪体育创新研究 2024年第4期 4-6页

作者：陈学豪张格安徽师范大学体育学院安徽芜湖241003

在2015年申奥成功后,人们将目光转向了大众滑雪,滑雪运动从以前的"贵族"运动,转向了"大众"运动,成为一项人人渴望参与,人人积极参与的群众性运动。2022北京冬奥会的成功举办,将滑雪运动再次推向高潮,为保障后冬奥时期大众滑雪的可持续发... 详细信息

在2015年申奥成功后,人们将目光转向了大众滑雪,滑雪运动从以前的"贵族"运动,转向了"大众"运动,成为一项人人渴望参与,人人积极参与的群众性运动。2022北京冬奥会的成功举办,将滑雪运动再次推向高潮,为保障后冬奥时期大众滑雪的可持续发展,该文以长春市三家滑雪场的滑雪者参与情况为例,通过文献资料法、田野调查法等方法,分析大众滑雪运动发展过程中面临的现实困境,提供相应的纾解之策,为群众性冰雪运动发展提供理论支撑。

关键词：后冬奥时期冰雪运动大众滑雪

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黄瓜体内营养物质对瓜蚜抗生性的影响

扬州大学学报（农业与生命科学版） 2024年第1期45卷 133-138页

作者：夏彭飞任佳韩杜斌许学文陈申周福才陈学好南京新创蔬菜分子育种研究院有限公司南京211899 扬州大学植物保护学院江苏扬州225009 扬州大学园艺园林学院江苏扬州225009

瓜蚜是黄瓜上重要害虫,严重影响黄瓜产量和品质,筛选和利用抗蚜品种是减轻黄瓜蚜虫为害的有效手段。以瓜蚜不同抗性的13个黄瓜品种为材料,研究黄瓜叶片可溶性蛋白质、氨基酸及可溶性糖3种营养物质含量与抗蚜性关系,以建立快速鉴定黄瓜... 详细信息

瓜蚜是黄瓜上重要害虫,严重影响黄瓜产量和品质,筛选和利用抗蚜品种是减轻黄瓜蚜虫为害的有效手段。以瓜蚜不同抗性的13个黄瓜品种为材料,研究黄瓜叶片可溶性蛋白质、氨基酸及可溶性糖3种营养物质含量与抗蚜性关系,以建立快速鉴定黄瓜品种抗蚜性的方法。结果表明:黄瓜叶片可溶性糖、游离脯氨酸含量与瓜蚜数量呈显著正相关,其回归方程分别为Y=0.0002X+0.0371(r=0.6195,P=0.0021)和Y=0.5620X+34.0324(r=0.7423,P=0.0251);叶片可溶性蛋白质含量与叶片瓜蚜种群数量无显著相关性。对可溶性糖(X_(1))和游离脯氨酸(X_(2))进行逐步回归分析,其回归方程为Y=-69.6650+1294.7471X_(1)+0.8678X_(2)(R^(2)=0.8036^(*))。黄瓜营养物质中可溶性糖和游离脯氨酸对瓜蚜的抗性具有重要作用,可作为鉴定黄瓜抗蚜性的指标。

关键词：黄瓜瓜蚜抗虫性抗生性

Cucumber glossy fruit 1 (CsGLF1) encodes the zinc finger protein 6 that regulates fruit glossiness by enhancing cuticular wax biosynthesis

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Horticulture Research 2023年第1期10卷 152-159页

作者： Yan Yang Congxi Cai Yipeng Wang Yanran Wang Haolun Ju xuehao chen School of Horticulture and Plant Protection Yangzhou UniversityYangzhou225009Jiangsu ProvinceChina

Cucumber glossiness is an important visual quality trait that affects consumer *** evidence suggests that glossy trait is associated with cuticular wax ***,the molecular genetic mechanism controlling cucumber glossiness remains largely ***,we report the map-based cloning and functional characterization of CsGLF1,a locus that determines the glossy trait in ***1 encodes a homolog of the Cys_(2)His_(2)-like fold group(C2H2)-type zinc finger protein 6(ZFP6)and its deletion leads to glossier pericarp and decreased cuticular wax ***,transcriptomic analysis demonstrated that a group of wax biosynthetic genes were downregulated when CsZFP6 was ***,transient expression assay revealed that CsZFP6 acted as a transcription activator of cuticular wax biosynthetic *** together,our findings demonstrated a novel regulator of fruit glossiness,which will provide new insights into regulatory mechanism of fruit glossiness in cucumber.

关键词： gloss fruit mechanism

欧美篮球强国青少年人才培养体系的特点与启示

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中国体育教练员 2024年第2期32卷 30-34页

作者：陈学豪薛昭铭穆范正蔡维敏安徽师范大学安徽芜湖241002 南通大学江苏南通226019

分析欧美篮球强国青少年篮球培养体系,借鉴欧洲以俱乐部青训为主和美国以校园篮球为主的青少年人才培养模式,发现其存在赛事体系完善、上升通道清晰、学训平衡、管理有序和关注度高等优势。结合我国青少年篮球人才培养体系存在的赛事少... 详细信息

分析欧美篮球强国青少年篮球培养体系,借鉴欧洲以俱乐部青训为主和美国以校园篮球为主的青少年人才培养模式,发现其存在赛事体系完善、上升通道清晰、学训平衡、管理有序和关注度高等优势。结合我国青少年篮球人才培养体系存在的赛事少、流动性差、地区发展不平衡、学训矛盾、管理混乱和关注度低等问题,提出深化体教融合、完善青少年赛事体系、发挥好社会力量、加强管理等建议。

关键词：欧美国家青少年篮球培养体系后备人才体教融合

Identification of Genes Related to Mesocarp Development in Cucumber

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Horticultural Plant Journal 2020年第5期6卷 293-300页

作者： Xiaohua Qi Yamei Zhu Shuifeng Li Hanxi Zhou Xuewen Xu Qiang Xu xuehao chen College of Horticulture and Plant Protection Yangzhou UniversityYangzhou225009China Xiaoshan Agricultural Technology Extension Center Hangzhou 311200China

Cucumber(Cucumis sativus L.)is an agriculturally and economically important vegetable crop *** thickness of mesocarp is an important quality pertaining to *** development of mesocarp,however,has received little *** this study,we selected one thick mesocarp line D8 to analyze the gene expression profiles of mesocarp at 0,3,6,and 9 days after pollination(DAP)by RNA *** mainly related to ribosomes,carbon metabolism,biosynthesis of amino acids,and oxidative phosphorylation were up-regulated at 3 *** 3 to 6 DAP,the genes related to oxidative phosphorylation,photosynthesis,and phagosome were highly ***6 to 9 DAP,the genes related to plant hormone signal transduction were highly *** expressed genes linked to cyclin,hormone biosynthesis and signaling,carbon metabolism,and transcription factors MYB and WRKY were common up-regulated at 3,6,9 DAP in comparison with 0 *** auxin-responsive and cytokinin-responsive genes were significantly up-regulated in mesocarp *** higher expression levels of the auxin-responsive gene indole-3-acetic acid-amido synthetase 3.6 and the cytokinin response regulator 3/4b in the thick mesocarp cultivar D8 than in the thin mesocarp cultivar XUE1 might have explained the different growth rates of the mesocarp.

关键词： cucumber mesocarp auxin cytokinin RNA-seq

Screening and identification of candidate Fusarium wilt-resistance genes from pumpkin

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Horticultural Plant Journal 2022年第5期8卷 583-592页

作者： Jun Xu Qianqian Xian Ke Wang Jingping Dong Chaoyang Zhang Shengli Du Xuewen Xu xuehao chen School of Horticulture and Plant Protection Yangzhou UniversityYangzhouJiangsu 225009China Huai’an Key Laboratory for Agricultural Biotechnology Huaiyin Institute of Agricultural Sciences in Xuhuai Region of Jiangsu ProvinceHuai’anJiangsu 223001China State Key Laboratory of Vegetable Germplasm Innovation Tianjin 300192China

Pumpkin(Cucurbita moschata)has been widely used as cucumber(Cucumis sativus L.)rootstock to defend against Fusarium wilt(FW)and increase cucumber yields and ***,the resistance genes and mechanisms underlying the FW tolerance in pumpkin are poorly *** we analyzed the transcriptome of pumpkin inoculated with the cucumber FW causal agent Fusarium oxysporum ***(Foc),and obtained 3152 and 4735 upregulated genes induced by Foc at 24h after Foc inoculation compared with at 0h and 24h non-inoculated control,***,404 common differentially expressed genes(DEGs)were screened using the criterion log_(2) FPKM(fold change)≥*** total,206 of 404 DEGs were predominantly expressed in roots,which is the first tissue that Foc contacts and invades.140 DEGs were selected and classified into four groups(pathogenesis resistance,secondary metabolism-related,transcription factor and signal binding)based on their functional ***,29 genes having high expression levels were selected to investigate the expression patterns induced by a Foc *** them,16 genes were significantly induced by Foc and showed high expression levels at various treatment time *** candidate genes may act as positive regulators of FW resistance in pumpkin and provide effective resources for improving cucumber FW resistance through breeding programs.

关键词： Pumpkin Rootstock Transcriptome Fusarium wilt Resistance genes

Insights into flavonoid biosynthesis during cucumber fruit peel coloration based on metabolite profiling and transcriptome analyses

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Horticultural Plant Journal 2023年第4期9卷 763-776页

作者： Shuying He Yi Ye Ying Yuan Mai Lv Meixing Wang Qiang Xu Xuewen Xu xuehao chen School of Horticulture and Landscape Architecture Yangzhou UniversityYangzhouJiangsu 225009China Joint International Research Laboratory of Agriculture and Agri-Product Safety The Ministry of Education of ChinaYangzhou UniversityYangzhouJiangsu 225009China

The fruit peel color is a crucial trait of *** better understand the molecular mechanisms underlying cucumber peel coloration,we compared the UPLC-ESI-MS/MS-based flavonoid metabolomic and RNA sequencing-based transcriptomic profiling of the brown peeled cucumber line‘PW’at six developmental stages.A total of 210 flavonoid metabolites were *** which,117 flavonoid metabolites were differentially *** this study,weighted gene co-expression network analysis combined with Kyoto Encyclopedia of Gene and Genomes enrichment analysis revealed key genes coding for seven enzymes and eight transcription factors(TFs)associated with flavonoid *** them,the R2R3MYB CsaV3_4G001130 is the best candidate gene that is responsible for controlling mature fruit colors in *** sequencing revealed one nonsynonymous SNP in the exon of CsaV3_4G001130 among the selected 11 cucumber lines,which introduced a premature stop codon,generating a truncated protein in pale yellow or creamy peeled *** two-hybrid assays showed a direct interaction of CsaV3_4G001130 with the bHLH TF CsaV3_1G002260 and the WD40 protein CsaV3_***,the interactions were influenced by the nonsynonymous SNP we *** finding revealed that the integrated transcriptome and metabolome analysis further demonstrated that the abundance of some pigmented flavonoids(especially anthocyanins and chalcones)contributed to the coloration of‘PW’*** findings pave the way for elucidation of flavonoid biosynthesis and improvement of cucumber peel color in the future.

关键词： Cucumber Fruit peel coloration Transcriptome Metabolome MYB