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Journal of Clinical and Translational Hepatology 2022年第6期10卷 1138-1147页

作者： Hao-Zhen Ren Sen-Zhe Xia xue-qian qin An-Yin Hu Jing-Lin Wang Department of Hepatobiliary Surgery Affiliated Drum Tower Hospital of Nanjing University Medical SchoolNanjingJi-angsuChina Department of Hepatobiliary Surgery Nanjing Drum Tower Hospital Clinical College of Jiangsu UniversityNanjingJiangsuChina Department of Hepatobiliary Surgery Nanjing Drum Tower Hospital Clinical College of Traditional Chinese and Western MedicineNanjing University of Chinese MedicineNanjingJiangsuChina

Background and Aims:Hepatic ischemic reperfusion in-jury(IRI)occurring during surgery seriously affects patient *** specific mechanism of IRI has not been fully *** study aim was to explore the changes of in-flammatory environment,and the relationship of the Th17/Treg cell ratio and FOXO1 expression in hepatic ***:Liver samples at different ischemic times were collected from patients and *** expression of inflammatory markers and FOXO1 in the liver was detected by western blotting and *** changes of liver lymphocytes were analyzed by flow *** AKT/Stat3/FOXO1 pathway was veri-fied by targeting AKT with *** role of FOXO1 in liver inflammation and changes in lymphocyte phenotype was confirmed by upregulating FOXO1 with ***-sults:Prolonged ischemic time aggravates liver injury in both humans and mouse models of hepatic ***-stress caused Th17/Treg imbalance and FOXO1 down-regulation by activat-ing the AKT/Stat3/FOXO1 signaling *** of FOXO1 reversed the Th17/Treg cytokine imbalance and altered the inflammation environment in the ***:Liver IRI induced Th17/Treg *** of FOXO1 re-versed the imbalance and alleviated liver inflammation.

关键词： Liver ischemia-reperfusion injury Inflammatory factors Th17 Treg AKT/Stat3/FOXO1 pathway

Detection of Shigella gyrA mutations and correlations between gyrA mutations and quinolones resistance

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Journal of Microbiology and Immunology 2005年第2期3卷 101-104页

作者： CHONG ZHEN WANG xue PING LI xue qin qian LI SHaG qian Department of Microbiology and Parasitology Shanghai Medical College Fudan University ShanghaiP. R. China

118 clinical strains of Shigella were serotyped, in which 116 strains were tested to be S. flexneri. The susceptibilities of the S .flexneri strains to quinolones were measured by the disk-diffusion method. It was found that most S .flexneri strains were susceptible to norfloxacin and ciprofloxacin, but resistant to nalidixic acid. To study the correlation between gyrA mutations and quinolones resistance, a fragment within the gyrA gene was amplified by PCR. The SSCP （Single-Strand Conformation Polymorphism） analysis was applied to detect mutations in PCR products of different strains. The mutations were then confirmed by DNA sequencing. Altogether, two types of mutation were revealed, in which one type was single mutation （ C42-T）, and the other was double mutations （ C42-T and A54- G）. By statistical analysis, C42-T （encoding Ser83-keu substitution） was shown to have correlation with nalidixic-acid resistance in the clinical strains of Shigella, while A54-G （encoding Asp87-Gly substitution） was shown to have correlations with both norfloxacin resistance and ciprofloxacin resistance.

关键词： Shigella Quinolones resistance PCR-SSCP gyrA mutations

Routinely detected indicators in plasma have a predictive effect on the identification of HIV-infected patients with non-tuberculous mycobacterial and tuberculous infections

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Infectious Diseases of Poverty 2017年第1期6卷 1172-1179页

作者： Ren-tian Cai Feng-xue Yu Zhen Tao xue-qin qian Jun Chen Hong-zhou Lu Department of Infectious Diseases Nanjing First HospitalNanjing Medical UniversityNanjingChina Department of Infectious Diseases Shanghai Public Health Clinical CenterFudan UniversityShanghaiChina Department of Nephrology the Second Affiliated Hospital of the Southeast UniversityNanjingChina Department of Mycobacteria Culture Shanghai Public Health Clinical CenterFudan UniversityShanghaiChina Huashan Hospital affiliated to Fudan University ShanghaiChina Medical College of Fudan University ShanghaiChina

Background:It is difficult to quickly distinguish non-tuberculous mycobacterial(NTM)infection from tuberculosis(TB)infection in human immunodeficiency virus(HIV)-infected patients because of many similarities between these diseases.A simple and effective way to determine the differences using routine blood tests is necessary in developing ***:A retrospective cohort study was conducted to recruit HIV-infected patients with either NTM infection or TB infection diagnosed for the first time according to mycobacterial culture and microscopic identification from May 2010 to March *** data included the analysis of blood cells,liver function,renal function,C-reactive protein(CRP),and erythrocyte sedimentation rate(ESR),and were compared between the HIV/TB and HIV/NTM ***:A total of 240 patients were *** number of HIV/TB and HIV/NTM patients was 113 and 127,*** were no significant differences in the CD4 T-cell count,age,sex,percentage of patients initiating antiretroviral therapy(ART)before the explicit diagnosis of TB or NTM *** infection was more likely to be restricted in the pulmonary while TB infection also involves extra-pulmonary *** the leukocyte count(5.60×109/L)and the proportion of neutrophils in the leukocyte count(76.70%)in the HIV/TB group were significantly higher than those in the HIV/NTM group(4.40×10^(9)/L[P=0.0014]and 69.30%[P<0.001].The analysis of liver function markers indicated that the concentration of albumin but not ALT and AST was significantly lower in the HIV/TB group than in the HIV/NTM group(P<0.001).The creatinine and urea levels were not significantly different between the two *** ESR(84.00 mm/h)and the concentration of CRP(59.60 mg/L)were significantly higher in the HIV/TB group than in the HIV/NTM group(52.00 mm/h and 19.60 mg/L,respectively)(P<0.001).To distinguish TB infection from NTM infection,the best cut-off value was 69.5 mm/h for ESR,with a positive pred

关键词： HIV Tuberculosis Non-tuberculous mycobacteria

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洛惠渠灌区典型样区地下水位与电导率关系研究

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水土保持通报 2011年第2期31卷 27-30页

作者：刘海波李占斌李鹏秦学谦张霞西安理工大学西北水资源与环境生态教育部重点实验室陕西西安710048 中国科学院水利部水土保持研究所黄土高原土壤侵蚀与旱地农业国家重点实验室陕西杨凌712100 黄河水利委员会三门峡库区水文水资源局河南三门峡472000 陕西省环境科学研究设计院陕西西安710061

洛惠渠灌区位于陕西省关中平原东部,由于不同区域的积盐过程不完全相同,针对洛惠渠灌区地下水的盐分变化情况,采用Morlet小波分析了灌区76#井的电导率和水位周期变化规律。结果表明,电导率变化和水位变化有很好的相关关系。小尺度上,水... 详细信息

洛惠渠灌区位于陕西省关中平原东部,由于不同区域的积盐过程不完全相同,针对洛惠渠灌区地下水的盐分变化情况,采用Morlet小波分析了灌区76#井的电导率和水位周期变化规律。结果表明,电导率变化和水位变化有很好的相关关系。小尺度上,水位变化周期和电导率的基本相同;大尺度上,水位约为电导率变化周期的2倍。两者变化存在明显的逆向位。该地区含有大量可溶性盐层,地下水电导率本底值较高。由于大气降水补给,电导率值随水位变化在一定的范围内波动。研究结果可为该灌区地表生态环境的改善提供依据。

关键词：洛惠渠地下水位电导率 Morlet小波

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浅谈涂装过程喷蜡防腐经验

现代涂料与涂装 2021年第11期24卷 62-64页

作者：秦学谦贾帅锋李琦奇瑞汽车河南有限公司河南开封475000

对涂装喷蜡工艺、生产过程管控与改善措施进行梳理、总结,使汽车车身防腐薄弱部位实现注蜡防护,有效提升车身耐腐蚀性能。

关键词：防腐喷蜡过程管控改善措施

十一方药酒对大鼠皮肤深Ⅱ度烫伤创面愈合的影响

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时珍国医国药 2007年第1期18卷 25-26页

作者：覃学谦蔡丹昭陈洪涛韦艾凌广西中医学院第一附属医院广西南宁530023 广西医科大学广西南宁530021 广西中医学院制药厂广西南宁530023

目的探讨十一方药酒对大鼠深Ⅱ度烫伤创面愈合的影响。方法大鼠深Ⅱ度烫伤模型分5组,创面分别外用高、中、低剂量的十一方药酒,以生理盐水为阴性对照,湿润烧伤膏为阳性对照,通过观察创面愈合时间,不同时间点创面愈合百分率和组织病理学... 详细信息

目的探讨十一方药酒对大鼠深Ⅱ度烫伤创面愈合的影响。方法大鼠深Ⅱ度烫伤模型分5组,创面分别外用高、中、低剂量的十一方药酒,以生理盐水为阴性对照,湿润烧伤膏为阳性对照,通过观察创面愈合时间,不同时间点创面愈合百分率和组织病理学变化,检测不同时间点创面组织羟脯氨酸水平,研究十一方药酒对大鼠深Ⅱ度烫伤创面愈合的影响。结果大鼠深Ⅱ度烫伤创面外用十一方药酒可以缩短愈合时间,提高创面愈合百分率,增加创面羟脯氨酸水平,作用与湿润烧伤膏相当。结论十一方药酒可增强胶原合成,促进上皮细胞增殖,加速烫伤创面愈合。

关键词：十一方药酒烫伤创面愈合羟脯氨酸

大孔吸附树脂分离纯化广山楂总黄酮的最佳工艺条件及参数探讨

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山东医药 2015年第8期55卷 7-10页

作者：陈洪涛刘源焕覃学谦蔡丹昭广西中医药大学制药厂南宁530023 广西中医药大学第一附属医院广西医科大学

目的探讨大孔吸附树脂分离纯化广山楂总黄酮的最佳工艺条件及参数。方法以静态和动态两个方面的饱和吸附量及洗脱率为指标,比较非极性树脂D101、弱极性树脂AB-8、中极性树脂HPD-450用于广山楂总黄酮分离纯化的优劣,并通过考察总黄酮回收... 详细信息

目的探讨大孔吸附树脂分离纯化广山楂总黄酮的最佳工艺条件及参数。方法以静态和动态两个方面的饱和吸附量及洗脱率为指标,比较非极性树脂D101、弱极性树脂AB-8、中极性树脂HPD-450用于广山楂总黄酮分离纯化的优劣,并通过考察总黄酮回收率,优化所选最佳树脂的吸附工艺参数。结果 D101、AB-8、HPD-450静态饱和吸附量分别为94.802、118.494、98.317 mg/g干树脂,静态洗脱率分别为83.434%、91.253%、86.831%,洗脱液总黄酮量分别为91.490、115.567、98.528 mg,总黄酮洗脱率分别为85.852%、93.217%、89.051%。以5倍树脂体积的70%乙醇洗脱,流速1.5 m L/min,所得总黄酮回收率为94.09%,纯度为73.77%。结论 AB-8型树脂综合性能最好,其最佳吸附工艺参数为以5倍树脂体积的70%乙醇洗脱,流速1.5 m L/min。

关键词：广山楂总黄酮大孔吸附树脂分离纯化

微波辅助提取紫马铃薯花色苷及其稳定性的研究

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食品科技 2011年第4期36卷 170-175页

作者：倪勤学高前欣霍艳荣陆国权浙江农林大学农业与食品科学学院临安311300

研究微波辅助提取紫马铃薯花色苷的工艺条件和稳定性。用pH示差法对紫马铃薯中的花色苷含量进行测定。正交实验结果表明:微波辅助提取的优化条件:提取时间40s,微波功率480W,料液比1:70,提取溶剂酸度0.11%盐酸水溶液,提取液花色苷含量达... 详细信息

研究微波辅助提取紫马铃薯花色苷的工艺条件和稳定性。用pH示差法对紫马铃薯中的花色苷含量进行测定。正交实验结果表明:微波辅助提取的优化条件:提取时间40s,微波功率480W,料液比1:70,提取溶剂酸度0.11%盐酸水溶液,提取液花色苷含量达到3.649mg/鲜紫马铃薯(g)。稳定性研究表明紫马铃薯花色苷具有较差的光稳定性和热稳定性,应避免光照和高温;氧化剂H2O2对其稳定性有较大的影响;在酸性条件下稳定性较好;金属离子和常用食品添加剂对紫马铃薯花色苷色素稳定性的影响较小。

关键词：紫马铃薯花色苷微波提取 pH示差法稳定性

龙血竭不同提取部位对高血糖模型小鼠血糖水平的影响

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时珍国医国药 2014年第9期25卷 2077-2078页

作者：陈洪涛刘源焕覃学谦朱智德蔡丹昭广西中医药大学制药厂广西南宁530023 广西中医药大学第一附属医院广西南宁530023 广西医科大学广西南宁530021

目的研究龙血竭不同提取部位对链脲佐菌素性高血糖模型小鼠血糖水平的影响。方法分别制备龙血竭石油醚、氯仿、乙酸乙酯提取部位,研究不同提取部位对链脲佐菌素性高血糖模型小鼠血糖、血脂水平及骨骼肌葡萄糖转运蛋白4(GLUT4)mRNA表达... 详细信息

目的研究龙血竭不同提取部位对链脲佐菌素性高血糖模型小鼠血糖水平的影响。方法分别制备龙血竭石油醚、氯仿、乙酸乙酯提取部位,研究不同提取部位对链脲佐菌素性高血糖模型小鼠血糖、血脂水平及骨骼肌葡萄糖转运蛋白4(GLUT4)mRNA表达的影响。结果龙血竭氯仿部位和乙酸乙酯部位能降低链脲佐菌素性高血糖小鼠空腹血糖水平,乙酸乙酯部位可上调模型小鼠GLUT4mRNA表达。结论龙血竭氯仿部位和乙酸乙酯部位具有降血糖功效。

关键词：龙血竭提取部位血糖

Revolutionizing breast cancer detection:Advances in fluorescent probe technology

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Nano Research 2025年第1期18卷 653-674页

作者： qian Gao Lin Tang Jun Zhan Yiyuan xue Feng Luo State Key Laboratory of Oral Diseases&National Center for Stomatology&National Clinical Research Center for Oral Diseases West China Hospital of StomatologySichuan UniversityChengdu 610041China Emergency Obstetrics and Gynecology West China Second University HospitalSichuan UniversityChengdu 610041China Department of Obstetrics and Gynecology West China Second University HospitalSichuan UniversityChengdu 610041China Department of Prosthodontics West China School of StomatologySichuan UniversityChengdu 610041China

With the increasing incidence of breast cancer globally,there is an urgent demand for enhanced diagnostic and therapeutic *** comprehensive review explores advancements in fluorescent probe technology for breast cancer imaging and early *** probes offer unparalleled sensitivity and specificity in detecting cancer biomarkers,enabling early diagnosis and delineation of tumor margins during *** categorize these probes into molecular,nano,ratiometric,the second near-infrared window(NIR-II),and multifunctional varieties,each with unique capabilities for targeting specific cancer *** review highlights recent innovations in probe design,emphasizing their applications in pathogenesis studies,drug development,precision medicine,and fluorescence-guided *** their promising clinical potential,challenges such as safety,biomarker targeting accuracy,system compatibility,and clinical translation *** these hurdles is crucial for the integration of fluorescent probes into standard cancer care,aiming to improve treatment outcomes and patient quality of life.

关键词： breast cancer fluorescent probes imaging early diagnosis biomarker