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动态外载荷工况下风力机法兰螺栓相对刚度研究

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风机技术 2024年第3期66卷 91-96页

作者：彭波蔡学长明仕林李严曹毅曹剑绵田大庆电子科技大学东方电气集团科学技术研究院有限公司四川大学

螺栓相对刚度决定了工作中螺栓拉力大小。为探究风力机法兰盘螺栓拉力与外载荷关系,基于法兰单元等效圆柱模型与等效压缩变形体模型,结合有限元仿真,研究了不同工况下螺栓相对刚度的变化情况。研究结果表明,螺栓相对刚度与外载荷的性质... 详细信息

螺栓相对刚度决定了工作中螺栓拉力大小。为探究风力机法兰盘螺栓拉力与外载荷关系,基于法兰单元等效圆柱模型与等效压缩变形体模型,结合有限元仿真,研究了不同工况下螺栓相对刚度的变化情况。研究结果表明,螺栓相对刚度与外载荷的性质和施加位置有关;与上下法兰单元间接触面积和螺栓初始预紧力呈负相关;动态外载荷工况下表现出明显的非线性特性。研究结论可为风力机法兰盘螺栓组设计与装配以及螺栓组松动故障预警诊断提供参考。

关键词：螺栓连接相对刚度等效压缩变形体非线性特性

Three functionally redundant plant-specific paralogs are core subunits of the SAGA histone acetyltransferase complex in Arabidopsis

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Molecular Plant 2021年第7期14卷 1071-1087页

作者： Chan-Juan Wu Zhen-Zhen Liu Long Wei Jin-Xing Zhou xue-Wei cai Yin-Na Su Lin Li She Chen Xin-Jian He College of Life Sciences Beijing Normal UniversityBeijingChina National Institute of Biological Sciences Beijing 102206China Tsinghua Institute of Multidisciplinary Biomedical Research Tsinghua UniversityBeijing 10084China

The SAGA(Spt-Ada-Gcn5 acetyltransferase)complex is an evolutionarily conserved histone acetyltransferase complex that has a critical role in histone acetylation,gene expression,and various developmental processes in ***,little is known about the composition and function of the SAGA complex in *** this study,we found that the SAGA complex in Arabidopsis thaliana contains not only conserved subunits but also four plant-specific subunits:three functionally redundant paralogs,SCSI,SCS2A,and SCS2B(SCS1/2A/2B),and a TAF-like subunit,*** in SCS1/2A/2B lead to defective phenotypes similar to those caused by mutations in the genes encoding conserved SAGA subunits HAG1 and ADA2B,including delayed juvenile-to-adult phase transition,late flowering,and increased trichome ***,we demonstrated that SCS1/2A/2B are required for the function of the SAGA complex in histone acetylation,thereby promoting the transcription of development-related *** results together suggest that SCS1/2A/2B are core subunits of the SAGA complex in *** with SAGA complexes in other eukaryotes,the SAGA complexes in plants have evolved unique features that are necessary for normal growth and development.

关键词： histone acetylation SAGA development SCSI HAG1 ADA2B

负载黄芩素的双网络水凝胶用于创面愈合的研究

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哈尔滨医科大学学报 2024年第2期58卷 129-134页

作者：冯钰钦秦思李华润施思漫段忆雪许洁如蔡雪欣温诗瑜孙大炜温炬南方医科大学第二临床医学院暨南大学附属广东省第二人民医院皮肤性病科暨南大学附属广东省第二人民医院创伤骨科

目的构建负载黄芩素的双网络水凝胶并对其促进创面愈合的机制进行研究。方法将黄芩素(baicalein)、聚乙二醇二丙烯酸酯(polyethylene glycol diacrylate, PEGDA)、羧甲基壳聚糖(carboxymethyl chitosan, CMCS)等材料，通过紫外光交联... 详细信息

目的构建负载黄芩素的双网络水凝胶并对其促进创面愈合的机制进行研究。方法将黄芩素(baicalein)、聚乙二醇二丙烯酸酯(polyethylene glycol diacrylate, PEGDA)、羧甲基壳聚糖(carboxymethyl chitosan, CMCS)等材料，通过紫外光交联及浸泡等方式，构建负载黄芩素的双网络水凝胶(PEGDA/CMCS@B),将实验分为PEGDA/CMCS、PEGDA/CMCS@B0.5和PEGDA/CMCS@B1.0 3组，运用扫描电子显微镜(scanning electron microscopy, SEM)观察其形态。选用大肠杆菌(***)和金黄色葡萄球菌(***)两种细菌，采用抑菌圈实验探究双网络水凝胶的抑菌性能。将L929细胞与3种不同水凝胶共培养48 h,运用活/死染色试验和CCK-8检测，验证水凝胶的细胞相容性。在大鼠背部建立全皮层缺损创面模型，通过不同材料治疗，运用Image J软件分析15天创面修复情况。结果 SEM结果显示，PEGDA/CMCS@B双网络水凝胶具有丰富的孔隙结构。抑菌实验证明PEGDA/CMCS@B水凝胶具有更为出色的抑菌作用(P<0.001)。大鼠全层创面模型实验证实PEGDA/CMCS@B水凝胶可显著促进创面愈合。结论负载黄芩素的双网络水凝胶可有效促进创面愈合。

关键词：黄芩素水凝胶抑菌作用创面愈合

少孢节丛孢菌几丁质酶AO-492对线虫的降解作用研究

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生物技术通报 2024年第5期40卷 261-268页

作者：张嘉华张慧梅马喜喜孙焱森李若冰李柠杏才学鹏乔军孟庆玲石河子大学动物科技学院石河子832003 中国兽医药品监察所北京100081

【目的】为探究捕食线虫真菌少孢节丛孢菌几丁质诱导过程中分泌蛋白AO-492的生物学功能。【方法】对少孢节丛孢菌几丁质酶AO-492主要结构域编码区进行基因克隆及分子特征分析,并在毕赤酵母中进行表达。利用镍柱亲和层析法纯化重组蛋白Re... 详细信息

【目的】为探究捕食线虫真菌少孢节丛孢菌几丁质诱导过程中分泌蛋白AO-492的生物学功能。【方法】对少孢节丛孢菌几丁质酶AO-492主要结构域编码区进行基因克隆及分子特征分析,并在毕赤酵母中进行表达。利用镍柱亲和层析法纯化重组蛋白ReAO-Z492,采用NAG检测法分析了该重组蛋白在不同温度、pH及金属离子条件下的酶学活性,并将其作用于秀丽隐杆线虫及虫卵分析其生物学功能。【结果】几丁质酶AO-492有信号肽,无跨膜结构域,含有两个几丁质结合结构域和一个糖苷水解酶18家族结构域,并含有糖苷水解酶18家族几丁质酶高度保守的底物结合位点-SVGGWT-和水解酶活性位点-FDGGDLDWE-,含有典型的TIM桶形分子结构。系统进化分析显示,该蛋白与坚粘孢单顶孢几丁质酶(EPS35099.1)的亲缘关系相对最近。SDS-PAGE和Western Blot分析表明,ReAO-Z492分子量约为59 kD,可与小鼠抗少孢节丛孢菌多克隆抗体发生特异性反应。ReAO-Z492最适温度为40℃,最适pH为7.0;Mg^(2+)对其酶活有促进作用,而Ag^(+)、Cu^(2+)、Fe^(3+)和Zn^(2+)有抑制作用。ReAO-Z492对秀丽隐杆线虫体壁及其虫卵卵壳有较强的降解活性。【结论】少孢节丛孢菌几丁质酶 AO-492 对秀丽隐杆线虫及虫卵具有较强的降解作用。

关键词：少孢节丛孢菌几丁质酶 ReAO-Z492 酶学活性捕食线虫真菌

Erythromycin Resistance of Streptococcus suis Isolates In Vivo

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Agricultural Sciences in China 2009年第4期8卷 502-507页

作者： JIANG Cheng-gang LI Yan-hua cai xue-hui QIU Hua-ji TONG Heng-min LIU Di-qiu LEI Lian-cheng SUN Chang-jiang Division of Swine Infectious Diseases National Key Laboratory of Veterinary Biotechnology Harbin Veterinary Research Institute Chinese Academy of Agricultural Sciences Harbin 150001 P.R. China Northeast Agricultural University Harbin 150030 P.R.China Husbandry and Veterinary Institute of Jilin University Changchun 130062 P.R.China

In order to study erythromycin resistance of Streptococcus suis under high or low concentration of selective drug pressure, Streptococcus suis strain LN was isolated from a diseased pig in 2005 and showed to be susceptible to erythromycin as determined by disc diffusion and tube dilution tests. In this study, clean level rabbits were divided into three groups of six rabbits each, including a prevention dosage group, a treatment dosage group, and a control group. After injection with S. suis strain LN, erythromycin （20 μg mL^-1） was taken orally in the prevention dosage group, erythromycin （5 mg kg^-1） was injected intramuscularly in the treatment dosage group, and no treatment was given in the control group. S. suis with intermediate resistance to erythromycin was isolated on the 5th day after infection from the prevention dosage group （5th PDG） and on the 7th day after infection from the treatment dosage group （7th TDG）. Both isolates were determined to be the constitutive macrolide-lincosamide-streptogramin B （cMLSB） resistance phenotype. The resistance gene ermB was detected in all of the isolates. The results suggested that both the 5th PDG and 7th TDG isolates had a mutation （A2372T） in the 23S rRNA gene. In addition, the 5th PDG isolates had a mutation in ribosomal protein L4 （detected as G268A） and a mutation in ribosomal protein L22 （A345C）; and the 7th TDG isolates had a C insertion at site 564. Each of these mutations is considered as a possible mechanism of erythromycin resistance in S. suis strain LN. This study demonstrated that erythromycin resistance was readily induced in S. suis at a low erythromycin dose creating selective pressure in vivo. Resistance appeared to be mediated by ribosome methylation, encoded by the ermB gene.

关键词： Streptococcus suis erythromycin in vivo resistance

An Optoelectronic thermometer based on microscale infrared-to-visible conversion devices

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Light(Science & Applications) 2022年第6期11卷 1158-1165页

作者： He Ding Guoqing Lv xue cai Junyu Chen Ziyi Cheng Yanxiu Peng Guo Tang Zhao Shi Yang Xie Xin Fu Lan Yin Jian Yang Yongtian Wang Xing Sheng Beijing Engineering Research Center of Mixed Reality and Advanced Display School of Optics and PhotonicsBeijing Institute of TechnologyBeijing 100081China Department of Electronic Engineering Beijing National Research Center for Information Science and TechnologyInstitute for Precision MedicineCenter for Flexible Electronics Technologyand IDG/McGovern Institute for Brain ResearchTsinghua UniversityBeijing 100084China School of Materials Science and Engineering Tsinghua UniversityBeijing 100084China

Thermometric detectors are crucial in evaluating the condition of target objects spanning from environments to the human ***-based thermal sensing tools have received extensive attention,in which the photon upconversion process with low autofluorescence and high tissue penetration depth is considered as a competent method for temperature monitoring,particularly in biomedical ***,we present an optoelectronic thermometer via infrared-to-visible upconversion,accomplished by integrated light receiving and emission *** fabricated thin-film,microscale devices present temperature-dependent light emission with an intensity change of 1.5%℃^(-1) and a spectral shift of 0.18 nm℃^(-1).The sensing mechanism is systematically characterized and ascribed to temperature dependent optoelectronic properties of the semiconductor band structure and the circuit operation *** device arrays showcase the capability for spatially resolved temperature ***,in vitro and in vivo experiments implemented with integrated fiber-optic sensors demonstrate real-time thermal detection of dynamic human activity and in the deep brain of animals,respectively.

关键词： visible optoelectronic thermal

Plutonium utilization in a small modular molten-salt reactor based on a batch fuel reprocessing scheme

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Nuclear Science and Techniques 2024年第4期35卷 15-28页

作者： xue-Chao Zhao Rui Yan Gui-Feng Zhu Ya-Fen Liu Jian Guo Xiang-Zhou cai Yang Zou Shanghai Institute of Applied Physics Chinese Academy of SciencesShanghai 201800China CAS Innovative Academies in TMSR Energy System Chinese Academy of SciencesShanghai 201800China University of Chinese Academy of Sciences Beijing 100049China

A molten salt reactor(MSR)has outstanding features considering the application of thorium fuel,inherent safety,sustainability,and resistance to ***,fissile material^(233)U is significantly rare at the current stage,thus it is difficult for MSR to achieve a pure thorium-uranium fuel ***,using plutonium or enriched uranium as the initial fuel for MSR is more *** this study,we aim to verify the feasibility of a small modular MSR that utilizes plutonium as the starting fuel(SM-MSR-Pu),and highlight its advantages and ***,the structural design and fuel management scheme of the SM-MSR-Pu were ***,the neutronic characteristics,such as the graphite-irradiation lifetime,burn-up performance,and coefficient of temperature reactivity were calculated to analyze the physical characteristics of the *** results indicate that plutonium is a feasible and advantageous starting fuel for a SM-MSR;however,there are certain shortcomings that need to be *** a 250 MWth SM-MSR-Pu,approximately 288.64 kg^(233)U of plutonium with a purity of greater than 90% is produced while 978.00 kg is burned every ten *** temperature reactivity coefficient decreases from -4.0 to -6.5 pcm K^(-1) over the 50-year operating time,which ensures a long-term safe ***,the amount of plutonium and accumulation of minor actinides(MAs)would increase as the burn-up time increases,and the annual production and purity of^(233)U will *** achieve an optimal burn-up performance,setting the entire operation time to 30 years is ***,more than 3600 kg of plutonium eventually accumulate in the *** research is required to effectively utilize this accumulated plutonium.

关键词： Molten salt fuel Plutonium utilization ^(233)U TRUs mole fraction Temperature feedback coefficient

Sequence and phylogenetic analysis of chicken reoviruses in China

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Journal of Integrative Agriculture 2016年第8期15卷 1846-1855页

作者： WEN Chu ZHONG Qi ZHANG Jia-dong LU Jian-shan ZHANG Li-xin YUAN Xi-min GAN Meng-hou cai xue-peng ZHANG Guo-zhong Key Laboratory of Animal Epidemiology and Zoonosis Ministry of Agriculture/College of Veterinary Medicine China Agricultural University Beijing 100193 P.R. China Beijing Kang Mu Animal Health Products and Veterinary Apparatus Supply Center Beijing 100085 P.R. China China Institute of Veterinary Drug Control Beijing 100081 P.R. China

supported by the China Animal Disease Prevention and Control Center;the China Agriculture Research System Poultry-Related Science and Technology Innovation Team of Peking, China （CARS-PSTP）

关键词： avian reovirus chicken genome S1 homology phylogenetic analysis pathogenicity

肩袖修补术患者康复锻炼依从性影响因素及护理干预研究进展

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现代医药卫生 2024年第11期40卷 1922-1925,1931页

作者：邵秒彭莉钟琦(综述) 李昕华谢樱姿蔡雪徐翠荣(审校) 东南大学医学院江苏南京210009 南京大学医学院附属鼓楼医院运动医学与成人重建外科江苏南京210008 东南大学附属中大医院护理部江苏南京210009

肩袖修补术患者康复锻炼依从性对肩关节功能恢复至关重要,但现状不够理想。通过检索国内外数据库文献,该文总结了肩袖修补术患者康复锻炼依从性的现状,分析患者生理心理因素、康复锻炼负荷、社会支持、医疗支持因素对肩袖修补术患者康... 详细信息

肩袖修补术患者康复锻炼依从性对肩关节功能恢复至关重要,但现状不够理想。通过检索国内外数据库文献,该文总结了肩袖修补术患者康复锻炼依从性的现状,分析患者生理心理因素、康复锻炼负荷、社会支持、医疗支持因素对肩袖修补术患者康复锻炼依从性的影响并阐释相关护理干预模式,旨在为提高肩袖修补术患者康复锻炼依从性提供参考依据。

关键词：肩袖损伤康复锻炼依从性影响因素护理干预综述