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Mediating the Migration of Mesenchymal Stem Cells by Dynamically Changing the Density of Cell-selective Peptides Immobilized on β-Cyclodextrin-modified Cell-resisting Polymer Brushes

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Chinese Journal of Polymer Science 2020年第9期38卷 126-136,I0005页

作者： wang Du De-Teng Zhang xue-mei wang Tan-Chen Ren Chang-You Gao Ministry of Education Key Laboratory of Macromolecular Synthesis and Functionalization Department of Polymer Science and Engineering Zhejiang University

Dynamic control of mesenchymal stem cell(MSC)behaviors on biomaterial surface is critically involved in regulating the cell fate and tissue ***,a stimuli-responsive surface based on host-guest interaction with cell selectivity was developed to regulate migration of MSCs in situ by dynamic display of cell-specific ***-grafted MSC-affinitive peptides(EPLQLKM,Azo-E7)were grafted toβ-cyclodextran(β-CD)-modified poly(2-hydroxyethyl methacrylate)-b-poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate)(PHG)brushes,which were prepared by using surface-initiated atom transfer radical polymerization(SI-ATRP).X-ray photoelectron spectroscopy(XPS),quartz crystal microbalance(QCM),and water contact angle were used to characterize their structure and *** adhesion assay showed that the combination effect of resisting property of PHG and MSC-affinity of E7 could promote the selective adhesion of MSCs over other types of cells such as RAW264.7 macrophages and NIH3 T3 fibroblasts to some ***-Vis spectroscopy proved that the competing guest molecules,amantadine hydrochloride(Ama),could release Azo-E7 peptides from the CD surface to different extents,and the effect was enhanced when UV irradiation was employed *** a result,the decrease of cell adhesion density and migration rate could be achieved in *** cell density and migration rate could be reduced by over 40%by adding 20μmol/L Ama,suggesting that this type of surface is a new platform for dynamic regulation of stem cell behaviors in situ.

关键词： Host-guest interaction MSC-affinitive peptide Dynamic surface Cell adhesion Cell migration

Anemia status of infants and young children aged six to thirty-six months in Ma'anshan City:A retrospective study

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World Journal of Clinical Cases 2023年第28期11卷 6744-6753页

作者： xue-mei wang Qiong-Yao wang Jie Huang Department of Pediatric Ma’anshan Maternal and Child Health CenterMa’anshan 243011Anhui ProvinceChina Department of Hematology and Oncology Children's Hospital of Nanjing Medical UniversityNanjing 210000Jiangsu ProvinceChina

BACKGROUND Anemia in infants and young children can have long-term effects on cognitive and physical *** Ma'anshan City,China,there has been growing concern about the prevalence of anemia among children aged 6 to 36 *** the factors influencing this condition is crucial for targeted interventions and improving overall child health in the *** To analyze the anemia status and influencing factors of infants and young children aged 6 to 36 mo in Ma'anshan City,*** scientific evidence for reducing the incidence of anemia and improving the health level of children in this age *** The study encompassed 37698 infants and young children,aged from 6 to 36 mo,who underwent health examinations at the Ma'anshan Maternal and Child Health Hospital from January 2018 to October 2022 were included in the *** information,physical examination,and hemoglobin detection data were *** analysis was used to analyze the prevalence of anemia in children in the region,and univariate analysis was used to analyze the influencing factors of *** The mean hemoglobin level of infants and young children aged 9 to 36 mo increased with age,and the anemia detection rate decreased with *** anemia detection rate in rural infants aged 6,9,and 12 mo was higher than that in urban *** the anemia detection rate was higher in 6-mo-old boys than girls,it was higher in 24-mo-old girls than *** were statistically significant differences in the anemia detection rates among 9-mo-old and 12-mo-old infants with different nutritional statuses(emaciation,overweight,obese,and normal).Moreover,there were no statistically significant differences in anemia detection rates among infants and young children with different nutritional statuses at other ***,the anemia detection rates in obese infants aged 9 and 12 mo were higher than those in normal and overweight infants,with statistically significant differe

关键词： Infants Children Anemia Hemoglobin Anemia detection rates

Impact of Multidisciplinary Collaborative Intervention on Isolation Implementation in Prevention and Control of Multi-drug Resistance Infection

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Current Medical Science 2023年第1期43卷 198-205页

作者： Ying wang Xin-ping ZHANG Xiao-quan LAI xue-mei wang Naning Drum Tower Hospital Affiated to Nanjing University Medical School Nanjing210008China School of Medicine and Health Management Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan430030China Department of Nosocomial Infection Management Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430030China

Objective Contact precautions,especially the initiation of isolation,are important measures to prevent and control multidrug-resistant organisms(MDROs).However,the implementation in clinical practice remains *** study aimed to analyze the impact of multidisciplinary collaborative intervention on isolation implementation in multidrug-resistant infection,and determine the factors that affect the implementation of isolation *** A multidisciplinary collaborative intervention related to isolation was conducted at a teaching tertiary hospital in central China on November 1,*** information of 1338 patients with MDRO infection and colonization at 10 months before and after the intervention was ***,the issuance of isolation orders was retrospectively *** analysis and multivariate logistic regression analysis were performed to analyze the factors that affected the isolation *** The overall issuance rate of isolation orders was 61.21%,which increased from 33.12%to 75.88%(P<0.001)after the implementation of the multidisciplinary collaborative *** intervention(P<0.001,OR=0.166)was a promoting factor for the issuance of isolation orders,in addition to the length of stay(P=0.004,OR=0.991),department(P=0.004),and microorganism(P=0.038).Conclusion The isolation implementation remains far lower than policy *** collaborative interventions can effectively improve the compliance to isolation measures implemented by doctors,thereby promoting the standardized management of MDROs,and providing reference for further improving the quality of hospital infection management.

关键词： multidisciplinary collaborative intervention issuance of isolation orders multidrug-resistant organism infection and colonization isolation implementation rate

A Study of the Probe Effect on the Apparent Image of Biological Atomic Force Microscopy

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Chinese Chemical Letters 2001年第12期12卷 1113-1114页

作者： xue mei wang Lei QIN Long BA Zu Hong LU The State Key Lab of Coordination Chemistry Nanjing UniversityNanjing 210093 National Lab of Molecular and Biomolecular Electronics Southeast UniversityNanjing 210096

The probe effect on the apparent image of biological atomic force microscopy was explored in this study, and the potential of AFM in conformational study of gene related biological processes was illustrated by the spe... 详细信息

关键词： atomic force microscopy (AFM) absorption DNA recognition

1-Pyrenecarboxaldehyde thiosemicarbazone:A novel fluorescent molecular sensor towards mercury(Ⅱ) ion

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Chinese Chemical Letters 2010年第9期21卷 1124-1128页

作者： xue mei wang Hua Yan Xin Lu Feng Yong Chen Department of Chemistry and Material Engineering Logistic Engineering University Chongqing 401311 China Department of Applied Oil Engineering Logistic Engineering University Chongqing 401311 China Department of Barracks Management & Environmental Engineering Logistic Engineering University Chongqing 401311 China

A novel and simple fluorescent molecular sensor,1-pyrenecarboxaldehyde thiosemicarbazone（Hpytsc）,was *** higher sensitivity and selectivity to mercury（Ⅱ） ion were studied through absorption and emission *** UV-vis spectra show that the increasing mercury（Ⅱ） ion concentrations result in the decreasing absorption *** fluorescence monomer emission of Hpytsc is enhanced upon binding mercury（Ⅱ） ion,which should be due to the 1：1 complex formation between Hpytsc and metal ion.

关键词： Fluorescence sensor Mercury(Ⅱ) ion Pyrene derivatives Thiosemicarbazone

Artifacts in two-dimensional shear wave elastography of liver

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World Journal of Gastroenterology 2023年第21期29卷 3318-3327页

作者： Hui-Peng wang Peng-Chao Zheng xue-mei wang Liang Sang Department of Ultrasound The First Hospital of China Medical UniversityShenyang 110001Liaoning ProvinceChina

BACKGROUND Artifacts are common when using two-dimensional shear wave elastography(2-D SWE)to measure liver stiffness(LS),but they are poorly *** To investigate the presence and influence of artifacts in 2-D SWE of *** We included 158 patients with chronic liver disease,who underwent 2-D SWE examination by a novice and an expert.A cross line at the center of the elastogram was drawn and was divided it into four locations:top-left,top-right,bottom-left,and *** occurrence frequency of artifacts in different locations was *** influence of artifacts on the LS measurements was evaluated by comparing the elastogram with the most artifacts(EMA)and the elastogram with the least artifacts(ELA).RESULTS The percentage of elastograms with artifacts in the novice(51.7%)was significantly higher than that of the expert(19.6%)(P<0.001).It was found that both operators had the highest frequency of artifacts at bottom-left,followed by top-left and bottom-right,and top-right had the lowest *** LS values(LSVs)and standard deviation values of EMAs were significantly higher than those of ELAs for both *** intraclass correlation coefficient value of 0.96 was found in the LSVs of EMAs of the two operators,and it increased to 0.98 when the LSVs of the ELAs were *** operators had lower stability index values for EMAs than ELAs,but the difference was only statistically significant for the *** Artifacts are common when using 2-D SWE to measure LS,especially for the *** may lead to the overestimation of LS and reduce the repeatability and reliability of LS measurements.

关键词： Ultrasound Elastography Artifact Liver

Electrochemical study of the effect of functionalized nickel nanoparticles on cellular uptake of leukemia cancer cells in vitro

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Chinese Chemical Letters 2008年第5期19卷 577-580页

作者： Da Dong Guo Chun Hui Wu xue mei wang Bao An Chen State Key Lab of Bioelectronics （Chien-Shiung Wu Lab） Southeast University Nanjing 210096 China Zhongda Hospital School of Clinical Medical Southeast University Nanjing 210096 China

In this study, we have fabricated the functionalized nickel nanoparticles and investigated their effects on cellular uptake of quercetin in leukemia K562 cancer cells by using electrochemical assay. The results indicate that nickel nanoparticles could efficiently enhance the quercetin uptake and increase the intracellular accumulation in cancer cells, implying the great potential of functionalized nickel nanoparticles in target cancer therapy.

关键词： Nickel nanoparticles Leukemia cancer cells Quercetin Electrochemical assay

Bioinformatics analysis of aberrantly methylated-differentially expressed genes and pathways in hepatocellular carcinoma

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World Journal of Gastroenterology 2018年第24期24卷 2605-2616页

作者： Liang Sang xue-mei wang Dong-Yang Xu Wen-jing Zhao Department of Ultrasound The First Hospital of China Medical University

AIM To discover methylated-differentially expressed genes(MDEGs) in hepatocellular carcinoma(HCC) and to explore relevant hub genes and potential pathways. METHODS The data of expression profiling GSE25097 and methylation profiling GSE57956 were gained from GEO Datasets. We analyzed the differentially methylated genes and differentially expressed genes online using GEO2 R. Functional and enrichment analyses of MDEGs were conducted using the DAVID database. A protein-protein interaction(PPI) network was performed by STRING and then visualized in Cytoscape. Hub genes were ranked by cytoH ubba, and a module analysis of the PPI network was conducted by MCODE in Cytoscape software. RESULTS In total, we categorized 266 genes as hypermethylated, lowly expressed genes(Hyper-LGs) referring to endogenous and hormone stimulus, cell surface receptor linked signal transduction and behavior. In addition, 161 genes were labelled as hypomethylated, highly expressed genes(Hypo-HGs) referring to DNA replication and metabolic process, cell cycle and division. Pathway analysis illustrated that Hyper-LGs were enriched in cancer, Wnt, and chemokine signalling pathways, while Hypo-HGs were related to cell cycle and steroid hormone biosynthesis pathways. Based on PPI networks, PTGS2, PIK3 CD, CXCL1, ESR1, and MMP2 were identified as hub genes for Hyper-LGs, and CDC45, DTL, AURKB, CDKN3, MCM2, and MCM10 were hub genes for Hypo-HGs by combining six ranked methods of cytoH ubba. CONCLUSION In the study, we disclose numerous novel genetic and epigenetic regulations and offer a vital molecular groundwork to understand the pathogenesis of HCC. Hub genes, including PTGS2, PIK3 CD, CXCL1, ESR1, MMP2, CDC45, DTL, AURKB, CDKN3, MCM2, and MCM10, can be used as biomarkers based on aberrant methylation for the accurate diagnosis and treatment of HCC.

关键词： Hepatocellular carcinoma Methylation Gene expression Bioinformatics analysis

Ultrasonic elastography in clinical quantitative assessment of fatty liver

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World Journal of Gastroenterology 2010年第37期16卷 4733-4737页

作者： Yin-Yan Li xue-mei wang Yi-Xia Zhang Guo-Cheng Ou Department of Ultrasonic Diagnosis the First Af��liated Hospital of China Medical UniversityShenyang 110001Liaoning ProvinceChina

AIM: To investigate the clinical application of ultrasonic elastography in quantitative assessment of fatty liver grading. METHODS: A total of 105 patients with fatty liver were divided into mild group (n = 46), moderate group (n = 39), and severe group (n = 20). Forty-five healthy individuals served as a normal control group. All patients who underwent routine ultrasound scan and further ultrasonic elastography were evaluated accordingly to the evaluation standards for ultrasonic elastography. The ratio of surface areas of blue region/total surface area in the desired region was measured. RESULTS: Ultrasonic elastography technique, in comparison to traditional ultrasound, had a rather high consistence in grading of fatty liver [κ value = (95.3%63.6%)/(1%-63.6%) = 0.87, P = 0.001]. The score of ultrasonic elastography increased with the severity of fatty liver with a sensitivity of 97.14% and a specificity of 91.11%. A significant difference was found in the ratio of surface areas of blue regions between different groups (P < 0.05).CONCLUSION: Ultrasonic elastography can be used in quantitative assessment of the severity of fatty liver.

关键词： Transient elastography Ultrasonic elastography evaluation Fatty liver Quantitative diagnosis Grading of fatty liver