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Let-7a gene knockdown protects against cerebral ischemia/reperfusion injury

Neural Regeneration Research 2016年第2期11卷 262-269页

作者： Zhong-kun Wang fang-fang Liu yu Wang Xin-mei Jiang xue-fan yu Department of Neurology First Hospital Jilin University Department of Neurology Jilin Central Hospital Department of Hepatopancreatobiliary Surgery the Second Hospital of Jilin University

The micro RNA（mi RNA） let-7 was one of the first mi RNAs to be discovered, and is highly conserved and widely expressed among species. let-7 expression increases in brain tissue after cerebral ischemia/reperfusion injury; however, no studies have reported let-7 effects on nerve injury after cerebral ischemia/reperfusion injury. To investigate the effects of let-7 gene knockdown on cerebral ischemia/reperfusion injury, we established a rat model of cerebral ischemia/reperfusion injury. Quantitative reverse transcription-polymerase chain reaction demonstrated that 12 hours after cerebral ischemia/reperfusion injury, let-7 expression was up-regulated, peaked at 24 hours, and was still higher than that in control rats after 72 hours. Let-7 gene knockdown in rats suppressed microglial activation and inflammatory factor release, reduced neuronal apoptosis and infarct volume in brain tissue after cerebral ischemia/reperfusion injury. Western blot assays and luciferase assays revealed that mitogen-activated protein kinase phosphatase-1（MKP1） is a direct target of let-7. Let-7 enhanced phosphorylated p38 mitogen-activated protein kinase（MAPK） and c-Jun N-terminal kinase（JNK） expression by down-regulating MKP1. These findings suggest that knockdown of let-7 inhibited the activation of p38 MAPK and JNK signaling pathways by up-regulating MKP1 expression, reduced apoptosis and the inflammatory reaction, and exerted a neuroprotective effect following cerebral ischemia/reperfusion injury.

关键词： nerve regeneration cerebral ischemia/reperfusion injury let-7 mitogen-activated protein kinase phosphatase-1 apoptosis microglia inflammation mitogen-activated protein kinase neurons c-Jun N-terminal kinase gene knockdown brain injury neural regeneration

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Neuronal intranuclear inclusion disease mimicking acute cerebellitis:A case report

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World Journal of Clinical Cases 2020年第23期8卷 6122-6129页

作者： Jiao-Jiao Guo Zi-Yi Wang Meng Wang Zong-Zhi Jiang xue-fan yu Department of Neurology and Neuroscience Center The First Hospital of Jilin UniversityChangchun 130021Jilin ProvinceChina

BACKGROUND Neuronal intranuclear inclusion disease(NIID)is an unusual autosomal dominant,chronic progressive neurodegenerative *** clinical manifestations of NIID are complex and varied,complicating its clinical *** the best of our knowledge,this report is the first to document sporadic adult-onset NIID mimicking acute cerebellitis(AC)that was finally diagnosed by imaging studies,skin biopsy,and genetic *** SUMMARY A 63-year-old man presented with fever,gait unsteadiness,dysarthria,and an episode of *** serum levels of white blood cells and C-reactive protein were significantly elevated.T2-weighted brain magnetic resonance imaging and fluid attenuation inversion recovery sequences showed bilateral high-intensity signals in the medial part of the cerebellar hemisphere beside the *** we initially considered a diagnosis of AC,the patient’s symptoms improved significantly without special treatment,prompting our consideration of ***-weighted imaging showed hyperintensity in the corticomedullary *** biopsy revealed eosinophilic inclusions positive for anti-p62 in epithelial sweat-gland *** repeat expansions in the Notch 2 N-terminal like C gene confirmed the diagnosis of *** For patients with clinical manifestations mimicking AC,the possibility of underlying NIID should be considered along with prompt rigorous examinations.

关键词： Neuronal intranuclear inclusion disease Acute cerebellitis Skin biopsy Genetic testing Magnetic resonance imaging Case report

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The Prognostic Significance of a Combined Determination of Cathepsin D and Estrogen Receptors in Breast Carcinomas with Positive Axillary Lymph Nodes

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Chinese Journal of Clinical Oncology 2006年第3期3卷 172-175,180页

作者： yun Niu xue Yang yu fan Ajuan Lu Tieju Liu Xilin Fu Department of Breast Pathology and Research Tianjin Medical University Cancer Institute and Hospital Tianjin 30006China.

OBJECTIVE The aim of this study was to investigate the correlation between cathepsin D （Cath-D） and estrogen receptor （ER）expression in breast cancer tissue and to explore the prognostic significance of their combined determination in breast carcinoma patients with positive axillary lymph nodes. METHODS One hundred and thirty-eight cases of breast carcinoma were examined by immunohistochemistry （IHC） and the results relating to patient follow-up analyzed. RESULTS The overall 5-year disease-free survival rate （DFS） was 60.9% （84/138） in the series. The positive rate of Cath-D expression in the tumor cells was 55.07% and the positive ER staining was 51.4%. A definite significant negative correlation was found between the positive rates for Cath-D and ER （r=-0.294, P=0.001） The Cath-D expression for the cases in clinical Stage Ⅱ, ≥10 positive-node and recurrence or distant metastasis, was higher than that those cases in clinical Stage II with fewer node-metastasis and with 5 year DFS （X^2=13.926, P=0.000; X^2=13.070, P=0.001; X^2=10.545, P=0.001）. However, there was no significant difference of Cath-D expression between 2 groups of patients with different ages or among the different histopathologic types of the nonspecific invasive carcinoma. In the combined examination of Cath-D and ER, the cases that were ER （＋） and Cath-D （-） had the highest 5-year DFS compared to other situations. In contrast, the cases that were reversed in expression, ie, ER（-） and Cath-D（＋）, had a lower 5-year DFS. There was a significant difference between the 2 conditions （X2=18.675, P=0.000）. CONCLUSION A combined determination and analysis of Cath-D and ER expression may be more useful to establish a prognosis than the biological characteristics of carcinomas with positive lymph nodes.

关键词： breast carcinoma cathepsin-D estrogen receptor combined determination prognosis.

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A Novel Energy-regenerative Active Suspension for Vehicles

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Journal of Shanghai Jiaotong university(Science) 2008年第2期13卷 184-188页

作者：郑雪春喻凡张勇超 State Key Laboratory of Mechanical System and Vibration Institute of Automotive EngineeringShanghai Jiaotong University

In order to regenerate electric power from the vibration excited by road unevenness,a novel energy- regenerative active suspension for vehicles was proposed with the description of its structure and its working principle with two modes switched in different operating ***,the novel active system was modeled and simulated to show the performance improvement in ride comfort in its electrical motor ***,the performance tests of the actuator prototype were carried out,which proves its capability for damping in its regenerative braking *** research results can provide useful guidance for the similar electrical active suspension design and development.

关键词： active suspension control algorithm energy regeneration vehicle dynamics

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Comparative Proteomics Study on Human High-metastatic Large Cell Lung Cancer Cell Lines with Differential Metastatic Potential

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中国肺癌杂志 2009年第6期12卷 521-522页

作者： Xingyang xue yu fan Li MA Jun CHEN Sen WEI Zhigang LI Hongyu LIU Haisu WAN Zhihao WU Qinghua ZHOU Tianjin Key Laboratory o fLung Cancer Metastasis and Tumor Microenvironment Tianjin Lung Cancer Institute Tianjin Medical University Hospital Tianjin 300052 China

Background and Objective Lung cancer is not only the most dangerous threating tumor to humans health and life, but also a malignant tumor with poor prognosis. It has been

关键词：肺癌癌细胞治疗疗效

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Observation of acoustic hybrid topological phases induced by the p-d orbital interactions

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Science China(Physics,Mechanics & Astronomy) 2025年第1期Mechanics & Astronomy) .卷 132-139页

作者： Xiao Xiang Feng Gao yu-Gui Peng Peng Wu Zong-Lin Li xue-Feng Zhu School of Physics and Innovation Institute Huazhong University of Science and Technology

In spinless systems,growing attention has recently been attracted to synthetic gauge fields,which redefine the fundamental crystal symmetries by utilizing the projective algebraic ***,synthetic gauge fields have been commonly investigated in single orbital systems,and explorations on hybrid orbitals remain elusive in ***,we report the experimental observation of hybrid topological phases induced by hybrid synthetic gauge flux,which is formed by the interaction between p and d orbitals embedded in acoustic *** breaking the translation symmetries of Lxand/or Ly,we unambiguously demonstrate the first-order M?bius edge states and higher-order corner *** work establishes a platform for seeking exotic topological phases induced by hybrid-orbitals interactions and initializing the framework of hybrid-orbitalsrelated topological *** applications can be anticipated in scenarios such as high-quality sensing and robust wave trapping due to the stepwise energy distribution of the hybrid topological phase.

关键词： topological insulator synthetic gauge field projective symmetry corner states

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不完全竞争下我国铜进口贸易市场势力测度及影响因素研究

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商业研究 2017年第7期 127-134页

作者：朱学红樊玉林谌金宇中南大学商学院长沙410083

中国在国际有色金属进口市场拥有较大市场份额,却缺乏定价能力。本文选取10个主要铜进口市场为研究对象,采用修正的SMR模型对2000-2015年间中国有色金属进口贸易的国际市场势力进行了测度,考察影响国际市场势力的主要因素。结果显示:中... 详细信息

中国在国际有色金属进口市场拥有较大市场份额,却缺乏定价能力。本文选取10个主要铜进口市场为研究对象,采用修正的SMR模型对2000-2015年间中国有色金属进口贸易的国际市场势力进行了测度,考察影响国际市场势力的主要因素。结果显示:中国铜进口市场是典型的非完全竞争市场,铜进口贸易不具有买方市场势力同时面临强大的卖方市场势力;在十个主要进口市场中,加拿大、美国、智利、秘鲁、澳大利亚等五个国家对出口价格具有额外加成能力,并依次占据卖方市场势力前五位;较高的进口依存度、进口结构单一以及较低的产业集中度是导致中国铜进口贸易买方国际市场势力缺失的主要原因。据此,我国应通过优化进口结构、加强对外投资、提高行业集中度来增强在铜资源国际进口贸易中的市场势力与定价话语权。

关键词：铜矿产资源剩余需求-供给市场势力定价权

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Influence of introducing Zr,Ti,Nb and Ce elements on externally solidified crystals and mechanical properties of high-pressure die-casting Al–Si alloy

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International Journal of Minerals,Metallurgy and Materials 2025年第1期32卷 147-153页

作者： Junjie Li Wenbo yu Zhenyu Sun Weichen Zheng Liangwei Zhang Yanling xue Wenning Liu Shoumei Xiong Center of Materials Science and Engineering School of Mechanical and Electronic Control EngineeringBeijing Jiaotong UniversityBeijing 100044China Traffic&Transportation Engineering Central South UniversityChangsha 410075China Shanghai Synchrotron Radiation Facility Shanghai Advanced Research InstituteChinese Academy of SciencesShanghai 201204China School of Materials Science and Engineering Tsinghua UniversityBeijing 100084China

High pressure die casting(HPDC)AlSi10Mn Mg alloy castings are widely used in the automobile *** can optimize the mechanical properties of castings through heat treatment,while the release of thermal stress arouses the deformation of large integrated ***,the development of non-heat treatment Al alloys is becoming the hot *** addition,HPDC contains externally solidified crystals(ESCs),which are detrimental to the mechanical properties of *** achieve high strength and toughness of non-heat treatment die-casting Al-Si alloy,we used AlSi9Mn alloy as matrix with the introduction of Zr,Ti,Nb,and *** influences on ESCs and mechanical properties were systematically investigated through three-dimensional reconstruction and thermodynamic *** results reveal that the addition of Ti increased ESCs'size and porosity,while the introduction of Nb refined ESCs and decreased ***,large-sized Al_3(Zr,Ti)phases formed and degraded the mechanical *** introduction of Ce resulted in the poisoning effect and reduced mechanical properties.

关键词： aluminium alloy high-pressure die-casting externally solidified crystals non-heat treatment

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三维设计软件模型数据交换研究

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黑龙江水利科技 2013年第10期41卷 13-16页

作者：樊雪钰黑龙江省水利水电勘测设计研究院哈尔滨150080

不同的三维设计软件默认的数据格式不同,因此三维协同数据交换时需要对格式进行转化,文中以UG NX与Bentley为例,探讨了STL、IGES、STEP与parasoild常见格式的特点,并对几种数据格式交换方式进行了比较。

关键词：三维协同数据格式数据交换数据完整性一致性推符格式

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Activation of the AMPK-ULK1 pathway plays an important role in autophagy during prion infection

Activation of the AMPK-ULK1 pathway plays an important role ...

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第十届全国免疫学学术大会

作者： Hui Wang xue-yu fan Chan Tian Lu-Bin Zhang Yang-Jing Zhao Qi-Xiang Shao Xiao-Ping Dong 江苏大学中国疾病预防控制中心

Objective: AMPK is a serine/threonine protein kinase that acts as a positive regulator of autophagy by phosphorylating ULK1 at specific sites. A previous study demonstrated activation of the macroautophagic system in scrapie-infected experimental rodents and in certain human prion diseases, in which the essential negative regulator MTOR is severely inhibited. In this study, we investigate the regulation of AMPK-ULK1 signal pathway in prion ***: Using brain homogenates infected with scrapie strain 263 K and scrapie-infected cell line SMB-S15 to observe potential changes of the endogenous kinase AMPK and ULK1 by western blot approach, then paraffin sections of cortex and cerebellum from normal and 263K-infected hamsters were employed to further investigate alteration and distribution of AMPK and ULK1 through Immunohistochemistry and Immunofluorescence techniques. Moreover, SMB-S15 cells were transiently transfected with the ULK1-specific si RNA to observe its effect on induced ***: Marked up-regulation of AMPK and AMPK-Thr172, ULK1 and ULK1-Ser555 were observed in terminal stage of brain homogenates infected with scrapie strain 263 K. Increases in brain AMPK and ULK1 occurred at an early stage of infection. The level of phosphorylated ULK1-Ser757 decreased during mid-infection and was only negligibly present at the terminal stage, a pattern that showed a close relationship of the phosphorylated protein with altered endogenous MTOR. In addition, the level of LKB1 associated with AMPK activation was selectively increased at the early and middle stages of infection. Knockdown of endogenous ULK1 in SMB-S15 cells inhibited LC3 ***: In addition to the abolishment of the MTOR regulatory pathway, activation of the AMPK-ULK1 pathway during prion infection contributes to autophagy activation in prion-infected brain tissues.

关键词： AMPK ULK1 autophagy prion scrapie mTOR LC3

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