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Bone-targeting engineered small extracellular vesicles carrying anti-miR-6359-CGGGAGC prevent valproic acid-induced bone loss

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Signal Transduction and Targeted Therapy 2024年第2期9卷 751-765页

作者： Xudong Xie Peng Cheng Liangcong Hu Wu Zhou Detai Zhang Samuel Knoedler Guodong Liu Yuan Xiong hang xue Yiqiang Hu Barbara Kern Doha Obed Adriana CPanayi Lang Chen Chenchen Yan Ze Lin Guandong Dai Bobin Mi Yingze Zhang Guohui Liu Department of Orthopedics Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China Hubei Province Key Laboratory of Oral and Maxillofacial Development and Regeneration Wuhan 430022China Department of Clinical Laboratory Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei 430022P.R.China Division of Plastic Surgery Brigham and Women’s HospitalHarvard Medical SchoolBostonMA 02152USA Department of Plastic Surgery and Hand Surgery Klinikum Rechts der IsarTechnical University of MunichMunichGermany Medical Center of Trauma and War Injuries Daping HospitalArmy Medical UniversityChonqing 400042China Department of Plastic Surgery Campus CharitéMitte|Campus Virchow-KlinikumCharité-Universitätsmedizin Berlincorporate member of Freie Universität BerlinHumboldt-Universität zu Berlin and Berlin Institute of HealthBerlinGermany Berlin Institute of Health Charité-Universitätsmedizin BerlinBerlinGermany Department of Plastic AestheticHand and Reconstructive SurgeryHannover Medical SchoolHannoverGermany Department of Hand Plastic and Reconstructive SurgeryMicrosurgeryBurn CenterBG Trauma Center LudwigshafenUniversity of HeidelbergLudwig-Guttmann-Strasse 1367071 Ludwigshafen/RhineGermany Pingshan District People’s Hospital of Shenzhen Pingshan General Hospital of Southern Medical UniversityShenzhenGuangdong 518118China Department of Orthopaedic Surgery The Third Hospital of Hebei Medical UniversityNO.139 Ziqiang RoadShijiazhuang 050051China

The clinical role and underlying mechanisms of valproic acid(VPA)on bone homeostasis remain ***,we confirmed that VPA treatment was associated with decreased bone mass and bone mineral density(BMD)in both patients and *** effect was attributed to VPA-induced elevation in osteoclast formation and *** RNA-sequencing,we observed a significant rise in precursor miR-6359 expression in VPA-treated osteoclast precursors in vitro,and further,a marked upregulation of mature miR-6359(miR-6359)in vivo was demonstrated using quantitative real-time PCR(qRT-PCR)and miR-6359 fluorescent in situ hybridization(miR-6359-FISH).Specifically,the miR-6359 was predominantly increased in osteoclast precursors and macrophages but not in neutrophils,T lymphocytes,monocytes and bone marrow-derived mesenchymal stem cells(BMSCs)following VPA stimulation,which influenced osteoclast differentiation and bone-resorptive ***,VPA-induced miR-6359 enrichment in osteoclast precursors enhanced reactive oxygen species(ROS)production by silencing the SIRT3 protein expression,followed by activation of the MAPK signaling pathway,which enhanced osteoclast formation and activity,thereby accelerating bone ***,there are no medications that can effectively treat VPA-induced bone ***,we constructed engineered small extracellular vesicles(E-sEVs)targeting osteoclast precursors in bone and naturally carrying anti-miR-6359 by introducing of EXOmotif(CGGGAGC)in the 3’-end of the anti-miR-6359 *** confirmed that the E-sEVs exhibited decent bone/osteoclast precursor targeting and exerted protective therapeutic effects on VPA-induced bone loss,but not on ovariectomy(OVX)and glucocorticoid-induced osteoporotic models,deepening our understanding of the underlying mechanism and treatment strategies for VPA-induced bone loss.

关键词： miR protective stimulation

七氟醚后处理对缺血缺氧性脑损伤新生大鼠远期脑白质MBP表达的影响

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中华麻醉学杂志 2020年第9期40卷 1132-1135页

作者：薛杭吴子怡赵平中国医科大学附属盛京医院麻醉科沈阳110004

目的评价七氟烷后处理对缺血缺氧性脑损伤新生大鼠远期脑白质髓鞘碱性蛋白(MBP)表达的影响。方法SPF级健康SD大鼠48只,7日龄,体重13~17 g,雌雄各半,采用随机数字表法分为3组(n=16):对照组(Sham组)、缺血缺氧性脑损伤组(HIBI组)和缺血缺... 详细信息

目的评价七氟烷后处理对缺血缺氧性脑损伤新生大鼠远期脑白质髓鞘碱性蛋白(MBP)表达的影响。方法SPF级健康SD大鼠48只,7日龄,体重13~17 g,雌雄各半,采用随机数字表法分为3组(n=16):对照组(Sham组)、缺血缺氧性脑损伤组(HIBI组)和缺血缺氧性脑损伤组+七氟烷后处理(HIBI+S组)。采用结扎左颈总动脉方法制备缺血缺氧性脑损伤模型。于缺氧结束时即刻吸入2.5%七氟烷湿化混合气体30 min进行七氟烷后处理。于缺血缺氧性脑损伤模型制备后第29-34天进行Morris水迷宫实验。于缺血缺氧性脑损伤模型制备后35 d时,采用免疫组化法测脑白质MBP表达水平。结果与Sham组比较,HIBI组逃避潜伏期延长,目标象限停留时间缩短,穿越原平台次数减少,脑白质MBP表达下调(P<0.01或0.05);与HIBI组比较,HIBI+S组逃避潜伏期缩短,目标象限停留时间延长,穿越原平台次数增多,脑白质MBP表达上调(P<0.01或0.05)。结论七氟烷后处理减轻缺血缺氧性脑损伤新生大鼠远期认知功能障碍的机制可能与上调脑白质MBP表达有关。

关键词：髓鞘碱性蛋白麻醉药,吸入缺血后处理缺氧缺血,脑婴儿,新生

基于对比学习的高价值发明专利识别——以无线通信网络领域为例

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情报杂志 2024年第9期43卷 179-187页

作者：薛航施国良陈挺河海大学商学院南京211100

[研究目的]在众多专利中准确高效识别高价值发明专利,不仅对中国知识产权战略实施具有推动作用,还有助于促进高价值发明专利的技术转化。[研究方法]针对领域专利文本利用不充分的问题,对使用Bert在无线通信网络领域专利文本上进行对比... 详细信息

[研究目的]在众多专利中准确高效识别高价值发明专利,不仅对中国知识产权战略实施具有推动作用,还有助于促进高价值发明专利的技术转化。[研究方法]针对领域专利文本利用不充分的问题,对使用Bert在无线通信网络领域专利文本上进行对比学习预训练,得到领域适应的Bert模型。然后,利用领域适应的Bert模型训练高价值发明专利识别模型,并在高价值发明专利识别模型的训练过程中使用过采样策略缓解正负样本不均衡的问题,改善模型的效果。[研究结论]在包含62 000份无线通信网络中国发明专利数据集上的实验结果显示,使用对比学习和过采样策略训练得到的模型在Accuracy指标值和Macro-F1指标值上分别达到了97%和0.93,相比于直接使用Bert分别提升了9.77%和0.19。

关键词：高价值发明专利专利识别专利文本专利价值评估对比学习无线通信网络

一种通过约瑟夫森结非线性频率响应确定微波耗散的方法

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物理学报 2019年第11期68卷 263-268页

作者：陈恒杰薛航李邵雄王镇中国科学院上海微系统与信息技术研究所信息功能材料国家重点实验室上海200050 中国科学院超导电子学卓越创新中心上海200050 中国科学院大学北京100049

通过对电流偏置超导约瑟夫森结的微波驱动行为的研究,提出了一个确定约瑟夫森结微波耗散的方法.结的微波耗散由它的品质因子描述.微波耗散严重影响约瑟夫森器件如参量放大器、超导量子比特等的性能.对电流偏置的约瑟夫森结势阱采用四阶... 详细信息

通过对电流偏置超导约瑟夫森结的微波驱动行为的研究,提出了一个确定约瑟夫森结微波耗散的方法.结的微波耗散由它的品质因子描述.微波耗散严重影响约瑟夫森器件如参量放大器、超导量子比特等的性能.对电流偏置的约瑟夫森结势阱采用四阶近似后,可以得到在较强微波驱动下约瑟夫森结非线性微波响应方程.该方程定量描述了非线性共振频率随外加微波功率变化关系:非线性共振频率与结等离子频率的差别依赖于约瑟夫森结的微波品质因子.对电流偏置的约瑟夫森结的微波运动行为进行了数值模拟.模拟结果确证了微波品质因子与非线性共振频率-等离子频率差别的定量关系可以应用于约瑟夫森结中.用这种非线性频率响应方法来确定约瑟夫森结的微波耗散没有严格的温度要求,可在单个电流偏置的结中完成,实验上具有简单可靠性.

关键词：约瑟夫森结非线性共振品质因子超导量子比特

Hypoxia endothelial cells-derived exosomes facilitate diabetic wound healing through improving endothelial cell function and promoting M2 macrophages polarization

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Bioactive Materials 2024年第3期33卷 157-173页

作者： Peng Cheng Xudong Xie Liangcong Hu Wu Zhou Bobin Mi Yuan Xiong hang xue Kunyu Zhang Yuxiao Zhang Yiqiang Hu Lang Chen Kangkang Zha Bin Lv Ze Lin Chuanlu Lin Guandong Dai Yixin Hu Tengbo Yu Hankun Hu Guohui Liu Yingze Zhang Department of Orthopedics Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430022China Hubei Province Key Laboratory of Oral and Maxillofacial Development and Regeneration Wuhan430022China School of Biomedical Sciences and Engineering South China University of TechnologyGuangzhou International CampusGuangzhou511442China Department of Orthopaedics Pingshan District People’s Hospital of ShenzhenPingshan General Hospital of Southern Medical UniversityShenzhenGuangdong518118China Hubei Micro-explore Innovative Pharmaceutical Research Co LtdWuhanHubei430071China Suzhou Organ-on-a-Chip System Science and Technology Co LtdSuzhouJiangsu215000China Department of Sports Medicine The Affiliated Hospital of Qingdao UniversityQingdaoChina Department of Pharmacy Zhongnan Hospital of Wuhan UniversitySchool of Pharmaceutical SciencesWuhan UniversityWuhan430071China Department of Orthopaedic Surgery The Third Hospital of Hebei Medical UniversityNO.139 Ziqiang RoadShijiazhuang050051China

It is imperative to develop and implement newer,more effective strategies to address refractory diabetic *** of now,there is currently no optimal solution for these *** human umbilical vein endothelial cells(HUVECs)-derived exosomes have been postulated to promote diabetic wound healing,however,its effect and molecular mechanism need further *** this study,we aimed to investigate whether hypoxic exosomes enhance wound healing in *** on our high-throughput sequencing,differentially expressed lncRNAs(including 64 upregulated lncRNAs and 94 downregulated lncRNAs)were found in hypoxic exosomes compared to normoxic ***,lncHAR1B was one of the prominently upregulated lncRNAs in hypoxic exosomes,showing a notable correlation with diabetic wound *** specifically,hypoxic exosomes were transmitted to surrounding cells,which resulted in a significant increase in lncHAR1B level,thereby relieving the dysfunction of endothelial cells and promoting the switch from M1 to M2 macrophages under high glucose ***,lncHAR1B directly interacted with the transcription factor basic helix-loop-helix family member e23(BHLHE23),which subsequently led to its binding to the KLF transcription factor 4(KLF4)and promoted KLF4 *** our in vivo experiments,the use of hypoxic exosomes-loaded HGM-QCS hydrogels(Gel-H-Exos)resulted in rapid wound healing compared to that of normoxic exosomes-loaded HGM-QCS hydrogels(Gel-N-Exos)and diabetic ***,our study provides potentially novel therapeutic approaches aimed at accelerating wound healing and developing a practical exosomes delivery platform.

关键词： Diabetic wound Hypoxic exosomes lncHAR1B KLF4 Macrophage polarization

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基于光纤光栅的物体表面附近温度场测量

光子学报 2014年第5期43卷 1-4页

作者：余有龙薛航王彦晓段营营合肥工业大学仪器科学与光电工程学院合肥230009

提出一种利用光纤布喇格光栅温度传感器测量物体表面法向局域内温度场分布的新方法.将多个毛细管封装的波分复用光纤光栅温度传感器串接,沿待测位置法线方向平行于表面等间距放置,对法向局域内的温度进行准分布式监测,封装后的传感器响... 详细信息

提出一种利用光纤布喇格光栅温度传感器测量物体表面法向局域内温度场分布的新方法.将多个毛细管封装的波分复用光纤光栅温度传感器串接,沿待测位置法线方向平行于表面等间距放置,对法向局域内的温度进行准分布式监测,封装后的传感器响应时间为2.74s.利用ANSYS软件对空气的热稳态层流进行分析,获得温度场分布的等值线图.实验得到温度随高度变化的一阶变化率为-24.262,二阶变化率为10.117 2,而ANSYS仿真结果给出的分别为-22.842和8.613,两者基本相符,验证了此测量方法的可靠性.该技术为了解温度场的局域分布提供了技术支撑,为航空器选材和构型的确定提供新的测试方案.

关键词：光纤光学光纤布喇格光栅波分复用温度分布传感器分布式传感流场

Exosomal PD-L1 induces osteogenic differentiation and promotes fracture healing by acting as an immunosuppressant

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Bioactive Materials 2022年第7期7卷 300-311页

作者： Ze Lin Yuan Xiong Weilin Meng Yiqiang Hu Lili Chen Lang Chen hang xue Adriana CPanayi Wu Zhou Yun Sun Faqi Cao Guodong Liu Liangcong Hu Chenchen Yan Xudong Xie Chuanchuan Lin Kaiyong Cai Qian Feng Bobin Mi Guohui Liu Department of Orthopaedics Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430022China Hubei Province Key Laboratory of Oral and Maxillofacial Development and Regeneration Wuhan430022China Key Laboratory of Biorheological Science and Technology Ministry of EducationCollege of BioengineeringChongqing UniversityChongqing400044China Division of Plastic Surgery Brigham and Women’s HospitalHarvard Medical SchoolBostonMA02152USA Department of Neurosurgery Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430022China Medical Center of Trauma and War Injuries Daping HospitalArmy Medical UniversityChongqing400042China Department of Stomatology Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430022China

A moderate inflammatory response at the early stages of fracture healing is necessary for callus ***-active and continuous inflammation,however,impairs fracture healing and leads to excessive tissue *** fracture healing could be promoted through suppression of local over-active immune cells in the fracture *** the present study,we achieved an enriched concentration of PD-L1 from exosomes(Exos)of a genetically engineered Human Umbilical Vein Endothelial Cell(HUVECs),and demonstrated that exosomes overexpressing PD-L1 specifically bind to PD-1 on the T cell surface,suppressing the activation of T ***,exosomal PD-L1 induced Mesenchymal Stem Cells(MSCs)towards osteogenic differentiation when pre-cultured with T ***,embedding of Exos into an injectable hydrogel allowed Exos delivery to the surrounding microenvironment in a time-released ***,exosomal PD-L1,embedded in a hydrogel,markedly promoted callus formation and fracture healing in a murine model at the early over-active inflammation ***,our results suggested that activation of T cells in the peripheral lymphatic tissues was inhibited after local administration of PD-L1-enriched Exos to the fracture sites,while T cells in distant immune organs such as the spleen were not *** summary,this study provides the first example of using PD-L1-enriched Exos for bone fracture repair,and highlights the potential of Hydrogel@Exos systems for bone fracture therapy through immune inhibitory effects.

关键词： PD-L1 Exosome Fracture healing Hydrogel Immunotherapy

全身麻醉复合硬膜外阻滞对卵巢癌围手术期镇痛和血流动力学及免疫功能的影响

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中国肿瘤临床与康复 2022年第12期29卷 1437-1441页

作者：薛行行薛蓉陕西省榆林市第一医院麻醉科榆林718000

目的探讨全身麻醉复合硬膜外阻滞对卵巢癌围手术期镇痛、血流动力学及免疫功能的影响。方法选取2019年5月至2021年2月间榆林市第一医院收治的106例卵巢癌患者为研究对象,采用随机数表法将其分为复合组与对照组,每组53例。两组患者均行... 详细信息

目的探讨全身麻醉复合硬膜外阻滞对卵巢癌围手术期镇痛、血流动力学及免疫功能的影响。方法选取2019年5月至2021年2月间榆林市第一医院收治的106例卵巢癌患者为研究对象,采用随机数表法将其分为复合组与对照组,每组53例。两组患者均行卵巢癌根治术治疗,对照组给予全身麻醉,复合组给予全身麻醉联合硬膜外阻滞。对比两组手术相关指标(术中出血量、手术时间以及住院时间),比较术前(T)、麻醉后30min(T)、术毕(T)、术后2 h(T)两组视觉模拟评分(VAS)、平均动脉压(MAP)、心率(HR)、CD3^(+)、CD4^(+)水平,记录两组术后3 d内不良反应发生情况。结果两组术中出血量、手术时间比较,差异无统计学意义(P>0.05)。复合组住院时间短于对照组,差异有统计学意义(P0.05)。两组T时刻VAS评分低于T、T、T时刻;两组T时刻VAS评分低于T、T时刻;两组T时刻VAS评分低于T时刻;且复合组T、T、T时刻VAS评分低于对照组,差异均有统计学意义(均P0.05)。两组T时刻MAP高于T时刻,低于T、T时刻;两组T时刻MAP高于T、T时刻;两组T时刻MAP高于T时刻,差异均有统计学意义(均P0.05)。两组T时刻CD3^(+)、CD4^(+)低于T、T、T时刻;两组T时刻CD3^(+)、CD4^(+)低于T、T时刻;两组T时刻CD3^(+)、CD4^(+)低于T时刻;且复合组T、T、T时刻CD3^(+)、CD4^(+)高于对照组,差异均有统计学意义(均P0.05)。结论全身麻醉复合硬膜外阻滞用于卵巢癌根治术,可缩短住院时间,减轻围手术期疼痛,稳定血流动力学,对免疫功能的影响较小,且安全可靠。

关键词：全身麻醉硬膜外阻滞卵巢肿瘤围手术期镇痛血流动力学免疫功能

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纳米Y型分子筛的合成研究进展

现代化工 2022年第6期42卷 25-29页

作者：薛景航秦波高杭柳伟中国石化大连石油化工研究院辽宁大连116045

综述了近年来纳米Y型分子筛的合成方法,包括无导向剂合成法、晶种辅助晶化法、硬模板剂法、软模版剂法和有机添加剂法,并对这些方法的优缺点进行了评价。讨论了纳米Y分子筛合成目前存在的问题,对纳米Y分子筛未来发展进行了展望,指出深... 详细信息

综述了近年来纳米Y型分子筛的合成方法,包括无导向剂合成法、晶种辅助晶化法、硬模板剂法、软模版剂法和有机添加剂法,并对这些方法的优缺点进行了评价。讨论了纳米Y分子筛合成目前存在的问题,对纳米Y分子筛未来发展进行了展望,指出深入研究纳米Y分子筛的晶化机理、提高纳米Y分子筛的结晶度和骨架硅铝比是今后的研究重点。

关键词： Y分子筛纳米晶合成