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Aptamer-Based Cell-Surface Profiling with Single-Cell Resolution Enables Precise Cancer Characterization

CCS Chemistry 2024年第1期6卷 196-207页

作者： Liujun Xu Yawei Feng Tong wang Shenhuan Li Kangli Xu Yue Sun Yi Luo Yishan Ye Yan Miao Yun Dong Zhenzhen Guo Qing Zhang Benshang Li He Huang xue-qiang wang Liping Qiu Weihong Tan Molecular Science and Biomedicine Laboratory State Key Laboratory of Chemo/Biosensing and ChemometricsCollege of Chemistry and Chemical EngineeringCollege of BiologyAptamer Engineering Center of Hunan ProvinceHunan UniversityChangshaHunan 410082 The Key Laboratory of Zhejiang Province for Aptamers and Theranostics Zhejiang Cancer HospitalHangzhou Institute of MedicineChinese Academy of SciencesHangzhouZhejiang 310022 Institute of Molecular Medicine Renji HospitalShanghai Jiao Tong University School of MedicineCollege of Chemistry and Chemical EngineeringShanghai Jiao Tong UniversityShanghai 200240 Bone Marrow Transplantation Center The First Affiliated HospitalSchool of MedicineZhejiang UniversityHangzhou 310003 Institute of Hematology Zhejiang UniversityHangzhou 310003 Department of Hematology and Oncology Shanghai Children’s Medical CenterShanghai Jiao Tong University School of MedicineShanghai Jiao Tong UniversityShanghai 200127

Molecular profiling of cell-surface proteins is a powerful strategy for precise cancer *** mass cytometry(MC)enables synchronous detection of over 40 cellular parameters,its full potential in disease classification is challenged by the limited types of recognition probes currently *** this work,we synthesize a panel of heavy isotopeconjugated aptamers to profile cancer-associated signatures on the surface of hematological malignancy(HM)*** on 15 molecular signatures,we performed cell-surface profiling that allowed the precise classification of 8 HM cell *** with machine-learning technology,this aptamer-based MC platform also achieved multiclass identification of HM subtypes in clinical sampleswith 100%accuracy in the training cohort and 80%accuracy in the test ***,we report an effective and practical strategy for precise cancer classification at the singlecell level,paving the way for its clinical use in the near future.

关键词： molecular profiling cancer diagnosis mass cytometry aptamers machine learning

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On the Asymptotic Behavior of the Storage Process Fed by a Markov Modulated Brownian Motion

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Acta Mathematicae Applicatae Sinica 2008年第1期24卷 75-84页

作者： Xiao-yu Xing xue-qiang wang School of Mathematical Sciences and LPMC Nankai University Tianjin 300071 China

Consider a storage model fed by a Markov modulated Brownian motion. We prove that the stationary distribution of the model exits and that the running maximum of the storage process over the interval [0, t] grows asymp... 详细信息

关键词： Markov modulated Brownian motion stationary distribution

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From Markov Jump Systems to Two Species Competitive Lotka-Volterra Equations with Diffusion

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Acta Mathematica Sinica,English Series 2009年第1期25卷 157-170页

作者： xue qiang wang Li Jun BO Yong Jin wang School of Mathematical Sciences and LPMC Nankai University Tianjin 300071 P. R. China Department of Mathematics Xidian University Xi'an 710071 P. R. China

In this paper, we start at a random evolution system on biological particles, which is described by a Markov jump system. Under a suitable scaling, we perform a proper approximation procedure. Then the so-called weak convergence of Markov processes and Martingales allow us to establish a （deterministic） two species competitive Lotka-Volterra equation.

关键词： Markov jump system competitive Lotka-Volterra equation weak convergence

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Defects Energetics and Electronic Properties of Li Doped ZnO： A Hybrid Hartree-Fock and Density Functional Study

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Chinese Journal of Chemical Physics 2012年第3期25卷 261-268,373页

作者： Xu Sun You-song Gu xue-qiang wang Yue Zhang Department of Material Physics and Chemist04 University of Science and Technology Beijing Beij'ing100083 China

The electronic properties and stability of Li-doped ZnO with various defects have been stud- ied by calculating the electronic structures and defect formation energies via first-principles calculations using hybrid Hartree-Fock and density functional methods. The results from formation energy calculations show that Li pair complexes have the lowest formation energy in most circumstances and they consume most of the Li content in Li doped ZnO, which make the p-type conductance hard to obtain. The formation of Li pair complexes is the main obstacle to realize p-type conductance in Li doped ZnO. However, the formation energy of Lizn decreases as environment changes from Zn-rich to O-rich and becomes more stable than that of Li-pair complexes at highly O-rich environment. Therefore, p-type conductance can be obtained by Li doped ZnO grown or post annealed in oxygen rich atmosphere.

关键词： Li doped ZnO Multi-defects Hybrid density functional

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Mechanical properties of Mn-doped ZnO nanowires studied by first-principles calculations

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International Journal of Minerals,Metallurgy and Materials 2012年第1期19卷 89-94页

作者： Zhan-jun Gao You-song Gu xue-qiang wang Yue Zhang School of Materials Science and Engineering University of Science and Technology BeijingBeijing 100083China

First-principles calculations were performed to investigate the mechanical properties of ZnO nanowires and to study the doping and size effects. A series of strains were applied to ZnO nanowires in the axial direction and the elastic moduli of ZnO nanowires were obtained from the energy versus strain curves. Pure and Mn-doped ZnO nanowires with three different diameters （1.14, 1.43, and 1.74 nm） were studied. It is found that the elastic moduli of the ZnO nanowires are 146.5, 146.6, and 143.9 GPa, respectively, which are slightly larger than that of the bulk （140.1 GPa）, and they increase as the diameter decreases. The elastic moduli of the Mn-doped ZnO nanowires are 137.6, 141.8, and 141.0 GPa, which are slightly lower than those of the undoped ones by 6.1%, 3.3%, and 2.0%, respectively. The mechanisms of doping and size effect were discussed in terms of chemical bonding and geometry considerations.

关键词： zinc oxide nanowires mechanical properties doping first-principles calculations manganese

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Hot Rolled Strip Re-reddening Temperature Changing Law during Ultra-fast Cooling

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Journal of Iron and Steel Research International 2015年第8期22卷 694-702页

作者： Lian-yun JIANG Chun-jiang ZHAO Jian-hui SHI Guo YUAN xue-qiang wang Qing-xue HUANG State Key Laboratory of Rolling and Automation Northeastern University School of Mechanical Engineering Taiyuan University of Science and Technology Shougang Qian'an Iron and Steel Co. Ltd.

Temperature deviation between surface and the center of hot rolled strip is formed during ultra-fast cooling （UFC）. Surface temperature would rise when temperature deviation goes up to an extent, and strip re-reddening phenomenon will appear. Strip re-reddening affects the stability of strip microstructure, property and temperature control precision. Thus, it is necessary to conduct research on re-reddening temperature changing law to improve strip property and temperature control precision. Strip temperature trends for various strip thicknesses and ultra-fast cooling rates were obtained by numerical calculation method. Re-reddening temperature, temperature deviation between surface and center, and boundary layer position changing law were obtained. By comparison, some conclusions were obtained： UFC re-reddening temperature and laminar cooling （LC） re-reddening temperature were linear to ultra-fast cooling rate respectively. Ultra-fast cooling rate affected UFC re-reddening temperature greatly, but it had little effect on LC re-reddening temperature. Equations which were used to calculate UFC re-reddening temperature, LC re-reddening temperature and maximum temperature deviation were obtained. The position of boundary layer stayed in 1/4 strip thickness.

关键词： ultra-fast cooling laminar cooling cooling rate re-reddening temperature

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Viscosity and structure of CaO-BaO-SiO_(2)-Al_(2)O_(3)-based mold slags for continuous casting of high titanium steel with different Tio2 absorption

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Journal of Iron and Steel Research International 2024年第8期31卷 1936-1946页

作者： Ya-tao Cui xue-you wang Xu-bin Zhang qiang-qiang wang Sheng-ping He College of Materials Science and Engineering Chongqing UniversityChongqing 400044China

The effect of TiO_(2) absorption into two different CaO-BaO-SiO_(2)-Al_(2)O_(3)-based mold slags from the steel plant on the viscosity,melting performance and microstructure of slags was investigated through the measurement of the viscosity-temperature relationship,melting temperature and Raman *** parameter of the number of non-bridging oxygen per tetrahedrally-coordinated atom(NBO/T)was also calculated to explain the microstructure variation of molten slags with different TiO_(2) *** variation of the actual slag consumption and the depth of the liquid slag in mold was explained through the comparison of the viscosity and the melting temperature of two different *** viscosity of mold slags(basicity=0.6)decreased from 1.1 to 0.68 Pa s with the increase in the Ti0_(2) absorption from 0 to 10%,while that of slags(basicity=0.7)decreased from 0.76 to 0.56 Pa s with the Ti0_(2) absorption from 0 to 6%.The activation energy of both two groups of slags had the tendency to decrease with the increasing TiO_(2) *** network structure of both two groups of slags measured by the Raman spectra showed that the fraction of complex structure units(Q^(1),Q^(2),Q^(3) and A1-O-Al)decreased and simple structure units(Al-O-and Q^(0))increased with the increase in TiO_(2) ***/T also increased with the increase in the TiO2 absorption,indicating that the absorption of TiO_(2) into slags resulted in the destruction of silicate/aluminate ***,the absorption of TiO_(2) into the current CaO-BaO-SiO_(2)-Al_(2)O_(3) mold slags decreased the degree of polymerization of these slags and then led to the decrease of viscosity.

关键词： Mold slag Viscosity Melting temperature Slag consumption Liquid slag depth Structure

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A versatile strategy for convenient circular bivalent functional nucleic acids construction

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National Science Review 2023年第2期10卷 146-156页

作者： Xiao-Jing Zhang Zhuo Zhao Xia wang Min-Hui Su Lili Ai Yingying Li Quan Yuan xue-qiang wang Weihong Tan Molecular Science and Biomedicine Laboratory (MBL) State Key Laboratory of Chemo/Biosensing and ChemometricsCollege of Chemistry and Chemical Engineering College of Biology Aptamer Engineering Center of Hunan ProvinceHunan University Institute of Molecular Medicine (IMM) Renji HospitalShanghai Jiao Tong University School of Medicine and College of Chemistry and Chemical EngineeringShanghai Jiao Tong University The Cancer Hospital of the University of Chinese Academy of Sciences (Zhejiang Cancer Hospital) Hangzhou Institute of Medicine (HIM)Chinese Academy of Sciences

Functional nucleic acids(FNAs),such as aptamers,nucleic acid enzymes and riboswitches play essential roles in various fields of life *** of ingenious chemical moieties toward FNAs can enhance their biomedical properties and/or confer them with exogenic biological functions that,in turn,can considerably expand their biomedical applications,or even improve their clinical ***,we report the first example of a general chemical tailoring strategy that enables the divergent ligation of D NA *** applying this technology,different types of aptamers and single-stranded nucleic acids of various lengths could be efficiently tailored to deliver the designed circular bivalent aptamers(CBApts) and cyclized DNA se quences with high *** is worth noting that CBApts exhibited significantly enhanced nuclease resistance,as well as considerably improved binding,targeting and tumor tissue enrichment abilities,which may pave the way for different investigations for biomedical purposes.

关键词： circular bivalent aptamers click chemistry chemical ligation

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Regulating the properties of XQ-2d for targeted delivery of therapeutic agents to pancreatic cancers

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National Science Review 2023年第8期10卷 166-172页

作者： Qiuxia Yang Yongbo Peng Zhengyu Deng Dailiang Zhang Cheng-Yu Long Guo-Rong Zhang Juan Li xue-qiang wang Weihong Tan Zhejiang Cancer Hospital Hangzhou Institute of Medicine(HIM) Chinese Academy of Sciences Molecular Science and Biomedicine Laboratory(MBL) State Key Laboratory of Chemo/Biosensing and ChemometricsCollege of Chemistry and Chemical Engineering College of Biology Aptamer Engineering Center of Hunan ProvinceHunan University Institute of Molecular Medicine(IMM) Renji HospitalSchool of MedicineCollege of Chemistry and Chemical Engineering Shanghai Jiao Tong University

Enhanced recognition ability, cell uptake capacity, and biostability are characteristics attributed to aptamer-based targeted anticancer agents, and are possibly associated with increased accumulation at the tumor site, improved therapeutic efficacy and reduced negative side effects. Herein, a phosphorothioate backbone modification strategy was applied to regulate the biomedical properties of pancreatic cancer cell–targeting aptamer for efficient in vivo drug delivery. Specifically, the CD71-targeting aptamer XQ-2d was modified into a fully thio-substituted aptamer S-XQ-2d, improving the plasma stability of S-XQ-2d and mitomycin C(MMC)-functionalized S-XQ-2d(MFSX), thus considerably prolonging their half-life in mice. Moreover, the binding and uptake capacities of S-XQ-2d were significantly enhanced. MFSX showed the same level of cytotoxicity as that of MMC against targeted cancer cells, but lower toxicity to non-targeted cells, highlighting its specificity and biosafety. Brief mechanistic studies demonstrated that XQ-2d and S-XQ-2d had different interaction modes and internalization pathways with the targeted cells.

关键词： phosphorothiolation MMC-functionalized aptamer targeted pancreatic cancer therapy

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A new paradigm for artesunate anticancer function:considerably enhancing the cytotoxicity via conjugating artesunate with aptamer

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Signal Transduction and Targeted Therapy 2021年第10期6卷 2964-2966页

作者： Yingying Li Yongbo Peng Yan Tan Wenjing Xuan Ting Fu xue-qiang wang Weihong Tan Molecular Science and Biomedicine Laboratory(MBL) State Key Laboratory of Chemo/Bio-Sensing and ChemometricsCollege of Chemistry and Chemical EngineeringCollege of BiologyAptamer Engineering Center of Hunan ProvinceHunan UniversityChangshaPR China Institute of Molecular Medicine(IMM) Renji HospitalShanghai Jiao Tong University School of Medicineand College of Chemistry and Chemical EngineeringShanghai Jiao Tong UniversityShanghaiPR China The Cancer Hospital of the University of Chinese Academy of Sciences(Zhejiang Cancer Hospital) Institute of Basic Medicine and Cancer(IBMC)Chinese Academy of SciencesHangzhouZhejiangChina

Dear Editor,Artemisinin and its derivatives(AIDs)have recently been widely applied in cancer therapy as promising therapeutic agents owing to its hypotoxicity and special bioactivation *** numerous strategies have been established to improve the potency of AIDs,they are still relatively inefficacious against cancers,especially as a monotherapy.^(1)It is thus essential to develop novel approaches to considerably enhance their anticancer *** hypothesized that aptamers with specific recognition,high-affinity binding,and receptor-mediated internalization would promote the accumulation of AIDs inside the target cells and lead to death of the target cancer cells.^(2)

关键词： artesunate considerably cytotoxicity

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