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Efficient nanoparticle-based CRISPR-Cas13d induced mRNA disruption of an eye pigmentation gene in the white-backed planthopper,Sogatella furcifera

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Insect Science 2023年第6期30卷 1552-1564页

作者： Yun-Feng Ma Meng-Qi Zhang Lang-Lang Gong xuan-zheng liu Gui-Jun Long Huan Guo J.Joe Hull Youssef Dewer Ming He Peng He National Key Laboratory of Green Pesticide Key Laboratory of Green Pesticide and Agricultural BioengineeringMinistry of EducationCenter for R&D of Fine Chemicals of Guizhou UniversityGuiyangChina USDA-ARS Arid Land Agricultural Research Center MaricopaAZUSA Phytotoxicity Research Department Central Agricultural Pesticide LaboratoryAgricultural Research CenterDokkiGizaEgypt

The discovery of the clustered regularly interspaced short palindromic repeat(CRISPR)system has driven gene manipulation technology to a new era with applications reported in organisms that span the tree of *** utility of CRISPR-mediated editing was further expanded to mRNA following identification of the RNA-targeting Cas13 family of smaller endonuclease *** of this family to insect research,however,has been more *** this study,the smallest Cas13 family member,Cas13d,and guide RNAs(gRNAs)were complexed with a versatile nanomaterial(star polycation,SPc)to generate a proof-of-concept RNA-editing platform capable of disrupting mRNA expression of the eye pigmentation gene tryptophan 2,3-dioxygenase(SfTO)in white-backed planthoppers(WBPHs).The resulting red-eye phenotype was present in 19.76%(with SPc)and 22.99%(without SPc)of the treatment groups and was comparable to the red-eye phenotype generated following conventional RNA interference knockdown(22.22%).Furthermore,the Cas13/gRNA phenotype manifested more quickly than RNA *** with the expected Cas13d mechanism,SfTO transcript levels were significantly *** together,the results indicate that the SPc-CRISPR-Cas13d/gRNA complex negatively impacted expression of the target *** findings confirm the utility of this novel mRNA disruption system in insects and lay the foundation for further development of these tools in the implementation of green agricultural pest management tactics.

关键词： CRISPR-Cas13d RNA editing tryptophan 2,3-dioxygenase white-backed planthoppers

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Efficient DIPA-CRISPR-mediated knockout of an eye pigment gene in the white-backed planthopper,Sogatella furcifera

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Insect Science 2024年第4期31卷 1015-1025页

作者： Meng-Qi Zhang Lang-Lang Gong Ya-Qin Zhao Yun-Feng Ma Gui-Jun Long Huan Guo xuan-zheng liu J.Joe Hull Youssef Dewer Chao Yang Ning-Ning Zhang Ming He Peng He National Key Laboratory of Green Pesticides Key Laboratory of Green Pesticide and Agricultural BioengineeringMinistry of EducationCenter for R&D of Fine Chemicals of Guizhou UniversityGuiyangChina Pest Management and Biocontrol Research Unit US Arid Land Agricultural Research CenterUSDA Agricultural Research ServicesMaricopaArizonaUSA Phytotoxicity Research Department Central Agricultural Pesticide LaboratoryAgricultural Research CenterDokkiGizaEgypt Guizhou Jifeng Seed Industry Limited Liability Company XingyiGuizhou ProvinceChina Shandong Facility Horticulture Bioengineering Research Center Weifang University of Science and TechnologyWeifangShandong ProvinceChina

Although CRISPR/Cas9 has been widely used in insect gene editing,the need for the microinjection of preblastoderm embryos can preclude the technique being used in insect species with eggs that are small,have hard shells,and/or are difficult to collect and maintain outside of their normal *** is the case with Sogatella furcifera,the white-backed planthopper(WBPH),a significant pest of Oryza sativa(rice)that oviposits inside rice *** extraction from the stem runs the risk of mechanical damage and hatching is heavily influenced by the micro-environment of the rice *** bypass these issues,we targeted embryos prior to oviposition via direct parental(DIPA)-CRISPR,in which Cas9 and single-guide RNAs(sgRNAs)for the WBPH eye pigment gene tryptophan 2,3-dioxygenase were injected into the hemocoel of adult *** at varying numbers of days posteclosion were evaluated to determine at what stage their oocyte might be most capable of taking up the gene-editing *** evaluation of the offspring indicated that the highest G0 gene-edited efficacy(56.7%)occurred in females injected 2 d posteclosion,and that those mutations were heritably transmitted to the G1 *** study demonstrates the potential utility of DIPA-CRISPR for future gene-editing studies in non-model insect species and can facilitate the development of novel pest management applications.

关键词： CRISPR/Cas9 DIPA-CRISPR gene editing tryptophan 2,3-dioxygenase WBPH

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Three-dimensional fracture of UO2 ceramic pellets by phase field modeling

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Science China(Physics,Mechanics & Astronomy) 2025年第1期Mechanics & Astronomy) .卷 169-180页

作者： Wei Xiong xuan Ye Hongzhang Cheng Xiaoming liu Institute of Nuclear and New Energy Technology Collaborative Innovation Center of Advanced Nuclear Energy Technology Key Laboratory of Advanced Reactor Engineering and Safety of Ministry of Education Tsinghua University State Key Laboratory of Nonlinear Mechanics Institute of Mechanics Chinese Academy of Sciences School of Engineering Science University of Chinese Academy of Sciences

Fracture is the primary failure type for the UO2ceramic pellets,which are operated under irradiation and thermal processes,and physically confined by the *** this work,by combining closely coupled physical processes of heat conduction,heat transfer,and mechanical deformation with the cohesive phase field framework,we proposed a high-precision modeling of quasistatic cracking of three-dimensional （3D） UO2ceramic fuel pellets under high temperature and *** simulation results agree well with the experimental results,indicating that the proposed method has the potential to capture 3D crack modes in the interested time ***,we studied the relation of the 3D fracture patterns of fuel pellets with the peak power *** was found that the power level plays a critical role in determining the competition between radial and circumferential cracks,as well as transverse penetrating cracks.

关键词： phase field method nuclear fuel pellet coupled thermo-mechanical model thermal fracture

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The role of axon guidance molecules in the pathogenesis of epilepsy

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Neural Regeneration Research 2025年第5期20卷 1244-1257页

作者： zheng liu Chunhua Pan Hao Huang Department of Neurology Affiliated Hospital of Zunyi Medical UniversityZunyiGuizhou ProvinceChina

Current treatments for epilepsy can only manage the symptoms of the condition but cannot alter the initial onset or halt the progression of the disease. Consequently, it is crucial to identify drugs that can target novel cellular and molecular mechanisms and mechanisms of action. Increasing evidence suggests that axon guidance molecules play a role in the structural and functional modifications of neural networks and that the dysregulation of these molecules is associated with epilepsy susceptibility. In this review, we discuss the essential role of axon guidance molecules in neuronal activity in patients with epilepsy as well as the impact of these molecules on synaptic plasticity and brain tissue remodeling. Furthermore, we examine the relationship between axon guidance molecules and neuroinflammation, as well as the structural changes in specific brain regions that contribute to the development of epilepsy. Ample evidence indicates that axon guidance molecules, including semaphorins and ephrins, play a fundamental role in guiding axon growth and the establishment of synaptic connections. Deviations in their expression or function can disrupt neuronal connections, ultimately leading to epileptic seizures. The remodeling of neural networks is a significant characteristic of epilepsy, with axon guidance molecules playing a role in the dynamic reorganization of neural circuits. This, in turn, affects synapse formation and elimination. Dysregulation of these molecules can upset the delicate balance between excitation and inhibition within a neural network, thereby increasing the risk of overexcitation and the development of epilepsy. Inflammatory signals can regulate the expression and function of axon guidance molecules, thus influencing axonal growth, axon orientation, and synaptic plasticity. The dysregulation of neuroinflammation can intensify neuronal dysfunction and contribute to the occurrence of epilepsy. This review delves into the mechanisms associated with

关键词： axon guidance drug-resistant epilepsy epilepsy nerve regeneration nervous system diseases neural pathways neuroinflammatory diseases neuronal plasticity neurons synaptic remodeling

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Multi boron-doping effects in hard carbon toward enhanced sodium ion storage

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Journal of Energy Chemistry 2025年第1期100卷 730-738页

作者： Peng zheng Wang Zhou Ying Mo Biao zheng Miaomiao Han Qin Zhong Wenwen Yang Peng Gao Lezhi Yang Jilei liu College of Materials Science and Engineering Hunan Joint International Laboratory of Advanced Materials and Technology for Clean Energy Hunan Province Key Laboratory for Advanced Carbon Materials and Applied Technology Hunan University Greater Bay Area Institute for Innovation Hunan University Changsha Research Institute of Mining & Metallurgy Co. LTD.

Hard carbon （HC） has been considered as promising anode material for sodium-ion batteries （SIBs）.The optimization of hard carbon’s microstructure and solid electrolyte interface （SEI） property are demonstrated effective in enhancing the Na+storage capability,however,a one-step regulation strategy to achieve simultaneous multi-scale structures optimization is highly ***,we have systematically investigated the effects of boron doping on hard carbon’s microstructure and interface chemistry.A variety of structure characterizations show that appropriate amount of boron doping can increase the size of closed pores via rearrangement of carbon layers with improved graphitization degree,which provides more Na+storage ***-situ Fourier transform infrared spectroscopy/electrochemical impedance spectroscopy （FTIR/EIS） and X-ray photoelectron spectroscopy （XPS） analysis demonstrate the presence of more BC3and less B–C–O structures that result in enhanced ion diffusion kinetics and the formation of inorganic rich and robust SEI,which leads to facilitated charge transfer and excellent rate *** a result,the hard carbon anode with optimized boron doping content exhibits enhanced rate and cycling *** general,this work unravels the critical role of boron doping in optimizing the pore structure,interface chemistry and diffusion kinetics of hard carbon,which enables rational design of sodium-ion battery anode with enhanced Na+storage performance.

关键词： X ray photoelectron spectroscopy

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Increased excitatory amino acid transporter 2 levels in basolateral amygdala astrocytes mediate chronic stress–induced anxiety-like behavior

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Neural Regeneration Research 2025年第6期20卷 1721-1734页

作者： Xirong Xu Shoumin xuan Shuai Chen Dan liu Qian Xiao Jie Tu Shenzhen Key Laboratory of Neuroimmunomodulation for Neurological Diseases Shenzhen-Hong Kong Institute of Brain ScienceShenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhenGuangdong ProvinceChina CAS Key Laboratory of Brain Connectome and Manipulation Brain Cognition and Brain Disease Institute(BCBDI)Shenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhenGuangdong ProvinceChina Guangdong Provincial Key Laboratory of Brain Connectome and Behavior the Brain Cognition and Brain Disease Institute(BCBDI)Shenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhenGuangdong ProvinceChina University of Chinese of Academy of Sciences BeijingChina Faculty of Life and Health Sciences Shenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhenGuangdong ProvinceChina

The conventional perception of astrocytes as mere supportive cells within the brain has recently been called into question by empirical evidence, which has revealed their active involvement in regulating brain function and encoding behaviors associated with ***, astrocytes in the basolateral amygdala have been found to play a role in the modulation of anxiety-like behaviors triggered by chronic stress. Nevertheless, the precise molecular mechanisms by which basolateral amygdala astrocytes regulate chronic stress–induced anxiety-like behaviors remain to be fully elucidated. In this study, we found that in a mouse model of anxiety triggered by unpredictable chronic mild stress, the expression of excitatory amino acid transporter 2 was upregulated in the basolateral amygdala. Interestingly, our findings indicate that the targeted knockdown of excitatory amino acid transporter 2 specifically within the basolateral amygdala astrocytes was able to rescue the anxiety-like behavior in mice subjected to stress. Furthermore, we found that the overexpression of excitatory amino acid transporter 2 in the basolateral amygdala, whether achieved through intracranial administration of excitatory amino acid transporter 2agonists or through injection of excitatory amino acid transporter 2-overexpressing viruses with GfaABC1D promoters, evoked anxiety-like behavior in mice. Our single-nucleus RNA sequencing analysis further confirmed that chronic stress induced an upregulation of excitatory amino acid transporter 2 specifically in astrocytes in the basolateral amygdala. Moreover, through in vivo calcium signal recordings, we found that the frequency of calcium activity in the basolateral amygdala of mice subjected to chronic stress was higher compared with normal *** knocking down the expression of excitatory amino acid transporter 2 in the basolateral amygdala, the frequency of calcium activity was not significantly increased, and anxiety-like behavior was ob

关键词： anxiety astrocytes basolateral amygdala behavior dihydrokainic acid excitatory amino acid transporter 2 fiber photometry glutamate LDN-212320 transporter

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Influence of introducing Zr,Ti,Nb and Ce elements on externally solidified crystals and mechanical properties of high-pressure die-casting Al–Si alloy

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International Journal of Minerals,Metallurgy and Materials 2025年第1期32卷 147-153页

作者： Junjie Li Wenbo Yu Zhenyu Sun Weichen zheng Liangwei Zhang Yanling Xue Wenning liu Shoumei Xiong Center of Materials Science and Engineering School of Mechanical and Electronic Control EngineeringBeijing Jiaotong UniversityBeijing 100044China Traffic&Transportation Engineering Central South UniversityChangsha 410075China Shanghai Synchrotron Radiation Facility Shanghai Advanced Research InstituteChinese Academy of SciencesShanghai 201204China School of Materials Science and Engineering Tsinghua UniversityBeijing 100084China

High pressure die casting(HPDC)AlSi10Mn Mg alloy castings are widely used in the automobile *** can optimize the mechanical properties of castings through heat treatment,while the release of thermal stress arouses the deformation of large integrated ***,the development of non-heat treatment Al alloys is becoming the hot *** addition,HPDC contains externally solidified crystals(ESCs),which are detrimental to the mechanical properties of *** achieve high strength and toughness of non-heat treatment die-casting Al-Si alloy,we used AlSi9Mn alloy as matrix with the introduction of Zr,Ti,Nb,and *** influences on ESCs and mechanical properties were systematically investigated through three-dimensional reconstruction and thermodynamic *** results reveal that the addition of Ti increased ESCs'size and porosity,while the introduction of Nb refined ESCs and decreased ***,large-sized Al_3(Zr,Ti)phases formed and degraded the mechanical *** introduction of Ce resulted in the poisoning effect and reduced mechanical properties.

关键词： aluminium alloy high-pressure die-casting externally solidified crystals non-heat treatment

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Near-perfect replication on amorphous alloys through active force modulation based on machine learning/neural network parameter prediction

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Science China(Physics,Mechanics & Astronomy) 2025年第1期Mechanics & Astronomy) .卷 181-191页

作者： Senkuan Meng zheng Wang Ruisong Zhu Ruijie liu Jiang Ma Lina Hu Weihua Wang Key Laboratory for Liquid-Solid Structural Evolution and Processing of Materials (Ministry of Education) School of Materials Science and Engineering Shandong University College of Mechatronics and Control Engineering Shenzhen University Songshan Lake Materials Laboratory Institute of Physics Chinese Academy of Sciences

As a microforming technique, micro/nano-structural replication possesses advantages of high precision and efficiency. With the remarkable superplasticity in the supercooled liquid region, amorphous alloys or metallic glasses(MGs) are regarded as ideal materials for miniature fabrication. However, due to the intrinsic metastable nature of supercooled liquids, the design of imprinting processes for MGs poses a challenge. In the past, process parameters have largely relied on trial-and-error *** this work, a low-frequency active force modulation system is employed to apply a stable, precise, and real-time feedback stress field for imprinting of MG samples. Low-frequency vibrations can facilitate the filling of microstructures on the template surface by reducing the effective viscosity of the supercooled liquid. With the dataset composed of orthogonal experiments, a machine learning strategy based on back-propagation(BP) neural networks was utilized to construct a 3D parameter space for temperature, stress, and time, and to predict the corresponding filling ratio. Furthermore, the optimal combination of imprinting process parameters was identified, and its filling ratio was experimentally validated to reach as high as 0.94. The near-perfect replication of microstructures confirms the superiority of the active force modulation system and the data-driven strategy of machine learning-assisted parameter design. At the same time, this one-step microforming process provides a new approach to addressing the accuracy-cost trade-off dilemma in precision manufacturing.

关键词： metallic glass amorphous alloy microforming imprinting machine learning

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Endogenous/exogenous dual stimulation“ROS engineering”amplification combined with autophagy-augmented for efficient ferroptosis therapy

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Nano Research 2025年第1期18卷 597-606页

作者： Pengye Du Pengpeng Lei Yuan Liang Ran An Yi Wei Shuyu liu Jianhao zheng Hongjie Zhang State Key Laboratory of Rare Earth Resource Utilization Changchun Institute of Applied ChemistryChinese Academy of SciencesChangchun 130022China School of Applied Chemistry and Engineering University of Science and Technology of ChinaHefei 230026China Department of Chemistry Tsinghua UniversityBeijing 100084China

“Reactive oxygen species(ROS)engineering”is one of the most promising anti-tumor treatments developed in recent *** explosion of ROS will cause oxidative stress,thereby inducing tumor cell ***,ROS accumulation in tumor cells is eliminated by endogenous cellular self-regulation *** this work,a kind of endogenous/exogenous dual stimulation nanoagent noted as UMZC is *** reaction occurs between NH_(2)-MIL-88B(Fe)contained in UMZC and the overexpression of endogenous H_(2)O_(2)to generate ROS,amplified by endogenous H_(2)S of colon tumor *** addition,NH_(2)-MIL-88B(Fe)also functions to deplete glutathione(GSH)for stopping it from consuming *** nanoparticles at the core of UMZC convert near-infrared into visible light,which excites zinc phthalocyanine to initiate the photochemical reaction that generates more ROS,thus alleviating the lack of tissue penetration depth for visible *** agonist chitosan oligosaccharides induce enhancement of cellular autophagy for disrupting cellular metabolic stress and the resistance to oxidative stress of tumor cells,allowing“ROS engineering”to fully exert anti-tumor *** of ROS generation,GSH depletion,and induced cellular autophagy caused by nanoagents have promoting effects on the occurrence of ***,the nanoagents show the ability to effectively treat colon tumors.

关键词： reactive oxygen species(ROS)engineering upconversion nanoparticles cellular autophagy ferroptosis tumor therapy

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张家界森林大气中醛酮类化合物浓度变化特征

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环境科学研究 2016年第9期29卷 1272-1278页

作者：蒋朝晖王玉娇郑玄刘湘珍夏畅斌肖炘圻黎莎长沙理工大学化学与生物工程学院湖南长沙410114 张家界武陵源妇联湖南张家界427400

为探究中亚热带森林大气中醛酮类化合物浓度变化特征,于2014年8月—2015年1月在张家界森林公园采用EPA TO-11A方法对张家界森林大气中醛酮类化合物质量浓度进行了监测.结果表明:张家界森林大气中主要的醛酮类化合物为甲醛、乙醛、丙酮... 详细信息

为探究中亚热带森林大气中醛酮类化合物浓度变化特征,于2014年8月—2015年1月在张家界森林公园采用EPA TO-11A方法对张家界森林大气中醛酮类化合物质量浓度进行了监测.结果表明:张家界森林大气中主要的醛酮类化合物为甲醛、乙醛、丙酮、丙醛和MACR(甲基丙烯醛),质量浓度分别为4.32、0.95、3.01、0.48和0.51μg/m3.大气中ρ(甲醛)、ρ(乙醛)和ρ(MACR)的季节变化特征很明显,夏季和秋季醛酮类化合物质量浓度较高,冬季醛酮类化合物质量浓度较低.此外,醛酮类化合物质量浓度日变化显著,除了10月、12月受人为因素影响较大外,其余月份醛酮质量浓度最大值通常出现在13:00—15:00.张家界森林大气中C1/C2(ρ(甲醛)/ρ(乙醛))为5.72,比城市地区(C1/C2为1左右)高,但比偏远森林地区(C1/C2为10左右)低.ρ(甲醛)与ρ(乙醛)、ρ(MACR)均呈正相关且达到显著水平,而ρ(丙酮)与ρ(甲醛)、ρ(乙醛)与ρ(MACR)的相关性差.与文献报道的加拿大Ontario、墨西哥Langmuir等地区对比,张家界森林大气中醛酮类化合物质量浓度较高,但明显低于北京、上海等城市地区.研究显示,张家界森林大气中甲醛、乙醛和MACR主要来自森林地区植物排放的VOCs光氧化生成,丙酮除了来自植物排放的有机物光氧化分解外,还有其他人为源,进一步说明了张家界森林大气中醛酮类化合物浓度变化主要受光化学反应等自然影响,但人为因素的影响也不容忽略.

关键词：醛酮类化合物光氧化反应张家界森林大气

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