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Two New Dammarane Glycosides from the Acid Hydrolysis Product of Panax Notoginseng

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Chinese Chemical Letters 2001年第3期12卷 239-242页

作者： Rong Wei TENG Hai Zhou LI xue mei zhang Xi Kui LIU De Zu WANG Chong Ren YANG Kunming Institute of Botany Chinese Academy of SciencesKunming 650204

Two new dammarane glycosides named notoginsenoside T-1 and T-2 were isolated from the mild acid hydrolysis products of the root saponins of Panax notoginseng. On the basis of spectroscopic evidences, their structures were elucidated to be 6-O-beta -D-glucopyranosyl-24(25)-epoxy-3 beta ,6 alpha ,12 beta ,23 xi -tetrahydroxydammar-20(22)(E)-ene 1 and 6-O-beta -D-glucopyranosyl-24(25)-epoxy-23 xi -methoxyl-3 beta ,6 alpha ,12 beta -trihydroxydamm-ar-20(22)(E)-ene 2, respectively.

关键词： Panax notoginseng dammarane glycosides notoginsenoside T1 T2.

Complete Genomic Sequence Analysis of a New SV40 Isolate

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中国病毒学 2007年第1期22卷 41-45页

作者： xue-mei zhang Yan-chun CHE Jing-jing WANG Long-ding LIU Ming-xue XIE Qi-han LI Institute of Medical Biology Chinese Academy of Medical Sciences and Peking Union Medical College Kunming Yunnan Province 650118 China

从恒河猴孤立的一个新 SV40 种类(SV-IMB ) 的染色体完全被定序并且与其它相比孤立。结果证明整个染色体包含 5246bp，并且 SV-IMB 的 aver 年龄身份作为与另外的 SV40 相比是 98.1% 孤立。它的规章的区域由完全的 enhancer 和有缺点的 ... 详细信息

从恒河猴孤立的一个新 SV40 种类(SV-IMB ) 的染色体完全被定序并且与其它相比孤立。结果证明整个染色体包含 5246bp，并且 SV-IMB 的 aver 年龄身份作为与另外的 SV40 相比是 98.1% 孤立。它的规章的区域由完全的 enhancer 和有缺点的 e enhancer 组成。氨基酸变化在大 T 抗原(标签) 和 VP1 区域发生了到某程度。调查结果证明 SV-IMB 是新 SV40 孤立。关键词 SV40 - 标签 - 规章的区域 CLC 数字 S852.65 基础条款：国家自然科学成立(30570081 )

关键词： SV40 T-Ag Regulatory region

Diarylheptanoid-flavanone Hybrids as Multiple-target Antidiabetic Agents from Alpinia katsumadai

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Chinese Journal of Chemistry 2021年第11期39卷 3051-3063页

作者： Xiao-Feng He Ji-Jun Chen Tian-Ze Li Jing Hu Xiao-Yan Huang xue-mei zhang Yuan-Qiang Guo Chang-An Geng State Key Laboratory of Phytochemistry and Plant Resources in West China Kunming Institute of BotanyChinese Academy of SciencesYunnan Key Laboratory of Natural Medicinal ChemistryKunmingYunnan650201 China College of Pharmacy and Tianjin Key Laboratory of Molecular Drug Research Nankai UniversityTianjin300071 China University of Chinese Academy of Sciences Beijing100049 China

The EtOAc fraction of Alpinia katsumadai seeds showed significant inhibition on glycogen phosphorylase a(GPa)with inhibitory ratios of 97.9%and 64.6%at concentrations of 200 and 100μg/mL,***-guided isolation afforded 15 new diarylheptanoid-flavanone hybrids,katsumadainols B_(1)-B_(15)(1-15),together with eight known ones(16-23).Compounds 4-10 and 12-21 exhibited activity against GPa with IC_(50) values of 10.1-95.4μmol/L;compounds 4,5,16,and 17 displayed inhibitory effects onα-glucosidase with IC_(50) values of 7.1,12.4,7.2,and 8.3μmol/L,obviously higher than acarbose(IC_(50),209.1μmol/L);compounds 4-6,14,16-20,22,and 23 were PTP1B/TCPTP selective inhibitors with IC_(50) values of 40.7-95.8μmol/L;compounds 4,5,16,and 17 showed DPP4 inhibitory effects with inhibitory ratios of 50.0%-54.2%(200μmol/L).Diarylheptanoid-flavanone hybrids(4,5,16,and 17)with a p-hydroxybenzyl at C-6 position represent a promising class of multiple-target antidiabetic agents inhibiting GPa,α-glucosidase,PTP1B,and DPP4.

关键词： Alpinia katsumadai Katsumadainols Bx-B_(15) Natural products Structure elucidation Biological activity

Therapeutic effects of dental pulp stem cells on vascular dementia in rat models

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Neural Regeneration Research 2021年第8期16卷 1645-1651页

作者： xue-mei zhang Yang Sun Ying-Lian Zhou Zhuo-Min Jiao Dan Yang Yuan-Jiao Ouyang mei-Yu Yu Jin-Yue Li Wei Li Duo Wang Hui Yue Jin Fu Department of Neurology The 2nd Affiliated Hospital of Harbin Medical UniversityHarbinHeilongjiang ProvinceChina

Dental pulp stem cells are a type of adult stem cells with strong proliferative ability and multi-differentiation potential. There are no studies on treatment of vascular dementia with dental pulp stem cells. In the present study, rat models of vascular dementia were established by two-vessel occlusion, and 30 days later, rats were injected with 2 × 10^(7) dental pulp stem cells via the tail vein. At 70 days after vascular dementia induction, dental pulp stem cells had migrated to the brain tissue of rat vascular dementia models and differentiated into neuronlike cells. At the same time, doublecortin, neurofilament 200, and Neu N m RNA and protein expression levels in the brain tissue were increased, and glial fibrillary acidic protein m RNA and protein expression levels were decreased. Behavioral testing also revealed that dental pulp stem cell transplantation improved the cognitive function of rat vascular dementia models. These findings suggest that dental pulp stem cell transplantation is effective in treating vascular dementia possibly through a paracrine mechanism. The study was approved by the Animal Ethics Committee of Harbin Medical University(approval No. KY2017-132) in 2017.

关键词： animal model dental pulp stem cells paracrine repair stem cells transplantation vascular dementia

Minor secoiridoid aglycones from the low-polarity part of the traditional Chinese herb: Swertia mileensis

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Natural Products and Bioprospecting 2013年第5期3卷 243-249页

作者： Chang-An GENG xue-mei zhang Yun-Bao MA Xiao-Yan HUANG Ji-Jun CHEN State Key Laboratory of Phytochemistry and Plant Resources in West China Kunming Institute of BotanyChinese Academy of SciencesKunming 650201China

Eleven new secoiridoid aglycones involving unusual C9-skeleton:swerimilegenins A-F(1-6);bis-C9-skeleton:swerimilegenin G(7);and C_(10)-skeleton:swerimilegenins H−K(8−11),as well as six known ones,were isolated from the low-polarity part of the traditional Chinese herb medicine Swertia *** structures were determined by extensive spectroscopic data and X-ray ***,swerimilegenin A(1)belonged to 10-nor-secoiridoid,and swerimilegenins B-F(2-6)were ***(12)and gentiogenal(15)showed moderate anti-HBV activity on HepG 2.2.15 cell line in vitro.

关键词： Swertia mileensis swerimilegenins secoiridoid aglycones anti-HBV activity

An AIEgen nano-assembly for simultaneous detection of ATP and H_(2)S

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Chinese Chemical Letters 2024年第6期35卷 376-379页

作者： Jia-mei Qin xue Li Wei Lang Fu-Hao zhang Qian-Yong Cao School of Chemistry and Chemical Engineering Nanchang UniversityNanchang 330031China

A new aggregation-induced emission(AIE)-based fluorescence sensor,TPEPy-SS-C14,for simultaneous recognition of adenosine triphosphate(ATP)and hydrogen sulfide(H_(2)S)has been reported via the aggregation-disaggregation *** probe self-assembles nano-structure aggregations in aqueous *** shows fluorescence turn-on response toward ATP for the complexation-enhanced aggregation,but leads to fluorescence quenching of H_(2)S for cleavage the aggregations.

关键词： AIE-based ATP detection H_(2)S detection Fluorescent sensor Cell imaging

Artemiprincepsolides A-F,Novel Germacrane-guaiane and Eudesmane-guaiane Sesquiterpenoid Dimers from Artemisia princeps and Their Antihepatoma Activity

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Chinese Journal of Chemistry 2023年第20期41卷 2648-2656页

作者： Li-Hua Su Wen-Jing Ma Yun-Bao Ma Tian-Ze Li Chang-An Geng Wei Dong Xiao-Feng He xue-mei zhang Ji-Jun Chen State Key Laboratory of Phytochemistry and Plant Resources in West China Kunming Institute of BotanyChinese Academy of SciencesKunmingYunnan650201 China University of Chinese Academy of Sciences Beijing100049 China

Artemiprincepsolides A-F(1-6)were isolated from Artemisia princeps guided by bioactivity and elucidated by comprehensive spectral data and ECD *** 1-3 represented the first connecting model of germacrane-guaiane hetero-dimeric adducts,and compounds 4-6 were eudesmane-guaiane hetero-coupled sesquiterpenoid dimers,meanwhile,all these were presumably formed by Diels-Alder *** 1-6 were evaluated for their hepatomatic cytotoxicity on three hepatoma cell lines,and demonstrated cytotoxicity with IC_(50)values in the range of 5.0-67.3μmol/***,compound 1 manifested significant cytotoxicity against HepG2,Huh7,and SK-Hep-1 cells with IC_(50)values of 9.9,9.2,and 5.0μmol/L,which were almost equivalent to the positive control,*** cytometry data and Western blot assays revealed the most active compound 1 dose-dependently inhibited cell migration and invasion,and significantly induced HepG2 cells arrest in G2/M phase by downregulating proteins pcdc2 and upregulating the level of protein CyclinB1;and induced apoptosis by downregulating of Bcl-2 expression and upregulating Bax level.

关键词： Artemisia princeps Hetero-coupled sesquiterpenoid dimers Structure elucidation Stereochemistry Artemiprincepsolides Biological activity Antihepatoma activity Apoptosis

Study on the Effect of Ginaton on Reducing Cerebral Vasospasm and Early Brain Injury after Hemorrhagic Stroke by Inhibiting Inflammatory Response

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World Journal of Traditional Chinese Medicine 2024年第1期10卷 33-39页

作者： xue-Bo Pang Xiao-Lin zhang mei-Rong Wang Ying Yuan Xiao zhang Department of Neurology Xingtai Third HospitalXingtaiChina

Objective:The objective of this study was to investigate the effects and possible mechanisms of action of ginseng on cerebral vasospasm and early brain injury(EBI)following hemorrhagic *** and Methods:Sprague-Dawley(SD)rats(n=48)were randomly divided into sham operation(sham group),subarachnoid hemorrhage(SAH)model(SAH group),normal saline(NS group),and Ginaton(Extract of Ginkgo Biloba Leaves Drops)intervention(gin group)***-1 m RNA and tumor necrosis factor levels were detected using reverse transcription-polymerase chain *** relative expression of m RNA was detected by Western ***:(1)Compared with the sham group,the SAH,NS,and gin groups had different degrees of neurological *** with the SAH and NS groups,the neurological deficits in the gin group were significantly improved(P<0.05).(2)Compared with the sham group,the relative expression levels of MCP-1 m RNA in the SAH,NS,and gin groups were 5.1±0.9,3.4±0.6,and 2.5±0.4,respectively;the relative expression levels of m RNA were 13.3±2.4,11.2±1.8,and 3.8±0.6,respectively.(3)The apoptosis rates of brain tissue in the sham,SAH,NS,and gin groups were 4.8±0.7,54.2±10.3,50.1±7.4,and 28.4±4.5,respectively.(4)Western blot showed that the relative expression levels of toll-like receptor-4(TLR-4)protein in the sham,SAH,NS,and gin groups were 0.29±0.03,0.87±0.15,0.65±0.13,and 0.41±0.17,respectively;the relative expression levels of B protein were 0.21±0.04,0.96±0.14,0.73±0.18,and 0.30±0.05,*** treatment could inhibit TLR-4 and nuclear factor-κB(NF-κB)protein ***:Dona tablets may inhibit activation of the NF-κB signaling pathway,and SAH-induced inflammatory response,so as to reduce cerebral vasospasm and EBI.

关键词： Cerebral hemorrhage early brain injury ginaton neurological deficit nuclear factor-κB

Herpes Simplex Virus 1 Infection Alters the mRNA Translation Processing in L-02 Cells

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Virologica Sinica 2008年第1期23卷 43-50页

作者： Min HONG Yan-chun CHE Gui-zhen TANG Wei CUN xue-mei zhang Long-ding LIU Qi-han LI Institute of Medical Biology Chinese Academy of Medical Sciences Peking Union Medical College Kunming 650118 China

HSV-1 infection-mediated regulation of mRNA translation in host cells is a systematic and complicated process. Investigation of the details of this mechanism will facilitate understanding of biological variations in the viral replication process and host cells. In this study, a comparative proteomics technology platform was applied by two-dimension electrophoresis of HSV-1 infected normal human L-02 cell and control cell lysates. The observed protein spots were analyzed qualitatively and quantitatively by the PDQuest software package. A number of the different observed protein spots closely associated with cellular protein synthesis were identified by matrix-assisted laser-desorption ionization-time of flight-mass spectrometry (MALDI-TOF-MS). The expression levels of the RPLP1 protein, which is required for mRNA translation, and KHSRP protein, which is involved in rapid decay of mRNA, were up-regulated, whereas the expression level of RNP H2, which is involved in positive regulation on the mRNA splicing process, was down-regulated. All of these results suggest that HSV-1 infection can influence cellular protein synthesis via modulation of cellular regulatory proteins involved in RNA splicing, translation and decay, resulting in optimisation of viral protein synthesis when cellular protein synthesis is shut off. Although there is need for further investigations regarding the detailed mechanisms of cellular protein control, our studies provide new insight into the targeting of varied virus signaling pathways involved in host cellular protein synthesis.

关键词： HSV-1 L-02 cell Comparative proteomics