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Correlation study of biological characteristics of non-small cell lung cancer A549 cells after transfecting plasmid by microbubble ultrasound contrast agent

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Asian Pacific Journal of Tropical Medicine 2016年第6期9卷 564-567页

作者： Xuan-Yan Guo Man-Lu xue-qing chen Fan-Ding He Ang-Li Ultrasonic Department Academy of Sciences of Sichuan Province People's Hospital of Sichuan Province Pancreatic Surgery West China Hospital Sichuan University

Objective: To explore the role of the abnormal expression of miRNAs in the development process of non-small cell lung cancer and the feasibility of ultrasound microbubble-mediated gene therapy after transfecting antisense miRNA-224 and miRNA-122 a plasmids into nonsmall cell lung cancer A549 cells. Methods: Antisense miRNA-224 and miRNA-122 a plasmids were transfected into non-small cell lung cancer A549 cells on the optimal ultrasound microbubble mediated condition. We set up a control group. The cell proliferation activity, apoptosis, invasion ability were detected by MTT assay, Annexin V-PE, Transwell invasion experiment and colony formation assay, respectively. Results: The expression of mi RNA-224 decreased and the expression of miRNA-122 a rose after the plasmids of target genes were transfected into non-small cell lung cancer A549 cells, and there were significant differences when compared with those of the control group(P<0.05). After the plasmids of target genes were transfected into A549 cells, the growth of antisense miRNA-224 and miRNA-122 a were inhibited, and the differences were significant as compared with the control group(P < 0.05). Besides, the inhibition of miRNA-122 a group was the most significant and there was statistically significant difference as compared with miRNA-224 group(t =-4.694, P = 0.009). After the plasmids of target genes were transfected into A549 cells, the proportion of apoptotic cells increased, the invasive cells were decreased and the clone ability reduced, and also there was a significant difference as compared with those of the control group(P < 0.05). What's more, the apoptotic peak appeared in miRNA-122 a group. Its invasion ability decreased most obviously(40.25 ± 3.97/visual field), the number of clone ability was 104.93 ± 4.87 and the inhibitory effect was the most obviously. There was statistically significant difference as compared with other groups(P < 0.05). Conclusions: A549 cells transfected by ultrasound micro

关键词： Microbubble ultrasound contrast agent MiR-224 MiR-122a Non-small cell lung cancer

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The Role of HIV Replicative Fitness in Perinatal Transmission of HIV

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Virologica Sinica 2011年第3期26卷 147-155页

作者： xue-qing chen Chang Liu Xiao-hong Kong （Laboratory of Medical Molecular Virology School of Medicine Nankai University Tianjin 300071 China）

Perinatal transmission of Human immunodeficiency virus(HIV),also called mother-to-child transmission(MTCT),accounts for 90% of infections in infants worldwide and occurs in 30%-45% of children born to untreated HIV-1 infected *** HIV-1 infected mothers,some viruses are transmitted from mothers to their infants while others are *** relationship between virologic properties and the pathogenesis caused by HIV-1 remains *** studies have demonstrated that one obvious source of selective pressure in the perinatal transmission of HIV-1 is maternal neutralizing *** studies have shown that viruses which are successfully transmitted to the child have growth advantages over those not transmitted,when those two viruses are grown ***,the higher fitness is determined by the gp120 protein of the virus *** suggests that the selective transmission of viruses with higher fitness occurred exclusively,regardless of transmission *** are many factors contributing to the selective transmission and HIV replicative fitness is an important one that should not be *** review summarizes current knowledge of the role of HIV replicative fitness in HIV MTCT transmission and the determinants of viral fitness upon MTCT.

关键词： Human immunodeficiency virus(HIV) Acquired immune deficiency syndrome(AIDS) Mother-to-child transmission(MTCT) Replicative fitness Gp120

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Expression of early growth response factor-1 in rats with cerulein-induced acute pancreatitis and its significance

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World Journal of Gastroenterology 2005年第32期11卷 5022-5024页

作者： Lan-Bo Gong Li He Yang Liu xue-qing chen Bo Jiang Department of Gastroenterology Nanfang Hospital Southern Medical University Guangzhou 510515 Guangdong Province China Department of Gastroenterology Second Affiliated Hospital of Shenyang Medical College Shenyang 110002 Liaoning Province China

AIM： To observe the expressions of early growth response factor-1 （Egr-1） and tissue factor （TF） in rats with cerulein-induced acute pancreatitis and to explore its significance. METHODS： A large dose of cerulein was used to create the experimental acute pancreatitis model in rats. The changes of Egr-1 mRNA and protein in rats were observed during 30 min to 4 h after the treatment and immunohistochemical method was used to observe the localized expression of Egr-1 in tissues. In addition to the mRNA expression of Egr-1 target gene, TF was also observed. A blank control group, and a bombesinadministered group were used for comparison. RESULTS： Alter the stimulation of a large dose of cerulein, the rats showed typical inflammatory changes of acute pancreatitis. Thirty minutes alter the stimulation, the mRNA expression of Egr-1 in the pancreatic tissue reached its peak and then declined, while the expression of Egr-1 protein reached its peak 2 h after the stimulation. Histologically, 2 h after the stimulation, almost all pancreatic acinar cells had the expression of Egr-1 protein, which was focused in the nuclei. The mRNA expression of TF occurred 1 h after the stimulation and gradually increased within 4 h. However, a large dose of bombesin only stimulated the pancreatic tissue to produce a little mRNA expression of Egr-1 and no mRNA expression of Egr-1 protein and TF. CONCLUSION： Egr-1 as a pro-inflammatory transcription factor may play an important role in the pathogenesis of acute pancreatitis by modulating the expression of TF.

关键词： Growth response factor-1 Tissue factor Acute pancreatitis Cerulein Bombesin

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Targeting cholesterol trafficking to mitigate axonal degeneration in hereditary spastic paraplegia

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Neural Regeneration Research 2025年第5期20卷 1397-1398页

作者： Zhenyu chen xue-Jun Li Department of Biomedical Sciences University of Illinois College of Medicine RockfordRockfordILUSA Department of Bioengineering University of Illinois at ChicagoChicagoILUSA

Axonal degeneration underlies many debilitating diseases including hereditary spastic paraplegia(HSP),a genetically and clinically diverse group of disorders characterized by spasticity and weakness of the lower *** is one significant cause of chronic neurodisability due to the lack of effective treatments and a wide range of onset ages from early childhood to 70 years.

关键词： degeneration diseases hereditary

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The complex roles of m^(6)A modifications in neural stem cell proliferation, differentiation, and self-renewal and implications for memory and neurodegenerative diseases

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Neural Regeneration Research 2025年第6期20卷 1582-1598页

作者： Yanxi Li Jing xue Yuejia Ma Ke Ye xue Zhao Fangliang Ge Feifei Zheng Lulu Liu Xu Gao Dayong Wang qing Xia Department of Biochemistry and Molecular Biology College of Basic Medical SciencesHarbin Medical UniversityHarbinHeilongjiang ProvinceChina College of Basic Medical Sciences Harbin Medical UniversityHarbinHeilongjiang ProvinceChina Basic Medical Institute Heilongjiang Academy of Medical SciencesHarbinHeilongjiang ProvinceChina Key Laboratory of Heilongjiang Province for Genetically Modified Animals Harbin Medical UniversityHarbinHeilongjiang ProvinceChina Translational Medicine Research and Cooperation Center of Northern China Heilongjiang Academy of Medical SciencesHarbinHeilongjiang ProvinceChina Dongzhimen Hospital Beijing University of Chinese MedicineBeijingChina

N6-methyladenosine(m^(6)A), the most prevalent and conserved RNA modification in eukaryotic cells, profoundly influences virtually all aspects of mRNA metabolism. mRNA plays crucial roles in neural stem cell genesis and neural regeneration, where it is highly concentrated and actively involved in these processes. Changes in m^(6)A modification levels and the expression levels of related enzymatic proteins can lead to neurological dysfunction and contribute to the development of neurological diseases. Furthermore, the proliferation and differentiation of neural stem cells, as well as nerve regeneration, are intimately linked to memory function and neurodegenerative diseases. This paper presents a comprehensive review of the roles of m^(6)A in neural stem cell proliferation, differentiation, and self-renewal, as well as its implications in memory and neurodegenerative diseases. m^(6)A has demonstrated divergent effects on the proliferation and differentiation of neural stem cells. These observed contradictions may arise from the time-specific nature of m^(6)A and its differential impact on neural stem cells across various stages of development. Similarly, the diverse effects of m^(6)A on distinct types of memory could be attributed to the involvement of specific brain regions in memory formation and recall. Inconsistencies in m^(6)A levels across different models of neurodegenerative disease, particularly Alzheimer's disease and Parkinson's disease, suggest that these disparities are linked to variations in the affected brain regions. Notably, the opposing changes in m^(6)A levels observed in Parkinson's disease models exposed to manganese compared to normal Parkinson's disease models further underscore the complexity of m^(6)A's role in neurodegenerative processes. The roles of m^(6)A in neural stem cell proliferation, differentiation, and self-renewal, and its implications in memory and neurodegenerative diseases, appear contradictory. These inconsistencies may be a

关键词： Alzheimer's disease cell self-renewal central nervous system memory microglia nerve regeneration neurodegenerative diseases neurogenesis RNA methylation

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Potassium and calcium channels in different nerve cells act as therapeutic targets in neurological disorders

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Neural Regeneration Research 2025年第5期20卷 1258-1276页

作者： qing Qiu Mengting Yang Danfeng Gong Haiying Liang Tingting chen Department of Pharmacology School of PharmacyNantong UniversityNantongJiangsu ProvinceChina Jiangsu Province Key Laboratory of Inflammation and Molecular Drug Target NantongJiangsu ProvinceChina Department of Pharmacy Longyan First Affiliated Hospital of Fujian Medical UniversityLongyanFujian ProvinceChina

The central nervous system, information integration center of the body, is mainly composed of neurons and glial cells. The neuron is one of the most basic and important structural and functional units of the central nervous system, with sensory stimulation and excitation conduction functions. Astrocytes and microglia belong to the glial cell family, which is the main source of cytokines and represents the main defense system of the central nervous system. Nerve cells undergo neurotransmission or gliotransmission, which regulates neuronal activity via the ion channels, receptors, or transporters expressed on nerve cell membranes. Ion channels, composed of large transmembrane proteins, play crucial roles in maintaining nerve cell homeostasis. These channels are also important for control of the membrane potential and in the secretion of neurotransmitters. A variety of cellular functions and life activities, including functional regulation of the central nervous system, the generation and conduction of nerve excitation, the occurrence of receptor potential, heart pulsation, smooth muscle peristalsis, skeletal muscle contraction, and hormone secretion, are closely related to ion channels associated with passive transmembrane transport. Two types of ion channels in the central nervous system, potassium channels and calcium channels, are closely related to various neurological disorders, including Alzheimer's disease, Parkinson's disease, and epilepsy. Accordingly, various drugs that can affect these ion channels have been explored deeply to provide new directions for the treatment of these neurological disorders. In this review, we focus on the functions of potassium and calcium ion channels in different nerve cells and their involvement in neurological disorders such as Parkinson's disease, Alzheimer's disease, depression, epilepsy, autism, and rare disorders. We also describe several clinical drugs that target potassium or calcium channels in nerve cells and could be u

关键词： astrocytes calcium channels central nervous system extracellular ion concentration microglia neurological disorders neurons potassium channels

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Long-term follow-up of auditory performance and speech perception and effects of age on cochlear implantation in children with pre-lingual deafness

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Chinese Medical Journal 2019年第16期132卷 1925-1934页

作者： Jing Lyu Ying Kong Tian-Qiu Xu Rui-Juan Dong Bei-Er Qi Shuo Wang Yong-Xin Li Hai-Hong Liu xue-qing chen Department of Otolaryngology Head and Neck SurgeryBeijing Tongren HospitalCapital Medical UniversityBeijing Institute of OtolaryngologyBeijing 100005China Beijing Key Laboratory for Pediatric Diseases of Otolaryngology Head and Neck SurgeryDepartment of OtolaryngologyHead and Neck SurgeryBeijing Children's HospitalCapital Medical UniversityNational Center for Children's HealthBeijing 100045China.

Background: The development of auditory and speech perception ability of children with hearing loss is affected by many factors after they undergo cochlear implantation (CI). Age at CI (CI age) appears to play an important role among these factors. This study aimed to evaluate the development of auditory and speech perception ability and explore the impact of CI age on children with pre-lingual deafness present before 3 years of age. Methods: Two hundred and seventy-eight children with pre-lingual deafness (176 boys and 102 girls) were included in this study, and the CI age ranged from 6 to 36 months (mean age, 19 months). Categorical auditory performance (CAP) was assessed to evaluate auditory ability, and the speech intelligibility rating was used to evaluate speech intelligibility. The evaluations were performed before CI and 1, 3, 6, 12, 18, 24, 36, 48, and 60 months after CI. Results: The auditory ability of the pre-lingually hearing-impaired children showed the fastest development within 6 months after CI (k = 0.524, t = 30.992, P 0.05). The optimal cutoff age for CI was 15 months. Conclusions: Within 5 years after CI, the auditory and speech ability of young hearing-impaired children continuously improved, although speech development lagged behind that of hearing. An earlier CI age is recommended;the optimal cutoff age for CI is at 15 months.

关键词： Categorical auditory performance Speech intelligibility rating Cochlear implantation Children Pre-lingual deafness Age at cochlear implantation

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Recent progress of laser driven particle acceleration at Peking University

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Frontiers of physics 2013年第5期8卷 577-584页

作者： xue-qing Yan chen Lin Hai-Yang Lu Kun Zhu Yu-Bin Zou Hong-Yong Wang Bing Liu Shuan Zhao Jiao Zhu Yi-Xing Geng, He-Zheng Fu Yong Shang Chao Cao Yin-Ren Shou Wei Song Yuan-Rong Lu Zhong-Xi Yuan Zhi-Yu Guo Xian-Tu He Jia-Er chen State Key Laboratory of Nuclear Physics and Technology and CAPT Peking University Beijing 100871 China

Recently, radiation pressure acceleration （RPA） has been proposed and extensively studied, which shows that circularly polarized （CP） laser pulses can accelerate mono-energetic ion bunches in a phase-stable-acceleration （PSA） way from ultrathin foils. It is found that self-orgizing proton beam can be stably accelerated to GeV in the interaction of a CP laser with a planar target at 1022 W/cm2. A project called Compact LAser Plasma proton Accelerator （CLAPA） is approved by MOST in China recently. A prototype of laser driven proton accelerator （1 to 15 MeV/1 Hz） based on the PSA mechanism and plasma lens is going to be built at Peking University in the next five years. It will be upgraded to 200 MeV later for applications such as cancer therapy, plasma imaging and fast ignitiou for inertial confine fusion.

关键词： phase-stable acceleration (PSA) laser ion acceleration ignition

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Construction of 3D porous Cu_(1.81)S/nitrogen-doped carbon frameworks for ultrafast and long-cycle life sodium-ion storage

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International Journal of Minerals,Metallurgy and Materials 2025年第1期32卷 191-200页

作者： chen chen Hongyu xue Qilin Hu Mengfan Wang Pan Shang Ziyan Liu Tao Peng Deyang Zhang Yongsong Luo Key Laboratory of Microelectronics and Energy of Henan Province Henan Joint International Research Laboratory of New Energy Storage TechnologyCollege of Physics and Electronic EngineeringXinyang Normal UniversityXinyang 464000China School of Physics and Electronic Engineering Nanyang Normal UniversityNanyang 473061China

Transition metal sulfides have great potential as anode mterials for sodium-ion batteries(SIBs)due to their high theoretical specific ***,the inferior intrinsic conductivity and large volume variation during sodiation-desodiation processes seriously affect its high-rate and long-cyde performance,unbeneficial for the application as fast-charging and long-cycling SIBs ***,the three-dimensional porous Cu_(1.81)S/nitrogen-doped carbon frameworks(Cu_(1.81)S/NC)are synthesized by the simple and facile sol-gel and annealing processes,which can accommodate the volumetric expansion of Cu_(1.81)S nanoparticles and accelerate the transmission of ions and electrons during Na^(+)insertion/extraction processes,exhibiting the excellent rate capability(250.6 mA·g^(-1)at 20.0 A·g^(-1))and outstanding cycling stability(70% capacity retention for 6000 cycles at 10.0 A·g^(-1))for ***,the Na-ion full cells coupled with Na_(3)V_(2)(PO_(4))_(3)/C cathode also demonstrate the satisfactory reversible specific capacity of 330.5 mAh·g^(-1)at 5.0 A·g^(-1)and long-cycle performance with the 86.9% capacity retention at 2.0 A·g^(-1)after 750 *** work proposes a promising way for the conversionbased metal sulfides for the applications as fast-charging sodium-ion battery anode.

关键词： copper sulfide nanoparticles porous carbon framework fast charging long-cycle performance sodium-ion full batteries

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基于DWT-SVD和QR码的抗几何攻击数字水印技术

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包装工程 2016年第11期37卷 158-163页

作者：薛青晨吴丹陈大庆陈维霞顾济华苏州大学苏州215006

目的为了抵御QR码数字水印图像的几何攻击,提出一种基于奇异值分解和离散小波变换的数字水印技术。方法利用QR码编码规则,将文本信息编码生成宿主图像;将宿主图像进行对数极坐标变换,然后进行一级小波分解,利用奇异值分解原理在其低频... 详细信息

目的为了抵御QR码数字水印图像的几何攻击,提出一种基于奇异值分解和离散小波变换的数字水印技术。方法利用QR码编码规则,将文本信息编码生成宿主图像;将宿主图像进行对数极坐标变换,然后进行一级小波分解,利用奇异值分解原理在其低频分块中嵌入置乱后的水印图像,形成含水印的QR码图像。结果实验结果表明,该算法可以使QR码水印图像抵抗旋转、缩放、平移(RST)等几何攻击。结论算法有良好的鲁棒性和不可见性,能够作为一个可行的版权保护和认证的工具。

关键词：离散小波变换奇异值分解 QR码几何攻击

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