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The mechanism by which hyperbaric oxygen treatment alleviates spinal cord injury: genome-wide transcriptome analysis

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Neural Regeneration Research 2022年第12期17卷 2737-2742页

作者： Zhen-Cheng Sun Fang Liang Jing Yang Yong Hai Qing-Jun Su xue-hua liu Department of Orthopedic Surgery Beijing Chaoyang HospitalCapital Medical UniversityBeijingChina Department of Hyperbaric Oxygen Beijing Chaoyang HospitalCapital Medical UniversityBeijingChina

Accumulating studies have demonstrated that hyperbaric oxygen(HBO)treatment alleviates spinal cord injury(SCI).However,the underlying mechanism by which HBO alleviates SCI remains to be *** this study,we performed genome-wide transcriptional profiling of the spinal cord between SCI mice and mice that received HBO treatment by high-throughput RNA sequencing at 1 week after *** also compared genome-wide transcriptional profiles from SCI mice and sham-operated *** found 76 differentially co-expressed genes in sham-operated mice,SCI mice,and HBO-treated SCI *** Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis,we identified the biological characteristics of these differentially expressed genes from the perspectives of cell component,biological process,and molecular *** also found enriched functional pathways including ferroptosis,calcium signaling pathway,serotonergic synapse,hypoxia-inducible factor-1 signaling pathway,cholinergic synapse,and neuroactive ligand-receptor *** performed quantitative reverse transcription-polymerase chain reaction and validated that HBO treatment decreased the expression of Hspb1(heat shock protein beta 1),Hmox1(heme oxygenase 1),Ftl1(ferritin light polypeptide 1),Tnc(tenascin C)and Igfbp3(insulin-like growth factor binding protein 3)and increased the expression of Slc5a7(solute carrier family 5 choline transporter member 7)after *** results revealed the genome-wide transcriptional profile of the injured spinal cord after HBO *** findings contribute to a better understanding of the mechanism by which HBO treats SCI and may provide new targets for SCI intervention.

关键词： Ftl1 genome-wide transcriptome Hmox1 Hspb1 hyperbaric oxygen Igfbp3 Slc5a7 spinal cord injury Tnc

Primary squamous cell carcinoma with sarcomatoid differentiation of the kidney associated with ureteral stone obstruction: A case report

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World Journal of Clinical Cases 2022年第32期10卷 11942-11948页

作者： xue-hua liu Qian-Ming Zou Jia-Dong Cao Zhi-Chao Wang Department of Urology Surgery Fangcun Hospital of Guangdong Provincial Hospital of traditional Chinese MedicineGuangzhou 510120Guangdong ProvinceChina

BACKGROUND Primary squamous cell carcinoma(SCC)with sarcomatoid differentiation of the kidney was rarely *** disease is usually related to renal stones,and due to a lack of symptoms and radiological features,patients usually attend the hospital with late stage *** SUMMARY A 54-years-old female presented with left flank pain and an abdominal mass for 6 *** studies revealed that the left kidney was enlarged and massive hydronephrosis was present.A stone was seen in the ureteropelvic *** patient subsequently underwent left radical nephrectomy,and histopathological examination of the mass revealed a poorly differentiated renal SCC with sarcomatoid *** primary surgery,the patient received four cycles of *** months later,the patient developed adrenal,lymph,and uterine appendage *** SCC of the kidney has a poor prognosis,and should be considered in patients with a renal mass,long-standing urinary calculi and massive hydronephrosis.

关键词： Squamous cell carcinoma Sarcomatoid differentiation Kidney Ureteral stone Case report

Rapid quantification of multi-components in alcohol precipitation liquid of Codonopsis Radix using near infrared spectroscopy(NIRS)

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2017年第5期18卷 383-392页

作者： Yu LUO Wen-long LI Wen-hua huaNG xue-hua liu Yan-gang SONG Hai-bin QU Pharmaceutical informatics Institute Zhejiang UniversityHangzhou 310058China Livzon(Group)Limin Pharmaceutical Factory Shaoguan 512028China

A near infrared spectroscopy（NIRS） approach was established for quality control of the alcohol precipitation liquid in the manufacture of Codonopsis Radix. By applying NIRS with multivariate analysis, it was possible to build variation into the calibration sample set, and the Plackett-Burman design, Box-Behnken design, and a concentrating-diluting method were used to obtain the sample set covered with sufficient fluctuation of process parameters and extended concentration information. NIR data were calibrated to predict the four quality indicators using partial least squares regression（PLSR）. In the four calibration models, the root mean squares errors of prediction（RMSEPs） were 1.22 μg/ml, 10.5 μg/ml, 1.43 μg/ml, and 0.433% for lobetyolin, total flavonoids, pigments, and total solid contents, respectively. The results indicated that multi-components quantification of the alcohol precipitation liquid of Codonopsis Radix could be achieved with an NIRS-based method, which offers a useful tool for real-time release testing（RTRT） of intermediates in the manufacture of Codonopsis Radix.

关键词： Near infrared spectroscopy Codonopsis Radix Alcohol precipitation Real-time release testing Multicomponents quantification

Pancreatic and peri-pancreatic lesions mimic pancreatic islet cell tumor in multidetector computed tomography

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Chinese Medical Journal 2011年第11期124卷 1720-1725页

作者： xue hua-dan liu Wei XIAO Yu SUN Hao WANG Xuan LEI Jing JIN Zheng-yu Departmentor RadiologyPeking Union Medical College Hospital Beijing 100730 China

Objective This pictorial review aimed to summarize the most possible differential diagnosis of pancreatic islet cell tumor （PICT）. Data sources Data used in this review were mainly from Medline and Pubmed in English. And all clinical images in this review were from Department of Radiology, Peking Union Medical College Hospital, Beijing, China. Study selection Cases of pancreatic cystadenoma, solid pseudo-papillary tumor of the pancreas, pancreatic metastasis, pancreatic adenocarcinoma, para-pancreatic neuroendocrine tumors, Castleman disease, gastrointestinal stromal tumor, splenic artery aneurysm and accessory spleen were selected in this pictorial review for differential diagnosis of PICT.

关键词： computed tomography islet cell tumor differential diagnosis pancreas mimic

Effects of Lanerkang-A on Immune Efficacy of PRRS Vaccines and Growth Performance Indicators of Piglets

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Animal Husbandry and Feed Science 2010年第4期2卷 32-33,35页

作者： LI xue-hua liu Jun-wei GUO Yan-feng CUI Jin-zhong College of Animal Science Henan Institute of Science and Technology Xinxiang 453003 China

[ Objective] To study the effects of Lanerkang-A on the immunity and growth performance of piglets and lay a base for the development of green medicine on immunity stimulator, [ Method] A total of 50 healthy piglets were randomly divided into two groups, the control group （Group 1 ） and the test group （Group 2）, 25 piglets in each group. All the piglets were intramuscularly injected with 2 ml porcine reproductive and respiratory syndrome （PRRS） propolis inactivated vaccine at 25 days old. The piglets in the Group 2 were fed with feed containing Lanerkang-A （ 10 ml/kg） at 42 days old. After 7-d feeding, the antibody titer against PRRSV and the cytoimmunity were detected using ELISA and E-rosette, respectively. [ Result] Lanerkang-A increased antibody titer against PRRSV by 4 titers and increased E-rosette forming rate by 7%. The average daily weight gain increased by 15.68%. [ Conclusion] Lanerkang-A can greatly enhance the immunological function of body, increase the daily gain of piglets, as well as improve their growth performance.

关键词： Piglets PRRS vaccines Immune efficacy Growth performance

Hyperbaric oxygen improves functional recovery of rats after spinal cord injury via activating stromal cell-derived factor-1/CXC chemokine receptor 4 axis and promoting brain-derived neurothrophic factor expression

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Chinese Medical Journal 2019年第6期132卷 699-706页

作者： Xiang-Long Meng Yong Hai Xi-Nuo Zhang Yun-Sheng Wang xue-hua liu Lin-Lin Ma Rong Yue Gang Xu Zhuo Li Orthopedic Department Beijing Chaoyang Hospital Capital Medical University Beijing 100020 China Hyperbaric Oxygen Department Beijing Chaoyang Hospital Capital Medical University Beijing 100020 China Hyperbaric Oxygen Department Xinjiang Uygur Autonomous Region People's Hospital Urumqi Xinjiang 830001 China

Background: Spinal cord injury (SCI) is a worldwide medical concern. This study aimed to elucidate the mechanism underlying protective effect of hyperbaric oxygen (HBO) against SCI-induced neurologic defects in rats via exploring the stromal cell-derived factor-1 (SDF-1)/CXC chemokine receptor 4 (CXCR4) axis and expression of brain-derived neurotrophic factor (BDNF). Methods: An acute SCI rat model was established in Sprague-Dawley rats using the Allen method. Sixty rats were divided into four groups (w = 15 in each group): sham-operated, SCI, SCI treated with HBO (SCI + HBO), and SCI treated with both HBO and AMD3100 (an antagonist of CXCR4;SCI + HBO + AMD) groups. The rats were treated with HBO twice a day for 3 days and thereafter once a day after the surgery for up to 28 days. Following the surgery, neurologic assessments were performed with the Basso-Bettie-Bresnahan (BBB) scoring system on postoperative day (POD) 7, 14, 21, and 28. Spinal cord tissues were harvested to assess the expression of SDF-1, CXCR4, and BDNF at mRNA and protein levels, using quantitative real-time polymerase chain reaction, Western blot analysis, and histopathologic analysis. Results: HBO treatment recovered SCI-induced descent of BBB scores on POD 14,(1.25±0.75 vs. 1.03 ±0.66, P< 0.05), 21 (5.27± 0.89 vs. 2.56± 1.24, P< 0.05), and 28 (11.35±0.56 vs. 4.23± 1.20, P<0.05) compared with the SCI group. Significant differences were found in the mRNA levels of SDF-1 (mRNA: day 21, SCI + HBO vs. SCI + HBO + AMD, 2.89± 1.60 vs. 1.56±0.98, P<0.05), CXCR4 (mRNA: day 7, SCI + HBO vs. SCI, 2.99± 1.60 vs. 1.31 ±0.98, P<0.05;day 14, SCI + HBO vs. SCI + HBO + AMD, 4.18± 1.60 vs. 0.80±0.34, P<0.05;day 21, SCI + HBO vs. SCI, 2.10±1.01 vs.1.15±0.03, P<0.05), and BDNF (mRNA: day 7, SCI + HBO vs. SCI, 3.04±0.41 vs. 2.75±0.31, P<0.05;day 14, SCI + HBO vs. SCI, 3.88± 1.59 vs. 1.11 ±0.40, P<0.05), indicating the involvement of SDF-1/CXCR4 axis in the protective effect of HBO. Conclusions: HBO might promote t

关键词： Brain-derived neurotrophic factor CXC chemokine receptor 4 Hyperbaric oxygen Neurotrophic Stromal cell-derived factor-1 Spinal cord injury

Influence of introducing Zr,Ti,Nb and Ce elements on externally solidified crystals and mechanical properties of high-pressure die-casting Al–Si alloy

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International Journal of Minerals,Metallurgy and Materials 2025年第1期32卷 147-153页

作者： Junjie Li Wenbo Yu Zhenyu Sun Weichen Zheng Liangwei Zhang Yanling xue Wenning liu Shoumei Xiong Center of Materials Science and Engineering School of Mechanical and Electronic Control EngineeringBeijing Jiaotong UniversityBeijing 100044China Traffic&Transportation Engineering Central South UniversityChangsha 410075China Shanghai Synchrotron Radiation Facility Shanghai Advanced Research InstituteChinese Academy of SciencesShanghai 201204China School of Materials Science and Engineering Tsinghua UniversityBeijing 100084China

High pressure die casting(HPDC)AlSi10Mn Mg alloy castings are widely used in the automobile *** can optimize the mechanical properties of castings through heat treatment,while the release of thermal stress arouses the deformation of large integrated ***,the development of non-heat treatment Al alloys is becoming the hot *** addition,HPDC contains externally solidified crystals(ESCs),which are detrimental to the mechanical properties of *** achieve high strength and toughness of non-heat treatment die-casting Al-Si alloy,we used AlSi9Mn alloy as matrix with the introduction of Zr,Ti,Nb,and *** influences on ESCs and mechanical properties were systematically investigated through three-dimensional reconstruction and thermodynamic *** results reveal that the addition of Ti increased ESCs'size and porosity,while the introduction of Nb refined ESCs and decreased ***,large-sized Al_3(Zr,Ti)phases formed and degraded the mechanical *** introduction of Ce resulted in the poisoning effect and reduced mechanical properties.

关键词： aluminium alloy high-pressure die-casting externally solidified crystals non-heat treatment

Polo-like Kinase1 Gene Expression and its Clinicopathological Significance in gastric Carcinoma

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上海交通大学学报（医学版） 2006年第5期26卷 510-510页

作者： CHEN xue-hua LAN Bin liu Bing-ya QU Ying ZHANG Xiao-qing CAI Qu ZHU Zheng-gang Department of Surgery Shanghai Institute of Digestive Surgery Ruijin Hospital School of Medicine Shanghai Jiaotong University Shanghai 200025 China

Objective To clarify the polo-like kinase1 (PLK1) expression in human gastric cancer tissue and its clinicopathological sig-nificance in gastric carcinoma. Methods PLK1 expression in 60 cancer tissues and their corresponding noncancerous tissues from gas-tric cancer patients was measured by both real-time quantitative RT-PCR and western blot assay. Immunohistochemistry was used to detectPLK1 protein expression in eighty-nine paraffin-embedded samples. Results The PLK1 mRNA and protein expression level in the 60fresh cancer tissues was significantly higher than that in noncancerous tissues (P<0.0001,P=0.031 respectively). In paraffin-embed-ded samples, apart from its increased expression level, PLK1 was found to be in both cytoplasm and nucleus, double-site location onlyoccurred in poor-differentiated cancer, PLK1 expression intensity was associated with tumor differentiation (P=0.03), invasion(P=0.032), TNM stage (P=0.019), ki67 expression (P=0.011). The patients with negative PLK1 expression had better survivalrate than that with positive PLK1 expression (P=0.0292). Conclusion PLK1 may have clinicopathological value in tumor diagnosis,and may become another new bio-marker and therapeutic target for gastric cancer.

关键词：基因表达临床病理学胃癌定量设计

基于专家问卷的艾滋病皮肤瘙痒性病变中医诊疗规程研究

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中国中西医结合杂志 2013年第7期33卷 890-895页

作者：潘菊华王阶黄世敬薛柳华吴巍李兴旺王健中国中医科学院广安门医院中药研发中心北京100053 首都医科大学附属北京地坛医院感染科北京100015 中国中医科学院中医药防治艾滋病研究中心北京100700

目的建立艾滋病皮肤瘙痒性病变中医诊疗规程草案。方法通过文献调研和专家论证,建立艾滋病皮肤瘙痒性病变中医诊疗规程草案。采用两轮问卷调查中医或中西医结合艾滋病领域专家对诊断、治疗、调护等关键问题的接受程度及建议,并修订诊疗... 详细信息

目的建立艾滋病皮肤瘙痒性病变中医诊疗规程草案。方法通过文献调研和专家论证,建立艾滋病皮肤瘙痒性病变中医诊疗规程草案。采用两轮问卷调查中医或中西医结合艾滋病领域专家对诊断、治疗、调护等关键问题的接受程度及建议,并修订诊疗规程。结果第1轮完整问卷回收率为96%,专家对病史及体检、3个证型的辨证标准、血虚风燥的辨证用药和调护等条目的认可程度较高,对概述、辅助检查、血热生风和风淫湿胜的辨证用药等条目的变异系数(CV)在0.1603～0.2473之间,认识差异较大。根据回收意见,对规程进行修改,并开展第2轮问卷调查。第2轮完整问卷回收率为100%,专家对病史及体检、诊断标准、血虚风燥、风淫湿胜的辨证标准和血虚风燥的辨证用药等条目的CV为0,认可程度高,一致性好。其他条目的CV为0.0638～0.1439,所以差异程度比第1轮小,专家意见的一致性有较大提高。两轮调查中单个条目对总体评价结果所做出的贡献相差不大。通过两轮调查得到了该规程的修订版本。结论两轮问卷调查的专家积极性较好,集中程度和协调程度均较好。通过两轮问卷调查,对该规程的诊断、治疗、调护等关键问题基本达成了较好的共识。

关键词：瘙痒艾滋病人类免疫缺陷病毒中医诊疗规程问卷调查