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Sustained release of Semaphorin 3A from a-tricalcium phosphate based cement composite contributes to osteoblastic differentiation of MC3T3-E1 cells

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Frontiers of Materials Science 2015年第3期9卷 282-292页

作者： Jin-Ning WANG Bin PI Peng WANG xue-Feng li hui-lin YANG xue-Song ZHU Department of Orthopaedic Surgery The first Affiliated Hospital of Soochow University Suzhou 215006 China

The reinforcement of calcium phosphate materials with silk fibroin （SF） has been one of the strategies to overcome the brittleness. However, the lack of osteoinductivity may still restrict their further use. This study aimed to investigate the biocompatibility and osteogenesis capacity of a novel Semaphorin 3A-loaded chitosan microspheres/SF/a-tricalcium phosphate composite （Sema3A CMs/SF/a-TCP） in vitro. Sema3A was first incorporated into CMs, and the Sema3A CMs/SF/a-TCP composite was then prepared. The morphology of the CMs was observed using SEM. The in vitro release kinetics, cytotoxicity, and cell compatibility were evaluated, and the real-time quantitative polymerase chain reaction （RT-qPCR） and activity of alkaline phosphatase （ALP） were used to evaluate the osteogenesis capacity of the composite. The in vitro release of Sema3A from the Sema3A CMs/SF/a-TCP composite showed a temporally controlled manner. The extract of the Sema3A CMs/SF/a-TCP composite presented no obvious side effect on the MC3T3-E1 cell proliferation, nor promote cell proliferation. The MC3T3-E1 cells were well-spread and presented an elongated shape on the Sema3A CMs/SF/a-TCP composite surface; the ALP activity and the osteogenic-related gene expression were higher than those seeded on the surface of the CMs/SF/a-TCP and SF/a-TCP composites. In conclusion, Sema3A CMslSF/a-TCP has excellent biocompatibility and contributes to the osteoblastic differentiation of MC3T3-E1 cells.

关键词： α-tricalcium phosphate (o-TCP) silk fibroin (SF) Semaphorin 3A osteo-blastic differentiation MC3T3-EI cell

Activity Enhancement of Pt/Ba/Al_(2)O_(3) Mixed with Mn/Ba/Al_(2)O_(3) for NO_(x) Storage-reduction by Hydrogen

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Chinese Chemical Letters 2006年第10期17卷 1357-1360页

作者： Jian Hua XIAO xue hui li Sha DENG Fu Rong WANG Le Fu WANG School of Chemical and Energy Engineering South China University of Technology Guangzhou 510640

Abstract： Mn/Ba/Al2O3 catalyst for NO oxidation-storage and Pt/Ba/Al2O3 catalyst mixed with Mn/Ba/Al2O3 for NOx storage-reduction by hydrogen were investigated. The results showed that Mn/Ba/Al2O3 had large nitrogen oxides storage capacity （397.9 μmolg^-1） under lean burn condition. When Pt/Ba/Al2O3 catalyst was mixed with Mn/Ba/Al2O3 in equal weight proportion, the NOx conversion increased between 250 ℃ and 500 ℃ under the dynamic lean-rich burn conditions, and the maximum NOx conversion increased from 95.4% to 98.2%. Mn/Ba/Al2O3 has promoted NOx storing in the lean stage and improved NOx reduction efficiency in the rich stage, these might result in higher NOx conversion over the low Pt loading content catalyst.

关键词： Nitrogen oxides storage-reduction lean burn Pt/Ba/Al2O3 Mn/Ba/Al2O3

Sol-Gel Synthesis of Normal Spinel liMn_2O_4 and Its Characteristics

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Journal of Wuhan University of Technology(Materials Science) 2002年第3期17卷 1-4页

作者： LEI Jia-heng SUN Yu-bin CHEN Yong-xi WANG Jin-rong xue li-hui Wuhan University of Technology

Normal spinel liMn 2O 4 was synthesized by sol-gel method using lithium nitrate,manganese nitrate,citric acid and ethylene glycol as raw materials. liMn 2O 4 was characterized by XRD,TG-DTA,IR,SEM and *** optimum conditions for the synthesis were *** acid and ethylene glycol were mixed with molar ratio of 0.25,and the mixture was esterified at 140℃ for 4 *** lithium nitrate and manganese nitrate were added with molar ratio of 0.6. In the system,the total molar of cations was equal to that of citric acid. At last, reflux the system at 105℃ for 2 hours. Dried gel was fired at 600℃ for 8 hours. Particle diameters of raw product were about 100 nm mainly. Further research shows that lithium ion of liMn 2O 4 is easy to be extracted,and normal spinel λ-MnO 2 can be obtained after lithium ion extraction.

关键词： sol gel method synthesis of liMn 2O 4 λ MnO 2

FeNiS_(2)/reduced graphene oxide electrocatalysis with reconstruction to generate FeNi oxo/hydroxide as a highly-efficient water oxidation electrocatalyst

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Rare Metals 2022年第12期41卷 4127-4137页

作者： Xiao-Zhen Ren xue-hui li Yu-Jie Peng Guan-Zhi Wang Jie Yin Xing-Chuan Zhao Wei Wang Xiao-Bai Wang School of Materials Science and Engineering Liaocheng UniversityLiaocheng 252000China School of Metallurgy and Chemistry Engineering Jiangxi University of Science and TechnologyGanzhou 341000China Department of Materials Application Research AVIC Manufacturing Technology InstituteBeijing 100024China

It is critical to developing electrocatalysts with highly active and cost-effective for oxygen evolution to resolve environmental pollution and energy issues,in which FeNi-based nanomaterials hold a great ***,(Fe_(0.33 )Ni_(0.67))S_(2) and(Fe_(0.33 )Ni_(0.67))S_(2)/reduced graphene oxide(rGO)-x%(x=10,20) composites,which exhibited highly efficient oxygen evolution reaction(OER)electrocatalytic activity under alkaline conditions,were synthesized via a hydrothermal approach and following thermal treatment with sulfur *** from the integrated structure of(Fe_(0.33 )Ni_(0.67))S_(2)and support of conductive graphene backbones,(Fe_(0.33 )Ni_(0.67))S_(2)/rGO-20%electrocatalyst showed the best OER activity with an overpotential of 172 mV at 10 mA·cm^(-2)and Tafel slopes of 45 mV·decade^(-1).The composition,phase,and surface structure of the catalyst were characterized before and after OER *** results indicated that crystal phase of the catalyst was reconstructed to the amorphous crystalline features after OER,with oxidation of iron-nickel sulfide and appearance of Ni-Fe oxo/hydroxide species,which may play a crucial role in the high OER performance as the ***,in a two-electrode system towards overall water splitting with(Fe_(0.33 )Ni_(0.67))S_(2)/rGO-20%/NF and Pt/C/NF as the anode and cathode,respectively,the catalysts exhibited excellent catalytic performance with the voltage of only 1.42 V at 10 mA·cm^(-2).

关键词： Ternary nickel-iron sulfide Reduced graphene oxide(rGO) Electrocatalysts Water oxidation Self-reconstruction

OSTEOCLASTIC RESORPTION OF CANCELLOUS BONE TISSUES OF FEMORAL NECK IN HIP FRACTURE PATIENTS (A SCANNING ELECTRON MICROSCOPIC STUDY)

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中华创伤杂志 1997年第2期 71-76页

作者： CHAI Ben fu, TANG xue ming, li hui Shanghai Institute of Traumatology and Orthopaedics Shanghai Second Medical University, Shanghai 200025

ＯＳＴＥＯＣＬＡＳＴＩＣＲＥＳＯＲＰＴＩＯＮＯＦＣＡＮＣＥＬＬＯＵＳＢＯＮＥＴＩＳＳＵＥＳＯＦＦＥＭＯＲＡＬＮＥＣＫＩＮＨＩＰＦＲＡＣＴＵＲＥＰＡＴＩＥＮＴＳ（ＡＳＣＡＮＮＩＮＧＥＬＥＣＴＲＯＮＭＩＣＲＯＳＣＯＰＩＣ... 详细信息

ＯＳＴＥＯＣＬＡＳＴＩＣＲＥＳＯＲＰＴＩＯＮＯＦＣＡＮＣＥＬＬＯＵＳＢＯＮＥＴＩＳＳＵＥＳＯＦＦＥＭＯＲＡＬＮＥＣＫＩＮＨＩＰＦＲＡＣＴＵＲＥＰＡＴＩＥＮＴＳ（ＡＳＣＡＮＮＩＮＧＥＬＥＣＴＲＯＮＭＩＣＲＯＳＣＯＰＩＣＳＴＵＤＹ）ＣＨＡＩＢｅｎ－ｆｕ（...

关键词： HIP FRACTURE NECK FEMORAL PATIENTS CANCELLOUS BONE

Purification and Identification of a Novel Protein Isolated from Panax quinquefolium and Evaluation of its In vitro Antioxidant Properties

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World Journal of Traditional Chinese Medicine 2019年第4期5卷 236-242页

作者： xue-hui li Yang Yang Zhao Yu Ning Xu Xiao-Lei Tang xue-Yuan Bai Si-Ming Wang Hao-Yuan Chen Da-Qing Zhao li liu Bin Qi Jilin Ginseng Academy Changchun University of Chinese Medicine College of Pharmacy Changchun University of Chinese MedicineChangchunChina

Objective:A novel protein was first purified from Panax quinquefolius L.(AGNP),and in vitro antioxidant activities of AGNP were first studied in this ***:AGNP was purified by Ion-exchange chromatography and Gel-filtration *** chemical characterizations of AGNP were tested by sodium dodecyl sulfate–polyacrylamide gel electrophoresis(SDS-PAGE),high-pressure gel-filtration chromatography,MALDI-TOF-MS and *** vitro antioxidant effects were tested in simple antioxidant assay including 2,2-diphenylpicrylhydrazyl radical scavenging,superoxide radical(O2^-)scavenging,hydroxyl radical(OH)scavenging,and ferric-reducing ***:The results showed which the content of AGNP measured by Bradford method was 2.42 mg/m L and the subunit molecular weight of AGNP measured by sodium dodecyl sulfate–polyacrylamide gel electrophoresis(SDS-PAGE)was 15 *** AGNP molecular weight was 15,114 Da both of SDS-PAGE and mass spectrum *** result of high-pressure gel-filtration chromatography demonstrated that the molecular weight of AGNP was 31,086 Da,which implied that AGNP was a *** in vitro Antioxidant results indicated that AGNP had obvious effects to remove the free radicals in ***:In conclusion,AGNP had more powerful antioxidant capacity and it can be used as an effective natural antioxidant to alleviate oxidative stress.

关键词： In vitro antioxidant properties novel protein Panax quinquefolium

Lattice vibrational characteristics,crystal structures and dielectric properties of non-stoichiometric Nd_((1+x))(Mg_(1/2)Sn_(1/2))O_(3) ceramics

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Journal of Materiomics 2020年第3期6卷 476-484页

作者： Zhikai Cao En-Cai Xiao xue-hui li Zhenxing Yue Guohua Chen Ying Chen Kaixin Song Huanfu Zhou Ze-Ming Qi Feng Shi School of Material Science&Engineering Shandong University of Science and TechnologyQingdao266590China College of Arts and Design Shandong Jiaotong UniversityJinan250357China State Key Laboratory of New Ceramics and Fine Processing School of Materials Science and EngineeringTsinghua UniversityBeijing100084China Guangxi Key Laboratory of Information Materials Guilin University of Electronic TechnologyGuilin541004China Key Laboratory of Inorganic Functional Materials and Devices Shanghai Institute of CeramicsChinese Academy of Sciences1295 Dingxi RoadShanghai200050China School of Electronics Information Hangzhou Dianzi UniversityHangzhou310018China Guangxi Ministry-Province Jointly-Constructed Cultivation Base for State Key Laboratory of Processing for Non-ferrous Metal and Featured Materials School of Materials Science and EngineeringGuilin University of TechnologyGuilin541004China National Synchrotron Radiation Laboratory University of Science and Technology of ChinaHefei230029China Key Laboratory of Processing and Testing Technology of Glass&Functional Ceramics of Shandong Province School of Materials Science and EngineeringQilu University of Technology(Shandong Academy of Sciences)Jinan250353China

Non-stoichiometric Nd_((1+x))(Mg_(1/2)Sn_(1/2))O_(3)(-0.04=x≤0.04,NMS)ceramics were fabricated via a conventional solid-state reaction *** structures and morphologies were investigated by Xray diffraction(XRD)and scanning electron microscopy(SEM),*** main crystalline phase is monoclinic Nd(Mg_(1/2)Sn_(1/2))O_(3) with a double perovskite structure(P21/n space group)for the NMS system proved by *** sample at x=0.01 has the best crystallinity and evenly distributed crystal grains observed by *** optimum performances(ε_(r)=19.87,Q×f=41840 GHz,f=12.05 GHz)are obtained at x=*** vibrational modes of the Raman spectra were assigned and illustrated,in *** dielectric properties obtained by fitting infrared reflectance spectra with the help of four-parameter semi-quantum model are consistent with the calculated values by microscopic polarization and damping *** reverse translational vibration of the NdeMgO_(6),the F_(5u)^((5)) mode,provides the greatest contribution to the dielectric *** relationships between crystal structures and dielectric properties were mainly established using lattice vibrational modes as a media.

关键词： Microwave dielectric ceramics Non-stoichiometric Lattice vibrational characteristics Crystal structures Dielectric properties

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浅析电瓶车火灾防范对策

消防科学与技术 2017年第8期36卷 1174-1176页

作者：薛钧李辉台州市消防支队浙江台州318020 杭州市消防支队浙江杭州310000

以一起电动自行车亡人火灾为例,通过调查访问、现场勘验,确定起火原因,从电动自行车的停放位置、火灾发生的时间、电瓶车的材质、人员的充电习惯、电瓶车的设计标准等方面分析灾害成因,并进一步提出电动自行车火灾的防范对策,包括提高... 详细信息

以一起电动自行车亡人火灾为例,通过调查访问、现场勘验,确定起火原因,从电动自行车的停放位置、火灾发生的时间、电瓶车的材质、人员的充电习惯、电瓶车的设计标准等方面分析灾害成因,并进一步提出电动自行车火灾的防范对策,包括提高电动自行车生产的行业技术标准、加强流通领域的行业监管、明确停放区的消防要求、开展相关的消防宣传教育等。

关键词：电动自行车火灾调查火灾原因消防监督

Decoding the nexus:branched-chain amino acids and their connection with sleep,circadian rhythms,and cardiometabolic health

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Neural Regeneration Research 2025年第5期20卷 1350-1363页

作者： hui li Laurent Seugnet Department of Neurology Xijing HospitalXi’anShaanxi ProvinceChina Centre de Recherche en Neurosciences de Lyon Integrated Physiology of the Brain Arousal Systems(WAKING)UniversitéClaude Bernard Lyon 1INSERM U1028CNRS UMR 5292BronFrance

The sleep-wake cycle stands as an integrative process essential for sustaining optimal brain function and,either directly or indirectly,overall body health,encompassing metabolic and cardiovascular *** the heightened metabolic activity of the brain,there exists a considerable demand for nutrients in comparison to other *** these,the branched-chain amino acids,comprising leucine,isoleucine,and valine,display distinctive significance,from their contribution to protein structure to their involvement in overall metabolism,especially in cerebral *** the first amino acids that are released into circulation post-food intake,branched-chain amino acids assume a pivotal role in the regulation of protein synthesis,modulating insulin secretion and the amino acid sensing pathway of target of ***-chain amino acids are key players in influencing the brain's uptake of monoamine precursors,competing for a shared *** their involvement in protein synthesis,these amino acids contribute to the metabolic cycles ofγ-aminobutyric acid and glutamate,as well as energy ***,they impact GABAergic neurons and the excitation/inhibition *** rhythmicity of branchedchain amino acids in plasma concentrations,observed over a 24-hour cycle and conserved in rodent models,is under circadian clock *** mechanisms underlying those rhythms and the physiological consequences of their disruption are not fully *** sleep,obesity,diabetes,and cardiovascular diseases can elevate branched-chain amino acid concentrations or modify their oscillatory *** mechanisms driving these effects are currently the focal point of ongoing research efforts,since normalizing branched-chain amino acid levels has the ability to alleviate the severity of these *** this context,the Drosophila model,though underutilized,holds promise in shedding new light on these *** findings indicate its po

关键词： branched-chain amino acids cardiovascular health circadian clock drosophila insulin metabolism sleep γ-aminobutyric acid