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Isolation and characterization of a novel strain(YH01) of Micropterus salmoides rhabdovirus and expression of its glycoprotein by the baculovirus expression system

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2019年第9期20卷 728-739页

作者： Sun-jian LYU xue-mei yuan Hai-qi ZHANG Wei-da SHI Xiao-ying HANG Li LIU Ying-lei WU Agriculture Ministry Key Laboratory of Healthy Freshwater Aquaculture Key Laboratory of FishHealth and Nutrition of Zhejiang ProvinceZhejiang Institute of Freshwater Fisheries

As one of the most important aquatic fish,Micropterus salmoides suffers lethal and epidemic disease caused by rhabdovirus at the juvenile *** this study,a new strain of *** rhabdovirus(MSRV)was isolated from Yuhang,Zhejiang Province,China,and named *** virus infected the grass carp ovary(GCO)cell line and displayed virion particles with atypical bullet shape,300–500 nm in length and 100–200 nm in diameter under transmission electron *** complete genome sequence of this isolate was determined to include 11 526 nucleotides and to encode five classical structural *** construction of the phylogenetic tree indicated that this new isolate is clustered into the Vesiculovirus genus and most closely related to the Siniperca chuatsi *** explore the potential for a vaccine against MSRV,a glycoprotein(1–458 amino acid residues)of MSRV-YH01 was successfully amplified and cloned into the plasmid *** high-purity recombinant bacmid-glycoprotein was obtained from DH10Bac through screening and *** on polymerase chain reaction(PCR),western blot,and immunofluorescence assay,recombinant virus,including the MSRV-YH01 glycoprotein gene,was produced by transfection of SF9 cells using the pFastBac1-gE2,and then repeatedly amplified to express the glycoprotein *** anticipate that this recombinant bacmid system could be used to challenge the silkworm and develop a corresponding oral vaccine for fish.

关键词： Micropterus salmoides Rhabdovirus Glycoprotein Baculovirus system

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Analysis of the influencing factors and clinical related characteristics of pulmonary tuberculosis in patients with type 2 diabetes mellitus

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World Journal of Diabetes 2024年第2期15卷 196-208页

作者： Han Shi yuan yuan xue Li Yan-Fang Li Ling Fan xue-mei Yang Department of Infectious Diseases The First Affiliated Hospital of Chengdu Medical CollegeChengdu 610500Sichuan ProvinceChina

BACKGROUND In China,the prevalence of type 2 diabetes mellitus(T2DM)among diabetic patients is estimated to be between 90%-95%.Additionally,China is among the 22 countries burdened by a high number of tuberculosis cases,with approximately 4.5 million individuals affected by active ***,T2DM poses a significant risk factor for the development of tuberculosis,as evidenced by the increased incidence of T2DM coexisting with pulmonary tuberculosis(T2DMPTB),which has risen from 19.3%to 24.1%.It is evident that these two diseases are intricately interconnected and mutually reinforcing in *** To elucidate the clinical features of individuals diagnosed with both T2DM and tuberculosis(T2DM-PTB),as well as to investigate the potential risk factors associated with active tuberculosis in patients with *** T2DM-PTB patients who visited our hospital between January 2020 and January 2023 were selected as the observation group,Simple DM patients presenting to our hospital in the same period were the control group,Controls and case groups were matched 1:2 according to the principle of the same sex,age difference(±3)years and disease duration difference(±5)years,patients were investigated for general demographic characteristics,diabetes-related characteristics,body immune status,lifestyle and behavioral habits,univariate and multivariate analysis of the data using conditional logistic regression,calculate the odds ratio(OR)values and 95%CI of OR *** A total of 315 study subjects were included in this study,including 105 subjects in the observation group and 210 subjects in the control *** of the results of both anthropometric and biochemical measures showed that the constitution index,systolic blood pressure,diastolic blood pressure and lymphocyte count were significantly lower in the case group,while fasting blood glucose and high-density lipoprotein cholesterol levels were significantly higher than those in the control ***

关键词： Type 2 diabetes Pulmonary tuberculosis Blood sugar Infection Risk factors

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STUDY OF COMPLEXATION OF BARBITURATES WITH β-CYCLODEXTRIN

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Chinese Chemical Letters 1993年第12期4卷 1085-1088页

作者： xue mei WANG Hong yuan CHEN Department of Chemistry,Nanjing University,210008 Nanjing

The nature of the quenching of barbiturate fluorescence by forming inclusion complexcs with β-cyclodextrin is investigated principally using fluorescence/absorption and Fourier transform infrared(FTIR)*** relatively weak complexes formed in aqueous solutions indicated through the IR spectroscopies are confirmed by the apparent formation constants acquired by using fluorescence *** specific information concerning the mechanisms of interaction has been ex- plored in detail by means of the analysis of the electrochemical and spectroscopic behaviour.

关键词： CYCLODEXTRIN STUDY OF COMPLEXATION OF BARBITURATES WITH TIR

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CHARACTERIZATION OF THE β-CYCLODEXTRIN/QUININE COMPLEX

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Chinese Chemical Letters 1994年第5期5卷 399-402页

作者： xue mei WANG and Hong yuan CHEN (Department of Chemistry, Nanjing University. Nanjing 210008)

Fourier transform infrared (FTIR) studies of the complexation of quinine hydtoohloride with β-cyclodextrin (β-CD) in aqueous solution have been carried out, it can afford direct evidence relative to the molecular structure of the complex as well as fresh insight into the molecular interactions of the cyclodextrin system.

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Study on the Effect of Ginaton on Reducing Cerebral Vasospasm and Early Brain Injury after Hemorrhagic Stroke by Inhibiting Inflammatory Response

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World Journal of Traditional Chinese Medicine 2024年第1期10卷 33-39页

作者： xue-Bo Pang Xiao-Lin Zhang mei-Rong Wang Ying yuan Xiao Zhang Department of Neurology Xingtai Third HospitalXingtaiChina

Objective:The objective of this study was to investigate the effects and possible mechanisms of action of ginseng on cerebral vasospasm and early brain injury(EBI)following hemorrhagic *** and Methods:Sprague-Dawley(SD)rats(n=48)were randomly divided into sham operation(sham group),subarachnoid hemorrhage(SAH)model(SAH group),normal saline(NS group),and Ginaton(Extract of Ginkgo Biloba Leaves Drops)intervention(gin group)***-1 m RNA and tumor necrosis factor levels were detected using reverse transcription-polymerase chain *** relative expression of m RNA was detected by Western ***:(1)Compared with the sham group,the SAH,NS,and gin groups had different degrees of neurological *** with the SAH and NS groups,the neurological deficits in the gin group were significantly improved(P<0.05).(2)Compared with the sham group,the relative expression levels of MCP-1 m RNA in the SAH,NS,and gin groups were 5.1±0.9,3.4±0.6,and 2.5±0.4,respectively;the relative expression levels of m RNA were 13.3±2.4,11.2±1.8,and 3.8±0.6,respectively.(3)The apoptosis rates of brain tissue in the sham,SAH,NS,and gin groups were 4.8±0.7,54.2±10.3,50.1±7.4,and 28.4±4.5,respectively.(4)Western blot showed that the relative expression levels of toll-like receptor-4(TLR-4)protein in the sham,SAH,NS,and gin groups were 0.29±0.03,0.87±0.15,0.65±0.13,and 0.41±0.17,respectively;the relative expression levels of B protein were 0.21±0.04,0.96±0.14,0.73±0.18,and 0.30±0.05,*** treatment could inhibit TLR-4 and nuclear factor-κB(NF-κB)protein ***:Dona tablets may inhibit activation of the NF-κB signaling pathway,and SAH-induced inflammatory response,so as to reduce cerebral vasospasm and EBI.

关键词： Cerebral hemorrhage early brain injury ginaton neurological deficit nuclear factor-κB

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PHYLETIC RELATIONSHIP OF PROTEIN STRUCTURES BASED ON SPATIAL PREFERENCE OF RESIDUES

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Chinese Chemical Letters 1991年第11期2卷 867-870页

作者： Chun Xu QU Lu Hun LAI Xiao Jie XU xue mei yuan You Qi TANG Institute of Physical Chemistry Peking UniversityBeijing 100871

A distance measure that infers to indicate the evolutionary relationship of protein structures has been developed based on spatial preference factors of residues. The spatial preference factor is a reflection of the environment of residues in tertiary structure. Compared with the phyletic relationships derived from sequence homologies and three-dimensional structures, we find that the two lines of evolution are similar in general. This approach is applied to a group of glins here.

关键词： PHYLETIC RELATIONSHIP OF PROTEIN STRUCTURES BASED ON SPATIAL PREFERENCE OF RESIDUES der

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Pretreatment and analysis techniques development of TKIs in biological samples for pharmacokinetic studies and therapeutic drug monitoring

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Journal of Pharmaceutical Analysis 2024年第4期14卷 439-459页

作者： Lan Chen yuan Zhang Yi-Xin Zhang Wei-Lai Wang De-mei Sun Peng-Yun Li xue-Song Feng Yue Tan School of Pharmacy China Medical UniversityShenyang110122China Institute of Pharmacology and Toxicology Institution National Engineering Research Center for Strategic DrugsBeijing100850China Department of Gastroenterology Shengjing Hospital of China Medical UniversityShenyang110022China

Tyrosine kinase inhibitors(TKIs)have emerged as the first-line small molecule drugs in many cancer therapies,exerting their effects by impeding aberrant cell growth and proliferation through the modulation of tyrosine kinase-mediated signaling ***,there exists a substantial inter-individual variability in the concentrations of certain TKIs and their metabolites,which may render patients with compromised immune function susceptible to diverse infections despite receiving theoretically efficacious anticancer treatments,alongside other potential side effects or adverse ***,an urgent need exists for an up-to-date review concerning the biological matrices relevant to bioanalysis and the sampling methods,clinical pharmacokinetics,and therapeutic drug monitoring of different *** paper provides a comprehensive overview of the advancements in pretreatment methods,such as protein precipitation(PPT),liquid-liquid extraction(LLE),solid-phase extraction(SPE),micro-SPE(μ-SPE),magnetic SPE(MSPE),and vortex-assisted dispersive SPE(VA-DSPE)achieved since *** also highlights the latest analysis techniques such as newly developed high performance liquid chromatography(HPLC)and high-resolution mass spectrometry(HRMS)methods,capillary electrophoresis(CE),gas chromatography(GC),supercritical fluid chromatography(SFC)procedures,surface plasmon resonance(SPR)assays as well as novel nanoprobes-based biosensing *** addition,a comparison is made between the advantages and disadvantages of different approaches while presenting critical challenges and prospects in pharmacokinetic studies and therapeutic drug monitoring.

关键词： TKIs Microextraction technique HRMS methods Pharmacokinetic studies Therapeutic drug monitoring

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Inherently Chiral and Symmetrical Heteroatom-Doped Zigzag-Type Hydrocarbon Belts

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CCS Chemistry 2024年第5期6卷 1198-1210页

作者： xue-yuan Wang Xin-Yu Zhang Shuo Tong Qing-Hui Guo mei-Ling Tan Cheng-Jun Li mei-Xiang Wang Key Laboratory of Bioorganic Phosphorous Chemistry and Chemical Biology(Ministry of Education) Department of ChemistryTsinghua UniversityBeijing 100084

hydrocarbon belts due to their potential applications in carbon *** contrast,heteroatomembedded zigzag hydrocarbon belts as advanced design strategies with fascinating structures and desirable,unique properties have remained largely unexplored,and inherently chiral ones are ***,we report the synthesis of a diversity of symmetric and highly enantiopure inherently chiral O-and N-doped zigzag-type hydrocarbon belts starting from strainless macrocycles based on fjord-stitching *** readily available pertriflated resorcin[6]arene underwent partial hydrolyses and intramolecular S_(N)Ar reactions to form a calix[3](9H-xanthene)derivative and a prochiral half-belt *** transformations of calix[3](9H-xanthene)into C_(3v)-symmetric molecular belts were achieved by closing the rest of the fjords with triple intramolecular S_(N)Ar reactions and Yamamoto homo coupling reactions,as well as palladiumcatalyzed intermolecular acridination reactions with primary *** prochiral half-belt underwent enantioselective desymmetrizative mono-acridination to afford an inherently chiral N,O_(4)-bridged pseudo-belt with an enantiomeric excess(ee)value of>99%under the catalysis of Pd(OAc)_(2)and(R)-*** closure reactions of the pseudo-belt produced diverse heteroatom-inlayed zigzag-type hydrocarbon belts of inherent ***,we showed the unique cavity structures of the belts and demonstrated interesting chiroptic properties of the inherently enantiopure chiral *** research opens the door for the exploration of novel and sophisticated symmetric and inherently chiral molecular nanobelt structures with outstanding physical and chemical properties,as well as potential applications.

关键词： zigzag-type molecular belt heterocycle chiroptical property fjord-stitching strategy resorcinarene

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Tailoring sulfur-doped and nitrogen-defective carbon nitride nanosheets for efficient photo-controlled radical polymerizations

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Science China Chemistry 2024年第7期67卷 2325-2334页

作者： xue Li Meng Kai Xu yuan Huang Tao Cai Key Laboratory of Biomedical Polymers of Ministry of Education College of Chemistry and Molecular SciencesWuhan UniversityWuhan 430072China Wuhan University Shenzhen Research Institute Shenzhen 518057China

Despite the challenges that remain,the synergistic adjustment of various microstructures and photochemical parameters of graphitic carbon nitride(g-C_(3)N_(4))in photocatalytic reactions holds promises for improving catalytic efficiency and reducing energy ***,sulfur-doped and nitrogen-defective g-C_(3)N_(4)(n-SC_(3)N_(x))nanosheets were designed and elaborately *** resultant n-SC_(3)N_(x)possessed a precisely defined 2D layer structure with extensive porosity and incremental specific surface *** photoinduced electron transfer-reversible addition-fragmentation chain transfer(PET-RAFT)polymerization of vinyl monomers with low dispersity,excellent temporal control and high chain-end fidelity was achieved under mild blue light irradiation in a nondegassed *** to their ultrathin nanostructures with nitrogen defects and sulfur dopants,n-SC_(3)N_(x)was capable of catalyzing RAFT polymerization in aqueous solutions at significantly accelerated rates,which were nearly 8 times faster compared to bulk g-C_(3)N_(4).The ease of separation and efficient reusability in subsequent polymerizations was enabled by the heterogeneous nature of n-SC_(3)N_(x).The appeal of this approach was illustrated by the fact that utilizing a reusable and metal-free photocatalyst in aqueous environments allowed for the synthesis of polymers with molecular weight up to 300 kg mol^(-1) and a dispersity of 1.32.

关键词： graphitic carbon nitride nitrogen defects sulfur dopants PET-RAFT polymerization recyclability

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Numerical Analysis of Surcharge Effect on Stability and Interaction Mechanism of Slope-Pile-Footing System

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Journal of Earth Science 2024年第3期35卷 955-969页

作者： Chao Xu Lei xue yuan Cui Mengyang Zhai Key Laboratory of Shale Gas and Geoengineering Institute of Geology and GeophysicsChinese Academy of SciencesBeijing 100029China Innovation Academy for Earth Science Chinese Academy of SciencesBeijing 100029China College of Earth and Planetary Sciences University of Chinese Academy of SciencesBeijing 100049China Yellow River Laboratory Zhengzhou UniversityZhengzhou 450001China

To investigate the stability and interaction mechanism of the slope-pile-footing system under surcharge effects,the finite difference method(FDM)was adopted to analyze the response laws of the stability of the reinforced slope,evolution of the critical slip surface,stress characteristic of retaining structures,deformation and failure modes of the slope foundation and building footing under surcharge parameters,including the surcharge intensity,the surcharge position,and the surcharge *** results show that surcharge parameters significantly affect the stability and the deformation characteristics of the slope-pile-footing *** speaking,with the increasing surcharge intensity and the decreasing surcharge position and width,the deformation and failure mode of the system will gradually evolve in a direction that is harmful to its *** interaction mechanism of the slope-pile-footing system is further clarified as the load transfer of the building footing,the generation of the additional stress in the slope foundation,and the adjustment of pile bending moment due to the stress ***,the safety of anti-slide piles will determine the stability of the slope foundation and building *** findings are expected to provide guidance for the comprehensive development and utilization of filled slopes after reinforcement.

关键词： slope-pile-footing system slope stability interaction mechanism finite difference method geotechnical engineering safety engineering.

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