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Role of thioredoxin-interacting protein in mediating endothelial dysfunction in hypertension

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Genes & Diseases 2022年第3期9卷 753-765页

作者： Ruiyu Wang Yongzheng Guo Lingjiao Li Minghao Luo Linqian Peng Dingyi Lv zhe Cheng Qian xue Liang Wang Jing Huang Department of Cardiology The Second Affiliated Hospital of Chongqing Medical UniversityChongqing 400010PR China Department of Cardiology The First Affiliated Hospital of Chongqing Medical UniversityChongqing 400016PR China Institute of Life Science Chongqing Medical UniversityChongqing 400016PR China

Excessive oxidative stress is a major causative factor of endothelial dysfunction in *** an endogenous pro-oxidant,thioredoxin-interacting protein(TXNIP)contributes to oxidative damage in various *** present study aimed to investigate the role of TXNIP in mediating endothelial dysfunction in *** vivo,an experimental model of acquired hypertension was established with two-kidney,one-clip(2K1C)*** expression of TXNIP in the vascular endothelial cells of multiple vessels was significantly increased in hypertensive rats compared with sham-operated ***,a TXNIP inhibitor,suppressed vascular oxidative damage and increased the expression and activity of eNOS in the aorta of hypertensive ***,impaired endothelium-dependent vasodilation was effectively improved by TXNIP inhibition in hypertensive *** vitro,we observed that Ang II increased the expression of TXNIP in primary human aortic endothelial cells(HAECs)and that TXNIP knockdown by RNA interference alleviated cellular oxidative stress damage and mitigated the impaired eNOS activation and intracellular nitric oxide(NO)production observed in Ang Il-treated ***,inhibiting thioredoxin(TRX)with PX-12 completely blunted the protective effect of silencing *** addition,TXNIP knockdown facilitated TRX expression and promoted TRX nuclear translocation to further activate AP1 and *** overexpressi on exhibited favorable effects on eNOS/NO homeostasis in Ang 11-treated ***,TXNIP contributes to oxidative stress and endothelial dysfunction in hypertension,and these effects are dependent on the antioxidant capacity of TRX,suggesting that targeting TXNIP may be a novel strategy for antihypertensive therapy.

关键词： Endothelial dysfunction eNOS Hypertension Oxidative stress Thioredoxininteracting protein

A proteome-wide screen identifies valosin-containing protein as an essential regulator of podocyte endoplasmic reticulum stress

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Chinese Science Bulletin 2012年第20期57卷 2493-2505页

作者： HUANG ZhiYong HONG Quan xue Peng PAUL Goulding FENG zhe WANG LiYuan MEI Yan WU LingLing CHEN XiangMei WU Di State Key Laboratory of Kidney Disease(2011DAV00088) Kidney Department&Institute of NephrologyDivision of Clinical Internal MedicineChinese People’s Liberation Army General HospitalBeijing 100853China Laboratory of Proteomics Institute of BiophysicsChinese Academy of SciencesBeijing 100101China Nonlinear Dynamics Ltd Keel HouseGarth HeadsNewcastle upon TyneNE12JEUK

To investigate proteins expressed in the renal tissue of the passive Heymann nephritis (pHN) rat model,we prepared pHN rat models with anti-FxA1 serum and analyzed the proteins differentially expressed in the kidney tissue with label-free liquid chromatography-tandem mass *** then analyzed in depth the endoplasmic reticulum stress (ERS)-related protein using an online bioinformatics ***-one differential proteins and their annotations were *** Ontology (GO) function analysis showed that 16 proteins were involved in cellular metabolism and 22 were proteins related to catalytic activity,including protein folding or ***-GO networks indicated that VCP could interact with the ERS marker HSPa5,with both involved in a single *** inhibition of podocyte VCP by RNAi under normal conditions,the HSPa5 expression level did not change,but when the cell was subjected to ERS by tunicamycin,HSPa5 expression significantly increased with RNAi of VCP when compared with the tunicamycin-treated *** results showed that ERS plays an important role in podocyte injury of membranous nephropathy and is mediated by an HSPa5-VCP signaling pathway,in which the most predominant proteins are those related to cellular metabolism and catalytic activity.

关键词：蛋白质折叠内质网应激细胞损伤 Networks公司宽屏幕标识大鼠模型催化活性

Nanoscale domain switching mechanism of Bi_(3.15)Eu_(0.85)Ti_3O_(12) thin film under the different mechanical forces

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Chinese Physics B 2015年第10期24卷 496-500页

作者：朱哲陈玉博郑学军 School of Materials Science and Engineering Xiangtan University School of Mechanical Engineering Xiangtan University

The switching process of ferroelectric thin films in electronic devices is one of the most important requirements for their application. Especially for the different external fields acting on the film surface, the mechanism of domain switching is more complicated. Here we observe the nanoscale domain switchings of Bi3.15Eu0.85Ti3O12 thin film under different mechanical forces at a fast scan rate. As the force increases from initial state to 247.5 n N, the original bright or grey contrasts within the selected grains are all changed into dark contrasts corresponding to the polarization vectors reversed from the up state to the down state, except for the clusters. As the mechanical force increases to 495 n N, the color contrasts in all of the selected grains further turn into grey contrasts and some are even changed into grey contrasts completely showing the typical 90° domain switching. When another stronger loading force 742.5 n N is applied, the phase image becomes unclear and it indicates that the piezoelectric signal can be suppressed under a sufficiently high force, which is coincident with previous experimental results. Furthermore, we adopt the domain switching criterion from the perspective of equilibrium state free energy of ferroelectric nanodomain to explain the mechanisms of force-generated domain switchings.

关键词： thin film domain switching mechanical force piezoresponse force microscopy

Complete Human Immunodeficiency Virus-1 Specific T Lymphocyte Response to Chinese Human Immunodeficiency Virus-1 B/C Chronic Infectors

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Biomedical and Environmental Sciences 2009年第6期22卷 522-528页

作者： zhe WANG HONG-WEI LIU KUN-xue HONG ZU-JIANG YU JIAN -PING CHEN YU-HUA RUAN QUAN-CHENG KAN YI-MING SHAO The First Affiliated Hospital of zhengzhou University Zhengzhou 450052 Henan China Center for AIDS/STD Control and Prevention Henan Center for Disease Control and Prevention Zhengzhou 450016 Henan China Division of Virology and Immunology National Center for Disease Control and Prevention Chinese Center for Disease Control and Prevention Beijing 100052 China

Objective To characterize the human immunodeficiency virus （HIV） -specific T lymphocyte responses and identify the immunodominant regions in Chinese HIV-1 recombinant subtype B/C chronic infectors at complete genome level. Methods Twenty-five HIV-I B/C recombinant chronic infectors were screened for their specific T lymphocyte responses to a panel of peptides corresponding to the complete HIV-1 subtype B genome by gamma interferon ELISPOT assay. Kruskal-Wallis nonparametric analysis of variance was used to test significant differences across gene regions, and Tukey pairwise analysis was used to identify differences between gene regions. Spearman rank correlation was used to assess the relation between responses. Results The order of recognized frequencies of specific T lymphocyte responses to HIV proteins was Nef〉Vpr〉Gag〉Pol〉Vpu〉Env〉Rev〉Vif〉Tat. When adjusted for protein length, Nef, Vpr, Gag, and Pol were the most intensely targeted proteins and the central region of Nef, Gag p24, Pol RT, and Vpr was most frequently recognized. No significant correlation was observed between the magnitude of IFN-γ production of HIV-l-specific T lymphocyte responses and plasma viremia, breadth of response and CD4 counts. Conclusion The central region of Nef, Gag p24, Pol RT, and Vpr is most frequently targeted in HIV-1 B/C recombinants chronic infectors. HIV-1-specific T lymphocyte responses and plasma viremia or CD4 counts play no protective role at complete genome level in these infectors.

关键词： Human immunodeficiency virus (HIV) Specific T lymphocyte response Elispot IFN-γ

Potentially predictive microRNAs of gastric cancer with metastasis to lymph node

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World Journal of Gastroenterology 2011年第31期17卷 3645-3651页

作者： Wen-Yi Wu Xiang-Yang xue zhe-Jing Chen Shao-Liang Han ying-Peng Huang Li-Fang Zhang Guan-Bao Zhu Xian Shen Department of General Surgery the First Affiliated Hospital of Wenzhou Medical CollegeWenzhou 325000Zhejiang ProvinceChina Department of microbiology Wenzhou Medical CollegeWenzhou 325000Zhejiang ProvinceChina

AIM:To detect the expression of 60 microRNAs(miRNAs)in gastric cancer tissues and find new predictive biomarkers of gastric cancer with ***:The expressions of 60 candidate miRNAs in 30 gastric cancer tissues and paired normal tissues were detected by stem-loop real-time reverse transcription-polymerase chain *** primary screening of miRNAs expression,5 selected miRNAs were further testified in another 22 paired gastric *** on the expression level of miRNAs and the status of metastasis to lymph node(LN),receiver-operating-characteristic(ROC)curve were used to evaluate their ability in predicting the status of metastasis to ***:Thirty-eight miRNAs expressions in gastric cancer tissues were significantly different from those in paired normal tissues(P<0.01).Among them,31miRNAs were found to be up-expressed in cancer tissues and 1 miRNAs were down-expressed≥1.5 fold vs paired normal gastric *** microRNAs(miR-125a-3p,miR-133b,miR-143,miR-195 and miR-212)were differently expressed between different metastatic groups in 30 gastric cancer biopsies(P<0.05).Partial correlation analysis showed that hsa-mir-212 and hsa-mir-195 were correlated with the status of metastasis to LN in spite of age,gender,tumor location,tumor size,depth of invasion and cell *** analysis indicated that miR-212 and miR-195 have better sensi-tivities(84.6%and 69.2%,respectively)and specifici-ties(both 100%)in distinguishing biopsies with metastasis to LN from biopsies without metastasis to ***:miR-212 and miR-195 could be independent biomarkers in predicting the gastric cancer with metastasis to LN.

关键词： MicroRNA miR-212 MiR-195 Gastric cancer Metastasis to lymph node

Kinetics and FTIR characteristics of the pyrolysis process of poplar wood

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Forestry Studies in China 2013年第1期15卷 70-75页

作者： Wen-Liang WANG xue-Yong REN Yan-zhe CHE Jian-Min CHANG Jin-Sheng GOU College of Materials Science and Technology Beijing Forestry University

The pyrolysis characteristics of residues of poplar （Populus sp.） wood were investigated using a thermogravimetric analyzer coupled with a Fourier transform infrared （TG-FTIR） spectrometer. The pyrolysis process was sub- divided into four stages at a rate of 10℃.min-1, varying from 30 to 650℃. Below 180℃, a mass loss occurred for drying and preheating the sample and the onset temperature of pyrolysis ranged between 180-260℃. A significant mass loss 3f 61.4 wt.% occurred between 260-380℃, followed by a slow and continuous mass change with lignin devolatilization. The analysis of kinetic reactions showed that the activation energy （78.29 ***-1） in the low-temperature section was much larger than that （6.40 kJ-mol-1） in the high-temperature section. The evolved gases formed by thermal degradation 3f poplar wood were simultaneously analyzed by FTIR. It was observed from the main peaks that the emissions evolved during poplar wood pyrolysis were acetic acid, carbon dioxide, carbon monoxide, methane, water, some volatile com- 3ounds of esters, alcohols and aldehydes. The emissions gradually increased with rising temperatures before a strong 3eak around 360℃ and then decreased. Most gaseous products were emitted in the 320-380℃ range, while CO2 was =ontinuously emitted in a wide range from 140-550℃.

关键词： poplar wood pyrolysis TG-FTIR kinetics characteristics

Leaching kinetics of selenium from copper anode slimes by nitric acid-sulfuric acid mixture

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Transactions of Nonferrous Metals Society of China 2018年第1期28卷 186-192页

作者： Hong-ying YANG xue-jiao LI Lin-lin TONG zhe-nan JIN Lu yin Guo-bao CHEN School of Metallurgy Northeastern University Shenyang 110819 China

The leaching kinetics of selenium from copper anode slimes was studied in a nitric acid?sulfuric acid *** effects of main parameters on selenium leaching showed that the leaching rate of selenium was practically independent of stirring speed,while dependent on temperature and the concentrations of HNO3and *** leaching of selenium includes two *** activation energy in the first stage is103.5kJ/mol,and the chemical reaction is the rate controlling *** was almost independent of H2SO4concentration and dependent on HNO3concentration since the empirical reaction order with respect to HNO3concentration *** the second stage,the activation energy is30.6kJ/mol,and the process is controlled by a mixture of diffusion and chemical *** leaching of selenium was almost independent of HNO3concentration.

关键词： selenium kinetics acid leaching copper anode slimes

Wildfire associated with a deciduous broadleaved forest from the Neogene Baoshan Basin at the southeastern margin of the Tibetan Plateau

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Journal of Palaeogeography 2023年第3期12卷 448-462页

作者： Yong-Jiang Huang Arata Momohara Shu-Feng Li xue-ping Ji Jian Qiu Lin-Bo Jia Jin-Jin Hu Yun-Heng Ji zhe-Kun Zhou Key Laboratory for Plant Diversity and Biogeography of East Asia Kunming Institute of BotanyChinese Academy of SciencesKunming 650201Yunnan ProvinceChina Graduate School of Horticulture Chiba UniversityChiba 271-8510Japan Key Laboratory of Tropical Forest Ecology Xishuangbanna Tropical Botanical GardenChinese Academy of SciencesMengla 666303Yunnan ProvinceChina Kunming Natural History Museum of Zoology Kunming Institute of ZoologyChinese Academy of SciencesKunming 650223Yunnan ProvinceChina School of Material and Chemical Engineering Southwest Forestry UniversityKunming 650224Yunnan ProvinceChina

Wildfire bears a close relationship with vegetation as its fuel *** southeastern margin of the Tibetan Plateau witnesses frequent wildfires among various types of vegetation,whereas such wide in-teractions between wildfire and vegetation remain poorly studied from geological *** this study,we reported a local fire using sedimentary macroscopic charcoals from the latest Miocene to early Pliocene of the Baoshan Basin in this region,and then inferred the local vegetation at the time of the fire event based chiefly on the coexistent fruit and seed fossil *** taxonomic results show that the charcoal assemblage is probably dominated by broadleaved plants and the fruit and seed fossil assemblage is apparently dominated by Salix(Salicaceae)followed by Sambucus(Adoxaceae),suggesting a deciduous broadleaved forest in which the fire likely *** a seasonally dry climate associated with the Asian monsoon,this type of vegetation might be prone to natural fire,because in the wet rainy season the plants grew well to accumulate biofuel and in the dry season abundant ground litter resulting from leaf decay would be desiccated to become highly *** to the fire-tolerant habit of Salix as the dominant plant,the forest might be in return adapted to the fire event or even more fires that potentially *** these may suggest a close relationship between the fire event and the reconstructed *** finding documents a new type of wildfire—vegetation interaction,namely the interaction between wildfire and deciduous broadleaved forest,from the geological past at the southeastern margin of the Tibetan *** therefore sheds new light on the wildfire history coupling vegetation change in the region.

关键词： Charcoal Fossil carpology Salix Sambucus Precipitation seasonality

Propofol inhibits neuronal differentiation of mouse embryonic stem cells in vitro

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Chinese Medical Journal 2013年第21期126卷 4186-4188页

作者： Wang Hao Luo zhe xue Zhang-gang Cang Jing Fudan Univ Shanghai Med Coll Zhongshan Hosp Dept Anesthesiol Shanghai 200032 Peoples R China

Propofol （2, 6-diisopropylphenol） is a general intravenous anesthetic which plays roles in the central neural system by binding GABAA receptors （GABAARs） and enhancing the chloride channels of the neurons.1 Previous studies mainly focused on the effects of anesthetics on mature neurons, but little attention was paid to their role in early neural differentiation or neural stem cells. Therefore, in the present study, we choose the widely used mouse embryonic cells （ES） cells as the model to investigate the potential effect ofpropofol on neuronal differentiation.

关键词： propofol neuronal differentiation mouse embryonic stem cells