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Synthesis of PEGylated hyaluronic acid for loading dichloro(1,2-diaminocyclohexane)platinum(Ⅱ)(DACHPt) in nanoparticles for cancer treatment

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Chinese Chemical Letters 2015年第6期26卷 695-699页

作者： Xiu-Quan Quan Lin Kang xue-zhe yin zhe-Hu Jin Zhong-Gao Gao State Key Laboratory of Bioactive Substance and Function of Natural Medicines Institute of Materia MedicaChinese Academy of Medical Sciences and Peking Union Medical College Yanbian University Hospital

Dichloro（1,2-diaminocyclohexane）platinum（ll）（DACHPt）, a cisplatin （CDDP） analog, has shown lower toxicity than CDDP and no cross-resistance with CDDP in many CDDP-resistant cancers. PEGylated hyaluronan （mPEG-HA） is an mPEG conjugated with hyaluronan biodegradable polymer which is a naturally occurring biopolymer in the interstitium, is primarily cleared by the lymphatic system, mPEG- hyaluronan-DACHPt （PEG-HA-Pt） conjugate could circulate long-term in the bloodstream and increase DACHPt concentration in the tumor site and decrease systemic toxicity, mPEG-HA conjugates with the range of 1%-5% substitution were synthesized, and the structures were confirmed by 1H NMR and IR. The particle size of DACHPt incorporated with mPEG-HA was about 86 nm and the loading content and efficiency were about 19% （w/w） and 86%, respectively. The synthesized mPEG-HA with different PEG substitution degrees presented non toxicity, and the cell viability of DACHPt loaded in mPEG-HA nanoparticles increased with increasing doses of DACHPt. DACHPt release from nanoparticles slightly decreased with increasing PEG substitution degree from 1% to 5% at 37℃, pH 7.4 PBS solution. The DACHPt loaded in mPEG-HA nanoparticles significantly inhibited the growth ofA549 xenografts in nude mice when compared to the DACHPt loaded in HA nanoparticles and the control group after 4 weeks treatment （p 〈 0.01 compared with control）. The body weight change curve shows that the mice weight loss was less than 5% by treating with both DACHPt loaded in mPEG-HA and HA nanoparticles. In conclusion, a novel DACHPt loaded mPEG-HA delivery system was developed with sustained release and increased platinum concentration in the tumor.

关键词： DACHPt PEGylated hyaluronan A549 lung cancer cell

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Effect of Boschniakia rossica on expression of GSTP,p53 and p21^(ras)proteins in early stage of chemical hepatocarcinogenesis and its anti-inflammatory activities in rats

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World Journal of Gastroenterology 2000年第6期6卷 812-818页

作者： Zong Zhu yin Hai Ling Jin xue zhe yin Tian Zhu Li Ji Shu Quan Zeng Nan Jin Institute for Cancer Research,Yanbian University College of Medicine,Yanji 133000,Jilin Province,China 延边大学吉林延边

AIM To investigate the effect of Boschniakiarossica（BR）extract on expression of GST-P,p53 and p21rasproteins in early stage of chemicalhepatocarcinogenesis in rats and its anti-inflammatory *** The expression of tumor marker-placental form glutathione S-transferase（GST-P）,p53 and p21rasproteins were investigated byimmunohistochemical techniques and ***-inflammatory activities of BR werestudied by xylene and croton oil-induced mouseear edema,carrageenin,histamine and hotscald-induced rat pow edema,adjuvant-inducedrat arthritis and cotton pellet-induced mousegranuloma formation *** The 500 mg/kg of BR-H2O extractfractionated from BR-Methanol extract hadinhibitory effect on the formation of DEN-inducedGST-P-positive foci in rat liver（GST-P stainingwas 78% positive in DEN+AAF group vs 20%positive in DEN+AAF+BR group,Prasproteinwas lower than that of hepatic preneoplasticlesions（33% and 22% positive respectively inDEN+AAF group vs negative in DEN+AAF+BRgroup）.Both CH2Cl2 and H2O extracts from BRhad anti-inflamatory effect in xylene and crotonoil-induced mouse ear edema（inhibitory rateswere 26%-29% and 35%-59%,respectively）. BR-H2O extract exhibited inhibitory effect incarrageenin,histamine and hot scald-inducedhind paw edema and adjuvant-induced arthritis inrats and cotton pellet-induced granulomaformation in *** BR extract exhibited inhibitory effect on formation of preneoplastic hepatic foci in early stage of rat chemical hepato-carcinogenesis. Both CH2CI2 and H2O extracts from BR exerted anti-inflammatory effect in rats and mice.

关键词： Boschniakia rossica liver neoplasms/chemically induced glutathione transferases protein p53 immunohistochemistry anti-inflammatory agents rats

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Dendroaspis natriuretic peptide relaxes gastric antral circular smooth muscle of guinea-pig through thecGMP/cGMP-dependent protein kinase pathway

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World Journal of Gastroenterology 2008年第35期14卷 5461-5466页

作者： Chun-Yu Cai zheng-Xu Cai Xin-Yi Gu Lu-Juan Shan Yong-Xiao Wang xue-zhe yin Qing-Hui Qi Hui-Shu Guo Department of Internal Medicine the Affiliated Hospital of Yanbian University Yanji 133000 Jilin Province China Department of Neurology the First Affiliated Hospital of Dalian Medical University Dalian 116011 Liaoning Province China Centralab Zhongshan Affiliated Hospital of Dalian University Dalian 116001 Liaoning Province China Centralab the First Affiliated Hospital of Dalian Medical University Dalian 116011 Liaoning Province China Center for Cardiovascular Sciences Albany Medical College Albany NY 12208 United States

AIM: To systematically investigate if cGMP/cGMP- dependent protein kinase G (PKG) signaling pathway may participate in dendroaspis natriuretic peptide (DNP)-induced relaxation of gastric circular smooth muscle. METHODS: The content of cGMP in guinea pig gastric antral smooth muscle tissue and perfusion solution were measured using radioimmunoassay; spontaneous contraction of gastric antral circular muscles recorded using a 4-channel physiograph; and Ca2+-activated K+ currents (IK(Ca)) and spontaneous transient outward currents (STOCs) in isolated gastric antral myocytes were recorded using the whole-cell patch clamp technique. RESULTS: DNP markedly enhanced cGMP levels in gastric antral smooth muscle tissue and in the perfusion medium. DNP induced relaxation in gastricantral circular smooth muscle, which was inhibited by KT5823, a cGMP-dependent PKG inhibitor. DNP increased IK(Ca). This effect was almost completely blocked by KT5823, and partially blocked by LY83583, an inhibitor of guanylate cyclase to change the production of cGMP. DNP also increased STOCs. The effect of DNP on STOCs was abolished in the presence of KT5823, but not affected by KT-5720, a PKA-specific inhibitor. CONCLUSION: DNP activates IK(Ca) and relaxes guinea-pig gastric antral circular smooth muscle via the cGMP/PKG-dependent singling axis instead of cAMP/ PKA pathway.

关键词： Dendroaspis natriuretic peptide Cyclic guanosine monophosphate Protein kinase G Proteinkinase A Gastric motility

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草苁蓉环烯醚萜苷对二乙基亚硝胺诱发肝癌大鼠细胞凋亡的影响

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中国现代医学杂志 2017年第27期27卷 7-11页

作者：崔香丹郑峰朱洁波全吉淑尹学哲延边大学附属医院吉林延吉133000 延边大学基础医学院吉林延吉133000

目的观察二乙基亚硝胺(DEN)诱发大鼠肝癌的病理变化,探讨草苁蓉环烯醚萜苷(IGBR)对细胞凋亡的作用及对p53、Bcl-2蛋白表达的影响。方法 132只Wistar雄性大鼠随机分为对照组、模型对照组、阳性对照组及IGBR组。除对照组,各组大鼠给予DEN ... 详细信息

目的观察二乙基亚硝胺(DEN)诱发大鼠肝癌的病理变化,探讨草苁蓉环烯醚萜苷(IGBR)对细胞凋亡的作用及对p53、Bcl-2蛋白表达的影响。方法 132只Wistar雄性大鼠随机分为对照组、模型对照组、阳性对照组及IGBR组。除对照组,各组大鼠给予DEN 0.2 g/kg腹腔注射1次,而后0.05%的DEN水溶液用于自由饮水;阳性对照组腹腔注射5-氟尿嘧啶(5-FU)0.025 g/kg每周3次;IGBR组每日IGBR 0.5 g/kg灌胃1次。实验第12、20及28周末,分批处死动物,观察肝脏病理变化及细胞凋亡,免疫组织化学法和Western blot检测p53、Bcl-2的表达。结果与对照组比较,模型对照组肝细胞核大且深染,肝细胞变性、异型增生,有些增生灶可见癌变细胞。凋亡的肝细胞皱缩、核固缩及核仁消失。IGBR组与阳性对照组凋亡指数高于模型对照组,差异有统计学意义(P0.05)。免疫组织化学结果,p53和Bcl-2阳性细胞主要分布于不典型增生灶和癌灶的胞浆中。与模型对照组比较,IGBR组和阳性对照组p53表达强度增强,而Bcl-2表达强度减弱,差异有统计学意义(P0.05)。结论大鼠肝癌发生与细胞凋亡相关,IGBR通过调控p53、Bcl-2来抑制DEN诱发大鼠肝癌。

关键词：肝癌草苁蓉环烯醚萜苷凋亡 p53 Bcl-2

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Area-efficient analog decoder design for low density parity check codes in deep-space applications

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The Journal of China Universities of Posts and Telecommunications 2017年第4期24卷 69-75页

作者： Zhao zhe Gao Fei zheng Hao yin xue School of Information and Electronics Beijing Institute of Technology Beijing 100081 China

Area-efficient design methodology is proposed for the analog decoding implementations of the rate-l/2 accumulate repeat-4 jagged-accumulate （AR4JA） low density parity check （LDPC） code. The proposed approach is designed using optimized decoding architecture and regularized routing network, in such a way that the overall wiring overhead is minimized and the silicon area utilization is significantly improved. The prototyping chip used to verily the approach is tully integrated in a four-metal double-poly 0.35 lam complementary metal oxide semiconductor （CMOS） technology, and includes an input-output interface that maximizes the decoder throughput. The decoding core area is 2.02 mm2 with a post-layout area utilization of 80%. The decoder was successfully tested at the maximum data rate of 10 Mbit/s, with a core power consumption of 6.78 mW at 3.3 V, which corresponds to an energy per decoded bit of 0.677 nJ. The proposed analog LDPC decoder with low processing power and high-reliability is suitable lbr space- and power-constrained spacecraft system.

关键词： low density parity check (LDPC) code analog decoding iterative message-passing algorithms hardware efficient area utilization

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Active flow control of S-duct by plasma synthetic jet

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Journal of zhejiang University-Science A(Applied Physics & Engineering) 2022年第8期23卷 652-668页

作者： Ru-bing LIU Xiao-yin MEI Sheng-hui xue Yu-wen LU zhe-zhe SU Qi LIN School of Aerospace Engineering Xiamen UniversityXiamen 361102China Fujian Key Laboratoryof Plasmaand Magnetic ResonanceResearch Xiamen 361102China

Flow separation and secondary flow in the S-duct of an aircraft engine cause severe pressure loss and airflow distortion at the outlet,lowering engine ***,a serial two-electrode plasma synthetic jet(PSJ)actuator array is used to actively control the flow field in the duct and improve its *** results show that the PSJ significantly increases the wall pressure recovery coefficient,suppresses flow separation,and improves the outlet pressure *** primary and secondary orders of the influencing factors are as follows:control position>jet momentum coefficient>excitation frequency>jet *** best jet control position is near the separation location,and the best jet configuration is the‘Λ’*** higher the jet momentum coefficient and excitation frequency,the better the flow *** wall pressure coefficient increases by up to 127.8%,and the outlet steady pressure distortion index decreases by 9.15%.The control mechanism is the direct energy injection into the flow boundary layer through a high-speed jet and the indirect control effect of the induced streamwise *** the one hand,the PSJ suppresses flow separation by improving the ability of the boundary layer to resist the inverse pressure *** the other hand,it reduces pressure distortion by decreasing the intensity of the secondary flow and weakening the *** study thus provides a new technology for the active control of the flow-field characteristics in an S-duct and has significance for guiding the application of synthetic jet technology in S-ducts.

关键词： S-duct Flow control Plasma synthetic jet(PSJ) Flow separation Pressure distortion

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Succession of Denitrifying Community Composition in Coastal Wetland Soils Along a Salinity Gradient

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Pedosphere 2012年第3期22卷 367-374页

作者： PIAO zhe ZHANG Wen-Wen MA Shuai LI Yu-Min yin Shi-xue Microbiology Section College of Environmental Science and EngineeringYangzhou UniversityYangzhou 225009(China)

To better understand the effect of salinity on denitrification communities, soils along a salinity gradient （ranging from 7.32 to 1.70 mS cm 1） in a wetland along the Yellow Sea coastline in Jiangsu Province, China, were studied using both culture-dependent and -independent methods. Culture efforts yielded 82 isolates in total, 81.7% of which were close relatives of Bacillus sp. based on partial sequences of their 16S rRNA genes. Denaturing gradient gel electrophoresis （DGGE） analysis based on 16S rRNA sequences suggested possible existence of bacterial community succession along the salinity gradient. Clone library analysis based on nosZ gene sequences （coding nitrous oxide reductase） showed that operational taxonomic units （OTUs） associated with α-proteobacteria dominated in all three soils, whereas those associated with β- and γ-subdivisions showed a clear succession. In the high salinity soil, only the OTUs associated with a-subdivision were found. In the medium salinity soil, small proportions of β- （6.5%） and γ-associated （19.6%） OTUs were found. In the low salinity soil, the proportions were further increased to 33% and 25% for β- and γ-Proteobacteria, respectively. Statistic analysis using Unifrac P test showed that nosZ-communities in different saline soils were significantly different from each other. It could be concluded that α-subdivision of nosZ-community tended to be sustained in high salinity environments whereas β and γ-subdivisions, especially the former, tended to be sustained in low salinity environments. Salinity was the key determinant of nosZ-community composition in the environment.

关键词： clone library denaturing gradient gel electrophoresis (DGGE) nosZ gene Yellow Sea coastline

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KLF4 Inhibits the Activation of Human Hepatic Stellate Cell In Vitro

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Current Medical Science 2024年第3期44卷 512-518页

作者： Xing-yu YANG zhe CHEN Jun TAN yin-kai xue Hai zheNG Department of Emergency Surgery Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430022China Department of Thoracic Surgery The First Affiliated Hospital of Xi’an Jiaotong UniversityXi’an710061China Department of Thoracic Surgery Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430022China

Objective Hepatic stellate cells(HSCs)play a crucial role in liver ***-stage liver fibrosis is reversible and intimately associated with the state of ***-like factor 4(KLF4)plays a pivotal role in a wide array of physiological and pathological *** study aimed to investigate the effect of KLF4 on the proliferation,apoptosis and phenotype of quiescent HSCs Methods We designed a KLF4 lentiviral vector and a KLF4 siRNA lentiviral vector,to upregulate and silence KLF4 expression in human HSC LX-2 cells via *** proliferation was assessed using the CCK-8 *** cytometry was used to detect the cell cycle distribution and apoptosis *** blotting was used to determine the levels of some quiescence and activation markers of HSCs Results Overexpression of KLF4 significantly increased the levels of E-cadherin and ZO-1,which are quiescent HSC markers,while significantly decreased the levels of N-cadherin and a-SMA,known activated HSC *** contrast,cell proliferation and apoptosis rates were elevated in LX-2 cells in which KLF4 expression was silenced Conclusion KLF4 inhibits the proliferation and activation of human LX-2 *** might be a key regulatory protein in the maintenance of HSC quiescence and may serve as a target for the inhibition of hepatic fibrosis.

关键词： Kruppel-like factor 4 hepatic stellate cells LX-2 cells liver fibrosis

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An Algorithm for the Discretization of an Ideal Projector

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Journal of Systems Science & Complexity 2016年第5期29卷 1400-1410页

作者： JIANG xue ZHANG Shugong LI zhe Institute of Mathematics Key Laboratory of Symbolic Computation and Knowledge Engineering (Ministry of Education) Jilin University Department of Applied Mathematics School of Science Changchun University of Science and Technology

Ideal interpolation is a generalization of the univariate Hermite interpolation. It is well known that every univariate Hermite interpolant is a pointwise limit of some Lagrange ***, a counterexample provided by Shekhtman Boris shows that, for more than two variables,there exist ideal interpolants that are not the limit of any Lagrange interpolants. So it is natural to consider: Given an ideal interpolant, how to find a sequence of Lagrange interpolants(if any) that converge to it. The authors call this problem the discretization for ideal interpolation. This paper presents an algorithm to solve the discretization problem. If the algorithm returns "True", the authors get a set of pairwise distinct points such that the corresponding Lagrange interpolants converge to the given ideal interpolant.

关键词： Discretization ideal interpolation Lagrange interpolation.

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A nested partitioning-based solution method for seru scheduling problem with resource allocation

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Journal of Management Science and Engineering 2024年第1期9卷 101-114页

作者： zhe Zhang Kazuhiro Izui Xiaoling Song Yong yin xue Gong School Economics&Management Nanjing University of Science and TechnologyNanjing210094China Department of Mechanical Engineering and Science Kyoto UniversityKyoto615-8540Japan Graduate School of Business Doshisha UniversityKarasuma-ImadegawaKamigyo-kuKyoto602-8580Japan

This paper investigates the production scheduling problems of allocating resources and sequencing jobs in the seru production system(SPS).As a new-type manufacturing mode arising from Japanese production practices,seru production can achieve efficiency,flexibility,and responsiveness *** production environment in which a set of jobs must be scheduled over a set of serus according to due date and different execution modes is considered,and a combination optimization model is *** by the problem complexity and the characteristics of the proposed seru scheduling model,a nested partitioning method(NPM)is designed as the solution ***,computational studies are conducted,and the practicability of the proposed seru scheduling model is ***,the efficiency of the nested partitioning solution method is demonstrated by the computational results obtained from different scenarios,and the good scalability of the proposed approach is proven via comparative analysis.

关键词： Seru production system Scheduling Combination optimization model Nested partitioning method Resource allocation

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