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Stability improvement of human collagenα1(I)chain using insulin as a fusion partner

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Chinese Journal of Chemical Engineering 2018年第12期26卷 2607-2614页

作者： Yu Mi Yuan Gao Daidi Fan Zhiguang Duan Rongzhan Fu Lihua Liang wenjiao xue Shanshan Wang Shaanxi R&D Center of Biomaterials and Fermentation Engineering School of Chemical Engineering Northwest UniversityXi'an 710069China Shaanxi Institute of Microbiology Xi'an 710043China School of Biological Science and Engineering Shaanxi University of Technology Hanzhong 723001China

To enhance the stability of recombinant human collagen α1(I) chains(rhCOL1 A1) in production and purification stages, a gene fragment fusing COL1 A1 and insulin protein coding domains was synthesized and inserted into the pPIC9 K expression vector. The fusion peptide-expressing Pichia pastoris strain was created by *** optimization of shake flask cultures, the ultimate intracellular expression level of the insulin-collagen α1(I) chain fusion protein(INS-COL1 A1) reached about 300 mg·L^(-1), and no obvious protein degradation was found in the fermentation and purification processes. The His-tagged recombinant fusion protein was detected by western blotting and was effectively purified using Ni^(2+)-chelating chromatography. A prominent improvement in the stability of INS-COL1 A1 was observed compared to rhCOL1 A1 in vitro, and the rhCOL1 A1 released from the fusion protein was studied by LC–MS/MS and in bioassays. The results showed that the purified rhCOL1 A1 was consistent with the native protein in amino acid composition and had a similar biological compatibility. To our knowledge, this is the first study to demonstrate the use of insulin as a fusion protein to improve the stability of easily degradable proteins.

关键词： Fusion expression Human collagen α1(I) chain Insulin Protein stability Pichia pastoris

白酒酿造副产物黄水中产酯酵母菌株分离鉴定及产酯条件优化

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现代农业科技 2024年第12期 154-159,166页

作者：申旻陈雅楠刘瑶刘晨朱晨辉薛文娇陕西省太白酒业有限责任公司陕西宝鸡722300 陕西省微生物研究所陕西西安710043 西北大学陕西西安710069

利用三丁酸甘油酯法,从太白酒酿造副产物黄水中分离筛选出产酯能力相对较高的酵母菌株,并对最高酶活菌株进行16S rDNA鉴定,结果确定其为异威克汉逊酵母(Wickerhamomyces anomalus)。根据单因素试验结果,结合Box-Behnken Design-响应面... 详细信息

利用三丁酸甘油酯法,从太白酒酿造副产物黄水中分离筛选出产酯能力相对较高的酵母菌株,并对最高酶活菌株进行16S rDNA鉴定,结果确定其为异威克汉逊酵母(Wickerhamomyces anomalus)。根据单因素试验结果,结合Box-Behnken Design-响应面试验优化该菌株的培养条件。结果表明,当初始乙醇体积分数为3%、发酵pH值为5.5、发酵时间为48 h、发酵温度为34℃时,该菌株能继续生长,且酯化能力明显增强,总酯含量从4.1330 g/L提高至9.9100 g/L,其中代表性组分乙酸乙酯的相对含量从41.7200 mg/100 mL提高到302.1200 mg/100 mL。

关键词：白酒黄水产酯酵母分离鉴定酯化能力条件优化

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Research progress in assessment and strategies for sustainable food system within planetary boundaries

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Science China Earth Sciences 2024年第2期67卷 375-386页

作者： Minglei WANG wenjiao SHI Key Laboratory of Land Surface Pattern and Simulation Institute of Geographic Sciences and Natural Resources ResearchChinese Academy of SciencesBeijing100101China College of Resources and Environment University of Chinese Academy of SciencesBeijing100049China

Meeting the increasing food demand in a manner that ensures both resources and environmental sustainability poses a global *** conceptual framework of planetary boundaries(PBs)has emerged as a crucial reference in the study of sustainable food system,with specific focuses on key dimensions such as land use change,freshwater use,nitrogen(N)and phosphorus(P)cycles,and greenhouse gas(GHG)*** this study,we summarized the threshold applications of PBs in sustainable food system research at both the global and national(China)*** on these thresholds,we evaluated the sustainability conditions of the food system,considering resources such as cropland,freshwater,N and P applications,as well as environmental impacts including N and P losses,and GHG *** addition,we explored the impacts of single or combined management strategies on sustainable food *** strategies included dietary changes,improvements in technologies and management,reductions in food loss and waste,and optimization in agricultural production ***,we outlined future research directions in sustainable food system,including enhancing research on the interaction mechanisms among PBs elements within food systems,conducting downscaling studies of PBs elements at national and regional levels,and ensuring the rationality of policy-making for sustainable food systems in *** study can provide a theoretical foundation and strategies guidance for sustainable food system and agricultural land use management in the future both globally and in China.

关键词： Food system Resource use Environmental impacts Sustainable strategy Planetary boundaries

A DIRECT DISCONTINUOUS GALERKIN METHOD FOR TIME FRACTIONAL DIFFUSION EQUATIONS WITH FRACTIONAL DYNAMIC BOUNDARY CONDITIONS

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Journal of Computational Mathematics 2024年第1期42卷 156-177页

作者： Jingjun Zhao wenjiao Zhao Yang Xu School of Mathematics Harbin Institute of TechnologyHarbin 150001China

This paper deals with the numerical approximation for the time fractional diffusion problem with fractional dynamic boundary *** well-posedness for the weak solutions is studied.A direct discontinuous Galerkin approach is used in spatial direction under the uniform meshes,together with a second-order Alikhanov scheme is utilized in temporal direction on the graded mesh,and then the fully discrete scheme is ***,the stability and the error estimate for the full scheme are analyzed in *** experiments are also given to illustrate the effectiveness of the proposed method.

关键词： Time fractional diffusion equation Numerical stability Convergence

A new scheme of PM_(2.5)and O_(3) control strategies with the integration of SOM,GA and WRF-CAMx

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Journal of Environmental Sciences 2024年第4期138卷 249-265页

作者： wenjiao Duan Xiaoqi Wang Shuiyuan Cheng Ruipeng Wang Key Laboratory of Beijing on Regional Air Pollution Control Faculty of Environment and LifeBeijing University of TechnologyBeijing 100124China

Previous air pollution control strategies didn’t pay enough attention to regional collaboration and the spatial response sensitivities,resulting in limited control effects in *** study proposed an effective PM_(2.5)and O_(3) control strategy scheme with the integration of Self-Organizing Map(SOM),Genetic Algorithm(GA)and WRF-CAMx,emphasizing regional collaborative control and the strengthening of control in sensitive *** scheme embodies the idea of hierarchical management and spatial-temporally differentiated management,with SOM identifying the collaborative subregions,GA providing the optimized subregion-level priority of precursor emission reductions,and WRF-CAMx providing response sensitivities for grid-level priority of precursor emission *** Beijing-Tianjin-Hebei and the surrounding area(BTHSA,“2+26”cities)as the case study area,the optimized strategy required that regions along Taihang Mountains strengthen the emission reductions of all precursors in PM_(2.5)-dominant seasons,and strengthen VOCs reductions but moderate NOx reductions in O_(3)-dominant *** spatiotemporally differentiated control strategy,without additional emission reduction burdens than the 14th Five-Year Plan proposed,reduced the average annual PM_(2.5)and MDA8 O_(3) concentrations in 28 cities by 3.2%-8.2% and 3.9%-9.7% respectively in comparison with non-differential control strategies,with the most prominent optimization effects occurring in the heavily polluted seasons(6.9%-18.0%for PM_(2.5)and 3.3%-14.2% for MDA8 O_(3),respectively).This study proposed an effective scheme for the collaborative control of PM_(2.5)and O_(3) in BTHSA,and shows important methodological implications for other regions suffering from similar air quality problems.

关键词： PM_(2.5)and O_(3)control SOM GA WRF-CAMx Bionic algorithm Machine learning

Progress in models for coupled human and natural systems

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Science China Earth Sciences 2024年第11期67卷 3631-3637页

作者： Tianxiang YUE Chenchen WU wenjiao SHI Yongzhong TIAN Qing WANG Yimin LU Lili ZHANG State Key Laboratory of Resources and Environment Information System Institute of Geographical Sciences and Natural Resources ResearchChinese Academy of Sciences College of Resources and Environment University of Chinese Academy of Sciences School of Geographical Sciences Southwest University National Institute of Environmental Health Chinese Center for Disease Control and Prevention National Engineering Research Center of Geospatial Information Technology Fuzhou University The Aerospace Information Research Institute Chinese Academy of Sciences

1. Introduction Malthus (1798) was one of the earliest researchers to propose a coupled human-natural system (CHANS) model, particularly in his analysis of the relationship between population growth and food supply. He argued that while population grows geometrically, food supply increases only arithmetically, suggesting that this imbalance could eventually lead to a food crisis due to diminishing per capita food availability (Malthus, 1798).

关键词： ECOLOGICAL-SYSTEMS SUSTAINABILITY POPULATION COMPLEXITY DECISIONS FRAMEWORK DYNAMICS EARTH

An Overview on the Composition and Age of Upper Crust of Proto-Tethyan Lajishan Intra-oceanic Arc,NE Tibet Plateau

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Acta Geologica Sinica(English Edition) 2024年第2期98卷 285-302页

作者： FU Changlei HE Xiaohu YAN Zhen Jonathan CAITCHISON XIAO wenjiao WANG Bingzhang LI Wufu LI Yusen SinoProbe Laboratory Institute of GeologyChinese Academy of Geological SciencesBeijing 100037China Key Laboratory of the Northern Qinghai–Tibet Plateau Geological Processes and Mineral Resources Qinghai Geological Survey InstituteXining 810012China Department of Geology School of Earth SciencesYunnan UniversityKunming 650091China School of Earth and Environmental Sciences University of QueenslandBrisbane 4072Australia Xinjiang Institute of Ecology and Geography Chinese Academy of SciencesUrumqi 830011China State Key Laboratory of Lithospheric Evolution Institute of Geology and GeophysicsChinese Academy of SciencesBeijing 100029China School of Earth Science and Resources China University of GeosciencesBeijing 100083China

Identification and anatomy of oceanic arcs within ancient orogenic belt are significant for better understanding the tectonic framework and closure process of paleo-ocean *** article summarizes the geological,geochemical,and geochronological characteristics of upper crust of Proto-Tethyan Lajishan intra-oceanic arc and provides new data to constrain the subduction evolution of the South Qilian *** intra-oceanic arc volcanic rocks,including intermediate-mafic lava,breccia,tuff,and minor felsic rocks,are distributed along southern part of the Lajishan ophiolite *** and isotopic compositions indicate that the intermediate-mafic lava were originated from depleted mantle contaminated by sediment melts or hydrous fluids,whereas the felsic rocks were likely generated by partial melting of juvenile mafic crust in intra-oceanic arc *** from felsic rocks yield consistent and concordant ages ranging from 506 to 523 Ma,suggesting these volcanic rocks represent the relicts of upper crust of the Cambrian intra-oceanic *** with the Cambrian forearc ophiolite and accretionary complex,we suggest that the Cambrian intra-oceanic arc in the Lajishan ophiolite belt is belonging to the intra-oceanic arc system which was generated by south-directed subduction in the South Qilian Ocean at a relatively short interval between approximately 530 and 480 Ma.

关键词： volcanic rocks intra-oceanic arc U-Pb age Lajishan ophiolite belt South Qilian Ocean Proto-Tethyan realm

Character Sheaves for Classical Graded Lie Algebras

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Acta Mathematica Sinica,English Series 2024年第3期40卷 870-884页

作者： Ting xue School of Mathematics and Statistics University of MelbourneVIC 3010Australia Department of Mathematics and Statistics University of HelsinkiHelsinki 00014Finland

In this note we study character sheaves for graded Lie algebras arising from inner automorphisms of special linear groups and Vinberg’s type Ⅱ classical graded Lie algebras.

关键词： Graded Lie algebras character sheaves Fourier transform nilpotent orbits

自体富血小板血浆对游离皮瓣修复兔软组织缺损的影响

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中华烧伤杂志 2019年第9期35卷 683-689页

作者：李洋郑健生王彪薛文娇解放军联勤保障部队第九〇九医院(厦门大学附属东南医院)烧伤整形科漳州363000

目的探讨自体富血小板血浆(PRP)对游离皮瓣修复兔软组织缺损的影响。方法取30只6个月龄、雌雄不拘新西兰大白兔,心脏采血后Aghaloo法制备PRP,于每只兔耳制作5 cm×3 cm的游离皮瓣模型。采用随机数字表法选择每只兔一侧兔耳作为PRP组,另... 详细信息

目的探讨自体富血小板血浆(PRP)对游离皮瓣修复兔软组织缺损的影响。方法取30只6个月龄、雌雄不拘新西兰大白兔,心脏采血后Aghaloo法制备PRP,于每只兔耳制作5 cm×3 cm的游离皮瓣模型。采用随机数字表法选择每只兔一侧兔耳作为PRP组,另一侧兔耳作为生理盐水组。PRP组兔耳皮瓣基底均匀涂抹1.0 mL自体PRP,生理盐水组涂抹等量生理盐水,皮瓣原位回植。分别于术后2、3、5、7、14 d,每组取6只兔,观察并记录兔耳皮瓣成活情况;取皮瓣组织,行苏木精-伊红染色观察皮瓣基底组织形态;采用免疫荧光法检测皮瓣基底组织的CD31和α平滑肌肌动蛋白(α-SMA)表达。另取1只同等实验条件下不制备皮瓣的6个月龄雄性新西兰大白兔,分别采集全血和制备PRP,行血小板计数及采用双抗体夹心酶联免疫吸附测定法检测血管内皮生长因子(VEGF)和转化生长因子β(TGF-β)含量。对数据行析因设计方差分析、配对样本t检验及Bonferroni校正。结果(1)术后2 d,PRP组兔创面皮瓣颜色彤红,与基底组织贴合良好;生理盐水组兔创面皮瓣颜色暗红,与基底组织贴合不良。术后3 d,PRP组兔创面皮瓣颜色红润,与基底组织贴合紧密;生理盐水组兔创面皮瓣散在斑状暗红,与基底组织贴合一般。术后5 d,PRP组兔创面皮瓣淡红,与基底组织贴合紧密,皮瓣已成活;生理盐水组兔创面皮瓣与基底组织贴合紧密,皮瓣颜色红润。术后7 d,PRP组兔创面皮瓣表面覆盖中量兔毛,皮瓣颜色与周围皮肤颜色接近;生理盐水组兔创面皮瓣与基底贴合一般,表面仅有少量绒毛覆盖。术后14 d,PRP组兔创面愈合良好,生理盐水组仍有小创面尚未愈合。(2)术后2 d,2组兔创面皮瓣均有炎性细胞浸润。术后3 d,PRP组兔创面皮瓣可见新生微血管,间质出血少;生理盐水组兔创面皮瓣新生微血管少。术后5 d,生理盐水组兔创面皮瓣中量炎性细胞浸润,新生微血管数量少;PRP组兔创面皮瓣可见大量Fb,少量炎性细胞浸润,明显可见周围散布新生微血管。术后7 d,生理盐水组兔创面皮瓣新生微血管数量较PRP组少。术后14 d,PRP组兔创面皮瓣新生微血管分化逐渐成熟,周围大量Fb分布;生理盐水组兔创面皮瓣部分新生微血管分化成熟,愈合过程较PRP组迟缓。(3)术后2、3、5、7、14 d,PRP组兔创面皮瓣基底组织的CD31和α-SMA表达量均明显多于生理盐水组(t=10.133、5.444、9.450、6.986、8.394,14.896、10.328、9.295、13.902、10.814,P<0.01)。(4)活化后的兔PRP中血小板计数为(2 863±962)×10^9/L,明显高于全血中的(393±49)×10^9/L(t=7.690,P<0.05)。(5)活化后的兔PRP中VEGF、TGF-β含量分别为(564.3±3.2)、(1 143±251)pg/mL,明显高于全血中的(99.7±0.4)、(274±95)pg/mL(t=287.390、9.648,P<0.05或P<0.01)。结论兔PRP中含有大量的VEGF与TGF-β,可以有效促进游离皮瓣组织微血管新生,加快游离皮瓣成活。

关键词：富血小板血浆外科皮瓣伤口愈合微血管密度