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Cryptic divergences and repeated hybridizations within the endangered “living fossil” dove tree(Davidia involucrata) revealed by whole genome resequencing

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Plant Diversity 2024年第2期46卷 169-180页

作者： Yumeng Ren Lushui Zhang xuchen yang Hao Lin Yupeng Sang Landi Feng Jianquan Liu Minghui Kang Key Laboratory of Bio-resource and Eco-environment of Ministry of Education College of Life SciencesSichuan UniversityChengdu 610065China State Key Laboratory of Herbage Improvement and Grassland Agro-ecosystem College of EcologyLanzhou UniversityLanzhou 730000China Guangdong Provincial Key Laboratory of Plant Adaptation and Molecular Design Guangzhou Key Laboratory of Crop Gene EditingInnovative Center of Molecular Genetics and EvolutionSchool of Life SciencesGuangzhou UniversityGuangzhou 510006China

The identification and understanding of cryptic intraspecific evolutionary units(lineages) are crucial for planning effective conservation strategies aimed at preserving genetic diversity in endangered ***, the factors driving the evolution and maintenance of these intraspecific lineages in most endangered species remain poorly understood. In this study, we conducted resequencing of 77 individuals from 22 natural populations of Davidia involucrata, a “living fossil” dove tree endemic to central and southwest China. Our analysis revealed the presence of three distinct local lineages within this endangered species, which emerged approximately 3.09 and 0.32 million years ago. These divergence events align well with the geographic and climatic oscillations that occurred across the distributional ***, we observed frequent hybridization events between the three lineages, resulting in the formation of hybrid populations in their adjacent as well as disjunct regions. These hybridizations likely arose from climate-driven population expansion and/or long-distance gene flow. Furthermore, we identified numerous environment-correlated gene variants across the total and many other genes that exhibited signals of positive evolution during the maintenance of two major local lineages. Our findings shed light on the highly dynamic evolution underlying the remarkably similar phenotype of this endangered species. Importantly, these results not only provide guidance for the development of conservation plans but also enhance our understanding of evolutionary past for this and other endangered species with similar histories.

关键词： Davidia involucrata Cryptic lineage Hybridization Population genomics Positive evolution

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低浓度异丙醇对口腔门诊污染物表消毒效果的临床评价

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山西医药杂志 2024年第13期53卷 968-973页

作者：范丽丽刘艳艳曹瑞锋杨旭晨杜晶丽张楠山西白求恩医院同济山西医院口腔科 030032

目的评价低浓度异丙醇与常规消毒剂对口腔治疗所致污染物表的清洁消毒效果。方法使用灭菌蒸馏水(阳性对照组)、75%乙醇溶液(乙醇组)、500 mg/L含氯消毒液(500 mg/L含氯组)、1000 mg/L含氯消毒液(1000 mg/L含氯组)、季铵盐化合物消毒湿巾... 详细信息

目的评价低浓度异丙醇与常规消毒剂对口腔治疗所致污染物表的清洁消毒效果。方法使用灭菌蒸馏水(阳性对照组)、75%乙醇溶液(乙醇组)、500 mg/L含氯消毒液(500 mg/L含氯组)、1000 mg/L含氯消毒液(1000 mg/L含氯组)、季铵盐化合物消毒湿巾(QACs组)、17.2%异丙醇+0.28%苄索氯铵消毒液(IACs组)对12例男性患者洁治过程中污染的玻璃标本进行清洁消毒。采用压印法在血琼脂平板培养基培养消毒标本表面残留微生物并计数。使用χ^(2)分割法检验不同消毒剂的清洁效果差异;使用单因素方差分析检验不同消毒剂的消毒效果。结果QACs组和IACs组标本表面未见有血液、黏液或其他可见污染物的残留,与其他4组差异有统计学意义(P0.05)。QACs组(0.05±0.01)和IACs组(0.04±0.01)物表菌落总数(TSC)差异无统计学意义(P>0.05),而这2组TSC均小于500 mg/L含氯组(21.88±2.79)、乙醇组(8.21±1.08)及1000 mg/L含氯组(2.96±0.82),差异有统计学意义(P<0.05);500 mg/L含氯组、乙醇组及1000 mg/L含氯组3组TSC依次减少,且差异有统计学意义(P<0.05)。结论QACs和IACs消毒剂对口腔治疗过程中的污染物表具有良好的清洁消毒效果。

关键词： 2-丙醇消毒药(剂) 感染控制口腔治疗

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Recent Developments on the H_(I)Gas of Low-Redshift Galaxies Seen by the 21 cm Emission Lines

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Chinese Physics Letters 2024年第10期41卷 152-165页

作者： Jing Wang xuchen Lin Kavli Institute for Astronomy and Astrophysics Peking UniversityBeijing 100871China Department of Astronomy School of PhysicsPeking UniversityBeijing 100871China

As a major interstellar medium,the atomic neutral hydrogen(H I)plays an important role in the galaxy *** provides the ingredient for star formation,and sensitively traces the internal processes and external perturbations influencing the *** the beginning of many new radio telescopes and surveys,H_(I)may make a more significant contribution to the understanding of galaxies in the near *** review discusses the major development of the 21 cm emission-line H_(I)observations and studies in the past few years,including its scaling relations with other galaxy properties,its kinematics and structures,its role in environmental studies,and its constraints on hydrodynamical *** local-Universe H_(I)scaling relations of stellar-massselected samples extend smoothly to 10_(9)M⊙stellar mass,with a tentative evolution to the redshift of∼*** development of measurement techniques enables better estimations of H_(I)non-circular motion,dispersion,and thickness,and new observations revealed extended or extra-planar H_(I)structures,both helpfully constraining the gas accretion,stellar feedback,and star formation processes of galaxy evolution models.H_(I)is very useful for tracing the satellite evolution in dense environments,the studies of which would benefit from ongoing blind H_(I)*** simulations still cannot fully reproduce H_(I)gas properties,they help to understand the role of possible factors in regulating H_(I)properties.

关键词： evolution. galaxy stellar

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大豆花药优势表达基因GmFLA22a调控雄性育性的功能研究

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遗传 2024年第4期46卷 333-345页

作者：曹振林李金红周铭辉张曼婷王宁陈一飞李嘉欣祝青松宫雯珺杨绪晨方小龙和家贤李美娜广州大学分子遗传与进化创新研究中心广东省植物适应性与分子设计重点实验室广州510006

我国大豆对外依赖度高,加速提高大豆产量是目前亟需解决的问题。利用杂种优势是大幅提高作物产量的有效途径之一,近年来基于隐性核不育基因开发的智能雄性不育系统,为快速利用大豆杂种优势提供了可能。但是,大豆雄性不育基因研究相对滞... 详细信息

我国大豆对外依赖度高,加速提高大豆产量是目前亟需解决的问题。利用杂种优势是大幅提高作物产量的有效途径之一,近年来基于隐性核不育基因开发的智能雄性不育系统,为快速利用大豆杂种优势提供了可能。但是,大豆雄性不育基因研究相对滞后。本研究基于课题组大豆花器官转录组数据,筛选到在大豆早期花药中优势表达基因GmFLA22a,编码含有FAS1结构域的成束状阿拉伯半乳糖蛋白,亚细胞定位表明其可能在内质网中发挥功能。利用基因编辑技术获得Gmfla22a突变体,突变体植株在营养生长阶段与对照组相比没有明显差异,但在生殖生长阶段表现为结实率显著降低。Gmfla22a突变体花粉活力和花粉萌发率均无明显异常,组织切片并染色观察发现,突变体植株花药室壁增厚,花粉粒释放延迟、不完全,这可能是导致Gmfla22a结实率降低的原因。综上,本研究初步揭示GmFLA22a可能参与调控大豆雄性育性,为深入揭示其分子功能提供重要遗传材料,同时为大豆杂种优势利用提供基因资源和理论依据。

关键词：大豆杂种优势雄性不育基因编辑反向遗传学

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Controlled Synthesis of Graphdiyne-Based Multiscale Catalysts for Energy Conversion

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Precision Chemistry 2024年第7期2卷 355-375页

作者： Siao Chen xuchen Zheng yang Gao Xinyu Ping Yurui Xue Yuliang Li CAS Key Laboratory of Organic Solids Institute of Chemistry Chinese Academy of SciencesBeijing 100190China Shandong Provincial Key Laboratory for Science of Material Creation and Energy Conversion Science Center for Material Creation and Energy ConversionSchool of Chemistry and Chemical EngineeringShandong UniversityJinan 250100China School of Chemical Sciences University of Chinese Academy of SciencesBeijing 100049China

Graphdiyne(GDY)science is a new and rapidly developing interdisciplinary field that touches on various areas of chemistry,physics,information science,material science,life science,environmental science,and so *** rapid development of GDY science is part of the trend in development of carbon ***,with its unique structure and fascinating properties,has greatly promoted fundamental research toward practical applications of carbon *** important applications,such as catalysis and energy conversion,have been *** particular,GDY has shown great potential for application in the field of *** have precisely synthesized a series of GDY-based multiscale catalysts and applied them in various energy conversion and catalysis research,including ammonia synthesis,hydrogen production,CO_(2) conversion,and chemical-to-electrical energy *** this paper,we systematically review the advances in the precisely controlled synthesis of GDY and aggregated structures,and the latest progress with GDY in catalysis and energy conversion.

关键词： graphdiyne controlled growth atom catalyst heterostructure energy conversion

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POX1 regulates the formation of viable but non-culturable brewer’s yeast induced by iso-alpha acid from hops

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Food Science and Human Wellness 2024年

作者： Zengyan Wang yang Xiao xuchen Li Zhenqing Li Yan Wang Wei He Jiayang Wang Li Liu Ting Ding Pengdong Sun Jingyuan Li yang Deng Shandong Technology Innovation Center of Special Food 4. Qingdao Special Food Research Institute Key Laboratory of Special Food Processing (Co-construction by Ministry and Province)， Ministry of Agriculture Rural Affairs， Qingdao Agricultural University School of Food Engineering， Qingdao Institute of Technology College of Food Science and Engineering， Qingdao Agricultural University Shandong Huifa Foodstuff Co.， Ltd.

In this study， we utilized gene knockout and overexpression techniques to generate brewer’s yeast strains with either a deletion or overexpression of the POX1 gene. The strains studied included the parental strain， the POX1 deletion strain， and the POX1 overexpression strain. These strains were exposed to iso-alpha acid from hops at a concentration of 300 mg/L， leading to the induction of a viable but non-culturable (VBNC) state. Our results indicated that the silencing of the POX1 gene rendered brewer’s yeast cells unable to withstand the high concentration of iso-alpha acid stress， ultimately leading to cell death. Conversely， the overexpression of POX1 accelerated the transition of yeast cells into the VBNC state compared to the parental strain. Furthermore， we evaluated the levels of reactive oxygen species (ROS)， catalase (CAT) activity， superoxide dismutase (SOD) activity， glutathione reductase (GR) activity， and the mRNA expression of genes that regulate these enzymes (SYM1， CTA1， SOD1， and GLR1) in brewer’s yeast cells at three distinct stages: normal， short-term stress， and VBNC states. Based on these findings， it can be inferred that the formation of the VBNC state in brewer’s yeast is associated with the response to oxidative stress.

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Insight into the effect of geographic location and intercropping on contamination characteristics and exposure risk of phthalate esters(PAEs) in tea plantation soils

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Journal of Integrative Agriculture 2024年第11期23卷 3896-3911页

作者： Jie Li Shanjie Han Ruhang Xu xuchen Zhang Junquan Liang Mengxin Wang Baoyu Han Zhejiang Provincial Key Laboratory of Biometrology and Inspection and Quarantine College of Life SciencesChina Jiliang University Hangzhou Tea & Chrysanthemum Technology Co.Ltd.

Phthalate esters(PAEs) are an emerging pollutant due to widespread distribution in environmental mediums that have attracted widespread attention over recent years. However, there is little information about tea plantation soil PAEs. A total of 270 soil samples collected from 45 tea plantations in the major high-quality tea-producing regions of Jiangsu, Zhejiang, and Anhui provinces in China were analyzed for seven PAEs. The detection frequency of PAEs in tea plantation soil was 100%. DBP, DEHP, and DiBP were the main congeners in tea plantation soil. The PAEs concentrations in the upper soil were significantly higher than those in the lower soil. The concentration of tea plantation soil PAEs in Jiangsu Province was significantly lower than those in Zhejiang and Anhui provinces. Intercropping with chestnuts can effectively reduce the contamination level of PAEs in tea plantation soil. Correlation analysis, redundancy analysis, partial correlation analysis, and structural equation modeling methods further confirmed the strong direct influence of factors such as chestnut–tea intercropping, temperature, and agricultural chemicals on the variation of PAEs in tea plantation soil. The health and ecological risk assessments indicated that non-carcinogenic risk was within a safe range and that there was a high carcinogenic risk via the dietary pathway, with DBP posing the highest ecological risk.

关键词： phthalate esters chestnut and tea intercropping driving factors spatial heterogeneity risk assessment

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Computational and dark-field ghost imaging with ultraviolet light

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Photonics Research 2024年第2期12卷 226-234页

作者： JIAQI SONG BAOLEI LIU YAO WANG CHAOHAO CHEN xuchen SHAN XIAOLAN ZHONG LING-AN WU FAN WANG School of Physics Beihang UniversityBeijing 102206China Australian Research Council Centre of Excellence for Transformative Meta-Optical Systems Department of Electronic Materials EngineeringResearch School of PhysicsThe Australian National UniversityCanberraACT 2600Australia Institute of Physics Chinese Academy of SciencesBeijing 100190China

Ultraviolet(UV)imaging enables a diverse array of applications,such as material composition analysis,biological fluorescence imaging,and detecting defects in semiconductor ***,scientific-grade UV cameras with high quantum efficiency are expensive and include complex thermoelectric cooling ***,we demonstrate a UV computational ghost imaging(UV-CGI)method to provide a cost-effective UV imaging and detection *** applying spatial–temporal illumination patterns and using a 325 nm laser source,a singlepixel detector is enough to reconstruct the images of *** use UV-CGI to distinguish four UV-sensitive sunscreen areas with different densities on a ***,we demonstrate dark-field UV-CGI in both transmission and reflection *** only collecting the scattered light from objects,we can detect the edges of pure phase objects and small scratches on a compact *** results showcase a feasible low-cost solution for nondestructive UV imaging and *** combining it with other imaging techniques,such as hyperspectral imaging or time-resolved imaging,a compact and versatile UV computational imaging platform may be realized for future applications.

关键词： ghost illumination versatile

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考虑电动汽车有序充电的光储充电站储能容量优化策略

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电力系统保护与控制 2021年第7期49卷 94-102页

作者：李景丽时永凯张琳娟杨旭晨王利利陈星郑州大学电气工程学院河南郑州450001 国网河南省电力公司经济技术研究院河南郑州450000 国网河南省电力公司商丘供电公司河南商丘476000

针对电动汽车和光伏系统接入配电网与储能装置结合过程中的配置优化问题,提出了一种考虑电动汽车有序充电的光储充电站储能容量优化策略。首先,基于典型日光照强度曲线和光电能量转换关系计算光伏系统输出功率。其次,根据电动汽车用户... 详细信息

针对电动汽车和光伏系统接入配电网与储能装置结合过程中的配置优化问题,提出了一种考虑电动汽车有序充电的光储充电站储能容量优化策略。首先,基于典型日光照强度曲线和光电能量转换关系计算光伏系统输出功率。其次,根据电动汽车用户出行习惯、充电行为特性、充电模式等充电负荷影响因素,建立影响电动汽车充电负荷的概率模型,利用蒙特卡洛方法预测无序充电下电动汽车充电负荷。然后,以电网出力曲线峰谷差最小为目标函数、采用粒子群算法计算电动汽车有序充电时电网出力总负荷,进而确定光储充电站储能容量最优解。最后,利用所提策略计算以电动私家车和电动出租车为主要服务对象的某居民区光储充电站内最优储能容量。结果表明,未考虑储能时电动汽车无序充电造成电网负荷峰上加峰,有序充电下电网负荷峰谷差值下降15.35%,考虑电动汽车有序充电同时配置最优储能容量时电网负荷峰谷差值下降了20.65%,实现了削峰填谷,增强了电力系统运行的稳定性。得到的结果为光储充电站的储能容量配置提供了参考。

关键词：电动汽车有序充电光储充电站容量优化粒子群算法

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A reduced graphene oxide-coated conductive surgical silk suture targeting microresistance sensing changes for wound healing

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Science China Technological Sciences 2024年第11期67卷 3499-3512页

作者： DING YuQi WANG xuchen LIU JingGe SHEN HongQiang WANG Zhong XIE MaoBin CHEN Ying BARCENAS Adileidys Ruiz ZHAO ZeYu LI Gang National Engineering Laboratory for Modern Silk College of Textile and Clothing EngineeringSoochow University The Sixth Affiliated Hospital of Guangzhou Medical University Qingyuan People's Hospital School of Biomedical Engineering Guangzhou Medical University Department of Biomedical Engineering Tufts University National Sericulture Project Research Center in Plant Proteins and Bionatural Products Department of Applied Physics The Hong Kong Polytechnic University

Conventional sutures used in surgical procedures often lack the capability to effectively monitor physical and chemical activities or the microbial environment of surgical wounds due to their inadequate mechanical properties, insufficient electrical accuracy and unstability. Here, we present a straightforward layer-by-layer coating technique that utilizes 3-glycidoxypropyltrimethoxysilane(CA), graphene oxide(GO), and ascorbic acid(AA) to develop conductive silk-based surgical sutures(CA-rGSFS). The CA-rGSFS feature a continuous reduced graphene oxide(rGO) film on their surface, forming robust hydrogen bonds with silk fibroin. The reduction process of rGO is confirmed through Raman analysis, demonstrating an enhanced D peak to G peak ***, the CA-rGSFS exhibit exceptional mechanical properties and efficient electron transmission, with a knot-pull tensile strength of 2089.72 ± 1.20 cN and an electrical conductivity of 130.30 ± 11.34 S/m, respectively, meeting the requirements specified by the United States Pharmacopeia(USP) for 2-0 sutures. These novel CA-rGSFS demonstrate the ability to accurately track resistance changes in various fluid environments with rapid response, including saline, intestinal, and gastric fluids. The suture also retains remarkable stretchablility and stability even after enduring 3000 tensile cycles, highlighting their potential for precise surgical site monitoring during the wound healing process.

关键词： layer-by-layer coating reduced graphene oxide silk suture electrical conductivity microresistance sensing wound monitoring

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