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A cell transcriptomic profile p ovides insights into adipocytes of porcine mammary gland across development

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Journal of Animal Science and Biotechnology 2024年第1期15卷 182-200页

作者： Yongliang Fan Long Jin Zhiping He Tiantian Wei Tingting Luo Jiaman Zhang Can Liu Changjiu Dai Chao A Yan Liang Xuan Tao xuebin lv Yiren Gu Mingzhou Li State Key Laboratory of Swine and Poultry Breeding Industry College of Animal Science and TechnologySichuan Agricultural UniversityChengdu 611130China Key Laboratory of Qinghai‑Tibetan Plateau Animal Genetic Resource Reservation and Utilization Southwest Minzu UniversityChengdu 610041China Animal Breeding and Genetics Key Laboratory of Sichuan Province Sichuan Animal Science AcademyChengdu 610000China

Background Studying the composition and developmental mechanisms in mammary gland is crucial for healthy growth of newborns. The mammary gland is inherently heterogeneous, and its physiological function dependents on the gene expression of multiple cell types. Most studies focused on epithelial cells, disregarding the role of neighboring *** Here, we constructed the largest transcriptomic dataset of porcine mammary gland cells thus far. The dataset captured 126,829 high-quality nuclei from physiological mammary glands across five developmental stages(d 90 of gestation, G90;d 0 after lactation, L0;d 20 after lactation, L20;2 d post natural involution, PI2;7 d post natural involution, PI7). Seven cell types were identified, including epithelial cells, adipocytes, endothelial cells, fibroblasts cells, immune cells, myoepithelial cells and precursor cells. Our data indicate that mammary glands at different developmental stages have distinct phenotypic and transcriptional signatures. During late gestation(G90), the differentiation and proliferation of adipocytes were inhibited. Meanwhile, partly epithelial cells were completely differentiated. Pseudo-time analysis showed that epithelial cells undergo three stages to achieve lactation, including cellular differentiation, hormone sensing, and metabolic activation. During lactation(L0 and L20), adipocytes area accounts for less than 0.5% of mammary glands. To maintain their own survival, the adipocyte exhibited a poorly differentiated state and a proliferative capacity. Epithelial cells initiate lactation upon hormonal stimulation. After fulfilling lactation mission, their undergo physiological death under high intensity lactation. Interestingly, the physiological dead cells seem to be actively cleared by immune cells via CCL21-ACKR4 pathway. This biological process may be an important mechanism for maintaining homeostasis of the mammary gland. During natural involution(PI2 and PI7), epithelial cell populatio

关键词： Adipocytes Cell–cell interaction Development Mammary gland snRNA-seq

Study on PRRSV Receptor Genes Differential Expression in Lung Tissues in Different Breeds of Pigs after Infecting with HP-PRRSV

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Animal Husbandry and Feed Science 2017年第4期9卷 229-233,258页

作者： Kang Runmin Ji Gaosheng Zeng Kai lv xuebin Yin Mingyu Animal Breeding and Genetics Key Laboratory of Sichuan Province Sichuan Animal Science Academy Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province Key Laboratory of Bio-resources and Ecoenvironment of Ministry of EducationCollege of Life SciencesSichuan University

[ Objective] In order to study the susceptibility molecular mechanism of highly pathogenic porcine reproductive and respiratory syndrome virus （ HP- PRRSV） JXA1 isolate on Tibetan pig, Zangmei pig and Yorkshire pig. [ Method ] In the study, real-time quantitative RT-PCR method was established to compare and analyze the differential expression of five porcine reproductive and respiratory syndrome virus （PRRSV） receptor genes （HSPG2, SIGLEC1, CD163, VIM and NMMHC-H A） in lung tissues in Tibetan pig, Zangmei pig and Yorkshire pig before the challenge and at the 4th ,7th and 14th days after the challenge with JXAI isolate. [ Results ] HSPG2 expression in Tibetan pig lung tissues increased significantly at the 4th and 14th days after the challenge with JXAI （ P 〈 0.05 ）, while decreased significantly at the 7th day after the challenge （P 〈 0.05 ）, HSPG2 expression in Zangmei pig lung tissues increased significantly at the 14th day after the challenge （P〈0.05）. SIGLECl expression in Tibetan pig lung tissues increased significantly at the 4th and 14th days after the infection（P 〈 0.05 ）, while SIGLEC 1 expression in Yorkshire pig decreased significantly at the 4th, 7th and 14th days after the challenge （P 〈0. 05 ）. CD163 expression in lung tissues of Tibetan pig and Zangmei pig both increased significantly at the 14th day after the challenge （P 〈 0.05 ）, while CD163 expression in lung tissues of Yorkshire pig decreased significantly at the 7th and 14th days after the challenge （ P 〈 0. 05 ）. VIM expression in lung tissues of Tibetan pig increased significantly at the 7th day after the challenge （ P 〈 0. 05 ）, while which of Yorkshire pig at the 7th day after the challenge decreased significantly （ P 〈 0. 05 ）. NMMHC-II A expression in lung tissues of Zangmei pig increased significantly at the 4th day after the challenge （ P 〈 0. 05 ）, and which of Yorkshire pig increased significantly at the 4th and 14th days after the challenge （P 〈 0. 05 ）. [ Conclusion] SIGLEC1 and VIM

关键词： JXA1 isolate Tibetan pig Zangmei pig Yorkshire pig Porcine reproductive and respiratory syndrome virus receptor genes Differential expression

Mitigating greenhouse gas emissions from municipal wastewater treatment in China

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Environmental Science and Ecotechnology 2024年第4期20卷 333-340页

作者： Yindong Tong Xiawei Liao Yanying He Xiaomei Cui Marcus Wishart Feng Zhao Yulian Liao Yingxin Zhao xuebin lv Jiawen Xie Yiwen Liu Guanyi Chen Li'an Hou School of Ecology and Environment Tibet UniversityLhasa850012China School of Environmental Science&Engineering Tianjin UniversityTianjin300072China Bay Area International Business School Beijing Normal UniversityZhuhai519087China The World Bank Washington D.C.20433USA School of Mechanical Engineering Tianjin University of CommerceTianjin300134China

Municipal wastewater treatment plays an indispensable role in enhancing water quality by eliminating *** the process is vital,its environmental footprint,especially in terms of greenhouse gas(GHG)emissions,remains *** we offer a comprehensive assessment of GHG emissions from wastewater treatment plants(WWTPs)across *** analyses reveal an estimated 1.54(0.92-2.65)×10^(4)Gg release of GHGs(CO_(2)-eq)in 2020,with a dominant contribution from N_(2)O emissions and electricity *** can foresee a 60-65%reduction potential in GHG emissions with promising advancements in wastewater treatment,such as cutting-edge biological techniques,intelligent wastewater strategies,and a shift towards renewable energy sources.

关键词： Wastewater treatment Greenhouse gas emission Pollutant removal Advanced technology China

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超低浓度乙酸预处理玉米秸秆的研究

太阳能学报 2014年第6期35卷 931-936页

作者：王丽娟张书廷吕学斌天津大学环境科学与工程学院天津300072

以超低浓度乙酸作为催化剂对玉米秸秆进行预处理,考察不同温度和水解时间对秸秆水解效果的影响。结果表明:超低浓度乙酸对秸秆的水解具有较好的催化作用,当乙酸浓度为0.08%、温度为150℃、反应时间为60min时,水解液中葡萄糖、木糖、阿... 详细信息

以超低浓度乙酸作为催化剂对玉米秸秆进行预处理,考察不同温度和水解时间对秸秆水解效果的影响。结果表明:超低浓度乙酸对秸秆的水解具有较好的催化作用,当乙酸浓度为0.08%、温度为150℃、反应时间为60min时,水解液中葡萄糖、木糖、阿拉伯糖和乙酸的浓度分别达到13.38、17.07、5.33、11.86 g/L,而抑制物糠醛的浓度仅为0.2971 g/L。依据Saeman模型,计算得到木聚糖水解和木糖降解的动力学参数:活化能分别为110.57、118.97 k J/mol。

关键词：超低浓度乙酸玉米秸秆预处理活化能

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生物质水解液中糖酸分离方法的研究进展

太阳能学报 2007年第5期28卷 532-537页

作者：吕学斌张毅民张书廷天津大学化工学院绿色合成与转化教育部重点实验室天津300072 天津大学环境科学与工程学院天津300072

对生物质酸水解发酵制燃料乙醇过程中关键技术糖酸分离的方法进行了综述,对中和法、溶剂萃取法、离子排斥色谱法和双极性膜电渗析法的分离工艺过程以及优缺点进行了讨论。指出生物质浓酸水解制燃料乙醇工艺中使用离子排斥色谱法进行水... 详细信息

对生物质酸水解发酵制燃料乙醇过程中关键技术糖酸分离的方法进行了综述,对中和法、溶剂萃取法、离子排斥色谱法和双极性膜电渗析法的分离工艺过程以及优缺点进行了讨论。指出生物质浓酸水解制燃料乙醇工艺中使用离子排斥色谱法进行水解液中的糖酸分离以及酸回收是经济的;在生物质稀酸水解工艺中使用双极膜电渗析法分离水解液较使用离子排斥色谱法更为经济。同时,提出采用何种分离方法应根据所采用的水解条件和工艺要求进行选择才能达到经济实用的目的。

关键词：生物质酸水解糖酸分离离子排斥色谱法电渗析法

Expression changes of Caveolin family genes in longissimus dorsi muscle and back subcutaneous fat of two pig breeds

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Expression changes of Caveolin family genes in longissimus d...

利用ARIMA-SSA-LSTM组合模型的碳排放交易价格预测

中国畜牧兽医学会养猪学分会五届三次理事会暨生猪产业科技创新发展论坛

作者： Yiren Gu Yan Liang Xiaohui Chen Xuemei Yang Xuan Tao Zhiping He xuebin lv Sichuan Animal Science Academy

Caveolins have important roles in organization of detergent-insoluble lipid rafts,trafficking of cholesterol,and anchoring of signaling *** this study,expression patterns of Caveolin1(Cav-1), Caveolin 2(Cav-2) and Caveolin 3(Cav-3) genes were investigated in longissimus dorsi muscle and back subcutaneous fat of Berkshire and Yacha pigs at the age of 6 months using quantitative real-time PCR. The results showed that Cav-1 and -2 mRNA were abundantly expressed in back subcutaneous *** expression patterns of Cav -1 and -2 were similar in the two pig breeds and the expression levels in the back subcutaneous fat were significantly higher in Yacha pigs than that in Berkshire pigs(p < 0.01).The Cav-3 was expressed predominantly in the longissimus dorsi muscle,and the Berkshire pig had higher Cav-3 mRNA level compared with the Yacha pig(p < 0.01).These results indicate that the mRNAs of Cav family genes exhibit specific expression changes between Berkshire and Yacha pigs,and contribute to the elucidation of the relationship between meat quality and Cav family genes expression,also provides some data for further study on the mutual regulation mechanism of genes in Cav family.

关键词： longissimus dorsi muscle back subcutaneous fat Caveolin pig quantitative real-time PCR

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西安科技大学学报 2023年第5期43卷 1025-1034页

作者：炊婉冰吕学斌南京工业大学数理科学学院江苏南京211816

单一的预测方法在不同方面各有优劣,为了提高碳排放交易价格预测的精确度,从智能算法出发提出ARIMA-SSA-LSTM组合碳排放交易价格预测模型。该模型通过结合非线性规划局部搜索的优势和遗传算法全局搜索的优势使用非线性规划遗传算法分配... 详细信息

单一的预测方法在不同方面各有优劣,为了提高碳排放交易价格预测的精确度,从智能算法出发提出ARIMA-SSA-LSTM组合碳排放交易价格预测模型。该模型通过结合非线性规划局部搜索的优势和遗传算法全局搜索的优势使用非线性规划遗传算法分配差分整合移动平均自回归(ARIMA)模型和麻雀搜索算法优化后的长短时记忆(LSTM)模型(SSA-LSTM)的权重,通过加权得到最终的碳排放交易价格预测结果。运用ARIMA-SSA-LSTM组合模型,ARIMA模型,LSTM模型和SSA-LSTM模型分别对湖北省与广东省碳排放交易价格进行短期和长期预测。实证结果表明,相比单一的ARIMA模型、LSTM模型、SSA-LSTM模型,ARIMA-SSA-LSTM组合模型三个预测精度评价指标均为最小,碳排放交易价格预测精度最优。相比于传统ARIMA模型,机器学习LSTM模型具有更精确的预测结果,并且趋势预测更优。引入智能算法后,权重分配结果更加准确,LSTM模型的预测性能得到提升,印证了智能算法在碳排放交易价格预测领域的有效性。

关键词：应用统计碳排放交易价格预测加权组合非线性规划遗传算法麻雀算法 LSTM模型 ARIMA模型

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拉萨巴嘎雪湿地土壤重金属分布及生态风险评价

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环境化学 2021年第1期40卷 195-203页

作者：李伟布多孙晶单再毅吕学斌熊健西藏大学理学院拉萨850000

巴嘎雪湿地是西藏拉萨河流域的代表性湿地之一.文章为了解巴嘎雪湿地的重金属分布特征,在2018年10月采集了巴嘎雪湿地的表层土壤样品,对土壤中的Cu、Zn、Cr、Ni、Pb、Cd、As、Hg重金属的含量进行了检测.巴嘎雪湿地表层土壤的重金属污染... 详细信息

巴嘎雪湿地是西藏拉萨河流域的代表性湿地之一.文章为了解巴嘎雪湿地的重金属分布特征,在2018年10月采集了巴嘎雪湿地的表层土壤样品,对土壤中的Cu、Zn、Cr、Ni、Pb、Cd、As、Hg重金属的含量进行了检测.巴嘎雪湿地表层土壤的重金属污染评价采用的方法主要包括内梅罗综合污染指数法、地积累指数法和潜在生态风险指数法.结果表明,重金属Cu、Zn、Cr、Ni、As、Hg含量的平均值低于国家土壤环境质量标准(一级),但Pb、Cd的含量略偏高;Ni、As、Cr、Hg的平均含量低于拉萨市环境背景值,但Cu、Zn、Pb、Cd平均含量高于环境背景值.该研究区域的内梅罗综合污染指数平均值P_(N)=1.50,说明湿地整体处于轻度污染;按照国家土壤环境质量标准(二级)作为背景值,得到的P_(N)=0.64,属于安全水平.地积累指数平均值在-2.587到-0.744范围内,I_(geo)<0区域整体上无污染;潜在生态风险指数的平均值RI=72.779,属于低风险.各元素组合之间总体相关性不足,说明重金属累积方式具有多源性特点.巴嘎雪湿地整体生态环境优于国内大部分湿地,其表层土壤中Hg、Pb、Cd元素为强度变异,在部分位点中存在明显富集,受到人类活动影响,应加强关注.

关键词：巴嘎雪湿地重金属生态风险评价拉萨河流域

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粉煤灰高附加值利用研究进展

无机盐工业 2021年第4期53卷 25-31页

作者：冯文丽吕学斌熊健刘超于志昊张蕊西藏大学理学院西藏拉萨850000 天津城建大学环境与市政工程学院天津大学环境科学与工程学院

粉煤灰是火电厂燃煤过程中产生的固体残渣,其成分复杂,具有毒性,若处理不当会对环境造成危害。因此,粉煤灰的高附加值利用迫在眉睫。然而,粉煤灰的品质是制约其高附加值利用的主要因素。目前,中国粉煤灰品质参差不齐,缺少完善的品质评... 详细信息

粉煤灰是火电厂燃煤过程中产生的固体残渣,其成分复杂,具有毒性,若处理不当会对环境造成危害。因此,粉煤灰的高附加值利用迫在眉睫。然而,粉煤灰的品质是制约其高附加值利用的主要因素。目前,中国粉煤灰品质参差不齐,缺少完善的品质评价体系,由此造成粉煤灰利用领域的局限性。针对上述问题,首先分析了中国粉煤灰的资源化特性,总结了粉煤灰的品质评价方法,并重点阐述了适用于粉煤灰高附加值利用的品质评价方法;其次,详细介绍了粉煤灰高附加值利用技术(高附加值提取技术、高附加值材料制备技术);最后,对粉煤灰高附加值利用的发展趋势做出了展望。

关键词：粉煤灰品质评价高附加值利用